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All Journal Pelita Perkebunan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Sulistyani Pancaningtyas
Indonesian Coffee and Cocoa Research Institute
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions

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Effectiveness of antioxidants on in vitro regeneration of Musa paradisiaca var. raja to prevent browning and enhance embryo development Aisiyya Qutlana Munawaroh, Putri; Pancaningtyas, Sulistyani; Su'udi, Mukhamad
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol. 40 No. 2 (2024)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v40i2.594

Abstract

The cultivation of raja banana is widespread in Indonesia. The process of increasing banana propagation through in vitro culture encounters a specific issue, namely the occurrence of browning during the initiation stage, which hinders the regeneration process. The objective of this study is to determine the effectivenessof antioxidants on the in vitro regeneration of raja banana to prevent browning and enhance embryo development. The study was conducted using a completely randomized design with the treatment of the antioxidant compounds ascorbic acid (AS) and melatonin (MN). The treatments consisted of control (without antioxidants), ascorbic acid (100 mg L-1, 150 mg L-1, and 200 mg L-1) and melatonin (10 mg L-1, 12 mg L-1, and 14 mg L-1). The results showed that ascorbic acid and melatonin treatment had no significant effect on the percentage of viable explants and the level of browning intensity, but provided significant results on the regeneration process. The application of melatonin at 14 mg L-1 significantly increased callus regeneration. Furthermore, at a concentration of 12 mg L-1 showed the highest callus percentage value compared to other treatments. The melatonin 12 mg L-1 treatment showed the earliest scutellar embryo formation, whereas ascorbic acid at a concentration of 100 mg L-1 resulted in the most optimal regeneration of globular and scutellar embryos. The highest concentrations of ascorbic acid and melatonin inhibit the formation of coleoptilar embryos
Potential of siam weed extract in combination with Trichoderma harzianum to stimulate seed germination of Coffea arabica Ania Ningsih, Misbahul; Pancaningtyas, Sulistyani; Su'udi, Mukhamad
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol. 40 No. 1 (2024)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v40i1.595

Abstract

The Arabica coffee (Coffea arabica) beans feature a solid and thick seed shape that obstructs the germination process. Seed germination is an important feature of coffee plant cultivation and significantly impacts on the growth and development of the coffee plant. Siam weed (Chromolaena odorata L.) has the potential to be used as an organic fertilizer due to its nutrients and secondary metabolite compounds which can help accelerate plant growth. The optimal use of organic fertilizer can be achieved by using Trichoderma harzianum. This research aims to determine the effect and optimal concentration of Siam weed extract (Chromolaena odorata L.) and Trichoderma harzianum on the germination of Arabica coffee seeds. The research used a factorial Completely Randomized Design (CRD) in the form of siam weed extract concentrations of 8 ppm and 16 ppm and concentrations of Trichoderma harzianum of 5 ppm, 10 ppm, 15 ppm, and 20 ppm. The research finding indicated that the C2T1 treatment had the best results in terms of germination, growth speed, growth synchrony, hypocotyl length, and number of leaves on coffee plants. Meanwhile, the C2T4 treatment had the highest chlorophyll content.
OPTIMIZATION OF PRIMARY SOMATIC EMBRYOGENESIS INDUCTION OFCOCOA (Theobroma cacaoL.) THROUGH THE ADDITION OF PHENOLICCOMPOUNDSUPPRESSANT MATERIALS Priandani, Putri Oktaviana; Pancaningtyas, Sulistyani; Ubaidillah, Mohammad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.1701

Abstract

Somatic embryogenesis is a propagation technique in which explants form somatic embryos. The percentage of primary somatic embryo formation in cocoa tends to be low due to the significant secretion of phenolic components in flower explants, thereby reducing the conversion rate of explants into embryos. This is indicated by browning that occurs in explants or the culture medium. The purpose of this study was to optimize primary somatic embryogenesis induction in cocoa through the addition of several types of phenolic compound suppressor materials to the culture medium. Staminodia explants were grownon DKW medium with factorial treatment: various phenolic compound suppressing materials (control; 300 mg L-1PVP; 15 mg L-1silver nitrate; 12 mg L-1melatonin; 300 mg L-1ascorbic acid) and cocoa cultivars (MCC-02 and ICCRI-09). The results showed that each material treatment produced a different response in suppressing phenolic compounds in each cultivar. The melatonin treatment resulted in the lowest percentage of browning intensity, which also had the best impact on morphology, callus size, wet weight,and the percentage of live explants.
DNA Barcoding for Identification of Helopeltis sp. from Kaliwining Experimental Station using Mitochondrial COI Gene Pancaningtyas, Sulistyani; Tunjung Sari, Ariza Budi; Rahayu, Dwi Suci
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol. 38 No. 3 (2022)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v38i3.521

Abstract

Helopeltis sp. is one of the major pests of the cocoa plant. Helopeltis can decrease cocoa yield by 50 to 60%. Several species of Helopeltis spp. are still often misidentified due to similarity in morphology such as size and color. This study aimed to identify Helopeltis sp. through DNA barcoding using the mitochondrial cyto- chrome C oxidase subunit 1 (COI) gene. Analysis was carried out on 100 adults Helopeltis sp. from Kaliwining Experimental Station, Indonesian Coffee and Cocoa Research Institute (ICCRI), Jember, Indonesia. Specimens were preserved in 96% ethyl alcohol at 4oC until molecular studies were carried out. PCR amplification was conducted through specific primer from COI gene. Sequences data analysis using MEGA 5.05 software was carried out. The study results using DNA barcoding showed that Helopeltis samples in Kaliwining cocoa plantations are Helopeltis bradyi with a percentage identity above 99% and E value of 0; this sequence as significantly matched. Mean length of the sequences was 679.5 base pairs. Phy- logenetic tree data also shows that the Helopeltis species analysed were also of the same descent, H. bradyi with the accession number KT189562.1. Helopeltis sp. specimens were clustered together. No taxonomic deviation was detected at the species level, indicating that most of the examined species of Helopeltis sp. could be authenticated by the barcode approach using the COI gene. In addi- tion, Helopeltis specimens were also aligned with H. antonii and H. theivora from the genebank to determine their genetic relationship.
Identification of cocoa (Theobroma cacao L.) genetic uniformity through RAPD molecular markers. Helia Dewi, Nur Afni; Pancaningtyas, Sulistyani; Su’udi, Mukhamad
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol. 39 No. 3 (2023)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v39i3.566

Abstract

Theobroma cacao L. has been propagated through either generative or vegetative techniques. One of the vegetative methods of propagating cocoa is somatic embryogenesis. Somatic embryogenesis has been employed by the Indonesian Coffee and Cocoa Research Institute for producing cocoa seedlings. Plant breeding activities are hampered by the significant level of plant heterogeneity among their progeny. Mislabelling of genetic impurities can be an issue. Molecular markers can be used to detect genetic variation at an early stage. The most common marker is the RAPD molecular marker. The study aims to determine the polymorphic RAPD primers in the analysis of genetic uniformity between mother plants and the seedlings derived from somatic embryogenesis (SE). The analyzed samples consisted of twelve individuals: six mother plants and six seedlings derived from SE. The results revealed that the percentage of polymorphic bands was 100% with band sizes ranging from 295-2785 bp for primer GY169 while for primer GY107 percentage of polymorphic bands was 80% with band sizes ranging from 345-1678 bp. Primer GY169 and primer GY107 can be amplified and used for cocoa similarity and heterogeneity.
Identification of Heat Shock Protein 90 (HSP90) Gene Family in Cocoa (Theobroma cacao L.) in Silico Study Mustafidah, Qori'atul; Su'udi, Mukhamad; Pancaningtyas, Sulistyani
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol. 41 No. 2 (2025)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v41i2.656

Abstract

Heat Shock Protein 90 (HSP90) has an important role in regulating heat stress response, plant development control, and defense reactions. The HSP90 gene family has been extensively reported in numerous plant species, but studies on the HSP90 gene in cocoa (Theobroma cacao L.) remain lacking. Continuous global warming and the use of low-grade quality planting materials are the reasons for low cocoa productivity in Indonesia. Based on this, it is necessary to have good adaptability in cocoa as well as the development of new cultivars so that there is no decrease in cocoa productivity. This study aims to identify the HSP90 gene family in cocoa to recognize the HSP90 functioning in cocoa and provide the necessary information to characterize HSP90 at the genome level. Identification of the HSP90 gene family in T. cacao was carried out through analysis of TcHSP90 sequences obtained from various related databases. The results showed that a total of seven TsHSP90 genes are distributed into ten T. cacao chromosomes. Analysis of the TcHSP90 motif showed that HSP90 is conserved in each T. cacao gene. Cis-acting element analysis showed that TcHSP90 has important roles in growth and development as well as abiotic and hormonal stress responses including defense against pathogens. Phylogenetic analysis showed that TcHSP90 has a similar evolutionary distribution to A. thaliana and S. lycopersicum. The results of this study can be used as a scientific foundation and basic knowledge for the development of new T. cacao cultivars that are resistant to biotic and abiotic stresses by selecting suitable candidate genes for T. cacao breeding.
OPTIMIZATION OF PRIMARY SOMATIC EMBRYOGENESIS INDUCTION OFCOCOA (Theobroma cacaoL.) THROUGH THE ADDITION OF PHENOLICCOMPOUNDSUPPRESSANT MATERIALS Priandani, Putri Oktaviana; Pancaningtyas, Sulistyani; Ubaidillah, Mohammad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.1701

Abstract

Somatic embryogenesis is a propagation technique in which explants form somatic embryos. The percentage of primary somatic embryo formation in cocoa tends to be low due to the significant secretion of phenolic components in flower explants, thereby reducing the conversion rate of explants into embryos. This is indicated by browning that occurs in explants or the culture medium. The purpose of this study was to optimize primary somatic embryogenesis induction in cocoa through the addition of several types of phenolic compound suppressor materials to the culture medium. Staminodia explants were grownon DKW medium with factorial treatment: various phenolic compound suppressing materials (control; 300 mg L-1PVP; 15 mg L-1silver nitrate; 12 mg L-1melatonin; 300 mg L-1ascorbic acid) and cocoa cultivars (MCC-02 and ICCRI-09). The results showed that each material treatment produced a different response in suppressing phenolic compounds in each cultivar. The melatonin treatment resulted in the lowest percentage of browning intensity, which also had the best impact on morphology, callus size, wet weight,and the percentage of live explants.
Co-Authors Aisiyya Qutlana Munawaroh, Putri Ania Ningsih, Misbahul Helia Dewi, Nur Afni Mustafidah, Qori'atul Priandani, Putri Oktaviana Rahayu, Dwi Suci Su'udi, Mukhamad Su’udi, Mukhamad Tunjung Sari, Ariza Budi Ubaidillah, Mohammad