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PENGARUH SITOTOKSIK EKSTRAK BUAH MAHKOTADEWA [Phaleria macrocarpa (Scheff) Boerl.] TERHADAP SEL KANKER LESTARI HeLA Pertamawati, Pertamawati
Jurnal Sains dan Teknologi Indonesia Vol. 9 No. 1 (2007)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (51.521 KB) | DOI: 10.29122/jsti.v9i1.769

Abstract

Fruits of mahkotadewa (Phaleria macrocarpa (Scheff) Boerl). has been used to cure various health problems, including empirical treatment for cancer. The therapeutic effect of natural material is related to the chemical compounds it contain. In fruits of Phaleria macrocarpa, various chemicals are present such as flavonoid and tannin, both show anticancer activity, as well as other unknown ones presumably supportive for cancer treatment. An in vitro experiment is therefore set up with the objective of examining the effect of fruit extract on human uterine cervical carcinoma cells (HeLa cells). Various concentrations: 5, 10, 25, 50 and 100 ppm of fruit extract were evaluated. Observations were made 24, 72 and 120 hour after incubating HeLa cells in each treatments. The results showed that fruit might inhibit the growth of HeLa cells. Inhibitory concentration (IC50) of fruit extract after 24 hours of incubation is 6,21 ppm, after 72 hour of incubation is 5,09 ppm dan 5,73 ppm for 120 hour of incubation. Inhibitory potential of fruits in 72 hour of incubation is a highest ones. The effect might be associated to the active compounds contained in these material.
PENGARUH FOTOSINTESIS TERHADAP PERTUMBUHAN TANAMAN KENTANG (SOLANUM TUBEROSUM L.) DALAM LINGKUNGAN FOTOAUTOTROF SECARA INVITRO Pertamawati, Pertamawati
Jurnal Sains dan Teknologi Indonesia Vol. 12 No. 1 (2010)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (68.411 KB) | DOI: 10.29122/jsti.v12i1.848

Abstract

The growth responces of potato explant (var. Atlantic), under different temperatures (100C) between photoperiod and dark period air temperatures in culture room, in high intensity of light (7000 lux) and low intensity of light (3000 lux), cultured in 18 h light/dark cycle and incubated for 21 and 28 days wasobserved. The result shown that photoautotroph condition influence the explant growth to be planlet. After 28 days incubation the planlets growth better than in 21 days. Its because the photosynthesis process during 28 days incubation were more effective than in 21 days incubation. The dry weight per planlet, number of leaves, leaf area and dry weight per leaf were enhanced in photoautotroph condition with 7000 lux light intensity in all treatments. In 3000 lux light intensity, the high planlet with small diameter was performanced. The study indicates that the photoautotroph condition affect further growth of the in vitro potato planlet.
Konsumsi Ekstrak Secang (Caesalpinia sappan L.) Terhadap Volume Urin Tikus Putih Jantan Galur Spraque Dawley Pertamawati, Pertamawati; Sriningsih, Sriningsih; Fahrudin, Fahri; Efendi, Julham
Jurnal Jamu Indonesia Vol. 2 No. 3 (2017): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v2i3.41

Abstract

Secara empiris secang (Caesalpinia sappan L.) dimanfaatkan sebagai bahan untuk mengatasi penyakit, namun bagaimana pengaruhnya terhadap pengeluaran urin belum diketahui. Penelitian ini bertujuan untuk mengetahui pengaruh konsumsi ekstrak secang terhadap pengeluaran urin. Percobaan dilakukan terhadap hewan coba tikus putih jantan galur Spraque dawley dengan sampel uji ekstrak etanol 96% kulit kayu secang. Hewan coba dibagi dalam 7 kelompok, masing-masing terdiri dari 6 ekor, yaitu kelompok normal, kelompok positif (Furosemide 10mg/200g BB), kelompok negatif (air 2ml/200g BB), kelompok sampel uji dosis I (62.5 mg/kg BB), dosis II (125mg/kg BB), dosis III (250mg/kg BB) dan dosis IV (500mg/kg BB), tigapuluh menit setelah pemberian sampel uji, hewan coba diberi air minum 4ml/200g BB dilakukan melalui sonde lambung, selanjutnya hewan coba dimasukkan ke dalam kandang metabolit selama 16 jam (overnight). Hewan coba dengan dosis II menghasilkan volume urin paling besar (8.00 mL) yaitu hampir dua kali lipat dibandingkan urin yang dihasilkan oleh kelompok normal (4.50 mL) Volume urin berkurang jika konsumsi ekstrak secang bertambah pada dosis III dan IV. Disimpulkan bahwa hewan coba yang diberi ekstrak kulit secang dengan dosis 125 mg/kg BB (dosis II) rata-rata mampu mengeluarkan urin lebih banyak daripada hewan coba dalam kelompok lainnya.
Opportunities to Elevate Levels of Curcumin in Curcuma xanthorrhiza Plants Pertamawati, Pertamawati
Jurnal Jamu Indonesia Vol. 3 No. 1 (2018): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v3i1.44

Abstract

Production of curcuminoids from Curcuma xanthorrhiza using conventional cultivation technique requires a very long time from planting to harvesting process. The use of biotechnology known as the callus culture is expected to help this problem. An experiment was conducted with the explant of curcuma leaves which are cultured in basic media of Murashige & Skoog (MS) with addition of growing substances Phenilalanine (2-amino-3-fenil-propanoat acid/C9H11NO2) with a concentration of 2, 4 and 8 mg/L and Sodium Acetate (CH3COONa) with concentration of 0.2 and 4 mg/L. Experiments done with random design with 3 times replication. The results showed that addition of Phenilalanine at a concentration of 4 mg/L produced curcuminoids namely curcumin 0.8861% and desmethoxycurcumin 0.3307%, while the addition of sodium acetate 2 mg/L was able to induce the formation of curcuminoids namely curcumin 0.7514% and desmethoxycurcumin 0.3898%. It is suggested that further research with a callus induction technology in tissue culture need to be conducted for producing curcumin in an industrial scale.