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Effect of Fish Transportation on immunological response and Bacterial Diseases in Anabas Testudineus Fish Hardi, Esti Handayani; Asmir, Asmir; Pebrianto, Catur Agus; Saptiani, Gina; Agustina, Agustina
Aquacultura Indonesiana Vol 16, No 2 (2015): Volume 16 Issue 2 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.795 KB) | DOI: 10.21534/ai.v16i2.11

Abstract

This study was conducted to determine the condition of immunological response of and the presence of bacterial diseases in Anabas Testudineus fish was transported from pond culture towards Segiri Market. Transport system carried out by the fishmonger supposed to influence the health conditions of fish as well as the presence of bacteria on the fish's body. The sample was 30 fish from Muara Kaman pond and Markets Segiri as a fish marketing. Some parameters were observed among others: changes in swimming behavior, pathology anatomy organs, aemotology and inventory of bacterial diseases. The results showed that the samples of fish from the pond aquaculture generally have normal conditions of organ anatomy, symptom behavior swim also normally, otherwise fish from marketed in the Market Segiri, as much as 60% of fish have abnormal behavior of swimming, 73% have pathology on external anatomy and 53% fish have abnormalities in organs. Results of the isolation and identification of bacterial disease found that there are 5 types of bacteria Aeromonas, Pseudomonas, Staphyllococcus, Streptococcus and Basillus, and only bacteria Staphyllococcus and Basillus not found in fish pond aquaculture origin. The health condition of the fish pond aquaculture origin betook better, judging from the hemoglobin, hematocrit, total leukocyte and total erythrocyte that is within the normal range. While the blood picture fish from Segiri market is generally higher in all parameters that indicate health problems. It can be concluded that the transport system carried out by the wet etching fishmonger effect on the occurrence of abnormalities in behavioral symptoms, anatomic pathology outer and inner organs, the presence of bacterial disease and the decline in fish health conditions.Keywords : Betok fish, bacterial, transportation
Ekstrak Daun Pepaya dan Kangkung untuk Meningkatkan Daya Tetas Telur dan Kelangsungan Hidup Larva Lele (EXTRACTS OF CARICA PAPAYA AND IPOMOEA AQUATICA FOR IMPROVING EGG HATCHABILITY AND LARVAL VIABILITY OF CATFISH) Gina Saptiani; Esti Handayani Hardi; Catur Agus Pebrianto; Agustina Agustina
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.322 KB)

Abstract

This research was aimed to investigate the potential use of leaf extract of Carica papaya and Ipomoeaaquatica lto improve egg hatchability and larval viability of catfish. Dried leaves of Carica papaya andIpomoea aquatica were macerated and extracted in water and ethanol. Eggs and larvae were tested in theaquarium size of 10 L with a a diameter of 28 cm. The extracts in concentration 600, 800 and 1.000 ppmwere tested on the egg hatchability of catfish with immersion method, and challed with Aeromonashydrophyla, Pseudomonas sp., and Saprolegnia spp. The extracts in concentration 800 and 1.000 ppm weretested on the larval viability with immersion method, and challed with pathogens. Water or ethanolextract of Carica papaya and Ipomoea aquatica can improve egg hatchability 67±8% until 90±6% andlarval viability of catfish 77±0,5 until 90±9%. Eight hundred ppm ethanol extract of Carica papaya has thebest egg hatchability and 1000 ppm can improve larval viability of catfish.
Toksisitas Produk Ekstraseluler dan Intraseluler Bakteri Pseudomonas sp. pada Ikan Nila (Oreochromis niloticus) (TOXICITY OF EXTRACELLULAR AND INTRACELLULAR PRODUCT OF PSEUDOMONAS SP IN TILAPIA (OREOCHROMIS NILOTICUS) Esti Handayani Hardi; Catur Agus Pebrianto; Gina Saptiani
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.236 KB)

Abstract

The aim of the research was to investigate the toxicity of extracellular product (ECP) and intracellularproduct (ICP) of Pseudomonas sp. on tilapia. A total of 40 tilapias weighing 15 grams were injected withECP and ICP. The ECP and ICP were harvested from Pseudomonas sp. culture on two kinds of culturemedia and different time of incubation. The Pseudomonas was cultured on trypticase soy agar (TSA) andtrypticase soy broth (TSB) and incubated at 24, 48 and 72 hours. The slurry of the bacteria was centrifugedat 10000 g, for 30 minutes on 4oC to get ECP and in room temperature to get ICP. The supernatant wasfiltered with 0.45 ?m paper mesh. A hundred percent mortality was found in tilapia six hours postinjection with ICP (72 hours) whereas tilapias were injected with ECP caused 60% mortality in 12 hours.The tilapia showed whirling at 24 hour post injected with ECP of Pseudomonas sp which was cultured inTSA for 48 hours incubated. Opacity of the cornea and exopthalmia were occurred at 48 hours postinjection of ECP and ICP which were harvested from both media. Injection of ICP caused pathologychanges on internal organ of fish i.e. pale appearance of spleen and liver. In conclusion, the ECP and ICPwere a virulence factors of Pseudomonas sp. and the ICP seem more pathogenic and caused mortality thanECP. Both culture media and time of incubation influence of ECP and ICP production. The ECP and ICPwhich were harvested from Pseudomonas sp incubate for 24-48 hour more virulent than 72 hour.
Co-Authors Asmir, Asmir Esti Handayani Hardi Gina Saptiani Gina Saptiani Gina Saptiani Ida Ayu Putu Sri Widnyani