Article Per Year (5 Year)
p-Index From 2020 - 2025
0.659
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Kefarmasian Indonesia Jurnal Ilmu Kesehatan Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan
Alfian Syarifuddin
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
OPTIMASI PRODUKSI ANTIBAKTERI CAIRAN KULTUR ISOLAT BAKTERI (ISOLAT Te234) TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus Sandi Kurniawan; Alfian Syarifuddin; Herma Fanani Agusta; Missya Putri Kurnia Pradani
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 5 No 2 (2020): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.542 KB) | DOI: 10.36387/jiis.v5i2.449

Abstract

Antibiotics are metabolic products produced by certain microorganisms that can also damage other microorganisms. About 22,000 secondary metabolites in the form of antibiotics are produced by microbes, 70% are produced by actinomycetes, 20% by fungi, 7% Bacillus spp. and 1–2% by other bacteria. This research was conducted to determine the optimal time for bacteria to produce antibacterial which can inhibit the growth of Staphylococcus aureus and Escherichia coli. The method used is the well method with a diameter of 6.00 mm wellbore. The test results obtained, isolates of Te234 can produce optimal antibacterial which can inhibit the growth of Staphyloccus aureus bacteria on the 13th day with inhibition zone diameter of 16.00 ± 4.00mm against Escherichia coli bacteria on the 14th day with inhibitory zone diameter of 15, 33 ± 1.15mm so that it can be used as a determinant of the incubation time to produce antibacterial compounds. The TE 234 isolates are able to produce antibiotics that have the potential for the Staphylococcus aureus bacteria optimally on the 13th day, while on Escherichia coli on the 14th day
Penentuan Kurva Pertumbuhan dan Aktivitas Antibakteri dari Isolat Ekstrak Etil Asetat Bakteri (Te.325) terhadap Staphylococcus aureus dan Escherichia coli Alfian Syarifuddin; Ratna Wijayatri; Ichsan Feri Kurniawan; Herma Fanani Agusta
JURNAL ILMU KEFARMASIAN INDONESIA Vol 20 No 2 (2022): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v20i2.1079

Abstract

The development of infection cases and inappropriate use of antibiotics has led to cases of antibiotic resistance. An alternative to overcoming the many antibiotics that are already resistant to bacteria has led to the discovery of new antibiotics. One of the processes of discovering antibiotics is from microorganisms, namely bacteria. The exploration process for the discovery of antibiotics uses a bacterial growth phase approach, namely the stationary phase which produces secondary metabolites, one of, which are bacteria that contain antibiotic compounds. Te.325 isolate is a producer of bacterial antibiotics but The growth phase is not yet known and can be used to approach the process of obtaining antibiotics. The study was to obtain the growth phase time of the Te.325 isolate and to extract antibiotic compounds from the isolate. The determination of the growth curve is based on the weight of cell biomass and the absorbance value on UV/ Vis spectrophotometry of the culture sampled every day for 14 days of culture incubation. The results showed a log/exponential phase on 5th day and a stationary phase on 9th day. The activity test of the ethyl acetate extract was carried out using the well method with an extract concentration of 40%, which resulted in an average diameter of 8.04mm in Staphylococcus aureus and 9.035mm in Escherichia coli. The ethyl acetate extract of Te.325 has medium potency.
Phytochemical Screening and Thin Layer Chromatography Analysis of Ethanol Extract of Bangle Rhizome (Zingiberis cassumunar Roxb.) Ananda Choirinisa Eka Putri; Perdana Priya Haresmita; Indra Yudhawan; Diyah Tri Utami; Alfian Syarifuddin
Jurnal Ilmu Kesehatan Vol 13 No 1 (2025): Jurnal Ilmu Kesehatan
Publisher : Universitas Muhammadiyah Kalimantan Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bangle rhizome (Zingiber cassumunar Roxb.) is a part of the plant that is widely used in traditional medicine because its diverse secondary metabolites have great potential in treating various diseases. The aim of this research is to identify the content of curcumin and active compounds found in bangle rhizome (Zingiber cassumunar Roxb.) qualitatively and quantitatively using Thin Layer Chromatography and UV-Vis Spectrophotometry methods. The maceration method was used to extract the bangle rhizome with 70% ethanol solvent, resulting in an extract yield of 7.92%. The results of the study from qualitative analysis using Thin Layer Chromatography (TLC) with 3 different mobile phases, namely chloroform: ethanol (95:5), chloroform: methanol: glacial acetic acid (94:5:1), and chloroform: n-hexane: methanol (1:1:0.1), indicate that the ethanol extract from the bangle rhizome positively contains polyphenolic compounds, terpenoids, and glycosides. Quantitative analysis of curcumin using UV-Vis Spectrophotometry at a wavelength of 437 nm resulted in a linear equation y = 0.026x + 0.1026 with an R² value of 0.9958 and r = 0.997898. The absorbance values from the 3 replicates of the bangle rhizome ethanol extract sample solution obtained in this study were 0.35 nm; 0.38 nm; 0.392 nm, indicating the presence of curcumin in all three replicates of the bangle rhizome ethanol extract solution. Based on the research results, it can be concluded that the ethanol extract of bangle rhizome (Zingiber cassumunar Roxb.) positively contains polyphenolic compounds, terpenoids, glycosides, and curcumin.
Co-Authors Ananda Choirinisa Eka Putri Diyah Tri Utami Herma Fanani Agusta Ichsan Feri Kurniawan Indra Yudhawan Missya Putri Kurnia Pradani Perdana Priya Haresmita Ratna Wijayatri Sandi Kurniawan