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All Journal Forum Pasca Sarjana Jurnal Kedokteran Hewan
Fachriyan H. Pasaribu
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Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Veterinary

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Isolation and Identification of Serum Gamma Immunoglobulin (IgG) of Native and Imported Chickens By Ion Exchange Chromatography and Immunochernistry Methods Murniaty Simorangkir; Aisjah Girindra; Fachriyan H. Pasaribu; Wasmen Manalu
Forum Pasca Sarjana Vol. 18 No. 1 (1995): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2381.821 KB)

Abstract

The study was designed to isolate and identify serum IgG of native and imported chickens, after being immunized with Newcastle Disease Vaccine. The isolation method used was the DEAE-Cellulose ion exchange chromatography using 0.01 M Tris-HCl buffer, at pH 8.0, with linear gradient NaCl from 0.01 M to 0.30 M after salting out with anhydrous Na2SO4. Identification of IgG characteristics carried out using the Ouchterlony double immunodiffusion, immuno-electrophoresis and SDS-PAGE 8% methods. Serum fractionation of native and imported chickens using the DEAE-Cellulose chromatography, after salting out with anhydrous Na2So4 of 18,14 and 14% resulted in four peaks of protein fractions.
Production of IgY Specific Antibody Against Entero Pathogenic Escherichia coli (EPEC) in Egg Yolk Teguh Wibawan, I Wayan; Pasaribu, Fachriyan H.; Rawendra, Rudi
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i1.9793

Abstract

Antibody to Enteropathogenic Eschericia coli (EPEC) K1.1. can be produced in egg yolk by the application of inactivated bacterial cells intravenously in layer chicken. The presence of specific antibody in sera and egg can bedetected with immunodiffusion techniques (Agar Gel Precipitation Test/AGPT), expressed by the occurrence of specific precipitation reaction between antibody and homolog antigen and no cross reaction of this antibody with antigen of Salmonella sp. and Klebsiella sp. The presence of specific antibody previously can be detected in sera, then 1 week after this the antibody start to be able be detected in egg yolk. The antibody is still present in sera as well as in egg yolk in large amount for 7 weeks and decrease significantly in the 8th week. This results indicated that theegg can be used in the producing specific antibody in the large quantity.
L3 Populations in Laying Hens Infected with 6,000 L2 of Ascaridia galli D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedono, Retno D.; Pasaribu, Fachriyan H.
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i2.3122

Abstract

The aim of the present study was to determine the survival of L3 populations in intestine ofchickens exposed to experimental Ascaridia galli infection. Nature female adult worm were obtained fromlumen of village chickens in a comercial abattoir in Bogor. The eggs (L1) obtained from uteri female adultworms were incubated in sterile aquadestilata at room temperature for 10-20 days developed embrionatedeggs (L2). Five groups (A-D) of 80 head chickens were infected with, 6000 L2 A. galli respectively. Thechickens of group A were infected six times with dose of each 1,000 L2 with an interval of one hour. Thechickens of group B were infected three times with dose of each 2,000 L2 with an interval of two hours.The chickens of group C were infected six times with dose of each 3,000 L2 with an interval of three hours. The chickens of group D were infected one time with single dose 6,000 L2. A. galli L3 were recovered from intestines of 80 heads chickens seven days after oesophagus inoculation with 6,000 L2.The result showed that total 702,000 L1 and 628,000 L2 collected from 124 A. galli female adult worms.The percentage of L1 developed L2 is 89.46% and L2 developed L3 is 11.27%. Significant survival of L3higher populations in intestine of chickens observed only in the group D. The results indicated thatchickens infected high dose of A. galli caused the decrease of host defence against ascaridiosis. Keywords: Ascaridia galli, embrionated eggs, larvae
Co-Authors Aisjah Girindra Darmawi D I wayan Teguh Wibawan Murniaty Simorangkir Rawendra, Rudi Retno D. Soejoedono Risa Tiuria Ummu Balqis Wasmen Manalu