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All Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Asvin Nurulita
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience

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PNEUMATIC TUBE TERHADAP DARAH RUTIN DAN LAKTAT DEHIDROGENASE Liong Boy Kurniawan; Asvin Nurulita; Uleng Bahrun
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 2 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i2.1087

Abstract

The transportation of laboratorial samples with pneumatic tube system spends less time than when were handled by courier.Pneumatic tube system produces minor vibrations on sample due to the velocity changes during transportation and may cause changeson the blood cells and haemolysis. The aim of this study is to know the effect of sample transportation with pneumatic tube on bloodcells and its effect on hemolysis. A cross sectional study was performed at Dr. Wahidin Sudirohusodo Hospital, Makassar in July 2013.Routine blood, electrolyte and LDH were tested in 12 out-patients. The researchers collected two (2) samples, for each EDTA tube (routineblood tests) and serum (electrolyte and LDH). The samples were transported using pneumatic tube and the paired samples were sentby courier. The result then were analyzed with Paired T-Test. There were no significant difference of routine blood test results betweensamples sent by pneumatic tube and courier except RDW. RDW were higher in samples which were sent by pneumatic tube comparedto those brought by the courier (18.72±2.70% vs 17.83±2.36%, p=0.007). The electrolyte levels sent by both methods there were nosignificant difference, but the LDH levels were higher in samples sent by pneumatic tube (472.08±100.44 U/L vs 331.25±94.19 U/L,p=0.000). Based on this study, in common can be concluded that the pneumatic tube system does not effect on the routine blood testresults, except on RDW and does not cause changes due to haemolysis (on electrolyte) except the LDH levels elevates. So based on thisstudy, it is recommended to send samples for LDH test only by courier.
ANALYSIS OF LACTIC AND HEMATOCRIT LEVELS OF BLOOD STORAGE IN DR. WAHIDIN SUDIROHUSODO GENERAL HOSPITAL BLOOD BANK Rysna Wahyu; Asvin Nurulita; Rachmawati Muhidin
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 3 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i3.1450

Abstract

The components of Packed Red Cells (PRC) are transfused to patients in order to repair oxygen transportation to tissues. The blood is stored at 2-6oC to delay red blood cells metabolism during storage. Red blood cells undergo structural and functional changes biochemically which affect their viability and function. This is a prospective cohort study with time series design. Samples were taken from fresh blood PRC which were moved to transfer bag for approximately 20 mL, then stored in the refrigerator. Lactic acid and hematocrit levels were assessed with spectrophotometry and flow cytometry methods on day 1, day 4, and day 8 of storage in the Dr. Wahidin Sudirohusodo General Hospital Blood Bank. Statistical tests used were Friedman and Wilcoxon. Statistical results are significant if p < 0.05. Total samples were 15 fresh blood PRC. Friedman statistical test showed a significant difference in lactic level (p < 0.001) and hematocrit level (p=0.012) on day 1, day 4, and day 8 of storage. Wilcoxon test showed significantly higher lactic level between day 4 and day 1 (p < 0.01); day 8 and day 1 (p < 0.01); day 4 and day 1 of storage (p < 0.01). Hematocrit level between day 4 and day 1 (p < 0.05); day 8 and day 1 (p < 0.05) were significantly higher; day 8 and day 4 of storage (p > 0.05) showed insignificant difference. Results showed that lactic and hematocrit levels of PRC stored blood were increased according to storage duration. Packed red cells blood is recommended to be given in < 6 days for lower acidosis risk. Further studies are also recommended with a shorter interval of assessment and a bigger sample size.
KADAR KALIUM DI PACKED RED CELLS SIMPANAN Angeline Sutjianto; Asvin Nurulita; Fitriani Mangarengi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1083

Abstract

Blood transfusion, is not only transferring the blood cells to the recipient, but also transferring other components such as: glucose,lactate, and potassium. When the blood is stored, many alterations occur in its cmponents, particularly a decrease of adenosine5-triphosphate (ATP) and pH, hemolysis, and an increase of potassium levels as well. One of the transfusion complications that should beavoided is hyperkalemia due to the accumulation of potassium that leaks during the storage. However, hyperkalemia related to transfusiondepends not only on the potassium level in the blood unit, but also on its volume and the rate of its blood administration as well. Theaim of this study was to know the potassium levels in stored Packed Red Cell (PRC). A cohort study was done from May–July 2010. Theresearchers used 48 samples from 16 PRCs derived from 16 donors. The samples were drawn from the of PRC hose’s plasma that had beensealed. The potassium levels were measured on the first day, 10th day, and 20th day of the blood collection. The mean potassium levelon the first day was 3.79 mmol/L, 10th day was 12.22 mmol/L and 20th day was 19.77 mmol/L. Comparison of the potassium levelsbetween the first and 10th day, between first and 20th day, and between 10th and 20th day showed a significant difference (p=0.00). Theincrease of potassium levels in the PRC coincide with the storagetime.
UJI GLUKOSA DARAH ANTARA METODE HEKSOKINASE DENGAN GLUKOSA OKSIDASE DAN GLUKOSA DEHIDROGENASE DI DIABETES MELITUS Baharuddin Baharuddin; Asvin Nurulita; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 2 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i2.1102

Abstract

Diabetes mellitus is a group of metabolic diseases characterized by chronic hyperglycemia that occurs because of abnormalities insulinsecretion, insulin or both. Diabetes mellitus diagnosis is established on the basis of examination of blood glucose levels. The recommendedblood glucose test is enzymatic method by hexokinase using venous blood plasma, while for monitoring the outcomes of treatment: bloodglucose test can be done by rapid test using capillary blood. The purpose of this study was to know the test result of blood glucose betweenhexokinase method (ABX Pentra 400), glucose oxidase (StatStrip Xpress, Super Glucocard II) and glucose dehydrogenase methods(Accu-Chek Performa) by comparing them Cross sectional study was conducted on 50 samples of patient hospitalized at Dr. WahidinSudirohusodo Hospital Makassar. The blood glucose test were performed at the Departement of Clinical Pathology from June up to July2012. Each plasma sample was tested using ABX Pentra 400, StatStrip Xpress, Super Glucocard II and Accu-Chek Performa. The datawere analyzed using Kolmogorov Smirnov and Mann Whitney test. Based from this study was showed that there was no difference in bloodthe level using a related (glucose) oxidase (StatStrip Xpress, Super Glucocard II) and its dehydrogenase method (Accu-Chek Performa)compared with hexokinase method (ABX Pentra 400) with p value > 0.05. The result obtained in this study showed that StatStrip Xpress,Super Glucocard II and Accu-Chek Performa can be used as a rapid test for monitoring blood glucose in hospitalized patients.
NEUTROPHIL/LYMPHOCYTE COUNT RATIO ON DENGUE HEMORRHAGIC FEVER Irmayanti Irmayanti; Asvin Nurulita; Nurhayana Sennang
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 3 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i3.1200

Abstract

Infeksi virus dengue adalah salah satu masalah kesehatan masyarakat yang menimbulkan dampak sosial dan ekonomi. Pemeriksaanrasio netrofil/limfosit sangat mudah dan cepat dilakukan. Rasio netrofil/limfosit dihubungkan dengan perembesan plasma di pasienDBD. Untuk mengetahui rasio netrofil/limfosit pasien DBD berdasarkan derajat DBD. Penelitian ini merupakan penelitian retrospektifdengan mengambil data rekam medis RSUP Dr. Wahidin Sudirohusodo Makassar masa waktu Juni 2013–Juni 2015. Uji statistikdilakukan dengan uji Mann-Whitney, uji t dan uji kenasaban Spearman. Didapatkan 96 subjek penelitian yang memenuhi patokankesertaan. Rerata umur subjek penelitian 53,5 tahun (18–89), sebagian besar laki-laki 56,25% dan perempuan 43,75%. Uji Mann-Whitney menunjukkan median leukosit grade I 4,45 (1,10–28,80), grade II 3,25 (1,60–9,20) dengan p=0,03. Median netrofil gradeI 2,41 (0,47–24,65), grade II 1,16 (0,29–6,50) dengan p=<0,01. Median trombosit grade I 113,50 (5,00–342,000), grade II 76,50(3,00–274,00) dengan p=0,009. Rasio netrofil/limfosit grade I 2,19 (0,61–17,25), grade II 0,80 (0,18–5,91) dengan p=<0,01. Ujikenasaban Spearman didapatkan nilai p<0,001 menunjukkan kenasaban antara rasio netrofil/limfosit dan derajat DBD bermakna.Nilai kenasaban Spearman sebesar -0,68 menunjukkan hubungan terbalik, semakin rendah rasio netrofil/limfosit, semakin berat derajatDBD. Uji t menunjukkan tidak ada perbedaan bermakna nilai limfosit dan hematokrit pasien DBD grade I dan II. Rasio netrofil/limfositpada DBD grade I lebih tinggi daripada grade II, semakin rendah rasio netrofil/limfosit semakin berat derajat DBD.
CORRELATION OF ANTINUCLEAR ANTIBODY PROFILE WITH HEMATOLOGIC AND RENAL DISORDERS IN SYSTEMIC LUPUS ERYTHEMATOSUS Chelvi Wijaya; Asvin Nurulita; Uleng Bahrun
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 2 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i2.1137

Abstract

Systemic Lupus Erythematosus (SLE) adalah penyakit autoimun dan bersifat multi organ. Kelainan hematologi sering ditemukandi penyakit ini, begitu juga dengan kelainan ginjal yang merupakan salah satu faktor yang sangat berpengaruh. Uji ANA profile dapatmengetahui subtipe antibodi antinuklear yang khas. Autoantibodi tersebut diduga berhubungan dengan manifestasi klinis. Penelitian inimerupakan penelitian analitik retrospektif di Laboratorium Patologi Klinik dan Instalasi Rekam Medik RSUP. Dr. Wahidin SudirohusodoMakassar dengan mengambil data hasil ANA profile, darah rutin dan urinalisis pasien terduga SLE masa waktu Januari 2014–Juli 2016.Data dikelompokkan menjadi SLE dan nonSLE. Analisis statitik dengan uji Chi Kuadrat dan Fisher. Dari 72 sampel, 39 dengan diagnosaakhir SLE. Terdapat hubungan bermakna antara anti RNP/Sm, Sm, SS-A, Ro-52, dsDNA, Nucleosome, Histone, Ribosomal P denganSLE (p<0,05). Terdapat hubungan bermakna antara anti dsDNA (p=0,029) dan anti nucleosome (p=0,037) dengan anemia serta antidsDNA (p=0,013) dan anti nucleosome (p=0,036) dengan gangguan ginjal. Tidak ditemukan hubungan bermakna antara autoantibodidalam penelitian ini dengan leukopenia, limfopenia dan trombositopenia. Anti RNP/Sm, Sm, SS-A, Ro-52, dsDNA, nucleosome, Histones,Ribosomal P berhubungan dengan SLE. Anti dsDNA dan anti nucleosome berhubungan dengan anemia dan gangguan ginjal padaSLE, sehingga mungkin dapat digunakan untuk meramalkan kejadian tersebut, walaupun dibutuhkan penelitian lanjutan untukmembuktikannya. Tidak ditemukan autoantibodi yang dapat dihubungkan dengan leukopenia, limfopenia dan trombositopenia.
PENILAIAN UJI TROPONIN I DENGAN POINT OF CARE TESTING Sheila Febriana; Asvin Nurulita; Uleng Bahrun
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i2.1112

Abstract

Troponin I is a cardiac biomarker recomended by The Third Global Myocardial Infarction Task Force World Health Organisation(WHO). Troponin plays a central role as a relevant biomarker that require reliable samples, methods, device and efficiency of time.Selecting the device, methods and sample used in the assay may affect the results and turn arround time. The aim of this study is toknow troponin I result using Point of care Testing device with a flourescence immunoassay methods using whole blood and laboratorybasedanalysis device with Enzyme-Linked Fluorescent Assay (ELFA) methods using serum by evaluation. Cross sectional study was heldon 50 subjects in Wahidin Sudirohusodo hospital during the period between July-August 2015, those who suspected suffering acutecoronary syndome (ACS) and underwent troponin I test ordered by the physician and also had whole blood sample. The subjects arearound 51.96±12.80 year old and most of them are men (62%). The mean consentration of troponin I with laboratoric-based analysis is0.50±1.69 μg/L and with POCT is 0.51±1.77. The Pearson correlation test shows the correlation (r) is 0.99 with the p value is <0.001.Bland and Altman methods show the mean difference between two assays is 0.014μg/L (95% confidence interval, -0.015; 0.043) withthe limit of agreement -0.19 to 0.22. Based on this study, it can be concluded that troponin I assay using POCT device can be used tosupport ACS diagnosis precisely and rapidly. It is suggested to perform futher study with concern on the patient’s clinical condition aswell as the diagnosis, so it can evaluate the device performance to measure troponin I levels consistently with the clinical condition.
CARCInoeMBRYonIC AnTIGen (CEA) DI KANKER KOLOREKTAL Nur Rahmi Raehaan; Asvin Nurulita; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.465

Abstract

Colorectal cancer is a common gastrointestinal malignancy of the colon and rectum. According to the American Society of Clinical Oncology (ASCO) in 2006, preoperative CEA level is useful in dtermining the tumour stage, plan of action and monitoring therapeutic response during the active treatment. Several factors which influence CEA level in patients with colorectal cancer is the staging and the degree of tumour, liver function, and as well as its location. This retrospective study is aimed to know the preoperative CEA levels in 51 patients with colorectal cancer and to compare the levels of CEA based on tumour stage, degree of tumour based on histopathology and tumour location.. This study was carried out at the Dr.Wahidin Sudirohusodo Hospital (RSWS), Makassar during January 2009−December of 2011. The researchers found a significant difference between the levels of CEA with the tumour stage (p=0.000) and its relation with the degree of the tumour (p=0.002), however, based on the tumour location (p=0.585) there was no significant difference between the levels of CEA. In conclusion, it was found that the higher the tumour stage, the higher the levels of the produced CEA. A well differentiated tumour of colorectal cancer produced a higher level of CEA compared to the moderate or poor-differentiated tumours.
ANALYSIS OF LACTIC AND HEMATOCRIT LEVELS OF BLOOD STORAGE IN DR. WAHIDIN SUDIROHUSODO GENERAL HOSPITAL BLOOD BANK Rysna Wahyu; Asvin Nurulita; Rachmawati Muhidin
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 25 No. 3 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i3.1450

Abstract

The components of Packed Red Cells (PRC) are transfused to patients in order to repair oxygen transportation to tissues. The blood is stored at 2-6oC to delay red blood cells metabolism during storage. Red blood cells undergo structural and functional changes biochemically which affect their viability and function. This is a prospective cohort study with time series design. Samples were taken from fresh blood PRC which were moved to transfer bag for approximately 20 mL, then stored in the refrigerator. Lactic acid and hematocrit levels were assessed with spectrophotometry and flow cytometry methods on day 1, day 4, and day 8 of storage in the Dr. Wahidin Sudirohusodo General Hospital Blood Bank. Statistical tests used were Friedman and Wilcoxon. Statistical results are significant if p < 0.05. Total samples were 15 fresh blood PRC. Friedman statistical test showed a significant difference in lactic level (p < 0.001) and hematocrit level (p=0.012) on day 1, day 4, and day 8 of storage. Wilcoxon test showed significantly higher lactic level between day 4 and day 1 (p < 0.01); day 8 and day 1 (p < 0.01); day 4 and day 1 of storage (p < 0.01). Hematocrit level between day 4 and day 1 (p < 0.05); day 8 and day 1 (p < 0.05) were significantly higher; day 8 and day 4 of storage (p > 0.05) showed insignificant difference. Results showed that lactic and hematocrit levels of PRC stored blood were increased according to storage duration. Packed red cells blood is recommended to be given in < 6 days for lower acidosis risk. Further studies are also recommended with a shorter interval of assessment and a bigger sample size.
Co-Authors Angeline Sutjianto Baharuddin Baharuddin Chelvi Wijaya Fitriani Mangarengi Irmayanti Irmayanti Liong Boy Kurniawan, Liong Boy Mansyur Arif Nur Rahmi Raehaan Nurhayana Sennang Rachmawati Muhidin Rysna Wahyu Sheila Febriana Uleng Bahrun