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All Journal Microbiology Indonesia Jurnal Kimia Terapan Indonesia Bulletin of Chemical Reaction Engineering & Catalysis

Hardaning Pranamuda

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Author-ID : 344669

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil YUDHIE ISTIANTO; RADEN SETYO ADJI KOESOEMOWIDODO; YOSHIO WATANABE; HARDANING PRANAMUDA; BAMBANG MARWOTO
Microbiology Indonesia Vol. 6 No. 1 (2012): March 2012
Publisher : Indonesian Society for microbiology

Phenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) phenol solution at 30 oC for 30 minutes, and subsequently cultured on plates of humic acid-vitamin agar (HVA) medium supplemented with cycloheximide (50 μg/mL) and nystatin (50 μg/mL). A total of 61 isolates were obtained and the most dominant isolates were not Streptomyces (only 24.6%), whereas other genera such as Micromonospora, Actinomadura, Microbispora and Polymorphospora were isolated with ratios of 49.2%, 13.1%, 9.8%, and 3.3%, respectively.

Isolation of Oxo-degradable Polyethylene Degrading-Bacteria of Benowo Landfill Soil Surabaya MUKAMTO MUKAMTO; YUNI SRI RAHAYU; LISA LISDIANA; HARDANING PRANAMUDA
Microbiology Indonesia Vol. 9 No. 1 (2015): March 2015
Publisher : Indonesian Society for microbiology

The widespread consumption of oxo-degradable polyethylene plastics in Indonesia is potentially cause pollution. This problem can be overcome by utilize plastic degrading-bacteria as degradation agent. The present study aims to isolate and characterize of oxo-degradable polyethylene degrading-bacteria from Benowo landfill soil Surabaya. Isolation and screening was done by the culture technique and clear zone method. Total abundance of bacteria was calculated based on the Total Plate Count method. Bacterial colonies screening was done based on morphological characteristics and the diameter of clear zone. Four isolates with the largest diameter of clear zone were futher characterized based on cell morphology and physiology biochemistry characters. The results showed the average of total abundance of oxo-degradable polyethylene degrading-bacteria of Benowo landfill was 1.28 x 104 CFU g-1. The four of twelve isolates with the largest diameter of clear zone was indicated the highest degradation capability, that were isolates A221 (0.7 cm), A231 (1 cm), A232 (0.6 cm), and C231 (1.3 cm). Colony morphology characteristics of four isolates were generally shape of circular and irregular; margin of entire and lobate; elevation of flat; optics of opaque; and pigmentation of yellow, greenish and cream. These four isolates were Gram negative with the shape bacilli and cell size range of 3-4 Î¼m. Physiological and biochemical characteristics among the four isolates were resistant to acidic conditions; non-motile; produce catalase enzyme except A231; capable to reduce glucose and mannitol; uncapable to reduce lactose; capable to produce acids, except A232; produce of 2.3-butanediol; unable reduce sodium malonate; and capable to reduce nitrate, except A232 and C231; as well as producing hydrogen sulfide except A221 and A232. The four isolates has similar properties with the genus Mycobacterium.

Co-Authors BAMBANG MARWOTO Imam Prasetyo Lisa Lisdiana MUKAMTO MUKAMTO Pradana, Nova Yoga RADEN SETYO ADJI KOESOEMOWIDODO Risnawati, Dhea Atika Rochmadi Rochmadi Rofiq Sunaryanto Saputra, Daffa Dewa Tandio, Sugianto Teguh Ariyanto YOSHIO WATANABE YUDHIE ISTIANTO YUNI SRI RAHAYU

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Journal : Microbiology Indonesia