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All Journal Agrotech Journal Jurnal Biosilampari: Jurnal Biologi
Fuadiska Salamena
Pattimura University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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GENETIC CHARACTERIZATION OF GALOBA DURIAN (AMONUM SPP.) IN AMBON ISLAND BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) Fuadiska Salamena; Adriana Hiariej; Cecilia Anna Seumahu
Agrotech Journal Vol 3, No 1 (2018): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v3i1.524

Abstract

Galoba durian is one of the endemic floras from Maluku. Galoba durian is a species belonging to the Amomum genera Zingiberaceae family. It is also used as a medicinal plant for waist and kidney diseases. Based on the color of the fruit, galoba durian is divided into two nmely red galoba durian and green galoba durian. Distribution of this plant in Ambon can found in a few places such as highland and coastal area. Different locations influence phenotypic of plants, but may not show different genetic characteristic. Genetic diversity can detected by molecular markers. Genetic characterization from galoba durian using RAPD markers has not been done before. This study aimed to analyze genetic diversity from galoba durian using molecular markers RAPD. Samples of plants are used red galoba durian from Hatu and green galoba durian from Hatalae. The result of the first study, characterization of the morphology of the galoba durian, showed that both galoba have almost similar characteristics. Further DNA was tested by qualitative and quantitative. Result shows good qualitative and quantitative of DNA genomic. The second study was amplification by PCR-RAPD. DNA amplifications were performed using 3 primers out of 9 screened random primers. The primers selection was based on hight polymorphism. DNA amplification has 36 bands which were 100% polymorphic. The size of each bands from visualization of agarose was determined by linear regression. Number of band amplified was range from 120 to 1612 bp. Polymorphic band of RAPD showed the highest  genetic diversity. It can be concluded that the two plants of galoba durian are different species
Potential and Synergy Evaluation of Indigenous Biosurfactant-Producing Bacteria from Abandoned Oil Wells for Microbial Enhanced Oil Recovery (Meor) Technology Development Brian Saputra Manurung; Windy Natalia Nusaly; Abdul Mahid Ukratalo; Monalisa Pertiwi Jeriska Taihuttu; Fuadiska Salamena
Jurnal Biosilampari : Jurnal Biologi Vol 7 No 2 (2025): Biosilampari: Jurnal Biologi
Publisher : Universitas PGRI Silampari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62112/biosilampari.v7i2.224

Abstract

Petroleum is a non-renewable natural resource that continues to be used in many applications. However, the availability of crude oil ready for use is decreasing due to the low productivity of oil wells, one of which is caused by its high viscosity. This needs to be resolved to meet the demand for crude oil. This study aims to determine the potential of eight petrophilic bacteria in producing biosurfactants and to determine their interaction to be applied to Microbial Enhanced Oil Recovery technology. The research design in this study was a Factorial Randomized Group Design with two types of treatments: 8 types of bacteria and 4 different temperatures. The experimental units were 32, with 2 replications of each, resulting in 64 experimental units.  The observation variables in this study were the diameter of the clear zone and the interaction of bacteria. Clear zone diameter was analyzed using Analysis of Variance, then continued with Duncan's New Multiple Range Test at 5%. This study showed the formation of clear zones as an indicator of the production of biosurfactants influenced by temperature. Brevundimonas   diminuta and P. peli have superior potential in forming clear zones in hemolytic assay than other bacteria, with clear zone diameters of 26.805 mm and 26.040 mm, respectively, at 44°C of incubation. Three bacteria have a high percentage of synergy in this study of 50%, which synergized with 4 other types of bacteria.
Co-Authors Abdul Mahid Ukratalo Adriana Hiariej, Adriana Brian Saputra Manurung CECILIA ANNA SEUMAHU Monalisa Pertiwi Jeriska Taihuttu Windy Natalia Nusaly