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All Journal HAYATI Journal of Biosciences BIOWALLACEA Jurnal Bioedukasi Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosel: Biologi Science and Education Jurnal Agritechno JURNAL BUDIDAYA PERTANIAN Agrotech Journal Rumphius Pattimura Biological Journal PENGAMATAN: Jurnal Pengabdian Masyarakat untuk Ilmu MIPA dan Terapannya
Adriana Hiariej, Adriana
Jurusan Biologi Fakultas MIPA Universitas Pattimura Ambon, Jl. Ir. M. Putuhena, Kampus Poka Ambon 97233 Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics Social Sciences Other

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PROFIL LINGKUNGAN TUMBUH PISANG TONGKAT LANGIT (Musa troglodytarum L.)DI KABUPATEN MALUKU TENGAH Hiariej, Adriana
BIOWALLACEA Vol 1, No 1 (2015): Bio Wallacea Vol 1 No 1
Publisher : Program Studi Biologi FMIPA Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Di semua propinsi di Indonesia tumbuh baik pisang liar maupun pisang yang sudah di budidayakan.Pisang Tongkat Langit dengan nama ilmiah Musa troglodytarum L. adalah jenis pisang yang tandannya tegak ke atas dan merupakan tanaman endemik di daearah Indonesia Timur, yaitu Maluku dan Papua. Oleh masyarakat Maluku dikenal dengan nama ”Pisang Tongka Langit”. Propinsi Maluku merupakan bagian dari wilayah Timur Indonesia, dijuluki daerah seribu pulau terdiri dari pulau-pulau kecil yang dipisahkan oleh laut.Wilayahnya bervariasi baik dari faktor geografis, edafik dan iklim.Perbedaan tersebut tentu sangat berpengaruh pada keragaman fenotipik dan genetik organisme termasuk pisang Tongkat Langit sebagai akibat interaksi dengan lingkungan.Penelitian ini bertujuan untuk mengetahui profil lingkungan tumbuh pisang Tongkat Langit di kabupaten Maluku Tengah.Metode eksplorasi dilakukan untuk mendeskripsi profil lingkungan meliputi suhu, kelembaban, salinitas sedangkan sampel tanah dari lingkungan tumbuh diambil untuk dianalisis, di laboratorium kimia tanah UB Malang dengan metode kering oven. Data profil lingkungan dideskripisikan secara deskriptif dan dianalisis dengan analisis cluster Indeks Bray Curtis. Hasil penelitian menunjukkan bahwa terdapat keragaman profil lingkungan tumbuh meliputi suhu (13-32°C), kelembaban (8-30%), salinitas( 0.21- 1.5 mS), pH 5.2-7.3), C.organik (1.83-5.23%), N. total (0.21-0.59%), C/N (8-12), P (2.22-23.19 mg kg-1), K, Na, Ca, Mg, KTK, jumlah basa (0.16-1.41, 0.24-1.82, 5.02-23.81, 1.07-3.32, 2.22-23.19, 8.64-27.68 me/100g), KB (43-92%), pasir (3-70%), debu (25-78%) dan liat (0-28%). Hasil analisis cluster menunjukkan bahwa terdapat 2 cluster utama dengan nilai similaritas 0,68-0,96. Dari keragaman profil lingkungan dapat dijelaskan ketersedian unsur hara sangat dipengaruhi oleh reaksi pH tanah, bahan organik, KTK, KB tekstur dan struktur tanah serta kehidupan mikroorganisme yang penting bagi pertumbuhan maupun produksi pisang tongkat langit.Untuk suhu, kelembaban, dan salinitas.dikatakan masih mendukung pertumbuhan pisang tongkat langit di kabupaten Maluku Tengah.  Kata Kunci :Musa troglodytarum L., Lingkungan tumbuh, Maluku Tengah
Genetic Relationship between Tongka Langit Bananas (Musa troglodytarum L.) from Galunggung and Maluku, Indonesia, Based on ITS2 Fenny Martha Dwivany; Giasintha Stefani; Agus Sutanto; Husna Nugrahapraja; Ketut Wikantika; Adriana Hiariej; Topik Hidayat; I Nyoman Rai; Nisrina Sukriandi
HAYATI Journal of Biosciences Vol. 27 No. 3 (2020): July 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.27.3.258

Abstract

Tongka Langit or Fe’i banana (Musa troglodytarum L.) has the T genome and a very high content of beta-carotene. It only grew and spread around the regions of Maluku islands and Papua. However, recently our team found this banana on the foot of mount Galunggung, West Java, so this raised the question about its origin. The objective of this study was to understand the genetic relationship between Tongka Langit from Galunggung and Maluku islands and compared it with other bananas with different genomes. Genetic diversity analysis was done using ITS2 DNA marker and dendrogram analysis showed three groups. From the comparison of the ITS2 sequences, there were no difference (100% identity) between the ITS2 sequence of Tongka Langit originating from Galunggung and Maluku. In conclusion, based on the ITS2 marker, the Tongka Langit were more distantly related to cultivars with A and B genomes, and there was no difference in the ITS2 sequence of Tongka Langit originating from Galunggung and Maluku. To the best of our knowledge, there is no previous report of genetic relationship between Tongka Langit from Galunggung and other regions.
Genetic Variability of Galoba Durian (Amomum spp) Center Mollucas and North Halmahera Based on RAPD Katerina Jagagura Peilouw; Adriana Hiariej; Anneke Pesik
Jurnal Penelitian Pendidikan IPA Vol. 8 No. 2 (2022): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v8i2.1353

Abstract

Galoba Durian is one of the endemic flora of Maluku, which is used as a medicinal plant and essential oil with red and green fruit colors. This study aimed to analyze the genetic variation of Galoba Durian in Central Maluku and North Halmahera based on the molecular marker of RAPD. This study began with a survey of sampling, isolation, and amplification of DNA using five RAPD primers. The results showed that all DNA bands were polymorphic as many as 67 with genetic similarity ranging from 20% - 31%, which grouped four samples into two groups. The first group is red Galoba Durian from North Halmahera (S1), red (S3) and green (S4) from Central Maluku, while the second group is green Galoba Durian from North Halmahera (S2).
Study of Stomata Characteristics of Plantain and Horn Plants AAB genome Clemens Sudiyono Narahayaan; Adriana Hiariej; Pieter Agusthinus Riupassa
Jurnal Penelitian Pendidikan IPA Vol. 8 No. 2 (2022): April
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v8i2.1382

Abstract

The study of stomata characteristics of plantain and horn plants has been carried out. This study aimed to compare the characteristics of plant stomata by plantain and horn banana AAB genome. Therefore, it is possible to find out the similarities and differences in characteristics between plantain and horn banana that have the same genome, including by stomata location, cover cell shape, stomatal type, number of epidermal cells, number of stomata cells, stomata length, stomata width, stomata area, stomata index, and stomatal density. Stomata observations were made by making an incision using the whole mount method. The results obtained were then analyzed descriptively qualitatively by describing the stomata characteristics of the plantain and horn banana plants
Korelasi Genotipe dan Fenotipe Antar Sifat Kuantitatif Pada Populasi Segregasi Transgresif Kacang Hijau Julian Timisela; Aca A Anakotta; Adriana Hiariej; Edizon Jambormias
JURNAL BUDIDAYA PERTANIAN Vol 16 No 1 (2020): Jurnal Budidaya Pertanian
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jbdp.2020.16.1.21

Abstract

Mung bean (Vigna radiata L. Wilczek) is one of the food plants as a source of vegetable protein for humans. One of these is a population of transgressive segregation resulting from crossing in mung beans. This research aims to estimate the correlation between genotype and phenotype values ​​between quantitative traits in the transgressive segregation population of mung beans. Genetic material was 11 lines of transgressive segregated in the selection generation S2:2 of varieties Mamasa Lere Butnem × Lasafu Lere Butnem crosses. Genotype correlation analysis was obtained from the decomposition of genetic variance components from the results of the analysis of variance according to a randomized complete design with sub-sampling, consisting of 13 genotypes (including check varieties) which were two replications. The results showed a genotype correlation between the quantitative traits of mung beans that indicated the possibility of direct selection of the populations of transgressive segregation lines on yields and indirectly through easily observable traits such as harvest age, number of pods, and number of seeds. Keywords: genetic correlation, mungbeans, quantitative traits ABSTRAK Kacang hijau (Vigna radiata L. Wilczek) merupakan salah satu tanaman pangan sebagai sumber protein nabati bagi manusia. Salah satu diantaranya adalah populasi segregasi transgresif hasil persilangan pada kacang hijau. Penelitian ini bertujuan untuk menduga nilai korelasi genotipe dan fenotipe antar sifat-sifat kuantitatif pada populasi segregasi transgresif kacang hijau. Bahan genetik adalah 11 galur segregan transgresif pada generasi seleksi S2:2 zuriat persilangan varietas Mamasa Lere Butnem × Lasafu Lere Butnem. Analisis korelasi genotipe diperoleh dari penguraian komponen ragam-peragam genetik dari hasil analisis peragam sesuai model acak rancangan acak lengkap dengan anak contoh, terdiri atas 13 genotipe (termasuk varietas penguji) yang diulang dua kali. Hasil penelitian memperlihatkan adanya korelasi genotipe antar sifat-sifat kuantitatif kacang hijau yang mengindikasikan kemungkinan seleksi langsung populasi galur segregasi transgresif terhadap hasil maupun tak-langsung melalui sifat yang mudah diamati seperti umur panen, jumlah polong dan jumlah biji. Kata Kunci: kacang hijau, korelasi genetik, sifat kuantitatif
Analisis Beta Karoten dan Vitamin pada Kulit dan Daging Buah Pisang Tongka Langit (Musa Troglodytarum L.) di Kota AmbonAROTEN DAN VITAMIN PADA KULIT DAN DAGING BUAH PISANG TONGKA LANGIT (Musa troglodytarum L.) DI KOTA AMBON Owend Paul Letelay; Adriana Hiariej; Anneke Pesik
Jurnal Agritechno Jurnal Agritechno, Vol. 13, Number 1, April 2020
Publisher : Depertemen Teknologi Pertanian Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.672 KB) | DOI: 10.20956/at.v13i1.243

Abstract

Banana Tongka Langit with fruit bunches soaring up has varied morphological characters including fruit size (long and short), fruit flesh color (yellow to orange) and different banana peel colors (yellow, yellowish orange, reddish orange, reddish brown, and brown) brownish orange) which indicates the presence of beta carotene content. The purpose of the study was to analyze the content of beta carotene and vitamins in the skin and flesh of the Tongka Langit banana using the High Performance Liquid Chromatography method. The results of the analysis of the highest beta carotene content in long fruit flesh is 65.33 mg / 100 and the lowest in short fruit meat is 22.31 mg / 100g. The most vitamin A found in short fruit skin 2260.63 mg / 100g and long fruit skin has the lowest value. The highest value of Vitamin B content is found in long fruit peels at 0.11 mg / 100g and the lowest in long fruit flesh and short fruit peels at 0.09 mg / 100g. The most vitamin C found in long fruit flesh is 28.48 mg / 100g, and the lowest in long fruit skin is 11.99 mg / 100g. The highest vitamin E was found in long fruit peels at 0.41 mg / 100g and the lowest was in short fruit flesh at 0.21 mg / 100g, while vitamin D was not found in all samples.
Epidermal Structure and Leaf Stomata of Several Accessions of Banana Plants (Musa spp.) Gebiana Nyainleta; Anneke Pesik; Adriana Hiariej
Jurnal Penelitian Pendidikan IPA Vol. 8 No. 6 (2022): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v8i6.2194

Abstract

This study aimed to determine the structure of epidermal cells and leaf stomata in Jarum, Merah, Tanduk, Kepok, and Pei or wild banana. The type of research used was quantitative to calculate the number of epidermis, epidermal cell size, number of stomata, and stomata index. Meanwhile, qualitatively described the structure of epidermal cells, epidermal cell shape, stomata shape, and stomata type of banana leaves based on the longitudinal incision method. The results showed that four accessions of bananas have epidermal cell shapes: rectangular, pentagon, hexagon, heptagon, octagonal, and regularly length. Meanwhile, Merah have different epidermal cell shapes, namely rectangular, pentagon, hexagon, and irregular, with untidy arrangements. The stomata of the five samples are surrounded by four to six subsidiary cells and are kidney-shaped. Based on the location of the leaf surface, stomata in five banana accessions have an amphistomatic type. Pei or wild bananas have a long epidermal size compared to the other four species but have a small number of epidermis. Then, the number of stomata in all banana species differs on the upper and lower surfaces. The number of stomata is more on the lower surface of the leaves. Stomata index in all banana species is low
GENETIC CHARACTERIZATION OF GALOBA DURIAN (AMONUM SPP.) IN AMBON ISLAND BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) Fuadiska Salamena; Adriana Hiariej; Cecilia Anna Seumahu
Agrotech Journal Vol 3, No 1 (2018): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v3i1.524

Abstract

Galoba durian is one of the endemic floras from Maluku. Galoba durian is a species belonging to the Amomum genera Zingiberaceae family. It is also used as a medicinal plant for waist and kidney diseases. Based on the color of the fruit, galoba durian is divided into two nmely red galoba durian and green galoba durian. Distribution of this plant in Ambon can found in a few places such as highland and coastal area. Different locations influence phenotypic of plants, but may not show different genetic characteristic. Genetic diversity can detected by molecular markers. Genetic characterization from galoba durian using RAPD markers has not been done before. This study aimed to analyze genetic diversity from galoba durian using molecular markers RAPD. Samples of plants are used red galoba durian from Hatu and green galoba durian from Hatalae. The result of the first study, characterization of the morphology of the galoba durian, showed that both galoba have almost similar characteristics. Further DNA was tested by qualitative and quantitative. Result shows good qualitative and quantitative of DNA genomic. The second study was amplification by PCR-RAPD. DNA amplifications were performed using 3 primers out of 9 screened random primers. The primers selection was based on hight polymorphism. DNA amplification has 36 bands which were 100% polymorphic. The size of each bands from visualization of agarose was determined by linear regression. Number of band amplified was range from 120 to 1612 bp. Polymorphic band of RAPD showed the highest  genetic diversity. It can be concluded that the two plants of galoba durian are different species
Genetic Characterization of Maize Kisar Var. Kuning Genjah and Maize Var. Bisi-II-Hibrida Based On Molekular RAPD Marker Elizabeth C. Berhitu; Adriana Hiariej; Cecilia A. Seumahu
Agrotech Journal Vol 4, No 2 (2019): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v4i2.1127

Abstract

The variety of maize in Southwest Maluku (MBD) has an ability to grow in the extremely dry land, especially at Kisar island, because those areas were dominated by dry land. Dry resistance very useful to be used for genetical characterization in order to provide accurate data about the character of local maize Kisar var. Kuning Genjah. To identify the characteristics at the genome level of this local maize variety, one of the molecular methods that can be applied was RAPD molecular method. The advantage of the RAPD marker is it's technically simpler and fast in testing, does not require DNA sequence information, hence this marker becomes widely used, it only requires a small sample of DNA, the primary is commercially available and does not use radioactive compounds. The aim of this study was to find out the bands profile that has the potential to be used as a marker of dry resistance. The method applied was RAPD (Random Amplified Polymorphic DNA) using OPA-02, OPA-07, and OPC-12 primers and produces 22 DNA bands. The measurement of bands by estimating the molecular weight based on marker exponential regression. The polymorphic percentage was 90.9% between var. Kuning genjah and var. BIZI-II-Hybrid. The percentage of polymorphism showed the potential of bands that can be used as molecular markers for markers of dry resistance that can be utilized in plant breeding
TRADITIONAL MEDICINE PLANTS OF WAAI VILLAGE COMMUNITIES, MALUKU PROVINCE Daro, Prichilia; Hiariej, Adriana; Nindatu, Maria
RUMPHIUS Vol 2 No 2 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i2p060-065

Abstract

This study aims to determine the types of plants used by the village community as traditional medicine and to find out how to use these types of medicinal plants. The research was conducted in Waai Village, Central Maluku District, Salahutu District. This research was conducted using survey methods and interviews with the community using a list of questions. The sampling technique was purposive sampling, the sample was determined as much as 15% of the total village population in Waai which consisted of 1500 heads of families and as many as 225 respondents were designated as heads of families. The results showed that there were 71 species of medicinal plants from 45 families. The most used plant parts are leaves, whole plants, fruit, roots, stems, rhizomes and tubers. Medicinal plants are generally used by the village community to cure ailments, aches and pains and to increase milk production. The utilization process is simple, by boiling, smoking, squeezing, rubbing and grating.
Co-Authors Aca A Anakotta Agus Sutanto Anneke Pesik Batlajery, Yulius Cecilia A. Seumahu CECILIA ANNA SEUMAHU Clemens Sudiyono Narahayaan Daro, Prichilia Dian Safitri Edizon Jambormias Elizabeth C. Berhitu FENNY MARTHA DWIVANY Fuadiska Salamena Gebiana Nyainleta Giasintha Stefani Husna Nugrahapraja I NYOMAN RAI Julian Timisela Katerina Jagagura Peilouw Ketut Wikantika Lamerkabel, Jacobus Suruka Aifaman Lapu, Petrus Martha Kaihena Masela, Efer Simon Nindatu, Maria Nisrina Sukriandi Owend Paul Letelay Patty, Kristi Lenci Pieter Agusthinus Riupassa Ridwan, La Rijal, Muhammad Riupassa, Pieter A. Rumpeniak, Yusuf Sahertian, Dece Elisabeth Sahubauwa, Laila Samputri, Salma Sulastri Sulastri Telussa, Ronald Topik Hidayat