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All Journal Jurnal Natur Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI
Yuyu Suryasari Poerba
Pusat Penelitian Biologi-LIPI, Cibinong Science Centre, Jl. Raya Bogor Km. 46, Cibinong 16911
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Veterinary

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Identifikasi Genetik Mutan Talinum paniculatum JACQ. (GAERTN.) Berdasarkan Marka RAPD Poerba, Yuyu Suryasari
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.61 KB) | DOI: 10.31258/jnat.12.1.44-48

Abstract

Javasom Talinum paniculatum Jacq. (Gaertn.) is one of popular vegetable plants with potential medicinal properties.The plant root is often used as a substitute for ginseng (Panax ginseng L.), and the leaves are used as a vegetableand as a substitute for purslane (Portulaca olaraeae L.). An effort to improve genetic quality of the plant was madethrough induced mutation with ethyl methane sulphonate (EMS). A variety of EMS dosages (0, 0.3%, 0.6%, 0.9%,1.2%, 1.5% dan 1.8%) were applied to javasom seeds to induce mutation for 24 hours at room temperature. Selectedfive putative mutant genotypes of the third generation after mutagen treatment (M3) was analyzed using Ramdomamplified polymorphic DNA (RAD) marker. Twenty RAPD primers generated 185 scorable bands with 78.92% ofthem were polymorphic. Size of the bands varied from 200bp to 2.5kbp. Clustering analysis was performed basedon RAPD profiles using the UPGMA method. The range of genetic distance among individual genotypes was fromfrom 0.31 to 0.58, while genetic variance was 0.29 + 0.17. The five genotypes were proof to be solid mutants.
Induksi Mutasi Curcuma zedoaria (Christm) Roscoe dengan Irradiasi Sinar Gamma Poerba, Yuyu Suryasari; Martanti, Diah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.515 KB) | DOI: 10.24002/biota.v14i2.2686

Abstract

Curcuma zedoaria (Zingiberaceae) is one of Curcuma spp. used as stomachic,carminative, haematic and styptic medicines. Dried rhizomes of C. zedoaria containessential oil and curcuminoids. Induced mutation of this species would provide andhelp plant breeders to improve and select better quality of the plant. The presentstudy was carried out to determine LD-50% of gamma irradiation of C. zedoaria, toinduce genetic variation using gamma irradiation, and to assess genetic diversityamong mutant genotypes using random amplified polymorphic DNA (RAPD)markers. The LD-50% of gamma irradiation was achieved at 813.32 rad. Chlorophyllmutations were recorded at vM1 and vM2. Result of genetic analysis showed thatRAPD profiles were different from that of the control, and genetic dissimilaritiesamong mutant genotypes were higher than that of the control.
Genetic Variation of Wild Musa acuminata Colla From Indonesia Poerba, Yuyu Suryasari; Martanti, Diyah; Ahmad, Fajarudin
BIOTROPIA Vol. 26 No. 2 (2019): BIOTROPIA Vol. 26 No. 2 August 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (965.188 KB) | DOI: 10.11598/btb.2019.26.2.896

Abstract

Indonesia is the center of origin and diversity of Musa acuminata Colla, one of the progenitors of cultivated bananas today. However, the genetic variation of wild M. acuminata has not been studied extensively. Therefore, this study aimed to assess the genetic variation of Indonesian wild M. acuminata based on 10 RAPD (Random Amplified Polymorphic DNA) and 10 ISSR (Inter Simple Sequence Repeats) markers. The genetic properties of 17 populations of wild M. acuminata were analyzed using PopGene 1.32 based on Nei’s unbiased measures of genetic identity and genetic distance. Of the 443 DNA bands produced, 425 (95.94%) were polymorphic. Cluster analysis of the combined RAPD and ISSR data produced a dendrogram that separated the population of M. acuminata (A genome) from M. balbisiana (B genome), but not from M. schizocarpa (S genome). Nei’s genetic distance among the 17 populations of wild M. acuminata ranged from 0.03676 to 0.1634. The highest genetic distance was observed between M. acuminata var. rutilifes (from East Java) and M. acuminata var. sumatrana (from West Sumatra). The percentage of polymorphic loci among the 17 populations of M. acuminata ranged from 9.93% to 39.73%. Nei’s gene diversity (h) ranged from 0.0410 to 0.1418. The population of M. acuminata var. malaccensis displayed the highest genetic diversity among the 17 evaluated populations. The high level of genetic diversity of the wild M. acuminata from Indonesia highlights the importance of conservation and preservation of natural populations, as well as their potential use in banana breeding programs.
Co-Authors Ahmad, Fajaruddin Diyah Martanti, Diyah Erlin Rachman FAJARUDIN AHMAD Fauzia Syarif Handayani, T Herlina Herlina Imelda, Maria Martanti, Diah Widjaya, Elizabeth A. Witjaksono Witjaksono