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IDENTIFIKASI BAKTERI AEROB GRAM NEGATIF DAN GRAM POSITIF MENGGUNAKAN METODE KONVENSIONAL DAN OTOMATIK Patricia M. Tauran; Irda Handayani; Nurhayana Sennang
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 2 (2013)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i2.1065

Choosing the method of bacteria identification is crucial to obtain accurate and quick results. This study will analyze the identificationresults of Gram negative and Gram positive from aerobic bacteria by examination using conventional and automatic methods at Dr.Wahidin Sudirohusodo Hospital Laboratory. A total of 85 samples consisting of 66 Gram negative bacteria and 19 Gram positive bacteriawere identified using conventional and automated methods. In this study, there was some correspondent identification result betweenthe conventional as well as the automated methods, namely 31.5% for Gram negative bacteria and 30.8% for Gram positive bacteria.However, the non-correspondent identification result between conventional and automated methods was found greater, namely, 68.5%for Gram negative bacteria and 69.2% for Gram positive bacteria. The non-correspondent identification result was due to the developmentof bacterial taxonomy and the differences of numbers and types of the biochemical tests between conventional and automatic methods.Bacteria identification using automated method is more accurate and faster than the conventional method, so it is recommended usingthis particularly for the laboratory and educational referral center.

POLA KETAHANAN (RESISTEN) DAN KEPEKAAN (SENSITIVITAS) KUMAN TERHADAP ANTIMIKROBA Y F Tallulembang; Nurhayana Sennang; Benny Rusli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.958

In the developing countries including Indonesia, there still have problems with infectious diseases. Generally the hospitalized patientsin the Surgery section through have antimicrobial therapy. The treatment is intended to prevent nosocomial infection that usually foundin overnight treatment patients at the department of surgery. This study is carried out to know the pattern of micro-organism’s resistanceand sensitivity against various antimicrobial at department of surgery, Dr. Wahidin Sudirohusodo Hospital Makassar, January–June2008. A descriptive study of retrospectively collective data was carried out on 160 specimens by sensitivity test at the department ofsurgery, Dr. Wahidin Sudirohusodo Makassar Hospital, started from January up to June 2008. The five common bacterial found in thisstudy where Staphylococcus epidermidis, Klebsiella pneumonia, Enterobacter agglomerans, Staphylococcus saprophytic and Escherichiacoli. The sensitive antimicrobial drugs found where Vancomicin, Sulbactam/Cepoperazone, Cefepim, Gentamicin, Ceftazidim, Novobiocin,followed by the resistance antimicrobial were Methicillin, Tetracycline, Cefuroxim, Erythromycin, and Doxyciclin. The most bacterial typefound was Klebsiella pneumoniae, the most sensitive antimicrobial was Vancomicin and the most resistances were Methicillin.

JAMUR DI PERALATAN neonATAL InTenSIve CARe unIT Ariani Said; Irda Handayani; Nurhayana Sennang
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.468

Fungus is the one of the micro organisms that causes nosocomial infections. Fungal infections are becoming more frequent occurred from the irrational use of broad spectrum antibiotics, steroids, cytostatica drugs, and chronic disease, and malignancy, babies with low birth weight and patients with decreased immunity. Neonatal Intensive Care Unit (NICU) is a room with specialized treatment, care and equipment to treat infants with high risk. The purpose of this study was to know by identification of the fungus species on the instrument at the NICU of dr. Wahidin Sudirohusodo hospital, Makassar at June 2011. The study design was a cross sectional one. The samples are the incubators and the Continuous Positive Airway Pressure (CPAP) at the NICU of dr. Wahidin Sudirohusodo hospital. The sampling was done by rubbing swab on the incubator and CPAP. The identification of the fungus were carried out with lacto phenol cotton blue staining performed at the Central Public Health Laboratory, Makassar. The Streptomyces sp and Penicillium sp were identified at four incubators, Candida sp was identified at one incubator and so was the Aspergillus sp identified; The fungus that identified at the CPAP was only Penicillium sp. Based on this study it can be concluded that Penicillium sp, Streptomyces sp, Aspergillus sp, as well as Candida sp were found at all incubators and only Penicillium sp was found at CPAP.

NEUTROPHIL/LYMPHOCYTE COUNT RATIO ON DENGUE HEMORRHAGIC FEVER Irmayanti Irmayanti; Asvin Nurulita; Nurhayana Sennang
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 3 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i3.1200

Infeksi virus dengue adalah salah satu masalah kesehatan masyarakat yang menimbulkan dampak sosial dan ekonomi. Pemeriksaanrasio netrofil/limfosit sangat mudah dan cepat dilakukan. Rasio netrofil/limfosit dihubungkan dengan perembesan plasma di pasienDBD. Untuk mengetahui rasio netrofil/limfosit pasien DBD berdasarkan derajat DBD. Penelitian ini merupakan penelitian retrospektifdengan mengambil data rekam medis RSUP Dr. Wahidin Sudirohusodo Makassar masa waktu Juni 2013–Juni 2015. Uji statistikdilakukan dengan uji Mann-Whitney, uji t dan uji kenasaban Spearman. Didapatkan 96 subjek penelitian yang memenuhi patokankesertaan. Rerata umur subjek penelitian 53,5 tahun (18–89), sebagian besar laki-laki 56,25% dan perempuan 43,75%. Uji Mann-Whitney menunjukkan median leukosit grade I 4,45 (1,10–28,80), grade II 3,25 (1,60–9,20) dengan p=0,03. Median netrofil gradeI 2,41 (0,47–24,65), grade II 1,16 (0,29–6,50) dengan p=<0,01. Median trombosit grade I 113,50 (5,00–342,000), grade II 76,50(3,00–274,00) dengan p=0,009. Rasio netrofil/limfosit grade I 2,19 (0,61–17,25), grade II 0,80 (0,18–5,91) dengan p=<0,01. Ujikenasaban Spearman didapatkan nilai p<0,001 menunjukkan kenasaban antara rasio netrofil/limfosit dan derajat DBD bermakna.Nilai kenasaban Spearman sebesar -0,68 menunjukkan hubungan terbalik, semakin rendah rasio netrofil/limfosit, semakin berat derajatDBD. Uji t menunjukkan tidak ada perbedaan bermakna nilai limfosit dan hematokrit pasien DBD grade I dan II. Rasio netrofil/limfositpada DBD grade I lebih tinggi daripada grade II, semakin rendah rasio netrofil/limfosit semakin berat derajat DBD.

RESISTENSI TERHADAP METHICILLIN (METHICILLIN RESISTANT) STAPHYLOCOCCUS AUREUS DI INSTALASI RAWAT INAP Wildana .; Nurhayana Sennang; Benny Rusli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1047

Methicillin Resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen worldwide. MRSA infection typically aggravatesthe patient condition. MRSA infection increases morbidity and mortality. The study was aimed to find out the MRSA occurrence inDr. Wahidin Sudirohusodo Hospital Makassar patients during July 2008–June 2009. A retrospective study was performed using datafrom the medical records including the results of culture and antimicrobial susceptibility test in Dr. Wahidin Sudirohusodo HospitalMakassar. Among 1082 results of the culture test, 5.2% were identified as Staphylococcus aureus, consist of 51.8% MSSA (MethicillinSensitive Staphylococcus aureus) and 48.2% MRSA. Most of the MRSA patients were treated in orthopaedic surgery (30%), internal(22%), and paediatric (19%) wards. Based on the clinical conditions, most of the patients were in post surgery care (44.4%), pneumonia(18.5%), and diabetic foot (7.5%). All of the MRSA isolates were multiresistant (resistant to three or more antimicrobials) but 96%remain sensitive to vancomycin. It was concluded that most of MRSA patients were staying in the orthopaedic surgery ward. Based onthis clinical condition, most of the patients were in the post surgery care. All of the MRSA isolates were multiresistant, but most of themremain sensitive to vancomycin.

RERATA VOLUME TROMBOSIT, HITUNG LEUKOSIT DAN TROMBOSIT DI APENDISITIS AKUT Jayadi Festiawan; Nurhayana Sennang; Ibrahim Abdul Samad
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1075

The diagnosis of acute appendicitis and its complications still depends on the radiological examination and laparoscopy techniques.The aim of this study was to know the Mean Platelet Volume (MPV), WBC and platelet value in the diagnosis of acute appendicitis,associated with the incidence of existing complications. This research was carried out by a retrospective study by taking secondary datafrom the medical records, including the results of routine blood tests of acute appendicitis patients with and without complications atthe Dr. Wahidin Sudirohusodo Hospital between May 2011− May 2012. The data obtained in the study were MPV, White Blood Cell(WBC) and platelet count which were then analyzed using independent T test. The results obtained from 44 samples were that MPVvalues showed a significant difference (p=0.02), while WBC (p=0.12) and platelet count (p=0.13) were statistically not significantlydifferent between patients with and without complications. In conclusion, it was found that acute appendicitis with complications hadhigher MPV average values than those without complications and although there were significant differences between them, these werestill within the normal values.

KEPEKAAN ANTIMIKROBA KULTUR DARAH DI SEPSIS NEONATAL Tajuddin Noor; Nurhayana Sennang; Benny Rusli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 1 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i1.388

Sepsis was one of the morbidity and mortality causes in neonatal. The diagnosis and treatment requires the bacterial identification and selection of sensitive antimicrobials. The aim of this study was to know the bacterial pattern and antimicrobial sensitivity of blood culture in the suspected neonatal sepsis patients who were treated at NICU in Dr.Wahidin Sudirohusodo Hospital Makassar. A retrospective study was conducted with secondary data from the culture and antimicrobial susceptibility test between the period of June 2010−July 2011. In this present study was found that from the total 91 blood culture isolates, bacteria Gram-negative group was 85.7% and Grampositive was 14.3% and the isolate encountered in order of frequency were Alkali genes faecalis 50.5%, Klebsiella pneumonia 25.3%, and Staphylococcus epidermidis 9.9%. In the Gram-negatives group, the isolate often encountered were Alkali genes faecalis 59.0%, Klebsiella pneumonia 29.4% and Enterobacter spp 6.4% while in the Gram-positive group were found Staphylococcus epidermidis 69.2% and Staphylococcus saprophytic 23.1%. The more sensitive antimicrobal that belong to Gram-negative group were Meropenem 94.4%, Levofloxacin 92.1%, and Ceftazidime 77.0% while the more resistant were Ampicillin 94.6%, Gentamycin 89.1% and Cefuroxime 82.7%. The more sensitive antimicrobal that belong to Gram-positive group were Vancomycin and Chloramphenicol 91.7% and Novobiosin 76.9% while the more resistant were Gentamycin and Ceftriaxone 100.0% and Amoxicillin 91.7%. Based on this study it can be concluded that Gram-negative aerobe bacteria was more common than the Gram-positive one. Meropenem, Levofloxacin and Ceftazidime antimicrobal were high sensitive to Gram-negative while Vancomycin, Chloramphenicol, and Novobiocin were high sensitive to Gram-positive. The resistance of Ampicillin and Gentamycin were found in both bacterially groups of sepsis suspected neonatal patients in NICU

KUMAN DAN KEPEKAAN ANTIMIKROBA DI KASUS PATAH TULANG TERBUKA Yanty Tandirogang; Tenri Esa; Nurhayana Sennang
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 2 (2013)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i2.1062

About 70% of all nosocomial infections occur in surgical patients. In open fracture, there is contact with the environment or unsterilebody compartment, so that bacterial contamination may occur and cause infection. Besides debridement, prophylactic antibiotics havebeen used as a standard procedure in the open fracture management. This procedure may cause antibiotics resistance leading to increasethe number of infections. The aim of this retrospective study was to know the characteristics, microbial pattern, and sensitivity of 35cultures and sensitivity test of open fracture patients in Orthopaedics Department of Wahidin Sudirohusodo Hospital during the period ofJune 2009–June 2010. The most common bacteria found were Proteus mirabilis (26%), Klebsiella pneumonia (14. 8%), and Providenciaalcalifaciens (14%). The sensitivity test for antimicrobials showed that most of the 19 antimicrobials, were resistant. The sensitiveantimicrobial is Meropenem.

ANALISIS CD4 PADA PENATALAKSANAAN PASIEN KOINFEKSI HIV-TB Nursin Abd. Kadir; Nurhayana Sennang; Hardjoeno .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.991

The Human Immunodeficiency Virus (HIV) epidemic has increased the burden of tuberculosis (TB) among young adults, especially in populations where the prevalence of TB infection is high like Indonesia. TB is the most common opportunistic infection on HIV patients(50%) in developing countries. CD4 also known as T helper lymphocytes are coordinators of body`s immune response, and it has beendeclining in HIV infection and be worsened by TB infection. CD4 count are standard laboratory marker of disease progression to followupandprognosisantiretroviraltherapyinHIVinfection.AimstoseethevalueofCD4countofHIV-TBco-infectedpatientsbeforeandafterantituberculosistogetherwithantiretroviraltherapy.AlongitudinalstudywasconductedbycollectingsecondarydatafromthemedicalrecordandtheresultsofClinicalPathologyLaboratoryofHIV-TBco-infectedpatientsatWahidinSudirohusodoHospitalperiodJuly2007–August2008.DatawereanalyzedbyWilcoxonSignedRankTestandMannWhitneyTestwitha=0.05.Totalsamples(n)were20patients(14patientswithcontinoustherapyand6uncontinoustherapy).WefounddifferentmeansbetweenCD4countbeforeandafterTherapy.CD4countbeforetherapywas71.15±81.04andaftertherapywas114.95±109.71(p=0.089)withWilcoxonSignedRankTest.Theanalyzedwerecontinuedbydividedsamplesincontinousgroupcomparedwithuncontinousgrouptherapy.TheresultshowedtheCD4alterationincontinousgroupwas853.8%anduncontinousgroupwas-56.6%wherep=0.000(MannWhitneytest).CD4counthasincreasedsignificantlyinHIV-TBco-infectedpatients8.5foldfromCD4baselinewithcontinoustherapyandtheuncontinous one has decreased 0.5 fold from CD4 baseline.

MALARIA KONGENITAL Sri Wahyunie S; Nurhayana Sennang; D. Daud; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 2 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i2.1109

Congenital Malaria is an infectious disease caused by the malaria parasite that is transmitted from mother to child through theplacenta during pregnancy or at delivery. Clinical manifestations which may arise due to Plasmodium infection are: the irritability,fever, anaemia, jaundice and hepatosplenomegaly. The incidence of congenital malaria according to the National Basic Health Research2010 is only about 0.3%. Forty two days old male baby with the main complaints fever and pale since he was three (3) weeks old. Fromthe physical examination the reviewer found anaemia, jaundice and splenomegaly. Plasmodium vivax was detected by serologic andmicroscopic examination. From the pregnancy history of mother the reviewer found that at the age of seven (7) months of pregnancyshe suffered from malaria caused by Plasmodium vivax the same as the type of Plasmodium infected the baby. The baby was born innon malaria endemic area which enhanced the diagnosis of congenital malaria of this patient. The patient was fully recovered aftertreated with dehydroartemisin piperaquin and the reviewer reported one case of congenital malaria, forty twodays old male baby. Thediagnosis was made based on the malaria history of mother at seven (7) month of pregnancy, the serologic and microscopic examinationfrom the patient blood and the baby was born in a non malaria endemic area. The prognosis of patient with congenital malaria causedby Plasmodium vivax generally was good. The clinical condition was improved and fully recovered after treated with dehydro-artemisinpiperaquin.

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