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All Journal Microbiology Indonesia Jurnal Kimia Terapan Indonesia Bulletin of Chemical Reaction Engineering & Catalysis

Hardaning Pranamuda

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Author-ID : 344669

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Articles

Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil YUDHIE ISTIANTO; RADEN SETYO ADJI KOESOEMOWIDODO; YOSHIO WATANABE; HARDANING PRANAMUDA; BAMBANG MARWOTO
Microbiology Indonesia Vol. 6 No. 1 (2012): March 2012
Publisher : Indonesian Society for microbiology

Phenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) phenol solution at 30 oC for 30 minutes, and subsequently cultured on plates of humic acid-vitamin agar (HVA) medium supplemented with cycloheximide (50 μg/mL) and nystatin (50 μg/mL). A total of 61 isolates were obtained and the most dominant isolates were not Streptomyces (only 24.6%), whereas other genera such as Micromonospora, Actinomadura, Microbispora and Polymorphospora were isolated with ratios of 49.2%, 13.1%, 9.8%, and 3.3%, respectively.

SINTESIS POLI (GLIKOLIDA-CO-LAKTIDA) DAN UJI BIODEGRADABILITASNYA Rofiq Sunaryanto; Hardaning Pranamuda
Jurnal Kimia Terapan Indonesia Vol 17, No 2 (2015)
Publisher : Research Center for Chemistry - LIPI

Polylactide and polyglycolide are important aliphatic polyester consist of lactide and glycolide repeats unit. It is considered as green polymer due to it is produced from renewable agricultural resources. The development of biodegradable plastics based on agricultural resources is still important to be done. Copolymerization of glycolide with D-lactide, L-lactide, and DL-lactide using a Sn(Oct)2 as catalyst had been carried out. Polymerization was conducted by ring-opening of lactide and glycolide without the solvents but melting polymerization. Polymerization were conducted at 130 Â°C for 130 hours. Result of this study showed that polymerization of glycolide with D-lactide produce polymers with an average molecular weight of 8.2x103 and a yield of 72 %. Polymerization of glycolide with L-lactide produce polymer with an average molecular weight of 2.8x103 and a yield of 36 %. Polymerization glycolide with DL-lactide produce polymer with an average molecular weight of 2.5x103 with a 48 % yield. The burial test for 28 days showed poly (glycolide-co-Llaktida) degraded faster than other polymers, namely degraded 32 %. While poly (glycolide-co-DL-lactide) and poly (glycolide-co-D-lactide) showed similar degradation that is equal to 20 %. Compared with PHB (polyhydroxybutyrate), poly (glycolide-co-lactide) degraded more slowly.Keywords: Glycolide, lactide, copolymerization, biodegradable plastic

Isolation of Oxo-degradable Polyethylene Degrading-Bacteria of Benowo Landfill Soil Surabaya MUKAMTO MUKAMTO; YUNI SRI RAHAYU; LISA LISDIANA; HARDANING PRANAMUDA
Microbiology Indonesia Vol. 9 No. 1 (2015): March 2015
Publisher : Indonesian Society for microbiology

The widespread consumption of oxo-degradable polyethylene plastics in Indonesia is potentially cause pollution. This problem can be overcome by utilize plastic degrading-bacteria as degradation agent. The present study aims to isolate and characterize of oxo-degradable polyethylene degrading-bacteria from Benowo landfill soil Surabaya. Isolation and screening was done by the culture technique and clear zone method. Total abundance of bacteria was calculated based on the Total Plate Count method. Bacterial colonies screening was done based on morphological characteristics and the diameter of clear zone. Four isolates with the largest diameter of clear zone were futher characterized based on cell morphology and physiology biochemistry characters. The results showed the average of total abundance of oxo-degradable polyethylene degrading-bacteria of Benowo landfill was 1.28 x 104 CFU g-1. The four of twelve isolates with the largest diameter of clear zone was indicated the highest degradation capability, that were isolates A221 (0.7 cm), A231 (1 cm), A232 (0.6 cm), and C231 (1.3 cm). Colony morphology characteristics of four isolates were generally shape of circular and irregular; margin of entire and lobate; elevation of flat; optics of opaque; and pigmentation of yellow, greenish and cream. These four isolates were Gram negative with the shape bacilli and cell size range of 3-4 Î¼m. Physiological and biochemical characteristics among the four isolates were resistant to acidic conditions; non-motile; produce catalase enzyme except A231; capable to reduce glucose and mannitol; uncapable to reduce lactose; capable to produce acids, except A232; produce of 2.3-butanediol; unable reduce sodium malonate; and capable to reduce nitrate, except A232 and C231; as well as producing hydrogen sulfide except A221 and A232. The four isolates has similar properties with the genus Mycobacterium.

Modification of HZSM-5 with Phosphotungstate, Silver, and Cobalt to Enhance Catalytic Reaction of Bioethanol to Bioethylene Risnawati, Dhea Atika; Pradana, Nova Yoga; Rochmadi, Rochmadi; Prasetyo, Imam; Saputra, Daffa Dewa; Pranamuda, Hardaning; Tandio, Sugianto; Ariyanto, Teguh
Bulletin of Chemical Reaction Engineering & Catalysis 2026: BCREC Volume 21 Issue 2 Year 2026 (August 2026)
Publisher : Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.9767/bcrec.20604

Developing an optimal catalyst formulation is a critical challenge in expanding sustainable ethylene production and utilization as a chemical intermediate product. Metal oxide impregnation (silver nitrate (AgNO3), cobalt nitrate (Co(NO3)2), and phosphotungstic acid (H3PW12O40)) was used to enhance the catalytic performance of HZSM-5 by increasing active sites and acidity. The preparation of the catalyst was performed by the impregnation of various metals and amounts of loading particles inside the pores of HZSM-5. The particles were impregnated by incipient wetness impregnation and followed by calcination to obtain Ag/HZSM-5, Co/HZSM-5, and W/HZSM-5 catalysts. Characterization techniques, including N2 adsorption-desorption, SEM, and XRD, were used to analyze the catalyst properties. Catalytic performance was evaluated in a packed-bed reactor under varying reaction conditions at WHSV 1.8 h-1. The aim of this research is to identify optimal catalyst formulations that exhibit superior activity in both conversion and selectivity towards ethylene production. Modified HZSM-5 catalysts incorporating Ag, Co, and W exhibited enhanced catalytic performance for bioethanol dehydration to bioethylene, attributed to optimized acidic sites, pore structure, and metal synergy. The 1%W/HZSM-5 catalyst demonstrated superior ethylene conversion (98.2%) and selectivity (99.88%) at 300 ℃. Increasing tungsten loading up to 2% impacted the conversion of bioethanol. Copyright © 2026 by Authors, Published by BCREC Publishing Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).

Co-Authors BAMBANG MARWOTO Imam Prasetyo Lisa Lisdiana MUKAMTO MUKAMTO Pradana, Nova Yoga RADEN SETYO ADJI KOESOEMOWIDODO Risnawati, Dhea Atika Rochmadi Rochmadi Rofiq Sunaryanto Saputra, Daffa Dewa Tandio, Sugianto Teguh Ariyanto YOSHIO WATANABE YUDHIE ISTIANTO YUNI SRI RAHAYU

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