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All Journal Jurnal Ilmu Kefarmasian Indonesia Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan CERATA
Herma Fanani Agusta
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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OPTIMASI PRODUKSI ANTIBAKTERI CAIRAN KULTUR ISOLAT BAKTERI (ISOLAT Te234) TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus Sandi Kurniawan; Alfian Syarifuddin; Herma Fanani Agusta; Missya Putri Kurnia Pradani
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 5 No 2 (2020): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.542 KB) | DOI: 10.36387/jiis.v5i2.449

Abstract

Antibiotics are metabolic products produced by certain microorganisms that can also damage other microorganisms. About 22,000 secondary metabolites in the form of antibiotics are produced by microbes, 70% are produced by actinomycetes, 20% by fungi, 7% Bacillus spp. and 1–2% by other bacteria. This research was conducted to determine the optimal time for bacteria to produce antibacterial which can inhibit the growth of Staphylococcus aureus and Escherichia coli. The method used is the well method with a diameter of 6.00 mm wellbore. The test results obtained, isolates of Te234 can produce optimal antibacterial which can inhibit the growth of Staphyloccus aureus bacteria on the 13th day with inhibition zone diameter of 16.00 ± 4.00mm against Escherichia coli bacteria on the 14th day with inhibitory zone diameter of 15, 33 ± 1.15mm so that it can be used as a determinant of the incubation time to produce antibacterial compounds. The TE 234 isolates are able to produce antibiotics that have the potential for the Staphylococcus aureus bacteria optimally on the 13th day, while on Escherichia coli on the 14th day
Penentuan Kurva Pertumbuhan dan Aktivitas Antibakteri dari Isolat Ekstrak Etil Asetat Bakteri (Te.325) terhadap Staphylococcus aureus dan Escherichia coli Alfian Syarifuddin; Ratna Wijayatri; Ichsan Feri Kurniawan; Herma Fanani Agusta
JURNAL ILMU KEFARMASIAN INDONESIA Vol 20 No 2 (2022): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v20i2.1079

Abstract

The development of infection cases and inappropriate use of antibiotics has led to cases of antibiotic resistance. An alternative to overcoming the many antibiotics that are already resistant to bacteria has led to the discovery of new antibiotics. One of the processes of discovering antibiotics is from microorganisms, namely bacteria. The exploration process for the discovery of antibiotics uses a bacterial growth phase approach, namely the stationary phase which produces secondary metabolites, one of, which are bacteria that contain antibiotic compounds. Te.325 isolate is a producer of bacterial antibiotics but The growth phase is not yet known and can be used to approach the process of obtaining antibiotics. The study was to obtain the growth phase time of the Te.325 isolate and to extract antibiotic compounds from the isolate. The determination of the growth curve is based on the weight of cell biomass and the absorbance value on UV/ Vis spectrophotometry of the culture sampled every day for 14 days of culture incubation. The results showed a log/exponential phase on 5th day and a stationary phase on 9th day. The activity test of the ethyl acetate extract was carried out using the well method with an extract concentration of 40%, which resulted in an average diameter of 8.04mm in Staphylococcus aureus and 9.035mm in Escherichia coli. The ethyl acetate extract of Te.325 has medium potency.
Standarisasi Parameter Spesifik Non Spesifik Ekstrak Etanol Daun Kersen (Muntingia calabura L) Asal Daerah Blitar Jawa Timur Jihan Arini; Ratna Wijayatri; Eka Sakti Wahyuningtyas; Estrin Handayani; Herma Fanani Agusta
CERATA Jurnal Ilmu Farmasi Vol 16 No 2 (2025): Cerata Jurnal Ilmu Farmasi
Publisher : Universitas Muhammadiyah Klaten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61902/cerata.v16i2.1642

Abstract

Muntingia calabura L, atau yang biasa disebut kersen banyak ditemukan di wilayah beriklim tropis Indonesia seperti daerah Blitar Jawa Timur. Tanaman kersen (Muntingia calabura L) banyak ditemukan pada wilayah beriklim tropis, serta temasuk dalam jenis tanaman perennial yang mampu tumbuh tinggi hingga 10 meter, dengan batang tegak yang berbentuk bulat, berkayu, dan memiliki sistem percabangan sympodial. Standarisasi tanaman kersen (Muntingia calabura L) memiliki tujuan sebagai upaya memperoleh produk herbal yang terjamin keamanan, khasiat, dan mutunya. Ekstrak tanaman ini distandarisasi berdasarkan dua parameter, yaitu parameter spesifik dan parameter non-spesifik. Parameter spesifik, tanaman yang digunakan adalah (Muntingia calabura L), dengan bagian yang dimanfaatkan yaitu daun (folium). Hasil uji organoleptik memperlihatkan bahwa ekstrak yang diperoleh memiliki konsistensi yang kental berwarna hijau tua kehitam-hitaman, beraroma khas pahit, serta memiliki rasa pahit. Pengujian kandungan kimia mengungkap bahwa ekstrak etanol daun kersen (Muntingia calabura L) memiliki kandungan alkaloid, flavonoid, saponin, tannin, dan terpenoid. Pada parameter non-spesifik, hasil uji kadar air menunjukkan bahwa ekstrak etanol daun kersen (Muntingia calabura L) memiliki kadar air 0%, dengan bobot jenis sebesar 1,0443 g/ml. Selain itu, hasil analisis juga menunjukkan bahwa ekstrak ini bebas dari cemaran logam berat, baik kadmium (Cd) maupun timbal (Pb).
Co-Authors Alfian Syarifuddin Eka Sakti Wahyuningtyas Estrin Handayani Ichsan Feri Kurniawan Jihan Arini Missya Putri Kurnia Pradani Ratna Wijayatri Sandi Kurniawan