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All Journal Agrotech Journal Jurnal Malikussaleh Mengabdi Jurnal Biosilampari: Jurnal Biologi
Fuadiska Salamena
Pattimura University
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Social Sciences Other

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GENETIC CHARACTERIZATION OF GALOBA DURIAN (AMONUM SPP.) IN AMBON ISLAND BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) Fuadiska Salamena; Adriana Hiariej; Cecilia Anna Seumahu
Agrotech Journal Vol 3, No 1 (2018): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v3i1.524

Abstract

Galoba durian is one of the endemic floras from Maluku. Galoba durian is a species belonging to the Amomum genera Zingiberaceae family. It is also used as a medicinal plant for waist and kidney diseases. Based on the color of the fruit, galoba durian is divided into two nmely red galoba durian and green galoba durian. Distribution of this plant in Ambon can found in a few places such as highland and coastal area. Different locations influence phenotypic of plants, but may not show different genetic characteristic. Genetic diversity can detected by molecular markers. Genetic characterization from galoba durian using RAPD markers has not been done before. This study aimed to analyze genetic diversity from galoba durian using molecular markers RAPD. Samples of plants are used red galoba durian from Hatu and green galoba durian from Hatalae. The result of the first study, characterization of the morphology of the galoba durian, showed that both galoba have almost similar characteristics. Further DNA was tested by qualitative and quantitative. Result shows good qualitative and quantitative of DNA genomic. The second study was amplification by PCR-RAPD. DNA amplifications were performed using 3 primers out of 9 screened random primers. The primers selection was based on hight polymorphism. DNA amplification has 36 bands which were 100% polymorphic. The size of each bands from visualization of agarose was determined by linear regression. Number of band amplified was range from 120 to 1612 bp. Polymorphic band of RAPD showed the highest  genetic diversity. It can be concluded that the two plants of galoba durian are different species
Potential and Synergy Evaluation of Indigenous Biosurfactant-Producing Bacteria from Abandoned Oil Wells for Microbial Enhanced Oil Recovery (Meor) Technology Development Brian Saputra Manurung; Windy Natalia Nusaly; Abdul Mahid Ukratalo; Monalisa Pertiwi Jeriska Taihuttu; Fuadiska Salamena
Jurnal Biosilampari : Jurnal Biologi Vol 7 No 2 (2025): Biosilampari: Jurnal Biologi
Publisher : Universitas PGRI Silampari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62112/biosilampari.v7i2.224

Abstract

Petroleum is a non-renewable natural resource that continues to be used in many applications. However, the availability of crude oil ready for use is decreasing due to the low productivity of oil wells, one of which is caused by its high viscosity. This needs to be resolved to meet the demand for crude oil. This study aims to determine the potential of eight petrophilic bacteria in producing biosurfactants and to determine their interaction to be applied to Microbial Enhanced Oil Recovery technology. The research design in this study was a Factorial Randomized Group Design with two types of treatments: 8 types of bacteria and 4 different temperatures. The experimental units were 32, with 2 replications of each, resulting in 64 experimental units.  The observation variables in this study were the diameter of the clear zone and the interaction of bacteria. Clear zone diameter was analyzed using Analysis of Variance, then continued with Duncan's New Multiple Range Test at 5%. This study showed the formation of clear zones as an indicator of the production of biosurfactants influenced by temperature. Brevundimonas   diminuta and P. peli have superior potential in forming clear zones in hemolytic assay than other bacteria, with clear zone diameters of 26.805 mm and 26.040 mm, respectively, at 44°C of incubation. Three bacteria have a high percentage of synergy in this study of 50%, which synergized with 4 other types of bacteria.
CRISPR/Cas9: Pengenalan Aplikasi CRISPR/Cas9 untuk Siswa SMK Negeri 6 Ambon Fuadiska Salamena; Windi Mose; Edwin Thomas Apituley; Kristi Lenci Patty
Jurnal Malikussaleh Mengabdi Vol. 5 No. 2 (2026): Jurnal Malikussaleh Mengabdi, April 2026
Publisher : LPPM Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/jmm.v5i2.25936

Abstract

Kegiatan PkM Program studi Bioteknologi FST dilakukan di SMK Negeri 6 Ambon yang dilaksanakan pada tanggal 28 Oktober 2025. SMK Negeri 6 Ambon yang merupakan salah satu sekolah menengah kejuruan di Kota Ambon. Kegiatan PKM ini bertujuan memperkenalkan teknologi penyuntingan gen CRISPR/Cas9 kepada siswa SMK Negeri 6 Ambon melalui sosialisasi dan media pembelajaran interaktif. Melalui sosialisasi berupa presentasi digital dan simulasi sederhana, siswa diperkenalkan pada konsep serta penerapan teknologi bioteknologi modern dalam bidang kesehatan. Rendahnya literasi bioteknologi tingkat lanjut di kalangan siswa SMK Negeri 6 Ambon dapat memberikan kesenjangan kompetensi antara lulusan SMK dengan kebutuhan dunia kerja yang semakin mengarah pada pemanfaatan teknologi berbasis genetika. Hal ini dibuktikan dari survei awal yang menunjukkan 74% siswa tidak mengetahui pendekatan CRISPR/Cas9. Program ini membantu meningkatkan pemahaman siswa tentang literasi bioteknologi. Survei akhir menunjukkan bahwa 85% siswa sudah paham mengenai metode CRISPR/Cas9 dan 77,8% siswa sudah dapat menyebutkan contoh penerapan metode CRISPR/Cas9 dikehidupan sehari-hari. Secara keseluruhan menunjukkan adanya minat siswa untuk mempelajari lebih lanjut tentang literasi bioteknologi. Program ini meningkatkan literasi bioteknologi, menumbuhkan minat terhadap inovasi ilmiah, dan mempersiapkan generasi muda vokasi agar siap menghadapi perkembangan teknologi kesehatan masa depan. Namun hal ini perlu dilakukan evaluasi lanjutan untuk terus menambah minat dan literasi siswa terhadap ilmu bioteknologi sehingga dapat memenuhi kebutuhan dan tantangan SDM unggul yang cakap, handal, dan berintegritas dalam melakukan pekerjaan-pekerjaan untuk mendukung pembangunan Maluku lebih baik lagi ke depannya
Co-Authors Abdul Mahid Ukratalo Adriana Hiariej, Adriana Brian Saputra Manurung CECILIA ANNA SEUMAHU Edwin Thomas Apituley Kristi Lenci Patty Monalisa Pertiwi Jeriska Taihuttu Windi Mose Windy Natalia Nusaly