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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Hemera Zoa
B P Priosoeryanto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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The Ability of Immunoglobulin Yolk Recognized the Antigen in the Tissue of Ascaridia galli . Darmawi; U Balqis; M Hambal; R Tiuria; B P Priosoeryanto; E Handharyani
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1489.954 KB) | DOI: 10.5398/medpet.2012.35.3.190

Abstract

Antigen-antibody reaction is an important tool for the analysis of localization of target molecules, including antigenic protein within worm tissues. The purpose of the present research was to demonstrate the ability of immunoglobulin yolk (IgY) anti-excretory/secretory recognized the antigen in the tissue of Ascaridia galli by mean of immunohistochemistry method. The excretory/secretory protein was procured from A. galli and concentrated by mean of vivaspin 30,000 MWCO. IgY was produced by egg yolks of immunized chickens with excretory/secretory, and purified using fast protein liquid chromatography (FPLC) method. A. galli adult worms were cut in transversal and longitudinal section of the center and anterior region. Slides were incubated with both primary IgY for overnight at 4 oC and secondary antibody rabbit anti-chicken IgY HRP-conjugate for one hour at room temperature. The slides were stained with 3-amino, 9-ethylcarbazole (AEC) chromogen, counterstained with Lillie Mayer Haematoxylin, and mounted in glyserin aqueous mount.  Antigen-antibody reaction was investigated under a microscope. The result showed that antigen was appeared in the tissues such as cuticle, epicuticle, buccal cavity, and eggs inside the uterine of A. galli. This research concluded that IgY stimulated by the excretory/secretory was able to recognized the antigen scattered in the tissues of A. galli so the IgY could be applied for immunodiagnostic.
Mucosal Mast Cells Response in the Jejunum of Ascaridia galli-Infected Laying Hens . Darmawi; U Balqis; M Hambal; . Frengki; B P Priosoeryanto
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (796.113 KB) | DOI: 10.5398/medpet.2013.36.2.113

Abstract

Intestinal defense mechanism against helminthes parasitic nematode to be associated with mucosal mast cells reaction. The aim of this research was to examine the effect of infection by Ascaridia galli parasite to trigger mucosal defense based on mucosal mast cells response in laying hens. Amount of ten head laying hens 12-wk old were divided into two groups containing five chickens of each. The first group, chickens were left as un-infected controls. The second group, chickens were infected orally with 1,000 embryonated eggs of A. galli.  Mucosal mast cell responses were assayed by in situ jejunal mast cell counts in stained serial histological sections with Alcian blue (pH 0.3) and Safranin-O (pH 0.1) of the jejunum. Mucosal mast cells response were observed and counted on days 14 post infection. The result showed that A. galli infection was able to increase significantly (P<0.05) mast cells response. This research concluded that the A. galli infection can trigger the involment of mucosal mast cells response in jejunal defense of laying hens against parasitic diseases caused by A. galli.
JSPS-4 Preliminary Investigation of Bovine Respiratory Disease Complex in Indonesia S Setiyaningsih; M Shofa; R Wulansari; B P Priosoeryanto
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (560.463 KB)

Abstract

Bovine Respiratory Disease Complex (BRDC) has been recognized as a major cause of cattle morbidity and mortality throughout the world, which greatly affects beef and dairy cattle industries. It develops as a result of complex interactions between environment, cattle, and pathogens. Multiple viral or bacterial agents have been documented in BRDC cases. These include Bovine Herpesvirus 1 (BoHV-1), Bovine Viral Diarrheal Virus (BVDV), and Bovine Parainfluenza Virus-3 (BPIV-3), Bovine Respiratory Syncytial Virus (BRSV); Bovine Coronavirus, Bovine influenza D Virus, Bovine Rhinitis A and B viruses, Bovine Adenovirus, Mannheimia haemolytica (MH), Pasteurella multocida (PM), Histophilus somni (HS), Klebsiella pnemoniae, Truperella pyogenes,  Ureaplasma diversum, and Mycoplasma bovis (MB) (1). Indonesia imports considerable number of life beef and dairy cattle from Australia and New Zealand. Close proximity of cattle in a high-density during transportation and stress related to dramatic environmental changes often give rise to significant losses due to respiratory diseases. Accordingly, feedlot and dairy cattle in Indonesia are likely to have high risk of experiencing BRDC. This preliminary study was aimed to investigate the occurrence of BRDC and to identify its associated pathogens in beef and dairy cattle in limited area of western Java.
JSPS-5 Activity of Medicinal Herbs Extracts Against Bovine Respiratory Syncytial Virus Infection and Co-Infection with BRSV-Pasteurella multocida adherence on MDBK Cell N Lanniari; B P Priosoeryanto; S Setiyaningsih; M Shofa; R Wulansari; H Pisestyani
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (582.923 KB)

Abstract

Bovine respiratory disease complex (BRDC) in calves are responsible of major economic losses in both beef and dairy production (1,2). BRDC was caused by interaction of viral and or bacterial pathogens which are mutually in major cases, this combination includes bovine respiratory syncytial virus (BRSV), bovine herpes virus, bovine viral diarrhea virus, bovine parainfluenza-3 virus, Pasteurella multocida, Mannheimia haemolytica, Mycoplasma bovis, and Histophilus somni (2). BRSV is a single negative stranded RNA virus belonging to the Paramyxoviridae family as a primary etiological agent of respiratory disease in calves of beef and dairy cattle (1,2,3). BRSV is capable of initial infection or facilitates secondary infection of the lower respiratory tract by bacteria (4,5). Recently, high density of the animals movement and extreme temperatures change causing stress and going to be respiratory problems. There is currently no specific treatment for BRDC and treatment is primarily of symptomatic. Specific treatment as an antivirals are urgently required. Herbs has known as a traditional medicinal plant used as an antiviral, anti-inflammatory and immune-stimulant (6).  Given the traditional usage of herbs, this study sought to determine whether the principal bioactive ingredient of herbs possessed detectable anti-BRSV activities. However, information about antiviral treatment in Indonesia is limited. The aim of this study to determine bioactive ingredient of herbs have an anti-BRSV activities.
PF-35 Spider Silk (Nephilia sp.) as Suture Material on Blood Vessel Surgery S W Cheng; B J Widyananta; B P Priosoeryanto
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (563.567 KB)

Abstract

Surgical suture is a medical device used to hold body tissues together after an injury or surgery. Application generally involves using a needle with an attached length of thread. Surgical sutures are normally classified into two types, absorbable and non-absorbable. They can also be classified based on their construction, either mono-filament or multi-filament and also whether they are made from natural or synthetic materials. Sutures can also be classified according to their usage e.g. cardiovascular sutures, ophthalmic sutures, general sutures, orthopaedic sutures etc. Common problems associated with the choice of suture material include increased risk of infection, foreign body reactions, and inappropriate mechanical responses, particularly decreases in mechanical properties over time. Improved suture materials are therefore needed. As a high- performance material with excellent tensile strength, spider silk fibres are an extremely promising candidate for use in surgical sutures. However, the biochemical behaviour of individual silk fibres braided together has not been thoroughly investigated. In the present study, we characterise the inflammatory response produced from silk sutures and absorbance time.
Co-Authors . Darmawi . Frengki B J Widyananta E Handharyani H Pisestyani M Hambal M Shofa N Lanniari R Tiuria R Wulansari S Setiyaningsih S W Cheng U Balqis