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All Journal Jurnal Fitopatologi Indonesia JURNAL TEKNOLOGI LINGKUNGAN Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA
FAJARUDIN AHMAD
Department of Biology, Faculty of Mathematic and Natural Science, Bogor Agricultural University, Darmaga Campus, Bogor 16680, Indonesia Research Center for Biology, Indonesia Institute of Science (LIPI), Jalan Raya Jakarta Bogor Km. 46, Cibinong 16
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Medicine & Pharmacology

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Persilangan Pisang Liar Diploid Musa acuminata Colla var malaccensis (RIDL.) Nasution Sebagai Sumber Polen dengan Pisang Madu Tetraploid Poerba, Yuyu S.; Ahmad, Fajarudin; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.794 KB) | DOI: 10.14203/jbi.v8i1.3076

Abstract

Hybridization of wild diploid Musa acuminata Colla var malaccensis (Ridl.) Nasution as pollen source with teraploid Pisang Madu. Indonesia and South East Asia is the center of origin and genetic variability of bananas, specifically species Musa acuminata Colla. At least 15 varieties of wild Musa acuminata are found in Indonesia. Some of them have resistances for several diseases of bananas. One approach in banana breeding program is hybridizingbetween tetraploid parent and diploid parent of banana. This research was aimed to produce new triploid banana hybrid by crosses between tetraploid female parent ‘Pisang Madu’ and wild diploid male parent Musa acuminata Colla var malaccensis (Ridl.) Nasution. From 302 crosses, 53.9% of crosses produced seeds. However, only 48.46% of seeds contained embryos, and only 1.27% of embryos grew and developed. Bunch weight and leaf length of hybridplants were not significantly different with female parent, tetraploid Pisang Madu. Plant height of the hybrids was not significantly different with male parent, wild diploid Musa acuminatavar malaccensis. Pseudostem diameter and leaf width of the hybrid plants were between female tetraploid parent and male wild diploid parent. RAPD profiles (DNA bands) of the hybrids were originated from the female parent, male parent and both parents were 38.46, 34.6, and 26.92%, respectively. The hybrids were confirmed to be triploids (3x=33).Keywords: banana, breeding, hybrids, tetraploid, wild diploid, Pisang Madu, Musa acuminata Colla var malaccensis (Ridl.) Nasution
ANALISIS KERAGAMAN GENETIK Musa balbisiana Colla BERDASARKAN MARKA RAPD DAN ISSR [Genetic variation analyses of Musa balbisiana Colla based on RAPD and ISSR markers] Poerba, Yuyu Suryasari; Ahmad, Fajarudin
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (529.81 KB) | DOI: 10.14203/beritabiologi.v12i2.540

Abstract

Wild Musa balbisiana Colla is one of the progenitors of cultivated bananas and plantains. It is originated in Asia, and distributed from India to Papua New Guinea. This study was conducted to assess the molecular diversity of 25 accessions of M. balbisiana based on Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) analyses. RAPD and ISSR fingerprints of these banana varieties were detected by amplifications of nine primers of RAPDs and six primers of ISSRs. RAPD primers produced 84 amplified fragments varying from 150 bp to 2300 bp in size. 21.43 % of the amplification bands were polymorphic. ISSR primers produced 61 amplified fragments varying from 250 bp to 2200 bp in size. 29.30 % of the amplification bands were polymorphic. Based on these results, the 25 accessions of Indonesian M. balbisiana showed a low genetic variation, with coefficient of similarity ranged from 0.81 to 0.99.
Keragaman Genetik Kultivar Pisang Diploid (AA) Koleksi Cibinong Science Center Berdasarkan Marka RAPD dan ISSR Poerba, Yuyu Suryasari; Ahmad, Fajarudin
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (471.587 KB) | DOI: 10.24002/biota.v15i3.2584

Abstract

The banana (Musa acuminata Colla) is considered as an important crop plant due to its high economic value which also has good dietary source. Here, the genetic variation of 20 diploid (AA) banana cultivars from Cibinong Science Center collection were analyzed. Random amplified polymorphic DNAs (RAPDs) and Inter Simple Sequence Repeats fingerprinting of these banana cultivars were carried out by four primers of RPDSs and two primers of inter simple sequence repeats (ISSRs) led to DNA amplification. The amplification products of RPADs and ISSRs were polymorphic, 97.83% and 95%, respectively. Size of the bands was varied from 350bp to 2.0 kbp. The range of genetic distance was from 0.06 to 0.07. The molecular data showed that these banana varieties were diverse collection.
ANALISIS KERAGAMAN GENETIK KENTANG HITAM Yulita, Kusumadewi Sri; Ahmad, Fajarudin; Martanti, Diyah; Poerba, Yuyu S.; Herlina, Herlina
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.686

Abstract

Kentang hitam [Plectranthus rotundifolius (Poiret) Sprengel] of family Lamiaceae is a minor root crop known only for people living in some parts of Java, Bali and Madura. It was rarely found in its natural habitat, thus it was assumed to have low level of genetic diversity. This present study aimed to assess genetic diversity of 63 accessions of kentang hitam from provenances of Java based on Inter Simple Sequence Repeats (ISSR) and Random Amplified Polymorphic DNA (RAPD) fingerprints. Ten primers of ISSR and RAPD were initially screened and eight were selected for the analysis. These eight primers (OPA13, OPB10, OPB13, OPD8, OPN14, UBC 807, 834 and 835) generated 61 bands with an average of 7.63 polymorphic fragment per primer. Percentage of polymorphism ranged from 8.20% (UBC 807 and 834) to 16.39% (OPB 10) with an average of 12.50%polymorphism.Clustering analysis was performed based on ISSR and RAPD profiles using the neighbour joining method and Principle Coordinate Analysis (PCO). The range of genetic similarity among accessions was 51-100% to which most of the accessions were clustered with more than 80% similarity. This confirmed our hypothesis of the low level of variation existed among accessions.
PENAMPILAN RANDOM AMPLIFIED POLYMORPHIC DNA PADA Azadirachta indica A. Juss DARI TAMAN NASIONAL BALURAN Ahmad, Fajarudin; Poerba, Yuyu S.
Jurnal Teknologi Lingkungan Vol. 11 No. 1 (2010)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.526 KB) | DOI: 10.29122/jtl.v11i1.1223

Abstract

Azadirachta indica A. Juss (Apocynaceae) is a large tree of the lowland tropical rain forest of Southeast Asia that occurs in Thailand, the Malay Peninsula, on the island of Java (East Java) and Lesser Sunda Islands. Its economic value was in its wood (timber), and as medicinal plant. The information on genetic diversity of the species is very limited. Hence studies were initiated and genetic diversity estimated using RAPD markers in 27 accessions of A.indica procured from three geographical regions of TN Baluran and Balai Litbang Kehutanan. Seven selected Operon primers (10 mer) generated a total of 133 consistent amplification products ranging from 132 bp to 5.6 Kb. The cluster analysis separated the 27 individuals into 2 clusters. The range of genetic dissimilarityvalue among samples was from 0.07 to 0.33, while genetic distance among populations was from 0.04 to 0.10. These values showed that A. indica from TN Baluran was not genetically diverse population. Key words: Azadirachta indica, genetic diversity, RAPD
PERSILANGAN PISANG LIAR DIPLOID MUSA ACUMINATA COLLA VAR MALACCENSIS (RIDL.) NASUTION SEBAGAI SUMBER POLEN DENGAN PISANG MADU TETRAPLOID Poerba, Yuyu S.; Ahmad, Fajarudin; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3076

Abstract

Hybridization of wild diploid Musa acuminata Colla var malaccensis (Ridl.) Nasution as pollen source with teraploid Pisang Madu. Indonesia and South East Asia is the center of origin and genetic variability of bananas, specifically species Musa acuminata Colla. At least 15 varieties of wild Musa acuminata are found in Indonesia. Some of them have resistances for several diseases of bananas. One approach in banana breeding program is hybridizingbetween tetraploid parent and diploid parent of banana. This research was aimed to produce new triploid banana hybrid by crosses between tetraploid female parent ?Pisang Madu? and wild diploid male parent Musa acuminata Colla var malaccensis (Ridl.) Nasution. From 302 crosses, 53.9% of crosses produced seeds. However, only 48.46% of seeds contained embryos, and only 1.27% of embryos grew and developed. Bunch weight and leaf length of hybridplants were not significantly different with female parent, tetraploid Pisang Madu. Plant height of the hybrids was not significantly different with male parent, wild diploid Musa acuminatavar malaccensis. Pseudostem diameter and leaf width of the hybrid plants were between female tetraploid parent and male wild diploid parent. RAPD profiles (DNA bands) of the hybrids were originated from the female parent, male parent and both parents were 38.46, 34.6, and 26.92%, respectively. The hybrids were confirmed to be triploids (3x=33).Keywords: banana, breeding, hybrids, tetraploid, wild diploid, Pisang Madu, Musa acuminata Colla var malaccensis (Ridl.) Nasution
Detection of Fusarium wilt on Local Bananas in Pandeglang Nani, Maryani; Harahap, Elmira Oktaria Raihan; Khastini, Rida Oktorida; Ahmad, Fajarudin
Jurnal Fitopatologi Indonesia Vol 19 No 4 (2023): Juli 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.4.133-144

Abstract

Pandemi layu fusarium pisang atau Panama disease, yang disebabkan oleh tropical race 4 (TR4), menjadi ancaman budi daya pisang baik industri maupun skala kecil. Di Indonesia, layu fusarium menyerang varietas ekspor pisang Cavendish dan varietas lokal populer seperti Pisang Raja, Kepok, dan Barangan. Meskipun demikian, TR4 masih belum banyak diketahui keberadaannya pada pisang-pisang lokal di berbagai wilayah Indonesia. Penelitian ini bertujuan mendeteksi TR4 pada pisang-pisang lokal Pandeglang, yang merupakan wilayah terbesar penghasil pisang di Provinsi Banten. Survey dilakukan di sembilan lokasi yang tersebar di lima kecamatan: Banjar, Cadasari, Jiput, Labuan, dan Menes. Total 13 aksesi pisang lokal menunjukkan gejala internal dan eksternal layu fusarium. Sampel pseudostem bergejala ditumbuhkan pada medium agar-agar dekstrosa kentang. Kultur spora tunggal digunakan untuk identifikasi dan isolasi DNA. Berdasarkan karakter mikroskopisnya, 27 galur Fusarium yang terdiri atas 3 komplek species berhasil diidentifikasi yaitu F. cugenangense, F. foetens dan F. oxysporum (Fusarium oxysporum species complex; FOSC), F. equiseti dan F. incarnatum (Fusarium incarnatum-equiseti species complex) dan F. solani (Fusarium solani species complex atau Neocosmospora). Deteksi molekuler duplex PCR menggunakan primer translation elongation factor-1α (Tef-1α) dan primer specific TR4, memberikan hasil positif hanya pada galur-galur yang merupakan TR4, anggota dari FOSC. Penelitian ini memperkaya pengetahuan keberadan TR4 pada daerah yang belum teridentifikasi dan potensi ancaman TR4 yang menyerang pisang-pisang lokal di Pandeglang. Analisis molekuler lebih lanjut menggunakan sikuen gen penanda atau whole genome sequencing dapat memberikan pengetahuan akan diversitas genetik Fusarium asal Pandeglang sehingga metode penanganan yang tepat dapat dilakukan.
Co-Authors Diyah Martanti, Diyah Harahap, Elmira Oktaria Raihan Herlina Herlina KUSUMADEWI SRI YULITA Nani, Maryani Rida Oktorida Khastini Witjaksono Witjaksono Yuyu S. Poerba, Yuyu S. Yuyu Suryasari Poerba