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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal MIPA EUGENIA
Jantje Pelealu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Mathematics Physics

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Isolation and Identification Of Triterpenoid Saponin From Baringtonia asiatica Kurz Seeds. Tanor, Meity N; Abadi, Abdul Latief; Rahardjo, Bambang Tri; Pelealu, Jantje
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.4.2.%x

Abstract

The study aimed to identify the content of the triterpenoid saponin compound from Barringtonia asiatica seeds collected from coastal of Malalayang beach, Manado, North Sulawesi. The method of extraction was the maceration with methanol solvent. The identification of the isolate was by thin layer chromatography, column chromatography, and GCMS. The phytochemistry test result of B. asiatica seed extract contained the compounds of alkaloid, saponin, and tannin. Then, the compound was separated by a thin layer chromatography method with a solvent system of methanol:chloroform:water. It produced three nodes that were spread around polar (Rf 0.24), semipolar (Rf 0.6) and non-polar (Rf 0.78) areas. Meanwhile, the process of column chromatography could only separate two chemical components namely semipolar (Rf 0.6) and polar (Rf 0.76). The identification with GCMS resulted in three compounds of Triterpenoid saponins, namely 2.4-bis-(1.1-dimethyl ethyl); 4-Dodecylphenol; and 2.6 bis-(1.1-dimethyl ethyl-4-methyl).
Co-Authors Abdul Latief Abadi Ariyane O.S. Siwu Bambang Tri Rahardjo Caroulus S. Rante Christian L. Kaparang Christina Salaki Claudius F. Kairupan, Claudius F. Emmy Senewe Evi Paendong Evie Paendong Feybra S. Sigarlaki Frangky J. Paat Henny V.G. Makal Herens A. Parera Hetsi A. Kaurow, Hetsi A. J. Manueke, J. James B. Kaligis Jandrio H. Moonik Jimmy Rimbing Juliet M.E. Mamahit Jusuf Manueke Max Tulung Meity Nelltje Tanor Moulwy F. Dien Natalia Samba Noni N. Wanta Robert W. Tairas, Robert W. Salaki, Ch L