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All Journal Jurnal Akuakultur Indonesia Jurnal Akuakultur Rawa Indonesia Jurnal Veteriner Jurnal Natur Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Bioeksperimen: Jurnal Penelitian Biologi JURNAL GALUNG TROPIKA Jurnal Perikanan Universitas Gadjah Mada Jurnal Akuakultur Sungai dan Danau Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Seminar Nasional Lahan Suboptimal Aquacultura Indonesiana ABDINE Jurnal Pengabdian Masyarakat CONSEN: Indonesian Journal of Community Services and Engagement Journal of Research Applications in Community Services Ilmu Perikanan Tropis Nusantara Jurnal Kedokteran Hewan ANDIL Mulawarman Journal of Community Engagement ABDIKU : Jurnal Pengabdian Masyarakat Universitas Mulawarman

Esti Handayani Hardi

Mulawarman University

Author-ID : 273990

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Effect of Fish Transportation on immunological response and Bacterial Diseases in Anabas Testudineus Fish Hardi, Esti Handayani; Asmir, Asmir; Pebrianto, Catur Agus; Saptiani, Gina; Agustina, Agustina
Aquacultura Indonesiana Vol 16, No 2 (2015): Volume 16 Issue 2 Year 2015
Publisher : Indonesian Aquaculture Society (MAI)

This study was conducted to determine the condition of immunological response of and the presence of bacterial diseases in Anabas Testudineus fish was transported from pond culture towards Segiri Market. Transport system carried out by the fishmonger supposed to influence the health conditions of fish as well as the presence of bacteria on the fish's body. The sample was 30 fish from Muara Kaman pond and Markets Segiri as a fish marketing. Some parameters were observed among others: changes in swimming behavior, pathology anatomy organs, aemotology and inventory of bacterial diseases. The results showed that the samples of fish from the pond aquaculture generally have normal conditions of organ anatomy, symptom behavior swim also normally, otherwise fish from marketed in the Market Segiri, as much as 60% of fish have abnormal behavior of swimming, 73% have pathology on external anatomy and 53% fish have abnormalities in organs. Results of the isolation and identification of bacterial disease found that there are 5 types of bacteria Aeromonas, Pseudomonas, Staphyllococcus, Streptococcus and Basillus, and only bacteria Staphyllococcus and Basillus not found in fish pond aquaculture origin. The health condition of the fish pond aquaculture origin betook better, judging from the hemoglobin, hematocrit, total leukocyte and total erythrocyte that is within the normal range. While the blood picture fish from Segiri market is generally higher in all parameters that indicate health problems. It can be concluded that the transport system carried out by the wet etching fishmonger effect on the occurrence of abnormalities in behavioral symptoms, anatomic pathology outer and inner organs, the presence of bacterial disease and the decline in fish health conditions.Keywords : Betok fish, bacterial, transportation

Immunomodulatory effect and Disease resistance from concoction three of Borneo plant extracts in tilapia, Oreochromis niloticus kusuma, W; Apriza, Apriza; hardi, esti handayani; Nugroho, RA; Kusuma, I W
Aquacultura Indonesiana Vol 20, No 1 (2019)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21534/ai.v20i1.135

This study was conducted to evaluate the immunomodulatory activity and diseases resistance of concoction Boesenbergia pandurata/BP, Solanum ferox/SF, and Zingiber zerumbet/ZZ extract (SF, BP, and ZZ) in tilapia with the concentrations 600 mg/L BP, 900 mg/L SF and 200 mg/L ZZ against Aeromonas hydrophila and Pseudomonas fluorescens. Immunomodulatory activity was measured by testing the concoction of three plants extract to preventive and treatment pathogen infection (A. hydrophila and P. fluorescens 105colony-forming units per milliliter each bacteria). In this research, using feed administration method, the concoction of extract BP:SF:ZZ ratio was 1:1:1 and extract is given for 14 days as much as three times a day (3-5% of fish body weight). The immunomodulatory parameters (white blood cell/WBC, red blood cell/RBC, phagocytic index/IP) and relative presentation survival/RPS was observed at week 4th after challenges with bacteria through intramuscularly injection (0.1 mL/fish). The number of white blood cell (WBC) was increased significantly (P <0.05) compared to controls without extract and it also happens with red blood cell (RBC), phagocytic index also experienced a significant increasing in fish fed with combined extracts compared to controls. The results of this study indicate that giving concoction of three extracts provides the best protection (RPS) against infection of A. hydrophila and P. fluorescent. The conclusion this research is concoction of SF:BP:ZZ in the ratio of 1:1:1 have a immunomodulatory effect in tilapia and increasing protection against bacterial infections

Toksisitas Produk Ekstrasellular (ECP) Streptococcus agalactiae pada Ikan Nila (Oreochromis niloticus) Hardi, Esti Handayani; Sukenda, Sukenda; Harris, Enang; Lusiastuti, Angela Mariana
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

This research aimed to know the toxicity of extracellular products (ECP) of Streptococcus agalactiae was tastedin cultured Nile tilapia (Oreochromis niloticus). Streptococcus agalactiae had two haemolytic types: β-haemolyticand non-haemolytic type. Toxicity test of ECP to know the virulancy factor of S. agalactiae was still limited. It wasfound that after tested on 15 fish weighing 15 g through intraperitoneal injection 0,1 ml/fish, both bacteria causedchanges in swimming pattern, palatability, external and internal anatomy macroscopically and microscopically.Extracellular products of S. agalactiae non-haemolytic type (BHIA and BHI 24 h) and β-haemolytic type (BHI 72 h)caused mortality 12 hours after injection and the mortality continued till day 7 th of culture. Whirling happened 96hours after injection with ECP S. agalactiae β-haemolytic type (BHIA 72 h incubation) whereas injection with ECP(BHI 24 h) on 72 h after injection and continued untill day 7 th. Behavior disease signs caused by S. agalactiaeoccured on eyes. There were opacity, purulens, eye shrink, lateral and bilateral exopthalmia and haemorrhage oninfected-fish. Silver staining of sodium dodecyl sulphate-polyacrylamide gels to S. agalactiae revealed thatpredominant 51.8-69.6 kDa bands were present in BHIA ECP fraction. The 69.6 kDa was absent from the BHI ECP.Total protein on non-haemolytic S. agalactiae ECP are 28.18 ppm on BHIA medium and 13.64 ppm on BHI medium.Whereas β-haemolytic S. agalactiae ECP are 2.73 ppm on BHIA medium and 8.18 ppm on BHI medium. Concentrationof protein in ECP was one of factor that caused non-haemolytic S. agalactiae more virulent than β-haemolytic type.The conclusion from the research that ECP was virulent factor on β-haemolytic and non-haemolytic S. agalactiaein fish which caused changes in behavior disease signs.

UJI IN VITRO GABUNGAN EKSTRAK TANAMAN DARI KALIMANTAN, INDONESIA TERHADAP BAKTERI PATOGEN PADA IKAN NILA Esti Handayani Hardi; Gina Saptiani; Nurkadina Nurkadina; Irawan Wijaya Kusuma; Wiwin Suwinarti
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of this study was to evaluate the antibacterial activity of ethanol concoction (mixed extract) of three herbs plants, such as: Boesenbergia pandurata, Solanum ferox and Zingimber zerumbet against Aeromonas hydrophila, Pseudomonas sp. and both bacteria. The Plant extracts were obtained by using ethanol and the concentration of the extract was 600 mgL-1 of B. pandurata, 900 mgL-1 of S. ferox and 200 mgL-1 of Z. zerumbet. The inhibition zone was measured after 6, 12, 18, 24 h incubation at 30o C. The ratios of concoction S. ferox and B. pandurata (for 100 mL solution) were 90:10; 80:20; 70:30; 60:40; 50:50; 40:60; 30:70; 20:80; 10:90. The same ratios were made on the concoction of S. ferox and Z. zerumbet. The concoctions of S. ferox and B. pandurata in the ratio of 50:50 and 60:40, and the concoction of S. ferox and Z. Zerumbet with ratio 60:40 had higher antibacterial activity against A. hydrophila single isolate compared to the other concoctions. Furthermore, the concoction extract of S. ferox and B. pandurate 50:50 and the combined of S. ferox and Z. zerumbet ratio 50:50 and 90:10 were the best combination to inhibit the growth of a single bacterium Pseudomonas sp. The combined S. ferox and B. pandurata ratio 50:50; 10:90 and S. ferox mixed with Z. zerumbet ratio 50:50 and 40:60 were the best combination against the combined bacteria between A. hydrophila and Pseudomonas sp. The conclusion of this research was the combined extract of S. ferox and B. pandurata and S. ferox with Z. zerumbet are effective to suppress the growth of single or combination of A. hydropila and Pseudomonas sp.

Patogenesitas Bakteri Enterobacteriaceae pada Ikan Zebra (Danio rerio) Sebagai Hewan Model Widyaningsih Rahayu; Esti Handayani Hardi; Gina Saptiani
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Enterobacteriaceae are Gram negative bacteria contain endotoxin and exotoxins which arerequirements for pathogenic bacteria and act as opportunistic pathogens. The purpose of this research was to determine the ability of Enterobacteriaceae bacteria to infect zebrafish (Danio rerio) by observing anatomical pathology, mortality, time death and cumulative time of death. The method used in thisresearch is a completely randomized design method (CRD). This research was conducted in three stages, first preparation of zebrasfish as animal model by average size 3-5cm and reach three months old. Second, bacterial cultures from 10 species of Enterobacteriaceae were Escherichia coli 1, E. coli 2, Enterobactercloacae 1, Acinetobacter calcoaceticus, Enterobacter amnigenus 1, Enterobacter amnigenus 1, Escherichia coli 3, Pantoea spp., E. cloacae 1, E. cloacae 2, E. cloacae 3, cultured in Brain Heart Infused Broth (BHIB) and later media washed with Phospate Buffer Saline (PBS) 0.45%. The third stage was bacterial infection to zebrafish using immersion method with each treatment repeated 3 times and observed for 120 hours. The results showed 10 species that used caused death (mortality), the highest mortality in fish that infected with E. coli 2 and E. cloacae 1 with a percentage of 66.67% infected by E. coli 2 and E. cloacae 1. Anatomicalpathology in the external organs and internal organs showed post-infectious symptoms. Infection occurred with mean time to death of 12-86 hours, as long as the cumulative time of fish death caused by E. coli 2, A.cinetobacter calcoaceticus and E. cloacae 1 causing death from 24 hours after infection until the end ofobservation.

Ekstrak Daun Pepaya dan Kangkung untuk Meningkatkan Daya Tetas Telur dan Kelangsungan Hidup Larva Lele (EXTRACTS OF CARICA PAPAYA AND IPOMOEA AQUATICA FOR IMPROVING EGG HATCHABILITY AND LARVAL VIABILITY OF CATFISH) Gina Saptiani; Esti Handayani Hardi; Catur Agus Pebrianto; Agustina Agustina
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.322 KB)

This research was aimed to investigate the potential use of leaf extract of Carica papaya and Ipomoeaaquatica lto improve egg hatchability and larval viability of catfish. Dried leaves of Carica papaya andIpomoea aquatica were macerated and extracted in water and ethanol. Eggs and larvae were tested in theaquarium size of 10 L with a a diameter of 28 cm. The extracts in concentration 600, 800 and 1.000 ppmwere tested on the egg hatchability of catfish with immersion method, and challed with Aeromonashydrophyla, Pseudomonas sp., and Saprolegnia spp. The extracts in concentration 800 and 1.000 ppm weretested on the larval viability with immersion method, and challed with pathogens. Water or ethanolextract of Carica papaya and Ipomoea aquatica can improve egg hatchability 67±8% until 90±6% andlarval viability of catfish 77±0,5 until 90±9%. Eight hundred ppm ethanol extract of Carica papaya has thebest egg hatchability and 1000 ppm can improve larval viability of catfish.

Toksisitas Produk Ekstraseluler dan Intraseluler Bakteri Pseudomonas sp. pada Ikan Nila (Oreochromis niloticus) (TOXICITY OF EXTRACELLULAR AND INTRACELLULAR PRODUCT OF PSEUDOMONAS SP IN TILAPIA (OREOCHROMIS NILOTICUS) Esti Handayani Hardi; Catur Agus Pebrianto; Gina Saptiani
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.236 KB)

The aim of the research was to investigate the toxicity of extracellular product (ECP) and intracellularproduct (ICP) of Pseudomonas sp. on tilapia. A total of 40 tilapias weighing 15 grams were injected withECP and ICP. The ECP and ICP were harvested from Pseudomonas sp. culture on two kinds of culturemedia and different time of incubation. The Pseudomonas was cultured on trypticase soy agar (TSA) andtrypticase soy broth (TSB) and incubated at 24, 48 and 72 hours. The slurry of the bacteria was centrifugedat 10000 g, for 30 minutes on 4oC to get ECP and in room temperature to get ICP. The supernatant wasfiltered with 0.45 ?m paper mesh. A hundred percent mortality was found in tilapia six hours postinjection with ICP (72 hours) whereas tilapias were injected with ECP caused 60% mortality in 12 hours.The tilapia showed whirling at 24 hour post injected with ECP of Pseudomonas sp which was cultured inTSA for 48 hours incubated. Opacity of the cornea and exopthalmia were occurred at 48 hours postinjection of ECP and ICP which were harvested from both media. Injection of ICP caused pathologychanges on internal organ of fish i.e. pale appearance of spleen and liver. In conclusion, the ECP and ICPwere a virulence factors of Pseudomonas sp. and the ICP seem more pathogenic and caused mortality thanECP. Both culture media and time of incubation influence of ECP and ICP production. The ECP and ICPwhich were harvested from Pseudomonas sp incubate for 24-48 hour more virulent than 72 hour.

Kandidat Vaksin Potensial Streptococcus agalactiae untuk Pencegahan Penyakit Streptococcosis pada Ikan Nila (Oreochromis niloticus) (POTENTIAL VACCINE CANDIDATE OF STREPTOCOCCUS AGALACTIAE FOR PREVENT STREPCOCOCOSIS ON NILA TILAPIA (OREOCHROMIS NILOTICUS) Esti Handayani Hardi; Sukenda .; Enang Harris; Angela Mariana Lusiastuti
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.319 KB)

The effectiveness of Streptococcus agalactiae vaccine in tilapia (Oreochromis niloticus) was evaluatedfor prevention of streptococcal disease. The vaccine was prepared using formalin-killed whole cell andconcentrated extracellular products/ECP (62.3 and 55.8 kDa) of â-haemolityc isolate and 62.3; 55,8 and51.8 kDa protein of non-haemolityc ECP of S. agalactiae. Vaccination and challenged (103 colony-formingunits (CFU)/fish of â-haemolityc and 105 CFU/fish of non-haemolityc S. agalactiae) trial was conducted byintraperitonial (IP) injection into fish with average body weight of 15 g. Fish were vaccinated with wholecell, ECP and mixed (whole cell and ECP) vaccine. Tilapia vaccinated with whole cell of â-haemolitycisolate had a relative percent survival (RPS) rates higher than those of ECP â-haemolityc vaccine. However,fish vaccinated with mixed (whole cell and ECP) of â-haemolityc has a better protection rates as comparedto those of two type of S. agalactie infection. Whereas those vaccinated with mixed (whole cell non-haemolitycand ECP of â-haemolityc) vaccine has protection rate of 79% from â-haemolityc and 42% from non-haemolitycinfection. Tilapia vaccinated with whole cell of non-haemolityc was only able to protect fish from non-haemolityc infection and was unable to protect fish from other types. Tilapia vaccinated with ECP non-haemolityc had a worse RPS than others vaccines in which mix whole cell and ECP vaccine of non-haemolitychad a protection 50-56% from S. agalactiae infection. Whereas vaccinated with mixed (whole cell â-haemolityc and ECP of non-haemolityc) vaccine showed a better to protect from â-haemolityc than non-haemolityc infection. It showed thatvaccination with mixed (whole-cell and extracellular product) vaccineof S. agalactiae â-haemolityc was more effective to protect tilapia against Streptococcosis.

Karakteristik dan Patogenisitas Streptococcus Agalactiae Tipe ?-hemolitik dan Non-hemolitik pada Ikan Nila Esti Handayani Hardi; Sukenda -; Enang Harris; Angela Mariana Lusiastuti
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1022.628 KB)

Streptococcus agalactiae was isolated from cultured Nile tilapia (Oreochromis niloticus) in Cirata gulfand Klaten. The isolates were Gram positive cocci, oxidative fermentative positive, motility, and catalasenegative, grown on media containing NaCl 6.5%, ?-haemolytic and non-haemolytic. Two types of S. agalactiae(?-haemolytic and non-haemolytic) are different from their variety of sugars fermentation. Strains ?-haemolytic can ferment more sugars, including arabinose, sorbitol, lactose, and trehalose. Experimentalinfectivity trials on Nile tilapia (size 15 g), non-haemolytic type showed more virulent. This type causedfaster mortality, more severe behavior changes, and pathology changes than â-haemolytic type. NonhemoliticS. agalactiae caused 48% mortality 6-24 hours after injection, whereas â-haemolitic type caused17% mortality which it occured in 48 hours after injection (mortality of fish control 2,22%). Behaviordisease signs caused by non-haemolitic S. agalactiae started to happen 6 hours after injection whereas 12hours in ?-haemolytic type infection. Histopatological changes were observed on fish eye, spleen, andbrain. Hyperaemia, hyperthrophi, degeneration, and necrosis were also found on infected fish. Thisresearch was concluded that non-haemolytic of S. agalactiae was more virulent than ?-haemolytic.

Peningkatan Efikasi Vaksinasi pada Ikan Nila (Oreochromis niloticus) dengan Penambahan Ekstrak Tanaman Terung Asam dan Lempuyang Esti Handayani Hardi; Komsanah Sukarti; Maulina Anggridini
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.722 KB)

This research was aimed to evaluate the Solanum ferox/SF and bitter ginger (Zingiber zerumbet/ZZ) in increasing the effectiveness of the Pseudomonas sp. (EP-02) vaccine through immersion in tilapia (Oreochromis niloticus). The dose used was 600 mg.L-1 of SF; 200 mg.L-1 of ZZ and vaccine bacterial density used 108 CFU.mL-1, the ratio between vaccine and extract was 1: 1: 1. The test were begun with immersion the tilapia in the vaccine and extract for 20 minutes, the challenges test was done on day 7th (d7), 14th (d14) and 21th (d21) post-vaccination with the combined bacteria Aeromonas hydrophila (EA-01) and Pseudomonas fluorescens (EP-01) (concentration of each bacteria was 105 CFU.mL-1) through intramuscular injection of 0.1 mL each fish. Abnormal swimming patterns, pathology of anatomy, total mortality, survival rate, RPS, were measured at week 2 after the challenge test. Application of SF and ZZ extracts in vaccination can increase the non specific (haematology parameters) and specific immunity (phagocytic index and antibody) since d7 of vaccination. While the increasing fish immunity in vaccine groups occurred in d14 post vaccination. The extract administration in vaccination reduced the fish mortality; increased SR and RPS of tilapia faster than the vaccination group. In conclusion, addition of SF and ZZ extracts can increase the efficacy of vaccines faster and more effectively from day 7th of infection.

Co-Authors , Agustina Agriandini, Maulina Agustina Al Hidayah, Rahmawati Alfian Alfian ALIM ISNANSETYO Amalia, Rizqi Andi Noor Asikin Andre Jolpano Angela Mariana Lusiastuti ANGELA MARIANA LUSIASTUTI Apriza Apriza, Apriza Ardana Kurniaji Aryani, Retno Asih, Supadmi Asmir, Asmir Aziza Aziza Banin, Maghfirotin Marta Catur Agus Pebrianto Catur Agus Pebrianto Catur Agus Pebrianto, Catur Diana, Rita Dosim Enang Harris Enang Harris Fikri Ardhani Fitriyana Fitriyana, Fitriyana Gina Saptiani Gina Saptiani Gina Saptiani Gina Saptiani Gina Septiani Grizelda Grizelda Halim, Aldi M. Haris Retno Susmiati Haris Retno Susmiyati Henny Pagoray Henny Pagoray, Henny Heru Kusdianto Hetty Manurung Hetty Manurung, Hetty Ida Ayu Putu Sri Widnyani Irawan Wijaya Kusuma, Irawan Wijaya Isriansyah Isriansyah Jolpano, Andre Komsanah Sukarti Komsanah Sukarti Krishna Purnawan Candra Kusuma, I W kusuma, W Lina Warlina Maria Damanik Marwati Marwati Maulida Rachmawati Maulida Rachmawati Maulina Agriandini Maulina Anggridini Mira Mawardi Naim, Muhammad Najli, Najli Niklani, Andi Nugroho, RA Nugroho, Rudi Agung Nurkadina Nurkadina Nurul Puspita Palupi Nurul Puspita Palupi Panggulu Ahmad Ramadhani Utoro Rahayu, Widianingsih Rahmah, Nur Rini Renitasari, Diana Putri Retno Aryani Reynata, Ricko Rita Diana Rita Diana Rizki Tri Handayani Rizki Tri Handayani, Rizki Rudi Agung Nugroho Rudianto Rudianto Rudianto Rudianto Rudy Agung Nugroho Ryan Anjani Sukenda . Sukenda . Supryady, Supryady Suwinarti, Wiwin Triesna Hidayanti Triesna Hidayanti, Triesna Wanda Rachmawati Warkhatun Najidah Wendrika, Wendrika Widyaningsih Rahayu Wiwik Harjanti Yulian Andriyani Yulian Andriyani, Yulian Yunarty Yunarty, Yunarty

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