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Journal : Jurnal Veteriner

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Ekstrak Daun Pepaya dan Kangkung untuk Meningkatkan Daya Tetas Telur dan Kelangsungan Hidup Larva Lele (EXTRACTS OF CARICA PAPAYA AND IPOMOEA AQUATICA FOR IMPROVING EGG HATCHABILITY AND LARVAL VIABILITY OF CATFISH) Gina Saptiani; Esti Handayani Hardi; Catur Agus Pebrianto; Agustina Agustina
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research was aimed to investigate the potential use of leaf extract of Carica papaya and Ipomoeaaquatica lto improve egg hatchability and larval viability of catfish. Dried leaves of Carica papaya andIpomoea aquatica were macerated and extracted in water and ethanol. Eggs and larvae were tested in theaquarium size of 10 L with a a diameter of 28 cm. The extracts in concentration 600, 800 and 1.000 ppmwere tested on the egg hatchability of catfish with immersion method, and challed with Aeromonashydrophyla, Pseudomonas sp., and Saprolegnia spp. The extracts in concentration 800 and 1.000 ppm weretested on the larval viability with immersion method, and challed with pathogens. Water or ethanolextract of Carica papaya and Ipomoea aquatica can improve egg hatchability 67±8% until 90±6% andlarval viability of catfish 77±0,5 until 90±9%. Eight hundred ppm ethanol extract of Carica papaya has thebest egg hatchability and 1000 ppm can improve larval viability of catfish.
Toksisitas Produk Ekstraseluler dan Intraseluler Bakteri Pseudomonas sp. pada Ikan Nila (Oreochromis niloticus) (TOXICITY OF EXTRACELLULAR AND INTRACELLULAR PRODUCT OF PSEUDOMONAS SP IN TILAPIA (OREOCHROMIS NILOTICUS) Esti Handayani Hardi; Catur Agus Pebrianto; Gina Saptiani
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the research was to investigate the toxicity of extracellular product (ECP) and intracellularproduct (ICP) of Pseudomonas sp. on tilapia. A total of 40 tilapias weighing 15 grams were injected withECP and ICP. The ECP and ICP were harvested from Pseudomonas sp. culture on two kinds of culturemedia and different time of incubation. The Pseudomonas was cultured on trypticase soy agar (TSA) andtrypticase soy broth (TSB) and incubated at 24, 48 and 72 hours. The slurry of the bacteria was centrifugedat 10000 g, for 30 minutes on 4oC to get ECP and in room temperature to get ICP. The supernatant wasfiltered with 0.45 ?m paper mesh. A hundred percent mortality was found in tilapia six hours postinjection with ICP (72 hours) whereas tilapias were injected with ECP caused 60% mortality in 12 hours.The tilapia showed whirling at 24 hour post injected with ECP of Pseudomonas sp which was cultured inTSA for 48 hours incubated. Opacity of the cornea and exopthalmia were occurred at 48 hours postinjection of ECP and ICP which were harvested from both media. Injection of ICP caused pathologychanges on internal organ of fish i.e. pale appearance of spleen and liver. In conclusion, the ECP and ICPwere a virulence factors of Pseudomonas sp. and the ICP seem more pathogenic and caused mortality thanECP. Both culture media and time of incubation influence of ECP and ICP production. The ECP and ICPwhich were harvested from Pseudomonas sp incubate for 24-48 hour more virulent than 72 hour.
Co-Authors Asmir, Asmir Esti Handayani Hardi Gina Saptiani Gina Saptiani Gina Saptiani Ida Ayu Putu Sri Widnyani