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All Journal Agriprima : Journal of Applied Agricultural Sciences Jurnal Pengembangan Potensi Laboratorium
Riani Ningsih
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Engineering

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Pengaruh Cekaman Suhu Tinggi pada Fase Bibit terhadap Pertumbuhan dan Hasil Umbi Dua Varietas Tanaman Kentang (Solanum Tuberosum L.) Riani Ningsih; Slameto; Ketut Anom Wijaya
Agriprima : Journal of Applied Agricultural Sciences Vol 5 No 2 (2021): SEPTEMBER
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/agriprima.v5i2.390

Abstract

Kentang umumnya dapat tumbuh optimal di dataran tinggi, ketika kentang ditanam di dataran medium maka pertumbuhan tanaman akan terganggu. Hal ini dikarenakan pada dataran medium memiliki suhu yang relative tinggi. Oleh karena itu perlu dilakukan penelitian untuk mengetahui respon tanaman kentang jika ditanam pada suhu tinggi. Penelitian ini bertujuan untuk mengetahui pengaruh cekaman suhu tinggi pada fase bibit terhadap pertumbuhan dan hasil pada tanaman kentang yang dilaksanakan di Jember dengan ketinggian di atas 500 mdpl (suhu rerata 320C). Penelitian ini dilaksanakan dengan menggunakan rancangan acak kelompok (RAK) faktorial yang terdiri dari dua faktor yaitu faktor pertama perlakuan suhu yaitu, Suhu Kontrol 320C (T0), Suhu 250C 16 jam dan 350C, 8 jam selama 15 hari (T1), Suhu 420C selama 8 jam (T2). Faktor kedua perlakuan varietas yaitu, varietas atlantik (V1), dan varietas granola (V2). Sehingga terdapat 6 kombinasi perlakuan dengan ulangan 4 kali. Data dianalisis menggunakan uji lanjut jarak berganda duncan 5%. Hasil penelitian interaksi antara perlakuan cekaman suhu kontrol 320C dan varietas granola kembang menunjukkan hasil terbaik pada variabel pengamatan tinggi tanaman sebesar 36,31 cm, jumlah daun sebanyak 15,95 helai, dan kandungan karbohidrat sebesar 22,49 mg/g. Sedangkan interaksi antara perlakuan cekaman suhu kontrol 320C dan varietas atlantik menunjukkan hasil terbaik pada variabel pengamatan berat umbi sebesar 17,4 g.
Pengembangan Metode Teknik Sterilisasi Eksplan Guna Meningkatkan Keberhasilan Kultur Jaringan Tanaman Stevia (Stevia Rebaudiana Bertoni) Cahyono, Eko Hadi; Riani Ningsih
Jurnal Pengelolaan Laboratorium Vol 2 No 2 (2023): Agustus
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/plp.v2i2.3685

Abstract

Stevia rebaudiana Bert is a shrub native to the borders of Paraguay, Brazil and Argentina. The leaves of this plant produce steviol glycosides, a secondary metabolite which has a sweetness level of 200-300 times sweeter than sugarcane sucrose. Conventional stevia propagation methods cannot meet the increasing need for stevia seeds or seedlings. Tissue culture is able to fulfill this deficiency because it has the potential to produce large amounts of uniform plantlets. Tissue Culture Technique is a vegetative propagation of plants. Tissue culture includes the cultivation of cells or cell aggregates, tissues and organs of plants in a medium containing sugars, vitamins, amino acids, organic salts, water, growth regulators and compacting agents. The composition of the growing medium is also very beneficial for the growth of fungi and bacteria. Therefore, explant sterilization is an important factor in determining the success of a tissue culture program. The problem is that until now there is no optimal explant sterilization technique for Stevia tissue culture. The aim of this research is to find out the best explant sterilization method from a combination of several disinfectants on Stevia (Stevia rebaudiana Bertoni) explants which can remove contaminants on the explants. The main parameters observed were Source of Contamination (descriptive: shape and color), Percentage of Explants Alive, Percentage of Contamination, Percentage of Browning, and Time when contamination first appeared (Early). The results showed that the sterilization method F used running water, immersion in detergent for 10 minutes, immersion in 1.5% fungicide solution and 1.5% bactericide for 10 minutes, 70% alcohol immersion for 1 minute, 0.05% HgCl2 immersion 3 minutes and immersion in 10% and 5% NaOCl respectively for 5 minutes had the lowest contamination percentage of 20% and the highest survival presentation of 80% with 80% fungal and 20% bacterial contaminants and the initial contamination occurred in the first week of observation.
Co-Authors Cahyono, Eko Hadi Ketut Anom Wijaya Slameto Slameto