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All Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Hardjoeno .
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience

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CREATINE KINASE MYOCARDIAL BAND, MYELOPEROXIDASE, MATRIX METALLOPROTEINASE-9 DAN TROPONIN T PADA IMA IA Hutagalung; I Patellongi; R Pakasi; M Arif; AA Mappahya; Hardjoeno .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 3 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i3.1055

Abstract

In many patients presenting with chest pain symptoms at the emergency room, cardiac markers are measured not only to detect acutemyocardial infarction (AMI) but to exclude them too. Presently, the only biochemical markers used for diagnosis of MI are creatine kinasemyocardial band (CKMB) and troponin T (cTnT) as the markers of myocardial necrosis. Other biochemical markers have been sought tofind marker which can reflect the important, upstream processes in the pathophysiology of MI that therefore might give an earlier signalof ongoing MI. These markers are myeloperoxidase (MPO) and matrix metalloproteinase-9 (MMP-9) as markers of plaque destabilization.The aim of this study was to analyze the CKMB, MPO, MMP-9 activityies and troponin T level in MI. A cross sectional study had beendone at the Cardiovascular Centre Unit and Internal Ward of Dr. Wahidin Sudirohusodo Hospital during April up to August 2010 period.The laboratory tests were done at the Clinical Pathology Laboratory of Dr. Wahidin Sudirohusodo Hospital, Makassar and the Researchand Esoteric Unit of Prodia Clinical Laboratory, Jakarta. Ffifty-three patients with AMI had been examined. The results showed that themeans of CKMB, cTnT, MPO and MMP-9 were 52.4 U/L, 2.0 ng/mL, 217.9 ng/mL and 920.3 ng/mL, respectively. MMP-9 had positivelyvalue (69.8%) higher than cTnT (60.4%), MPO (58.5%) and CKMB (45.3%). Troponin T and MMP-9 were higher in patients with highCKMB. The higher level of CKMB will be the higher MPO, MMP-9 and cTnT. There were strong correlation between MPO and MMP-9, alsobetween CKMB and Troponin T. Troponin T and CKMB were lower in inferior wall infarction compared with anterolateral/anteroseptal wallinfarction. The most sensitive biochemical marker in patients with AMI is the MMP-9. The MMP-9 usage is suggested to assist the diagnosisof AMI. It is suggested a further study to establish the specificity of MMP-9 in all patients suffering with chest pain.
HUBUNGAN ANTARA KADAR HEMOGLOBIN DENGAN KADAR KREATININ SERUM PENDERITA PENYAKIT GINJAL MENAHUN (KRONIS) Rosnety, .; M Arif; Hardjoeno .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 13, No 3 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v13i3.908

Abstract

Chronically Kidney Disease (CKD) is the health problem of society worldwide, included Indonesia which its amount will increase.Clinical manifestation often following CKD is anaemia which will be severe with the increasing of serum creatinine values. To determinefrequent distribution of anaemia degree and assessing relationship between haemoglobin (Hb) and creatinine concentration in CKDpatients. Retrospective Descriptive Study in CKD patients with anaemia. In the period of May-September 2006, from 50 CKD patientsat the Department of Internal Medicine, Dr.Wahidin Sudirohusodo Hospital Makassar, 38 patients (76%) suffered severe anaemia(Hb < 8 g/dl). Mean value of Hb was 6,85 g/dl and serum creatinine concentration was 11.89 mg/dl. There was a significantrelationship between increasing of serum creatinine and decreasing of Hb (p = 0,013). Majority of CKD patients suffered from severeanaemia, there was relationship between increasing of serum creatinine and decreasing of Hb. To make routine blood tests and bloodsmears in CKD patients is needed, with large of samples to assess the relationship between Hb and serum creatinine.
AKURASI TES BACTIDENT AMINOPEPTIDA SE UNTUK MENGIDENTIFIKASI BAKTERI GRAM NEGATIF Ramla Tongko; Tenri Esa; Hardjoeno .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.990

Abstract

The information about Gram stain of a bactery can help the clinicians to choose good antimicrobial therapy because many of antimicrobial drugs have activity to positive or negative Gram bacterial selective. So it needed to have a method to early identificationGram stain of bactery, which can be faster, cheaper and more practice. Identification of Gram stain we can do with BactidentBactident aminopeptidase test, Gram stain test and culture test. The aimed of this study is to know the accuracy of bactident aminopeptidase test, Gram stain test and culture test. The aimed of this study is to know the accuracy of bactident aminopeptidase test with culture test in identify Gram negative bactery. A diagnostic test was done among 60 samples (pus and sputum) at Sub UnitInfection Disease Dr.Wahidin Sudirohusodo public Hospital of Makasar from March to July 2008. Bactident aminopeptidase test, Gramstain test and culture test were done to each sample. The data was analysed with 2 × 2 table. Accuracy of Bactident aminopeptidaseBactident aminopeptidase test on culture test were high sensitivity 61.11%, specificity 100.00%, positive predivtive value (PPV) 100.00% and negative predictivevalue (NPV) 63.16%. Sensitivity of Bactident aminopeptidase test on culture test were lower than sensitivity of Gram stain test onBactident aminopeptidase test on culture test were lower than sensitivity of Gram stain test on test on culture test were lower than sensitivity of Gram stain test on culture test (61.11% vs 83.33%). Specificity of Bactident aminopeptidase test on culture test were higher than specificity of Gram stainBactident aminopeptidase test on culture test were higher than specificity of Gram stain test on culture test were higher than specificity of Gram stain test on culture test (100.00% vs 79.19%). Accuracy of Bactident aminopeptidase test on Gram stain and culture test is high enough,Bactident aminopeptidase test on Gram stain and culture test is high enough, test on Gram stain and culture test is high enough, it can lead us conclude that Bactident aminopeptidase test can be usefull to the clinician for using the antimicrobial before culture testBactident aminopeptidase test can be usefull to the clinician for using the antimicrobial before culture test test can be usefull to the clinician for using the antimicrobial before culture test was provided.
ANALISIS CD4 PADA PENATALAKSANAAN PASIEN KOINFEKSI HIV-TB Nursin Abd. Kadir; Nurhayana Sennang; Hardjoeno .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.991

Abstract

The Human Immunodeficiency Virus (HIV) epidemic has increased the burden of tuberculosis (TB) among young adults, especially in populations where the prevalence of TB infection is high like Indonesia. TB is the most common opportunistic infection on HIV patients(50%) in developing countries. CD4 also known as T helper lymphocytes are coordinators of body`s immune response, and it has beendeclining in HIV infection and be worsened by TB infection. CD4 count are standard laboratory marker of disease progression to followupandprognosisantiretroviraltherapyinHIVinfection.AimstoseethevalueofCD4countofHIV-TBco-infectedpatientsbeforeandafterantituberculosistogetherwithantiretroviraltherapy.AlongitudinalstudywasconductedbycollectingsecondarydatafromthemedicalrecordandtheresultsofClinicalPathologyLaboratoryofHIV-TBco-infectedpatientsatWahidinSudirohusodoHospitalperiodJuly2007–August2008.DatawereanalyzedbyWilcoxonSignedRankTestandMannWhitneyTestwitha=0.05.Totalsamples(n)were20patients(14patientswithcontinoustherapyand6uncontinoustherapy).WefounddifferentmeansbetweenCD4countbeforeandafterTherapy.CD4countbeforetherapywas71.15±81.04andaftertherapywas114.95±109.71(p=0.089)withWilcoxonSignedRankTest.Theanalyzedwerecontinuedbydividedsamplesincontinousgroupcomparedwithuncontinousgrouptherapy.TheresultshowedtheCD4alterationincontinousgroupwas853.8%anduncontinousgroupwas-56.6%wherep=0.000(MannWhitneytest).CD4counthasincreasedsignificantlyinHIV-TBco-infectedpatients8.5foldfromCD4baselinewithcontinoustherapyandtheuncontinous one has decreased 0.5 fold from CD4 baseline.
NILAI UREUM, KREATININ, DAN PENYINGKIRAN KREATININ DI PENDERITA PENYAKIT GINJAL MENAHUN (KRONIK) I. Ismail; Mutmainnah .; Hardjoeno .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 13, No 3 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v13i3.910

Abstract

Chronically Kidney Disease (CKD) is still a health problem with a high incidence, complex aetiology, and often without complaintsor clinical symptoms except in the terminal stadium. To know the distribution of CKD according to age, sex group, aetiology, determiningstage of CKD based on creatinine clearance value, a retrospective study was conducted on 88 patients of CKD at Wahidin SudirohusodoPublic Hospital of Makassar from January to December 2004. The diagnosis of CKD was based on serum ureum value (ModifiedBarthelot), serum creatinine value (Jaffe) and creatinine clearance value (Cockroft Gault). Chronically Kidney Disease was found morefrequent in men 59.09% (52 of 88). The peak age prevalence was between 50 to less than < 60 years 29.55%. The eldest patient was 83years while the youngest was 16 years. The most fragment aetiology of CKD was hypertension 18.18% (16 of 88). Based on creatinineclearance values the medium group, severe group, and renal failure group were the age group respectively 3.41%, 19.32% and 77.27%.The prevalence of CKD is found more frequent in 50 less than 60 years of age. Hypertension seems to be the more predominant aetiology,and staging based on creatinine clearance value showed that most patients were in the renal failure group.
EVALUASI AKTIVITAS TRANSAMINASE, DAN KADAR BILIRUBIN PADA PENDERITA VIRUS HEPATITIS B DAN C Yosepin .; Benny Rusli; Hardjoeno .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.996

Abstract

Hepatitis virus replicates only in hepatocyte. In hepatocyte injury, transaminase enzymes which normally intracellularly will transform to the blood circulation. And there are variation of increasing transaminase activities in HBV and HCV. To evaluatetransaminase activities, and bilirubin level in patients with HBV and HCV. Study design was cross sectional of 76 patients with HBVand HCV, data from medical record at Wahidin Sudirohusodo Hospital, Makassar, from January 2006 until July 2008 period. Datawas analyzed with Mann Whitney test using SPSS. windows versi on 12. of 76 patients with HBV and HCV were found 59 Men and 17women, age 15–72 years old. In there were no statistical significance between transaminase activites, bilirubin direct,indirect (p > 0.05),but there was significance difference of total bilirubin between HBV and HCV (p < 0.05). There were no statistical difference of AST,ALT, bilirubin direct and bilirubin indirect between HBV and HCV. Injury hepatocelluler because of HBV and HCV can not be identifiedjust by transaminase activities and bilirubin level, but there are other factor that influence.
GAMBARAN FUNGSI HATI DAN GINJAL PADA PENDERITA MALARIA Darmawaty .; Fitriani M; Ruland DN Pakasi; Hardjoeno .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 1 (2008)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i1.950

Abstract

the prevalence of malaria disease is still high in Indonesia. It requires to be diagnosed and handled quickly to decrease themorbidity and the mortality. the purpose of this study was to evaluate the results liver and kidney function tests of malaria patients forcomplications and to know the etiology. A cross sectional method was used for this study by using medical record of malaria patientsthat at Wahidin Sudirohusodo hospital in Makassar during January to December 2006. Malaria was diagnosed based on pheripheralblood while the examination of Sgot, SgPt (DgKC method), ureum (Barthelots method) and creatinine (Jaffe's method) were doneby chemistry autoanalizer. the increasing concentration of Sgot was found on 14 patients (42.4%) which was consisted of high at 12patients, SgPt on 13 patients (39.4%), ureum on 2 patients (6.1%) and creatinine on 11 patients (33.3%). Plasmodium falciparum wasfound on 18 patients (54.5%) and Plasmodium vivax on 15 patients (45.5%). the Sgot, SgPt, ureum, and creatinine concentrationsmostly exist in normal range, this indicated that liver and kidney damage was still mild. the etiology of malaria disease was Plasmodiumfalciparum and Plasmodium vivax, it was found from examination of pheripheral blood. there was no significant different in this studyfor the increasing of Sgot, SgPt, ureum and creatinine concentrations based on plasmodium etiology.
Co-Authors AA Mappahya Benny Rusli Darmawaty . Esa, Tenri Fitriani M I Patellongi I. Ismail IA Hutagalung M Arif Mutmainnah . Nurhayana Sennang Nursin Abd. Kadir R Pakasi Ramla Tongko Rosnety, . Ruland DN Pakasi Yosepin .