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All Journal Biosaintifika: Journal of Biology & Biology Education Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia
Sri Pudyastuti
FMIPA UNNES Gd D6 Lt 1 Jln. Raya Sekaran- Gunungpati- Semarang 50229 Telp./Fax. (024) 8508033
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Medicine & Pharmacology Public Health

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EFEKTIVITAS ZPT 2,4 D PADA MEDIUM MS DAN LAMA PENCAHAYAAN UNTUK MENGINDUKSI KALUS DARI KOTILEDON KEDELAI Pudyastuti, Sri; Habibah, Noor Aini; -, Sumadi
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 1 (2012): March 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i1.2267

Abstract

Dalam upaya menghasilkan kedelai yang tahan terhadap hama penyakit, dan cekaman, maka dilakukan pemuliaan dengan kultur jaringan, melalui kalus. Penggunaan kalus sebagai tahap penelitian selanjutnya yaitu induksi variasi somaklonal atau transformasi genetik. Penelitian ini bertujuan mengetahui kondisi cahaya, konsentrasi 2,4 D optimal dalam setiap kombinasi perlakuan. Penelitian menggunakan Rancangan Acak Kelompok yang terdiri dari 2 faktor. Konsentrasi 2,4-D dan keadaan cahaya. Konsentrasi 2,4-D terdiri dari 4 taraf (0 ppm; 3 ppm; 6 ppm; dan 9 ppm;) dan 2 taraf lama pencahayaan (24 dan 0 jam). Variabel yang diamati adalah waktu muncul kalus, persentase tumbuh kalus dan berat kalus. Hasil penelitian menunjukan konsentrasi 2,4 D merupakan faktor yang mempengaruhi induksi kalus, interaksi 2,4 D dan kondisi pencahayaan tidak berpengaruh terhadap pembentukan kalus. Interaksi 2,4 D dan kondisi pencahayaan tidak ada yang efektif untuk menginduksi kalus. Berdasarkan uji Duncan dihasilkan konsentrasi 2,4-D yang paling optimal adalah 9 ppm. In an effort to produce soybean that are resistant to pests and diseases and stress, the breeding was done by tissue culture, through callus. The use of callus is the further step in the research phases of somaclonal induction or genetic transformation. This study aimed to determine the condition of light, the optimal concentration of 2,4 D in any combinations of treatment. The research was using randomized block design consisting of two factors: the concentration of 2,4 D and the light. There was 4 levels of 2,4 D concentration (0 ppm; 3 ppm; 6 ppm; 9 ppm), and 2 levels of light exposure (24 and 0 hours). The observed variable was the time of the callus emerged, the percentage of the callus growth and the weight of the callus. The result showed that the concentration of 2,4 D was the factor affecting callus induction, whereas 2,4D and the interaction of light condition was not effective to induce callus. Based on Duncan test, the optimum concentration of 2,4 D was 9 ppm.
Karakteristik Klinik dan Analisis Faktor Risiko yang Berperan pada Derajat Keparahan Invasi Plasenta Abnormal di RSUP Persahabatan pada Tahun 2015-2018 Feharsal, Yuri; Lisnawati, Yuyun; Pudyastuti, Sri; Khonsa, Oni; Apriliawan, Tri; Botefilia, Botefilia; Nurfauziah, Nadia; Agustin, Kindy
Majalah Kedokteran Indonesia Vol 69 No 6 (2019): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.69.6-2019-195

Abstract

Background: Morbidly adherent placenta is a condition which placenta adheres and invades deeply into the myometrium and, in some cases, until uterine serosa, thus contribute to peri-partum hemorrhage and significant maternal mortality.Purpose: To identify prevalence, clinical characteristics and their odds ratio to increasing se-verity of morbidly adherent placenta case in RSUP Persahabatan from 2015 to 2018.Methods: Cohort retrospective study.Results: From 2015 to 2018, prevalence of morbidly adherent placenta was around 0.8%. The risk factors which contribute to increasing severity of morbidly adherent placenta were: age above 35 years (OR 1.6, 95% CI 0.41-6.24) and history of Caesarean section more than 2 times (OR 1.63, 95% CI 0.41-6.46). The presence of increasing severity of morbidly adherent placenta were related to volume of blood loss more than 1000 ml during surgery (OR 2.13, 95% CI 0.52-8.76). Conclusion: The prevalence of morbidly adherent placenta at Persahabatan General Hospital in 2015-2018 was 0.8%. Age, history of Caesarean section more than 2 times, volume of blood loss, and duration of surgery were not statistically associated with adherent placenta case.
Co-Authors Agustin, Kindy Botefilia, Botefilia Khonsa, Oni Lisnawati, Yuyun Noor Aini Habibah Nurfauziah, Nadia Sumadi - TRI APRILIAWAN, TRI Yuri Feharsal