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All Journal JFMR (Journal of Fisheries and Marine Research) Jurnal Riset Akuakultur Aquacultura Indonesiana
Gusti Ngurah Permana
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DNA Profile of Pacific White Shrimp, L. vannamei Infected by Taura Syndrome Virus Using Single Strand Conformation Polymorphism (SSCP) Analysis Permana, Gusti Ngurah; Moria, Sari Budi; haryanti, haryanti
Aquacultura Indonesiana Vol 17, No 1 (2016)
Publisher : Indonesian Aquaculture Society (MAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (472.604 KB) | DOI: 10.21534/ai.v17i1.57

Abstract

The shrimp infected by Taura syndrome virus (TSV) can survive until harvesting time. At this phase, the growth of this shrimp is relatively normal but it is referred as TSV carrier. This research is expected to provide a recommendation in effort to prevent the occurrence of TSV infection. Therefore, this study was aimed to evaluate the DNA polymorhism of Pacific white shrimp L. vannamei infected by TSV. A total of 60 samples of L. vannamei were collected from SPF (specific pathogen free)-Florida, F1-healthy, F1-tolerance (it is can life normally after infected by TSV), and F1-moribund (diying after infected by TSV). The samples were analysis using PCR with IQ-2000 KIT to determine the positive infection of samples. DNA profile of white shrimp, L. vannamei was significantly different from SPF Florida, F1-moribund, and F1-healthy shrimp. This expression may control the defense mechanism. This can be ascribed to the reason that shrimp survived from TSV infection had a higher amount of locus (3 loci) that were detected from DNA polymorphism of gene in white shrimp contributed to a gene control that was determined from allele variation. Two important alleles (200 bp and 220 bp) unique may be associated with tolerant trait. Those loci only found in F1-tolerance shrimp. However to prove that further necessary to sequences a nucleotide of three loci as responsible for resistance to TSV infection.
“ULVA” PENGEMBANGAN TEKNIK PENANDA ALAMI PADA BUDIDAYA ABALON (Haliotis squamata) Permana, Gusti Ngurah; Rusdi, Ibnu; Wibawa, Gigih Satria; Setiabudi, Gede Iwan
JFMR (Journal of Fisheries and Marine Research) Vol. 5 No. 1 (2021): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2021.005.01.7

Abstract

"ULVA" NATURAL MARKING TECHNIQUES DEVELOPMENT IN ABALONE CULTURE (Haliotis squamata)  By  Gusti Ngurah Permana,, Ibnu Rusdia,  Gigih Satria Wibawaa and Iwan SetyabudibTagging is used to obtain some information during the process of selecting and managing population of abalone shells, Haliotis squamata. This research was conducted to determine the natural marker method of abalone, (H. squamata). The samples were used of  total length of the shell (initial average: 0.8 ± 0.2 mm).  Seed tagging using the Ulva sp. performed by giving a sequence of feed at the age of: 2-3 months (Ulva sp.), 3-4 months (Gracilaria sp.), 5-6 months (Ulva sp.), 6-10 months (Gracillaria sp.) and control 2 months (Ulva sp.), 3-10 months (Gracilaria sp.). The results of this study indicated that the tagging of the seeds with the Ulva method has a same growth pattern in both  treatment and control (38.93 ± 1.5 mm; 39.47 ± 1.44 mm). The effectiveness of Ulva tagging is seen in toca color finder (TC. 4103 green) and (TC 8135 brown), more sharpen in treatment compared to control. Ulva tagging technology can be applied to abalone seeds to see the sequence of the green color on the shell.  
POLA PERTUMBUHAN DAN VARIASI GENETIK BERBASIS DNA MIKROSATELIT DARI TIGA POPULASI IKAN BARAMUNDI Lates calcarifer Khotimah, Fitriyah Husnul; Alimuddin, Alimuddin; Soelistyowati, Dinar Tri; Nuryati, Sri; Arfah, Harton; Sugama, Ketut; Permana, Gusti Ngurah; Sembiring, Sari Budi Moria; Haryanti, Haryanti
Jurnal Riset Akuakultur Vol 18, No 1 (2023): (Maret 2023)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.18.1.2023.49-59

Abstract

Benih ikan baramundi Lates calcarifer diperoleh dari pemijahan alami dengan jumlah induk terbatas sehingga variabilitas pertumbuhan dan kelangsungan hidup antar-batch menjadi tinggi. Penelitian dilakukan untuk menganalisis pertumbuhan dan mengevaluasi variasi genetik ikan kakap putih populasi Australia, Situbondo dan Lampung hasil domestikasi dan dibudidaya di hatcheri skala rumah tangga (HSRT). Sebanyak 10 ekor ikan barramundi dari setiap populasi digunakan untuk analisis variabilitas genetik dengan dua lokus mikrosatelit, yaitu Lca21 dan Lca32. Selanjutnya, data mikrosatelit diolah menggunakan software genetic analysis in excel (GenAlEx 6.51b2). Hasil yang diperoleh menunjukkan bahwa pertumbuhan panjang dan bobot tubuh serta laju pertumbuhan spesifik ikan baramundi dari Australia lebih tinggi dibandingkan dari Situbondo dan Lampung (P<0,05); sedangkan ikan baramundi dari Situbondo dengan Lampung adalah sama (P>0,05). Jumlah alel setiap lokus ikan baramundi berkisar 2-8 alel dan heterozigositas tertinggi dimiliki oleh ikan barramundi asal Situbondo (0,85), diikuti Lampung (0,65) dan paling rendah dari Australia (0,54). Dari hasil riset tersebut dapat disimpulkan bahwa ketiga populasi ikan baramundi hasil domestikasi dan dipeliharan dalam sistem HSRT memenuhi kelayakan untuk digunakan untuk kegiatan hibridisasi atau membentuk populasi awal (sintetis). Barramundi seeds, Lates calcarifer are usually sourced from natural spawning using a limited number of broodstock. Therefore, the growth and survival rate of these seeds vary greatly between batches. The research was performed to determine the growth pattern and genetic variations of barramundi seed populations produced from domesticated broodstock sourced from Australia, Situbondo, and Lampung and reared in small-scale hatcheries. Ten individuals of barramundi from each population were used for microsatellite analysis using two microsatellite loci, namely: Lca 21 and Lca 32. The resulted microsatellite data was processed using the genetic analysis available in Excel software (GenAlEx 6.51b2). The results showed that the growth in length and body weight as well as the specific growth rate of barramundi seeds produced from Australia broodstock were higher than that of Situbondo and Lampung (P<0.05) while the later two were similar (P>0.05). The number of microsatellite alleles ranged from 2-8 and the highest heterozygosity was obtained by barramundi seeds produced by Situbondo (0.85), followed by Lampung (0.65), dan Australia (0.54) broodstock. From the results of the research, it can be concluded that the three populations of barramundi fish, which were domesticated and reared in the HSRT system, meet the criteria for use in hybridization program or for forming a synthetic population.
Co-Authors Dinar Tri Soelistyowati Gede Iwan Setiabudi Harton Arfah Haryanti Haryanti Ibnu Rusdi Ketut Sugama Khotimah, Fitriyah Husnul Sari Budi Moria Sari Budi Moria Sembiring Sri Nuryati Wibawa, Gigih Satria