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All Journal JFMR (Journal of Fisheries and Marine Research) Aquacultura Indonesiana
Gusti Ngurah Permana
Balai Besar Riset Perikanan Budidaya Laut Gondol
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Engineering Environmental Science

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DNA Profile of Pacific White Shrimp, L. vannamei Infected by Taura Syndrome Virus Using Single Strand Conformation Polymorphism (SSCP) Analysis Permana, Gusti Ngurah; Moria, Sari Budi; haryanti, haryanti
Aquacultura Indonesiana Vol 17, No 1 (2016)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.604 KB) | DOI: 10.21534/ai.v17i1.57

Abstract

The shrimp infected by Taura syndrome virus (TSV) can survive until harvesting time. At this phase, the growth of this shrimp is relatively normal but it is referred as TSV carrier. This research is expected to provide a recommendation in effort to prevent the occurrence of TSV infection. Therefore, this study was aimed to evaluate the DNA polymorhism of Pacific white shrimp L. vannamei infected by TSV. A total of 60 samples of L. vannamei were collected from SPF (specific pathogen free)-Florida, F1-healthy, F1-tolerance (it is can life normally after infected by TSV), and F1-moribund (diying after infected by TSV). The samples were analysis using PCR with IQ-2000 KIT to determine the positive infection of samples. DNA profile of white shrimp, L. vannamei was significantly different from SPF Florida, F1-moribund, and F1-healthy shrimp. This expression may control the defense mechanism. This can be ascribed to the reason that shrimp survived from TSV infection had a higher amount of locus (3 loci) that were detected from DNA polymorphism of gene in white shrimp contributed to a gene control that was determined from allele variation. Two important alleles (200 bp and 220 bp) unique may be associated with tolerant trait. Those loci only found in F1-tolerance shrimp. However to prove that further necessary to sequences a nucleotide of three loci as responsible for resistance to TSV infection.
“ULVA” PENGEMBANGAN TEKNIK PENANDA ALAMI PADA BUDIDAYA ABALON (Haliotis squamata) Permana, Gusti Ngurah; Rusdi, Ibnu; Wibawa, Gigih Satria; Setiabudi, Gede Iwan
JFMR (Journal of Fisheries and Marine Research) Vol. 5 No. 1 (2021): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2021.005.01.7

Abstract

"ULVA" NATURAL MARKING TECHNIQUES DEVELOPMENT IN ABALONE CULTURE (Haliotis squamata)  By  Gusti Ngurah Permana,, Ibnu Rusdia,  Gigih Satria Wibawaa and Iwan SetyabudibTagging is used to obtain some information during the process of selecting and managing population of abalone shells, Haliotis squamata. This research was conducted to determine the natural marker method of abalone, (H. squamata). The samples were used of  total length of the shell (initial average: 0.8 ± 0.2 mm).  Seed tagging using the Ulva sp. performed by giving a sequence of feed at the age of: 2-3 months (Ulva sp.), 3-4 months (Gracilaria sp.), 5-6 months (Ulva sp.), 6-10 months (Gracillaria sp.) and control 2 months (Ulva sp.), 3-10 months (Gracilaria sp.). The results of this study indicated that the tagging of the seeds with the Ulva method has a same growth pattern in both  treatment and control (38.93 ± 1.5 mm; 39.47 ± 1.44 mm). The effectiveness of Ulva tagging is seen in toca color finder (TC. 4103 green) and (TC 8135 brown), more sharpen in treatment compared to control. Ulva tagging technology can be applied to abalone seeds to see the sequence of the green color on the shell.  
Co-Authors Gede Iwan Setiabudi Haryanti Haryanti Ibnu Rusdi Sari Budi Moria Wibawa, Gigih Satria