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All Journal Jurnal Sains dan Seni ITS IPTEK The Journal for Technology and Science Research Journal of Life Science Akta Kimia Indonesia Berkala Penelitian Hayati
Sri Nurhatika
Biology Departement, Faculty Of Science, Sepuluh Nopember Institute Of Technology, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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The Effect of Nitrogen Stress And Photoperiode on Growth and Fatty Acid Content of Nannochloropsis sp. Dini Ermavitalini; Sumarni Dwirejeki; Sri Nurhatika; Triono Bagus Saputro
Akta Kimia Indonesia Vol 4, No 1 (2019)
Publisher : LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (793.657 KB) | DOI: 10.12962/j25493736.v4i1.5089

Abstract

Energy consumption increases from year to year and 95% is fulfilled by fossil materials which are limited, so that a replacement energy source is needed. Biodiesel is a fuel from renewable sources such as plants or animals. Biodiesel from Jatropha curcas and palm oil has been produced but oxidation is low in cold temperatures and long time to production. On the other hand, Nannochloropsis sp., as marine microalgae can produce lipids as biodiesel material reaches 90% of the biomass dry weight in stress condition, It’s higher than lipid that produced by Jatropha curcas (35% DW) and palm (75,6% DW). Nannochloropsis sp. also short growth, easily propagated. Production of lipids and TAGs of  Nannochloropsis sp. can be engineered based on nutrient, temperature, aeration, salinity, photoperiod. This study aims to examine the effect of nitrogen stress and photoperiode on biomass, chlorophyll content, fatty acid profile, content of TAG. Nannochloropsis sp. cultured from the starter then cultured in variation of nitrogen stress and phoroperiode.  In the last exponential, culture analized in quantitative to get the biomass and chlorophyll content. Fatty acid and TAG analized by qualitative. The interaction between nitrogen and photoperiode did not affect the biomass of  Nannochloropsis sp. but it affects the chlorophyll content where the chlorophyll content of P14 (N100, 16: 8) is 7.367 mg/L. It is not significantly different from the treatment P6 (N25, 24: 0) of 3.339 mg/L and treatment P15 (N100, 24: 0) 5.665 mg/L. All cultures with a treatment combination showed triacylglycerol content. The results of the treatment of fatty acid profiles P14 (N100, 16: 8) contain more varied fatty acids compared to P1 (N0, 12:12), P2 (N0, 16: 8), P8 (N50, 62: 8), P10 (N75 , 12:12) which consists of lauric acid, hexagonal acidrionate, palmitic acid, linoleic acid, oleic acid, stearate acid, eicosanoic acid.The result showed that the treatment of nitrogen stress and photoperiode affecting the growth and fatty acid content of Nannochloropsis sp.
PEMBUATAN PUPUK BOKASHI DARI SAMPAH LINGKUNGAN BERDASARKAN RANCANGAN PERCOBAAN CAMPURAN YANG OPTIMUM PADA MODEL PERMUKAAN MULTIRESPON Ruslan; Susanti Linuwih; Purhadi; Sony Sunaryo; Sri Nurhatika
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.556 KB) | DOI: 10.23869/243

Abstract

Environment waste arround ITS college such as waste of Trembesi leaves, Sono leaves, and Satintail at this research will be used as part of materials of Bokashi fertilizer producing. The first aim of this research produced Bokashi fertilizer in various proportion waste of Trembesi leaves, Sono leaves, and Satintail with other materials proportion which has been assumed to be constant based on development of optimum mixture design of multiresponse surface theory by D-optimum criterion. The second aim of this research was compared 5:3:1:1 composition from reference to 5:2:1:2 composition from researcher hypothesis with proportion of waste of Trembesi leaves, Sono leaves, and Satintail as independent variables to N, P, and K percentation as response variables. Optimum mixture designs of multiresponse surface model will be optimum if it weighted w = 1 / qs means the each point of experiment will be given s-replications as much as proportion number of experiment point to number of component. The 5:2:1:2 and 5:3:1:1 compositions of Bokashi fertilizer producing are not statistically difference, therefore both compositions can be used to produce Bokashi fertilizer.
Co-Authors Anton Muhibbudin Anton Muhibuddin Anton Muhibuddin Anton Muhibuddin Astrid Rizka Raysendi Ayik Sanggilora Chantika Amelia Arisandi Chintya Putri Dini Ermavitalini Dyah Agustina Ella Ratih Wahyu Indira Rizqita Ivanesthi Indira Rizqita Ivanesthi Kristanti Indah Purwani Lailatus Saidah Lisma Shofarina Purwati Maretika Wulan Syafitri Sasmita Muhibuddin, Anton Mutiara Arum Kusumaningati Naufa Munadiyal Mustaqimah Ni Kadek Marina Dwi Cahyani Nurhayati, Awik P.D. Purhadi Purhadi Ruslan Sabrina Firrar Rahmana Sevy Dwi Kartikasari Siti Nurhalimah Sony Sunaryo Sumarni Dwirejeki Susanti Linuwih Tjuk Eko Hari Basuki Triono Bagus Saputro, Triono Bagus Vinka Adetya Wahyu Rizqi Wicaksono