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All Journal Jurnal Akuakultur Indonesia Jurnal Akuakultur Rawa Indonesia Jurnal Natur Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Bioeksperimen: Jurnal Penelitian Biologi JURNAL GALUNG TROPIKA Jurnal Perikanan Universitas Gadjah Mada Jurnal Akuakultur Sungai dan Danau Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Seminar Nasional Lahan Suboptimal Aquacultura Indonesiana ABDINE Jurnal Pengabdian Masyarakat CONSEN: Indonesian Journal of Community Services and Engagement Journal of Research Applications in Community Services Ilmu Perikanan Tropis Nusantara Jurnal Kedokteran Hewan ANDIL Mulawarman Journal of Community Engagement ABDIKU : Jurnal Pengabdian Masyarakat Universitas Mulawarman

Esti Handayani Hardi

Mulawarman University

Author-ID : 273990

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Effect of Fish Transportation on immunological response and Bacterial Diseases in Anabas Testudineus Fish Hardi, Esti Handayani; Asmir, Asmir; Pebrianto, Catur Agus; Saptiani, Gina; Agustina, Agustina
Aquacultura Indonesiana Vol 16, No 2 (2015): Volume 16 Issue 2 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

This study was conducted to determine the condition of immunological response of and the presence of bacterial diseases in Anabas Testudineus fish was transported from pond culture towards Segiri Market. Transport system carried out by the fishmonger supposed to influence the health conditions of fish as well as the presence of bacteria on the fish's body. The sample was 30 fish from Muara Kaman pond and Markets Segiri as a fish marketing. Some parameters were observed among others: changes in swimming behavior, pathology anatomy organs, aemotology and inventory of bacterial diseases. The results showed that the samples of fish from the pond aquaculture generally have normal conditions of organ anatomy, symptom behavior swim also normally, otherwise fish from marketed in the Market Segiri, as much as 60% of fish have abnormal behavior of swimming, 73% have pathology on external anatomy and 53% fish have abnormalities in organs. Results of the isolation and identification of bacterial disease found that there are 5 types of bacteria Aeromonas, Pseudomonas, Staphyllococcus, Streptococcus and Basillus, and only bacteria Staphyllococcus and Basillus not found in fish pond aquaculture origin. The health condition of the fish pond aquaculture origin betook better, judging from the hemoglobin, hematocrit, total leukocyte and total erythrocyte that is within the normal range. While the blood picture fish from Segiri market is generally higher in all parameters that indicate health problems. It can be concluded that the transport system carried out by the wet etching fishmonger effect on the occurrence of abnormalities in behavioral symptoms, anatomic pathology outer and inner organs, the presence of bacterial disease and the decline in fish health conditions.Keywords : Betok fish, bacterial, transportation

Immunomodulatory effect and Disease resistance from concoction three of Borneo plant extracts in tilapia, Oreochromis niloticus kusuma, W; Apriza, Apriza; hardi, esti handayani; Nugroho, RA; Kusuma, I W
Aquacultura Indonesiana Vol 20, No 1 (2019)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21534/ai.v20i1.135

This study was conducted to evaluate the immunomodulatory activity and diseases resistance of concoction Boesenbergia pandurata/BP, Solanum ferox/SF, and Zingiber zerumbet/ZZ extract (SF, BP, and ZZ) in tilapia with the concentrations 600 mg/L BP, 900 mg/L SF and 200 mg/L ZZ against Aeromonas hydrophila and Pseudomonas fluorescens. Immunomodulatory activity was measured by testing the concoction of three plants extract to preventive and treatment pathogen infection (A. hydrophila and P. fluorescens 105colony-forming units per milliliter each bacteria). In this research, using feed administration method, the concoction of extract BP:SF:ZZ ratio was 1:1:1 and extract is given for 14 days as much as three times a day (3-5% of fish body weight). The immunomodulatory parameters (white blood cell/WBC, red blood cell/RBC, phagocytic index/IP) and relative presentation survival/RPS was observed at week 4th after challenges with bacteria through intramuscularly injection (0.1 mL/fish). The number of white blood cell (WBC) was increased significantly (P <0.05) compared to controls without extract and it also happens with red blood cell (RBC), phagocytic index also experienced a significant increasing in fish fed with combined extracts compared to controls. The results of this study indicate that giving concoction of three extracts provides the best protection (RPS) against infection of A. hydrophila and P. fluorescent. The conclusion this research is concoction of SF:BP:ZZ in the ratio of 1:1:1 have a immunomodulatory effect in tilapia and increasing protection against bacterial infections

Toksisitas Produk Ekstrasellular (ECP) Streptococcus agalactiae pada Ikan Nila (Oreochromis niloticus) Hardi, Esti Handayani; Sukenda, Sukenda; Harris, Enang; Lusiastuti, Angela Mariana
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

This research aimed to know the toxicity of extracellular products (ECP) of Streptococcus agalactiae was tastedin cultured Nile tilapia (Oreochromis niloticus). Streptococcus agalactiae had two haemolytic types: β-haemolyticand non-haemolytic type. Toxicity test of ECP to know the virulancy factor of S. agalactiae was still limited. It wasfound that after tested on 15 fish weighing 15 g through intraperitoneal injection 0,1 ml/fish, both bacteria causedchanges in swimming pattern, palatability, external and internal anatomy macroscopically and microscopically.Extracellular products of S. agalactiae non-haemolytic type (BHIA and BHI 24 h) and β-haemolytic type (BHI 72 h)caused mortality 12 hours after injection and the mortality continued till day 7 th of culture. Whirling happened 96hours after injection with ECP S. agalactiae β-haemolytic type (BHIA 72 h incubation) whereas injection with ECP(BHI 24 h) on 72 h after injection and continued untill day 7 th. Behavior disease signs caused by S. agalactiaeoccured on eyes. There were opacity, purulens, eye shrink, lateral and bilateral exopthalmia and haemorrhage oninfected-fish. Silver staining of sodium dodecyl sulphate-polyacrylamide gels to S. agalactiae revealed thatpredominant 51.8-69.6 kDa bands were present in BHIA ECP fraction. The 69.6 kDa was absent from the BHI ECP.Total protein on non-haemolytic S. agalactiae ECP are 28.18 ppm on BHIA medium and 13.64 ppm on BHI medium.Whereas β-haemolytic S. agalactiae ECP are 2.73 ppm on BHIA medium and 8.18 ppm on BHI medium. Concentrationof protein in ECP was one of factor that caused non-haemolytic S. agalactiae more virulent than β-haemolytic type.The conclusion from the research that ECP was virulent factor on β-haemolytic and non-haemolytic S. agalactiaein fish which caused changes in behavior disease signs.

PENGARUH PENAMBAHAN FITOIMUN® KE DALAM PAKAN TERHADAP PERTUMBUHAN KEPITING BAKAU (Scylla serrata) YANG DIPELIHARA DI TAMBAK SILVOFISHERY DESA SALO PALAI KECAMATAN MUARA BADAK KABUPATEN KUTAI KARTANEGARA Wanda Rachmawati; Gina Septiani; Esti Handayani Hardi
Jurnal Akuakultur Rawa Indonesia VOL 10, NO 2 (2022): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/jari.v10i2.19268

Mangrove crab (Scylla serrata) is a crab that has high potential and is cultivated in mangrove areas, so that many people keep mangrove crabs. However, there are still many obstacles, including slow growth. The purpose of this study was to examine the use of Fitoimun® mixed into crab feed to see survival, weight growth, specific daily growth rate, feed conversion ratio, and carapace width, which were reared in silvofishery ponds in Salo Palai Village. The study used a completely randomized design with 3 treatments and 3 replications. The treatment was Fitoimun® products containing Solanum ferox L and Boesenbergia pandurata, with a dose of 0 mL/kg, 20 mL/kg, 24 mL/kg. This study used mud crabs a weight of about 187-300 grams and a carapace width of about 95.0-110.5 mm. Feeding is given once every 2 days, in the morning at 07.00-08.00 and in the afternoon at 16.00-17.00. The feed given to crabs was measured as 5% of the crab's biomass weight. The results showed that the use of Fitoimun® products had no significant effect on survival, absolute weight growth, specific daily growth rate, carapace width, and feed conversion ratio, water quality during the study temperature 28.5°C, pH 7.5, DO 5.8 mg/L, salinity 13.8 mg/L pond. The relatively good Fitoimun® at a dose of 20 mL/kg mixed into mud crab (Scylla serrata) feed was able to increase growth, specific daily growth rate, feed conversion ratio, and carapace width. Keywords: Ftoimun®, growth survival, mud crab.

Efek penginjeksian produk intraseluler (icp) dan ekstraseluler (ecp) bakteri Pseudomonas sp. terhadap gambaran darah ikan nila (Oreochromis niloticus): Effect of ECP and ICP Pseudomonas sp. injected to haematology of nile tilapia Dosim; Esti Handayani Hardi; Agustina
Jurnal Ilmu Perikanan Tropis Nusantara (Nusantara Tropical Fisheries Science Journal) Vol. 1 No. 2 (2022): Ilmu Perikanan Tropis Nusantara
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jipt.v1i2.430

This study aimed to determine the effect of Pseudomonas ECP and ICP injection incubated on TSA and TSB media with incubation periad of 72 hours on the nila tilapia (O. niloticus) haematology. Parameters observed were hemoglobin, hematocrit, erythrocyte total and of leukocyte total. The ECP or ICP ware injected into intraperitial nila tilapia as much as 0.2 ml/fish. Observations were made on 6, 12, 24, 48, 72, 96, 120, 144, 168 hour after injection. Extracellular products were obtained by centifuging Pseudomonas suspension grown on TSA and TSB media (incubation time 72 h + 30oC) using a 10.000 g centrifuge for 30 min at 4oC. Supernatant was filtered using filter paper 0.45 μm. To get the ICP, supernatant was centrifuged in room temperature. The results revealed that ICP injection caused the decrease of haematology faster than ECP injection. This indicates that the ICP Pseudomonas more virulent than ECP.

Pertumbuhan dan percepatan molting kepiting bakau (Scylla serrata) yang diberi ekstrak temu kunci (Boesenbergia pandurata) 3 In 1 BIOIMUN® di tambak silvofishery Desa Salo Palai Kecamatan Muara Badak Kabupaten Kutai Kartanegara: The growth and accelerate molt of mud crab (Scylla serrata) given Boesenbergia pandurata extract in silvofishery ponds Salo Palai Village Muara Badak District Kutai Karanegara Regency Andre Jolpano; Esti Handayani; Gina Saptiani
Jurnal Ilmu Perikanan Tropis Nusantara (Nusantara Tropical Fisheries Science Journal) Vol. 2 No. 1 (2023): Ilmu Perikanan Tropis Nusantara
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jipt.v2i1.76

This research aimed to study the administrasion of B. pandurata extract mixed with crab feed on the percentage of molting, growth, and survival, and feed conversion ratio of mud crabs reared in silvofishery ponds. This research was conducted in Salo Palai Village, Muara Badak District, Kutai Kartanegara Regency. This study used a completely randomized design, with 4 treatments and 3 replications. The extract of B. pandurata in the form 3 In BIOIMUN® drug there are 4 treatment dose dasnamely (0 mL/kg, 20 mL/kg, 25 mL/kg, 30 mL/kg feed). The crabs used came from the catch of the Muara Badak community with a size of 200-280 g, and were culture for 4 weeks (30 days). The crabs were fed fresh fish with the addition of extract 2 times a day, in the morning (07.00-08.00) and afternoon (16.00-17.00), but feeding was done every 2 days. The feed given was weighed as much as 5% of the weight of the crab. The results showed that the administration of 3 In 1 BIOIMUN® in treatment P1 (20 mL/kg) affect the percentage of molting, growth, survival, and feed conversion ratio.

INFEKSI Aeromonas hydrophila MELALUI JALUR YANG BERBEDA PADA IKAN NILA (Oreochromis niloticus) DI LOA KULU KUTAI KARTANEGARA KALIMANTAN TIMUR Esti Handayani Hardi; Catur Agus Pebrianto; Triesna Hidayanti; Rizki Tri Handayani
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Tujuan penelitian ini adalah mengetahui patogenisitas bakteri Aeromonas hydrophila (A. hydrophila) yang diinfeksi dengan jalur yang berbeda yaitu melalui perendaman, pakan, injeksi intraperitoneum dan injeksi intramuskular. Kepadatan bakteri 10 10 cfu/ml bakteri A. hydrophila diinfeksikan pada ikan nila berukuran 15 g melalui empat jalur infeksi yang berbeda. Dari hasil pengamatan diketahui bahwa in feksi melalui perendaman, pakan, injeksi intraperitoneum, dan injeksi intramuskular merupakan port entry atau jalan masuk bakteri A. hydrophila terhadap ikan nila yang bermakna bakteri mampu menyebarkan virulensi (menyebabkan ikan sakit dan atau mati) melalui air (media hidup), saluran pencernaan melalui rongga perut, dan melalui pembuluh darah. Kondisi ini dapat dilihat dari perubahan pada pola renang, penurunan nafsu makan, patologi anatomis organ dalam dan luar serta perubahan gambaran darah. Infeksi bakteri A. hydrophila melalui penyuntikan merupakan jalur infeksi yang menyebabkan ikan nila mengalami kematian lebih cepat dan lebih banyak dibandingkan dengan jalur infeksi melalui pakan dan perendaman. Namun injeksi melalui muskular merupakan jalur infeksi yang menyebabkan kematian lebih cepat. Infeksi melalui injeksi merupakan infeksi yang juga menyebabkan perubahan pada pola renang, patologi anatomi lebih cepat dibandingkan dengan jalur infek si yang lain.

Strengthening Legal Capacity of Micro, Small, and Medium Enterprises in the Mangrove Ecosystem of Mahakam Delta: Penguatan Kapasitas Hukum Pelaku Usaha Mikro Kecil dan Menengah di Kawasan Ekosistem Mangrove Delta Mahakam Haris Retno Susmiyati; Grizelda Grizelda; Rahmawati Al Hidayah; Warkhatun Najidah; Wiwik Harjanti; Alfian; Esti Handayani Hardi; Rita Diana; Nurul Puspita Palupi
CONSEN: Indonesian Journal of Community Services and Engagement Vol. 3 No. 1 (2023): Consen: Indonesian Journal of Community Services and Engagement
Publisher : Institut Riset dan Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

The economic independence of the community through Micro, Small, and Medium Enterprises (UMKM) is an important priority that supports social economy. Especially during a crisis and pandemic COVID19, UMKM are one of the business actors who are still able to survive when economic activity declines. However, in this great potential, UMKM actors in the mangrove ecosystem in the Mahakam delta area have weaknesses in legal understanding as evidenced by the incomplete licensing requirements, and also do not have an understanding of the urgency and procedures for making contracts and other legal aspects. Therefore, training is needed to increase the legal capacity of UMKM actors. The method used is a training involving UMKM business actors in three stages; UMKM mapping; provide material on opportunities and challenges for UMKM in mangrove ecosystems and legal knowledge for UMKM; preparation of strategic plans for UMKM for mangrove ecosystems. The results of this activity indicate an increase in the legal understanding of UMKM actors and a strategic plan for strengthening UMKM in the mangrove ecosystem in the Delta Mahakam area has been formulated. This encourages the community to participate in protecting and rehabilitating mangrove areas for mutual prosperity. Keywords: Micro, Small and Medium Enterprises ; Mangrove ; Delta Mahakam

Pelatihan Penyusunan Produk Hukum Desa Peduli Mangrove di Kawasan Delta Mahakam Dengan Metode ROCCIPI Haris Retno Susmiyati; Wiwik Harjanti; Rahmawati Al-Hidayah; Alfian; Esti Handayani Hardi
ABDINE: Jurnal Pengabdian Masyarakat Vol. 3 No. 1 (2023): ABDINE : Jurnal Pengabdian Masyarakat
Publisher : Sekolah Tinggi Teknologi Dumai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52072/abdine.v3i1.478

Mangrove merupakan kawasan penting bagi kehidupan masyarakat pesisir dan keberlangsungan layanan alam. Namun kondisi ekosistem mangrove di Kawasan Delta Mahakam masih mengalami kerusakan meskipun upaya rehabilitasi telah banyak dilakukan. Salah satu kelemahannya adalah belum adanya payung hukum terkait perlindungan dan rehabilitasi mangrove. Sehingga dibutuhkan upaya hukum berupa penyusunan produk hukum lokal terkait perlindungan dan rehabilitasi mangrove berbasis masyarakat. Pelatihan ini dilakukan melalui dua tahapan, antara lain: pemetaan produk hukum desa, dan penyusunan dengan metode Roccipi. Pemetaan di lima desa menunjukkan belum memiliki produk hukum yang berkaitan dengan rehabilitasi mangrove. Pelatihan produk hukum desa peduli mangrove dengan menggunakan metode ROCCIPI membentuk kesadaran hukum yang dibuktikan dengan keterlibatan seluruh pihak dalam menyusun kanvas desain peraturan desa peduli mangrove di lima desa yang berada di Kawasan Delta Mahakam. Hasil pemetaan dan pelatihan diperoleh data potensi penguatan produk hukum desa dan peningkatan kapasitas aparatur desa dan masyarakat dalam menyusun produk hukum desa peduli mangrove.

Pengaruh Penambahan Bungkil Inti Sawit Fermentasi Dengan Lama Waktu Berbeda Terhadap Konversi Pakan dan Pertumbuhan Ikan Kelabau (Osteochilus melanopleurus) Maria Damanik; Heru Kusdianto; Esti Handayani Hardi; Henny Pagoray; Isriansyah Isriansyah
Jurnal Akuakultur Sungai dan Danau Vol 8, No 2 (2023): Oktober
Publisher : Universitas Batangahari Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33087/akuakultur.v8i2.171

Kelabau (Osteochilus melanopleurus) is one of the local fish found in Borneo. Economically, kelabau fish has a reason to be cultivated because it has a fairly high price. One of the aspects that needs to be considered is the availability of raw materials. This study aimed to evaluate the use of fermented palm kernel meal in feed on feed conversion, growth, and survival of kelabau. The experimental design applied was a Completely randomized design (CRD) with 4 treatments and 3 replicates, namely (P0) no palm kernel meal added; (P2) palm kernel meal fermented for 0 hours, (P3) palm kernel meal fermentaded for 24 hours, and (P4) palm kernel meal fermented for 48 hours. Fish rearing was carried out for 40 days with feeding experimental frequency three times a day with at satiation. The fish was kelabau seeds measuring 4-5 cm and weighing 1-2 g. Ovserved variades tested in this study included feed conversion rasio , growth, and survival rate. The highest growth was resulted by palm kernel meal fermented for 48 hours, the lowest feed conversion ratio (FCR) was resulted by falm kernel meal fermented 24 hours, and the survival of all treatment during this rearing werw 100%. However statisticaly no significant differences were observed in either/ grow or feed conversion ratio. Kata kunci: Kelabau; EM4; fermentasi; bungkil inti sawit

Co-Authors , Agustina Agriandini, Maulina Agustina Al Hidayah, Rahmawati Alfian Alfian ALIM ISNANSETYO Amalia, Rizqi Andi Noor Asikin Andre Jolpano ANGELA MARIANA LUSIASTUTI Angela Mariana Lusiastuti Anjani, Ryan Apriza Apriza, Apriza Ardana Kurniaji Aryani, Retno Asih, Supadmi Asmir, Asmir Aziza, Aziza Banin, Maghfirotin Marta Catur Agus Pebrianto Catur Agus Pebrianto Catur Agus Pebrianto, Catur Diana, Rita Dosim Enang Harris Enang Harris Fikri Ardhani Fitriyana Fitriyana, Fitriyana Gina Saptiani Gina Saptiani Gina Saptiani Gina Saptiani Gina Septiani Grizelda Grizelda Halim, Aldi M. Haris Retno Susmiati Haris Retno Susmiyati Henny Pagoray Henny Pagoray, Henny Heru Kusdianto Hetty Manurung Hetty Manurung, Hetty Ida Ayu Putu Sri Widnyani Irawan Wijaya Kusuma, Irawan Wijaya Isriansyah Isriansyah Jolpano, Andre Komsanah Sukarti Komsanah Sukarti Krishna Purnawan Candra Kusuma, I W kusuma, W Lina Warlina Maria Damanik Marwati Marwati Maulida Rachmawati Maulida Rachmawati Maulina Agriandini Maulina Anggridini Mira Mawardi Naim, Muhammad Najli, Najli Niklani, Andi Nugroho, RA Nugroho, Rudi Agung Nurkadina Nurkadina Nurul Puspita Palupi Nurul Puspita Palupi Panggulu Ahmad Ramadhani Utoro Rahayu, Widianingsih Rahmah, Nur Rini Renitasari, Diana Putri Retno Aryani Reynata, Ricko Rita Diana Rita Diana Rizki Tri Handayani Rizki Tri Handayani, Rizki Rudianto Rudianto Rudianto Rudianto Rudy Agung Nugroho Sukenda . Sukenda . Supryady, Supryady Suwinarti, Wiwin Triesna Hidayanti Triesna Hidayanti, Triesna Wanda Rachmawati Warkhatun Najidah Wendrika, Wendrika Widyaningsih Rahayu Wiwik Harjanti Yulian Andriyani Yulian Andriyani, Yulian Yunarty Yunarty, Yunarty Yushinta Fujaya Zaidan Fadhilah, Raviv

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