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Breadfruit Flour, Food to Lower Blood Sugar Levels in Mice Model Diabetes Mellitus Martha Kaihena; Abdul Mahid Ukratalo; Maria Nindatu; Deford Christy Birahy
Jurnal Health Sains Vol. 4 No. 3 (2023): Journal Health Sains
Publisher : Syntax Corporation Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/jhs.v4i3.866

Consumption of foods with high amylose content (>25%) as well as a low glycemic index (<55) is able to improve the insulin sensitivity of people with diabetes mellitus. Breadfruit has in about 23-60 which means that breadfruit is safe to consume. The purpose of this research is to find out the levels of reduced sugar, amylose, and blood sugar levels mice after being fed breadfruit flour. This research used a Complete Randomized Design using 5 treatments namely normal control group (KN), negative control (K-), positive control (K+), STZ + breadfruit flour dose 5 g (P1) and STZ + breadfruit flour dose 10 g (P2). Breadfruit is taken and then processed into breadfruit flour, after which testing of reduced sugar content and amylose breadfruit flour. Before treatment, all mice were measured for initial glucose levels. After that in groups K (-), K (+), P1, and P2 injected STZ on days 1, 2, and 3. After 3 days, the blood glucose level (post-STZ) was measured again, then compared to the blood glucose level on the first day before being given STZ. If there is an increase in blood glucose levels of mice that is to ±128 mg / dL then mencit is considered to be diabetic. Then in group K (+) drug given Metformin, P1 and P2 were fed breadfruit flour with a predetermined dose for 7 days. The results showed that the sugar content of reduced breadfruit was cut by 3.26%. While the level of amylose in breadfruit flour is 19.3%. Breadfruit flour can also lower the sugar content of mice.

Introducing Indonesian endemism, its threat and conservation solution for SMA Negeri 10 students in Kamarian Village, West Seram Regency, Maluku Handy Erwin Pier Leimena; Cecilia Anna Seumahu; Dece Elizabeth Sahertian; Abdul Mahid Ukratalo; Martha Kaihena
Unram Journal of Community Service Vol. 5 No. 1 (2024): March
Publisher : Pascasarjana Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/ujcs.v5i1.585

West Seram Regency on Seram Island in Maluku Province, Indonesia has extraordinary biodiversity and is the habitat of several endemic species of Maluku and Indonesia. As a form of implementation of the dharma of community service, socialization was conducted with students of SMA Negeri 10 in Kamarian Village, West Seram Regency, Maluku about Indonesian endemism, its threats and conservation. The socialization was attended by 38 students. The socialization results indicate that 91% of students demonstrated a good understanding of endemic species, and 82% of students obtained information on endemic species from this activity. Additionally, students were able to identify 11 Indonesian endemic species, as well as the threats and efforts to protect them. This activity has the potential to increase environmental awareness among school students, the nation's young generation.

EFFECTIVENESS OF Sargassum sp EXTRACT IN REDUCE BLOOD SUGAR LEVELS AND ACCELERATE WOUND HEALING ON THE SKIN OF DIABETES MELLITUS MICE (Rattus novergicus) Kaihena, Martha; Umagapi, Muhammad Cesar; Kaliky, Arief Rahman Shabri; Tarangi, Filep Marfil; Ukratalo, Abdul Mahid
RUMPHIUS Vol 6 No 2 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i2p093-100

Diabetes Mellitus is a metabolic disorder characterised by elevated blood sugar levels beyond normal ranges, resulting from reduced secretion of the hormone insulin by pancreatic β cells and/or impaired insulin action. Diabetes therapy is often overseen by regulating blood glucose levels regularly and preventing or reducing the risk of complications. Sargassum sp is a variety of brown seaweed native to Indonesia with possible antioxidant and antidiabetic properties. This study seeks to evaluate the efficacy of Sargassum sp extract in lowering blood sugar levels and speeding up wound healing on the skin of diabetic mice. This is an experimental study that uses mice as experimental subjects. The mice were categorised into 5 groups (K-, K+, P1, P2, and P3). Their initial blood sugar levels were recorded, a skin incision was performed, and STZ was administered. When blood sugar levels rise, the K+ group receives metformin; on the other hand, P1, P2, and P3 are administered Sargassum sp extract at a specified dosage. The data were examined using ANOVA and SPSS. The analysis revealed that the water content of Sargassum sp was 4.32%, and the yield value of the concentrated extract was 8.75%. The ethanol extract of Sargassum sp has been revealed to lower blood sugar levels and speed up the wound healing process in mice with diabetes mellitus. The effects observed include decreased blood sugar levels and enhanced wound healing percentage, which are dose-dependent.

Potential and Synergy Evaluation of Indigenous Biosurfactant-Producing Bacteria from Abandoned Oil Wells for Microbial Enhanced Oil Recovery (Meor) Technology Development Brian Saputra Manurung; Windy Natalia Nusaly; Abdul Mahid Ukratalo; Monalisa Pertiwi Jeriska Taihuttu; Fuadiska Salamena
Jurnal Biosilampari : Jurnal Biologi Vol 7 No 2 (2025): Biosilampari: Jurnal Biologi
Publisher : Universitas PGRI Silampari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62112/biosilampari.v7i2.224

Petroleum is a non-renewable natural resource that continues to be used in many applications. However, the availability of crude oil ready for use is decreasing due to the low productivity of oil wells, one of which is caused by its high viscosity. This needs to be resolved to meet the demand for crude oil. This study aims to determine the potential of eight petrophilic bacteria in producing biosurfactants and to determine their interaction to be applied to Microbial Enhanced Oil Recovery technology. The research design in this study was a Factorial Randomized Group Design with two types of treatments: 8 types of bacteria and 4 different temperatures. The experimental units were 32, with 2 replications of each, resulting in 64 experimental units. The observation variables in this study were the diameter of the clear zone and the interaction of bacteria. Clear zone diameter was analyzed using Analysis of Variance, then continued with Duncan's New Multiple Range Test at 5%. This study showed the formation of clear zones as an indicator of the production of biosurfactants influenced by temperature. Brevundimonas diminuta and P. peli have superior potential in forming clear zones in hemolytic assay than other bacteria, with clear zone diameters of 26.805 mm and 26.040 mm, respectively, at 44°C of incubation. Three bacteria have a high percentage of synergy in this study of 50%, which synergized with 4 other types of bacteria.

BIOLARVACIDE ACTIVITY OF ETHANOL EXTRACT OF KEDONDONG STEM (Spondias pinnata) AGAINST Aedes aegypti Moniharapon, Debby D; Ukratalo, Abdul Mahid; Wisnanda, Bayu
RUMPHIUS Vol 1 No 1 (2019): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv1i1p012-017

Aedes aegypti mosquito is a vector for Dengue Hemorrhagic Fever. One effort to prevent the spread of the disease is vector control. The use of insecticides is a form of vector control in an effort to control DHF. There are two broad categories of insecticides that are often used as household insecticides, namely insecticides that function to kill insects and insecticides that function to repel insects. Kedondong (Spondias pinnata) which is a family of anacardiaceae which generally grows well in tropical climates. Kedondong bark contains saponins, alkaloids, and flavonoids which are secondary metabolites involved in defense mechanisms against attack by many microorganisms. This study aims to determine the biolarvicidal activity of the ethanol extract of kedondong stem bark in killing Aedes aegypti mosquito larvae. This study used 5 treatments and 3 replications. Each treatment contained 20 Aedes aegypti mosquito larvae. Observations were made for 24 hours with observation times of 0 hours, 6 hours, 12 hours, 18 hours and 24 hours. The time calculation starts after the larvae are inserted into the experimental bottle. The results showed that kedondong bark extract was able to kill Aedes aegypti mosquito larvae. The effective dose of ethanol extract of kedondong stem bark in killing Aedes aegypti mosquito larvae is 20 mg/mL.

Pelatihan Pengamatan Sel Dengan Mikroskop Olympus Cx-21 dan Dokumentasi Sel Menggunakan Smartphone Bagi Siswa Dan Guru Ma Al-Muluuk Persis Telaga Kodok, Leihitu, Kabupaten Maluku Tengah Seumahu, Cecilia Anna; Leimena, Handy Erwin Pier; Sahertian, Dece Elisabeth; Ukratalo, Abdul Mahid
Innovation for Community Service Journal Vol 2 No 1 (2024): April 2024
Publisher : Department of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/icsj.v2i1.12486

Penggunaan mikroskop sebagai alat dalam praktikum biologi menjadi sangat penting karena dapat mendukung keterampilan proses belajar sains. Tujuan dari kegiatan ini adalah untuk memberikan pemahaman yang mendalam kepada siswa dan guru mengenai penggunaan mikroskop Olympus CX-21 untuk pengamatan sel serta penggunaan smartphone untuk dokumentasi sel. Metode yang digunakan adalah ceramah, diskusi, tanya jawab serta demonstrasi untuk menyiapkan preparat segar tumbuhan, menggunakan mikroskop dengan benar serta mendokumentasi hasil pengamatan. Hasil yang dicapai adalah peserta mampu menggunakan mikroskop dan menyiapkan preparat segar tumbuhan dan mendokumentasikan serta memvisualisasikan hasil pengamatan. Peserta terlihat sangat antusias dan tertarik karena mampu mengamati jaringan tumbuhan langsung di bawah mikroskop.

Introducing Indonesian endemism, its threat and conservation solution for SMA Negeri 10 students in Kamarian Village, West Seram Regency, Maluku Leimena, Handy Erwin Pier; Seumahu, Cecilia Anna; Sahertian, Dece Elizabeth; Ukratalo, Abdul Mahid; Kaihena, Martha
Unram Journal of Community Service Vol. 5 No. 1 (2024): March
Publisher : Pascasarjana Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/ujcs.v5i1.585

West Seram Regency on Seram Island in Maluku Province, Indonesia has extraordinary biodiversity and is the habitat of several endemic species of Maluku and Indonesia. As a form of implementation of the dharma of community service, socialization was conducted with students of SMA Negeri 10 in Kamarian Village, West Seram Regency, Maluku about Indonesian endemism, its threats and conservation. The socialization was attended by 38 students. The socialization results indicate that 91% of students demonstrated a good understanding of endemic species, and 82% of students obtained information on endemic species from this activity. Additionally, students were able to identify 11 Indonesian endemic species, as well as the threats and efforts to protect them. This activity has the potential to increase environmental awareness among school students, the nation's young generation.

Bioprospeksi Ekstrak Etanol Gagang Cengkeh (Syzogium aromatica) Sebagai Biolarvasida Nyamuk Aedes aegypti Ayal, Yonri; Nindatu, Maria; Ukratalo, Abdul Mahid
Kalwedo Sains (KASA) Vol 2 No 1 (2021): Kalwedo Sains (KASA), Maret 2021
Publisher : Program Studi Di Luar Kampus Utama Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/kasav2i1p7-14

Vektor penyebab penyakit DBD adalah nyamuk Aedes aegypti yang dapat ditemukan hampir di seluruh provinsi di Indonesia karena nyamuk ini sangat mudah beradaptasi dengan lingkungan sekitarnya. Cengkeh (Syzogium aromatica) merupakan tanaman rempah asli Kepulauan Maluku. Masyarakat Maluku sering menggunakan gagang cengkeh dalam upaya mengusir nyamuk. Penggunaan gagang cengkeh dalam mengusir nyamuk umumnya dengan cara dibakar. Asap hasil pembakaran gagang cengkeh tersebut efektif dalam membasmi nyamuk. Tujuan dari penelitian ini adalah membuktikan ekstraketanol gagang cengkeh (Syzogium aromatica) dapat membunuh larva nyamuk Aedes aegypti. Penelitian menggunakan 5 perlakuan dan 3 kali ulangan. Tiga ratus larva nyamuk instar III dimasukan pada tiap kelompok perlakuan. Tiap wadah diisi 20 larva. Pengamatan aktivitas larvasida dilakukan setiap 4 jam sekali selama 24 jam. Data hasil pengamaan dianalisis menggunakan ANOVA pada taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa ekstrak etanol gagang cengkeh dapat membunuh larva nyamuk Aedes aegypti.Nilai LC50 ekstrak etanol gagang cengkeh sebesar 2,386 dengan batas bawah 0,319 dan batas atas 3,836.

Analisis Kandungan Gizi Dan Jumlah Mikroba Produk Ikan Kaleng Yang Dijual Di Beberapa Supermarket Di Kota Ambon Berdasarkan Lama Penyimpanan Kaihena, Martha; Ukratalo, Abdul Mahid
Kalwedo Sains (KASA) Vol 3 No 1 (2022): Kalwedo Sains (KASA), Maret 2022
Publisher : Program Studi Di Luar Kampus Utama Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/kasav3i1p55-62

Ikan kaleng merupakan salah satu jenis makanan yang cukup digemari karena praktis dan dapat disimpan dalam jangka waktu lama. Namun, komposisi kaleng yang terbuat dari logam berpotensi mencemari makanan sehingga berpotensi terdapat logam berat dan mikroba dalam produk ikan kaleng tersebut. Penelitian ini bertujuan untuk mengetahui kandungan gizi dan jumlah mikroba pada produk ikan kaleng yang dijual pada beberapa supermarket di Kota Ambon. Sampel ikan kaleng dibeli dari beberapa supermarket di Kota Ambon. Ikan kaleng yang digunakan adalah ikan kaleng jenis A, B dan C. Ikan kaleng yang telah dibeli dari supermarket kemudian di bawah ke Laboratorium Kimia Dasar untuk proses lebih lanjut. Ikan kaleng disimpan dengan lama penyimpanan 7, 14 dan 21 hari. Hasil penelitian menunjukkan bahwa penyimpanan produk ikan kaleng selama 7, 14 dan 21 hari menyebabkan peningkatan kadar protein dan kadar abu, sedangkan kadar air dan kadar lemak mengalami penurunan. Hasil uji terhadap kandungan bakteri pada produk ikan kaleng yang disimpan selama 7, 14 maupun 21 hari tidak menunjukkan adanya pertumbuhan mikroba.

Methanol Extract Alstonia scholaris L. R. Br as Hepatoprotective Mice (Mus musculus) Infected with Plasmodium berghei ANKA Strains Martha Kaihena; Maria Nindatu; Abdul Mahid Ukratalo
Jurnal Penelitian Pendidikan IPA Vol 9 No 8 (2023): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i8.4834

Alstonia scholaris L. R. Br is one of the traditional plants that contain natural antioxidant compounds which are thought to be able to repair damage to the liver cells of mice (Mus musculus) infected with Plasmodium berghei strain ANKA. This study aimed to determine the effect of methanol extract of Alstonia scholaris L. R. Br stem bark on levels of SGPT enzymes and liver cells of mice (Mus musculus) infected with Plasmodium berghei strain ANKA.Â Mice with a body weight of 20-30 g were infected with Plasmodium berghei as much as 0.1 ml per head and left until the percentage of parasitemia reached 1-5%. Then mice (Mus musculus) were given methanol extract of Alstonia scholaris L. R. Br stem bark at doses of 1, 10, 100, and 200 mg/kg BW for 4 consecutive days. After that, surgery was performed to take blood to observe SGPT enzyme levels and mice liver cells to be prepared with Hematoxylin Eosin (HE) staining. The results of ANOVA showed that the methanol extract of Alstonia scholaris L. R. Br stems bark doses of 1 mg/kg BW, 10 mg/kg BW, 100 mg/kg BW and 200 mg/kg BW could reduce SGPT enzyme levels and repair damage to the liver cells of mice infected with Plasmodium berghei ANKA strains

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