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All Journal JURNAL ENGGANO Jurnal Sumberdaya Akuatik Indopasifik Jurnal Perikanan Pantura
Abu Bakar Sambah
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IDENTIFIKASI MOLEKULER ISOLAT BAKTERI SELULOLITIK DARI MANGROVE SUNGAILIAT DAN TUKAK SADAI DI PULAU BANGKA Ardiansyah Kurniawan; Suci Puspita Sari; Euis Asriani; Andi Kurniawan; Abu Bakar Sambah; Asep Awaludin Prihanto
JURNAL ENGGANO Vol 3, No 2
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.351 KB) | DOI: 10.31186/jenggano.3.2.250-260

Abstract

Bakteri selulolitik memiliki kemampuan degradasi selulosa dan membuat karbohidrat lebih mudah dicerna bagi ternak. Penelitian ini bertujuan untuk mengidentifikasi bakteri selulolitik di tanah, kayu lapuk dan daun dari mangrove pulau Bangka melalui skrining dan analisis gen 16s rRNA. Penelitian dilakukan dari September 2017 hingga Maret 2018. Isolat TSL7 dan TSS4 dari Mangrove Tukak Sadai dan SLS5 dari Mangrove Sungailiat yang memiliki kemampuan mendegradasi selulosa terbesar berdasarkan hasil skrining menjadi isolat yang diidentifikasi pada gen 16S rRNA untuk diurutkan dan analisis BLAST. Analisis gen bank menunjukkan kedekatan dengan Pseudomonas aeruginosa dengan query cover 37% - 87%. Hasil query cover rendah dihasilkan karena sekuens yang sesuai pendek. Kontaminasi dan perubahan komunitas karena seleksi lingkungan dengan adanya logam berat dapat menjadi penyebab dominasi Pseudomonas aeruginosa. Pseudomonas aeruginosa yang bersifat patogen tidak disarankan untuk diaplikasikan sebagai pendegradasi selulosa pakan ternak. 
KARAKTERISASI BIOKIMIA BAKTERI SELULOLITIK DARI KAYU LAPUK MANGROVE DI SUNGAILIAT DAN TUKAK SADAI, PULAU BANGKA Ardiansyah Kurniawan; Asep Awaludin Prihanto; Suci Puspita Sari; Andi Kurniawan; Euis Asriani; Abu Bakar Sambah
Jurnal Sumberdaya Akuatik Indopasifik Vol 2 No 1 (2018): Mei
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (223.639 KB) | DOI: 10.30862/jsai-fpik-unipa.2018.Vol.2.No.1.50

Abstract

Acceleration of wood weathering in the mangrove area is possible with the role of cellulolytic bacteria. Cellulolytic bacteria producing cellulase enzymes are needed by the animal feed and agriculture industries. Mangroves in Muntok Subdistrict undergo a process of adaptation to environmental changes due to tin mining, including microorganisms. Exploration of new species and strains of cellulolytic bacteria in miningaffected mangroves is needed to enrich the collection and its potential use for human needs. This study aims to identify cellulolytic bacteria in mangrove weathered wood in Muntok District, West Bangka Regency through isolation, screening, and biochemical characterization. Three sampling locations, namely Sukal Mangrove, Peltim Mangrove, and Tembelok Mangrove result 22 bacterial isolates with 11 isolates showing the ability of cellulose degradation in qualitative tests using Lugol. The biochemical characterization of bacterial isolates with the greatest cellulose degradation was directed towards Citrobacter freundii and Vibrio alginolyticus in samples of Sukal Mangrove and Actinomyces bovis in isolates from Peltim Mangrove.
ISOLASI DAN IDENTIFIKASI BAKTERI PENDEGRADASI SELULOSA ASAL EKOSISTEM MANGROVE TUKAK SADAI, BANGKA SELATAN Ardiansyah Kurniawan; Dwi Febrianti; Suci Puspita Sari; Asep Awaludin Prihanto; Euis Asriani; Andi Kurniawan; Abu Bakar Sambah
Jurnal Perikanan Pantura (JPP) Vol 1 No 2 (2018): SEPTEMBER 2018
Publisher : Universitas Muhammadiyah Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.814 KB) | DOI: 10.30587/jpp.v1i2.461

Abstract

Perikanan dan Kelautan, Universitas Brawijaya3Dosen Fakultas Perikanan dan Ilmu Kelautan, Universitas Brawijaya.ABSTRACTAgricultural waste has problems on fiber and cellulose digestibility in its utilization foraquaculture. The ability of plant cellulose degrading bacteria to become a source ofenergy can increase the digestibility of feed by fish. The purpose of this study was toidentify the cellulolytic bacteria from the mangrove ecosystem, Tukak Sadai District,South Bangka Regency. This study was conducted in March until August 2017.Samples were taken from litter, mud and weathered wood and isolated usingCarboxymetyl Cellulosa (CMC) 1% media and found 22 bacterial isolates. Gramstaining results showed that 3 isolates (TSS 2, TSL 6 and TSK 4) were classified asgram positive and 19 other isolates were gram negative. Cellulolytic test results showed6 isolates had the ability to degrade cellulase, namely 3 isolates from mangrove mudsamples (TSS 1, TSL 7, TSL 1), 2 isolates from leaf litter (TSS 4, TSL 2) and 1 isolatefrom weathered wood (TSK 5) . Sequencing of 16S rRNA gene DNA showed proximityto Pseudomonas aeruginosa in TSL 7 and TSS isolates 4
Co-Authors Andi Kurniawan Andi Kurniawan Ardiansyah Kurniawan Asep Awaludin Prihanto Dwi Febrianti Euis Asriani Euis Asriani Euis Asriani Suci Puspita Sari Suci Puspita Sari Suci Puspita Sari