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All Journal Prosiding SNATIF ANNALES BOGORIENSES Annales Bogorienses
Azis Purwantoro
Fakultas Pertanian Universitas Gajah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Immunology & microbiology Medicine & Pharmacology

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IDENTIFIKASI SIFAT BENIH KAWISTA (Feronia limonia (L.) Swingle) UNTUK TUJUAN PENYIMPANAN Murrinie, Endang Dewi; Yudono, Prapto; Purwantoro, Azis; Sulistyaningsih, Endang
Prosiding SNATIF 2017: Prosiding Seminar Nasional Teknologi dan informatika (BUKU 1)
Publisher : Prosiding SNATIF

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Abstract

AbstrakPenanganan benih yang tepat dapat mempertahankan mutu benih selama penyimpanan atau dapat menekan laju kemunduran benih seminimal mungkin. Dalam terminologi penanganan benih terdapat tiga kelompok benih yaitu benih ortodoks, rekalsitran dan intermediate. Sampai saat ini belum diketahui apakah benih kawista masuk dalam kelompok ortodoks, rekalsitran atau intermediate sehingga dalam rangka untuk mempertahankan viabilitasnya selama dalam penyimpanan perlu dilakukan identifikasi sifat benih kawista. Tujuan penelitian adalah mengidentifikasi sifat benih kawista untuk tujuan penyimpanan. Benih kawista yang digunakan berasal dari Desa Dasun, Kecamatan Lasem, Kabupaten Rembang. Pengujian sifat benih dengan menggunakan metode yang dikemukakan Hong & Ellis (1996). Selain itu juga dilakukan pengamatan terhadap bobot kering dan kadar air benih selama perkembangan benih mulai dari antesis sampai benih masak. Hasil penelitian menunjukkan benih kawista termasuk dalam kriteria benih ortodoks, karena tetap menunjukkan viabilitas yang tinggi yang ditunjukkan oleh persentase perkecambahan yang tetap tinggi dan tidak berbeda nyata pada beberapa tingkat kadar air. Persentase perkecambahan berkisar antara 96,0-98,5% pada semua kadar air, yaitu kadar air 40,6% (kadar air setelah ekstraksi); 10,5% dan 5,5% (baik sebelum maupun setelah disimpan tiga bulan pada suhu -20 0C) dengan bobot kering kecambah yang tidak berbeda nyata.  Kata kunci: benih, kawista, ortodoks, rekalsitran
Improvement of Genetic Transformation Efficiency in Vanda tricolor Orchid Using Acetosyringone Dwiyani, Rindang; Purwantoro, Azis; Indrianto, Ari; Semiarti, Endang
ANNALES BOGORIENSES Vol 14, No 2 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/53

Abstract

Vanda tricolor  Lindl. var. suavis  is an Indonesian wild orchid which is now extremely rare in nature due to its habitat  destruction.  Development  of  an  appropriate  method  for  improving   Vanda  orchid  through  genetic modification could be valuable for horticulture and, indirectly, also for conservation. In this research, a method of  Agrobacterium-mediated transformation of two  Vanda  tricolor  obtained  from  Salak Mount, West Java and merapi Mount, Yogyakarta in Indonesia protocorms was improved using acetosyringone (AS). Concentrations of 0 and 25 ppm AS were used in transformation of pG35S binary vector containing kanamycin resistance gene into V.  tricolor  protocorms.  The  result  showed  that  25  ppm  AS  was  required  on  innoculation  with Agrobacterium  solution, without AS on cocultivation. Five week s after  treatment  on the 300  ppm kanamicyncontaining medium, green protocorms were obtained, that  was  11.01% for  V.  tricolor  from Salak Mount with pre-culture treatment prior to innoculation, 9.39% for  V.  tricolor  from Merapi  Mount with pre-culture treatment prior to  innoculation,  and  1.37%  for  V.  tricolor  from  Merapi  Mount  without  pre-culture  treatment  prior  to innoculation. The  best  condition  to  set  high  efficiency  of  transformation  is  pre -culture  protocorms  prior inoculation, soaking protocorm on 25  ppm  AS for 30 minutes, then cocultivate its on AS-free  callus induction medium Key words: Vanda tricolor, Agrobacterium, orchid protocorms, acetosyringone
Improvement of Genetic Transformation Efficiency in Vanda tricolor Orchid Using Acetosyringone Dwiyani, Rindang; Purwantoro, Azis; Indrianto, Ari; Semiarti, Endang
Annales Bogorienses Vol. 14 No. 2 (2010): Annales Bogorienses
Publisher : BRIN

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Abstract

Vanda tricolor Lindl. var. suavis is an Indonesian wild orchid which is now extremely rare in nature due to its habitat destruction. Development of an appropriate method for improving Vanda orchid through genetic modification could be valuable for horticulture and, indirectly, also for conservation. In this research, a method of Agrobacterium-mediated transformation of two V. tricolor obtained from Salak Mount, West Java and Merapi Mount, Yogyakarta in Indonesia protocorms was improved using acetosyringone (AS). Concentrations of 0 and 25 ppm AS were used in transformation of pG35S binary vector containing kanamycin resistance geneinto V. tricolor protocorms. The result showed that 25 ppm AS was required on inoculation with Agrobacterium solution, without AS on cocultivation. Five weeks after treatment on the 300 ppm kanamicyn containing medium, green protocorms were obtained, that was 11.01% for V. tricolor from Salak Mount with pre-culture treatment prior to inoculation, 9.39% for V. tricolor from Merapi Mount with pre-culture treatment prior to inoculation, and 1.37% for V. tricolor from Merapi Mount without pre-culture treatment prior to inoculation. The best condition to set high efficiency of transformation is pre-culture protocorms prior inoculation, soaking protocorm on 25 ppm AS for 30 minutes, then co-cultivate its on AS-free callus induction medium.
Co-Authors Ari Indrianto Endang Semiarti Endang Sulistyaningsih Issirep Sumardi Prapto Yudono Rindang Dwiyani Sulistiono Syamsul Arifin