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The Ability of Immunoglobulin Yolk Recognized the Antigen in the Tissue of Ascaridia galli . Darmawi; U Balqis; M Hambal; R Tiuria; B P Priosoeryanto; E Handharyani
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1489.954 KB) | DOI: 10.5398/medpet.2012.35.3.190

Abstract

Antigen-antibody reaction is an important tool for the analysis of localization of target molecules, including antigenic protein within worm tissues. The purpose of the present research was to demonstrate the ability of immunoglobulin yolk (IgY) anti-excretory/secretory recognized the antigen in the tissue of Ascaridia galli by mean of immunohistochemistry method. The excretory/secretory protein was procured from A. galli and concentrated by mean of vivaspin 30,000 MWCO. IgY was produced by egg yolks of immunized chickens with excretory/secretory, and purified using fast protein liquid chromatography (FPLC) method. A. galli adult worms were cut in transversal and longitudinal section of the center and anterior region. Slides were incubated with both primary IgY for overnight at 4 oC and secondary antibody rabbit anti-chicken IgY HRP-conjugate for one hour at room temperature. The slides were stained with 3-amino, 9-ethylcarbazole (AEC) chromogen, counterstained with Lillie Mayer Haematoxylin, and mounted in glyserin aqueous mount.  Antigen-antibody reaction was investigated under a microscope. The result showed that antigen was appeared in the tissues such as cuticle, epicuticle, buccal cavity, and eggs inside the uterine of A. galli. This research concluded that IgY stimulated by the excretory/secretory was able to recognized the antigen scattered in the tissues of A. galli so the IgY could be applied for immunodiagnostic.
Mucosal Mast Cells Response in the Jejunum of Ascaridia galli-Infected Laying Hens . Darmawi; U Balqis; M Hambal; . Frengki; B P Priosoeryanto
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (796.113 KB) | DOI: 10.5398/medpet.2013.36.2.113

Abstract

Intestinal defense mechanism against helminthes parasitic nematode to be associated with mucosal mast cells reaction. The aim of this research was to examine the effect of infection by Ascaridia galli parasite to trigger mucosal defense based on mucosal mast cells response in laying hens. Amount of ten head laying hens 12-wk old were divided into two groups containing five chickens of each. The first group, chickens were left as un-infected controls. The second group, chickens were infected orally with 1,000 embryonated eggs of A. galli.  Mucosal mast cell responses were assayed by in situ jejunal mast cell counts in stained serial histological sections with Alcian blue (pH 0.3) and Safranin-O (pH 0.1) of the jejunum. Mucosal mast cells response were observed and counted on days 14 post infection. The result showed that A. galli infection was able to increase significantly (P<0.05) mast cells response. This research concluded that the A. galli infection can trigger the involment of mucosal mast cells response in jejunal defense of laying hens against parasitic diseases caused by A. galli.
PENGARUH JUMLAH MIKROFILARIA PADA ANJING PENDERITA Canine Heartworm Diseases TERHADAP ANGKA KEMATIAN NYAMUK Aedes aegypti ISOLAT LAPANG (The Effect Of Microfilirae Amount On Dogs With Canine Heartworm Diseases On The Mortality Rate Of Aedes Aegypti Field Isolate) dzulfikar faizin romas; T. Fadrial Karmil; M Hanafiah; Winaruddin Winaruddin; M Hambal; Muttaqien Muttaqien; Nazaruddin Nazaruddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 5, No 4 (2021): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v5i4.9376

Abstract

Penelitian ini bertujuan mengetahui pengaruh jumlah  mikrofilaria pada anjing penderita Canine Heartworm Diseases terhadap angka kematian nyamuk Ae. aegypti. Sampel yang digunakan adalah seribu dua ratus ekor nyamuk dengan lima kali ulangan dan tiga ekor anjing dengan kategori infeksi mikrofilaremik tinggi 10.395 mf/ml, sedang 1.430 mf/ml, dan ringan 330 mf/ml serta satu anjing kontrol 0 mf/ml positif. Anjing di kandangkan dengan nyamuk, ketika nyamuk selesai menghisap kemudian dikoleksi dan diamati selama 13 hari. Data angka kematian nyamuk Ae aegypti akan dianalisis, secara statistik untuk melihat standart deviasi, dipastikan dengan uji ANOVA SPSS,   Rancangan Acak Lengkap (RAL), dan disajikan dalam bentuk grafik. Hasil penelitian menunjukan angka kematian pada perlakuan nyamuk kontrol 0 mf/ml dengan rata-rata 0.68±0.94. Sedangkan angka kematian nyamuk pada perlakuan di hari pertama pengamatan rata-rata angka kematian nyamuk pada tingkat  rendah 330 mf/ml sebanyak 12.00±0.75, sedang 22.00±0.75, dan tinggi 34.00±0.9, angka kematian mulai meningkat kembali di hari ketiga, pada tingkat rendah 330 mf/ml 9.87±0.87, sedang 13.85±1.24, tinggi 16.84±1.83. Angka kematian kembali meningkat pada hari ke sepuluh dengan rata-rata rendah 15.97±9.65, sedang 31.37±2.77, tinggi 40.22±5.95. Hasil ANOVA pada pengamatan hari ke 13, nilai p dari perlakuan adalah 0.000 (p 0.05), maka dari itu kita menolak H0. Kesimpulan semakin tinggi tingkat infeksi, semakin tinggi angka kematian nyamuk sehingga nyamuk Ae. aegypti dengan tingkat infeksi yang rendah bisa menjadi potensial vektor.(This study aimed to know the effect of the amount of microfilariae on dogs with Canine Heartworm Diseases on the mortality rate of mosquitoes Aedes Aegypti. The sample used was one thousand two hundred mosquitoes with five repeatations and three dogs with a high category of microfilaremic infection 10,395 mf / ml, moderate 1,430 mf / ml, and low 330 mf / ml and one control dog 0 mf / ml positive. The dog was caged up with mosquitoes, when the mosquitoes had finished sucking then it was collected and observed for thirteen days. The mortality rate of Ae aegypti mosquito was analyzed, statistically looked at the standard deviation, confirmed by the ANOVA SPSS Complete Random Design test, and presented in graphical form. The results showed that the mortality rate in controlled mosquito treatment 0 mf / ml was 0.68±0.94. While the mortality rate of mosquitoes in the treatment on the first day of observing the average mosquitoes mortality rate at the low level of 330 mf / ml was as much as 12.00 ± 0.75, moderate 22.00 ± 0.75, and a high of 34.00 ± 0.9, the mortality rate began increasing again on the third day, at the low level 330 mf / ml 9.87 ± 0.87, medium 13.85 ± 1.24, high 16.84 ± 1.83. The death rate again increased on the tenth day with the low average of 15.97 ± 9.65, moderate 31.37 ± 2.77, high 40.22 ± 5.95. Based on ANOVA results, the p value of the treatment was 0.000 (0.05), therefore we rejected H0. The higher the infection rate, the higher the mosquitoes mortality rate so that the mosquiotoes Ae Aegypti with the low infection rate with the fewest average number of mortality rate can be a potential vector).