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All Journal Biosaintifika: Journal of Biology & Biology Education Jurnal Kedokteran Meditek
Sri Pudyastuti
FMIPA UNNES Gd D6 Lt 1 Jln. Raya Sekaran- Gunungpati- Semarang 50229 Telp./Fax. (024) 8508033
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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EFEKTIVITAS ZPT 2,4 D PADA MEDIUM MS DAN LAMA PENCAHAYAAN UNTUK MENGINDUKSI KALUS DARI KOTILEDON KEDELAI Pudyastuti, Sri; Habibah, Noor Aini; -, Sumadi
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 1 (2012): March 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i1.2267

Abstract

Dalam upaya menghasilkan kedelai yang tahan terhadap hama penyakit, dan cekaman, maka dilakukan pemuliaan dengan kultur jaringan, melalui kalus. Penggunaan kalus sebagai tahap penelitian selanjutnya yaitu induksi variasi somaklonal atau transformasi genetik. Penelitian ini bertujuan mengetahui kondisi cahaya, konsentrasi 2,4 D optimal dalam setiap kombinasi perlakuan. Penelitian menggunakan Rancangan Acak Kelompok yang terdiri dari 2 faktor. Konsentrasi 2,4-D dan keadaan cahaya. Konsentrasi 2,4-D terdiri dari 4 taraf (0 ppm; 3 ppm; 6 ppm; dan 9 ppm;) dan 2 taraf lama pencahayaan (24 dan 0 jam). Variabel yang diamati adalah waktu muncul kalus, persentase tumbuh kalus dan berat kalus. Hasil penelitian menunjukan konsentrasi 2,4 D merupakan faktor yang mempengaruhi induksi kalus, interaksi 2,4 D dan kondisi pencahayaan tidak berpengaruh terhadap pembentukan kalus. Interaksi 2,4 D dan kondisi pencahayaan tidak ada yang efektif untuk menginduksi kalus. Berdasarkan uji Duncan dihasilkan konsentrasi 2,4-D yang paling optimal adalah 9 ppm. In an effort to produce soybean that are resistant to pests and diseases and stress, the breeding was done by tissue culture, through callus. The use of callus is the further step in the research phases of somaclonal induction or genetic transformation. This study aimed to determine the condition of light, the optimal concentration of 2,4 D in any combinations of treatment. The research was using randomized block design consisting of two factors: the concentration of 2,4 D and the light. There was 4 levels of 2,4 D concentration (0 ppm; 3 ppm; 6 ppm; 9 ppm), and 2 levels of light exposure (24 and 0 hours). The observed variable was the time of the callus emerged, the percentage of the callus growth and the weight of the callus. The result showed that the concentration of 2,4 D was the factor affecting callus induction, whereas 2,4D and the interaction of light condition was not effective to induce callus. Based on Duncan test, the optimum concentration of 2,4 D was 9 ppm.
Hubungan Penggunaan Kontrasepsi Hormonal Dengan Kejadian Preeklampsia Di RSUP Persahabatan Junnata, Agung; Marliany, Lily; Pudyastuti, Sri
Jurnal Kedokteran Meditek Vol 32 No 2 (2026): MARET
Publisher : Fakultas Kedokteran Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/jkdoktmeditek.v32i2.4120

Abstract

Introduction: Preeclampsia is one of the leading causes of maternal morbidity and mortality in Indonesia. Several studies have reported that hormonal contraception may affect blood pressure and vascular function, thereby potentially contributing to the development of preeclampsia. However, the available evidence remains inconsistent. Purpose: This study aimed to determine the association between maternal age, parity, and history of contraceptive use, particularly hormonal contraception, and the occurrence of preeclampsia among pregnant women. Methods: This study employed an observational analytic design with a cross-sectional approach using secondary data from the medical records of pregnant women at RSUP Persahabatan Jakarta during May–July 2025. A total of 138 respondents were selected using a simple random sampling technique. Univariate analysis was conducted to describe subject characteristics, while bivariate analysis was performed using the Chi-square test or Fisher’s exact test, with a significance level of (p < 0.05.) Results: The prevalence of preeclampsia was 10.87%. There was a significant association between maternal age and the occurrence of preeclampsia (p < 0.001), as well as between parity and preeclampsia (p = 0.049). A history of contraceptive use was also significantly associated with preeclampsia (p = 0.014). However, no significant association was found between the type of contraception (hormonal vs non-hormonal) or the duration of hormonal contraceptive use and preeclampsia. Conclusion: Maternal age, parity, and history of contraceptive use were associated with preeclampsia. However, the type and duration of hormonal contraceptive use were not shown to increase the risk of preeclampsia in this study population.
Co-Authors Junnata, Agung Marliany, Lily Noor Aini Habibah Sumadi -