Shinta Shinta
Department Of Biology, Faculty Of Science And Technology, Maulana Malik Ibrahim Malang Islamic State University, Gajayana Street 50, Malang-East Java, Indonesia

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In vitro embryogenic callus induction of Carica pubescens Lenne and K.Koch using 2,4-D (2,4-Dichlorophenoxy Acetic Acid) and BAP (6-Benzylaminopurin) Shinta Shinta; Eko Budi Minarno; I’anatur Rofiqoh
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.952 KB) | DOI: 10.23869/49

Abstract

Carica pubescens Lenne & K. Koch has benefits as a producer of medicinal compounds. Propagation of C. pubescens is important to be done through in vitro embryogenic callus culture. This research aimed to determine the effect of 2,4-D growth regulators, 2,4-D and BAP interactions on the induction of embryogenic callus C. pubescens Lenne & K. Koch. This research is experimental, using a completely randomized design (CRD) with 25 treatments and 5 replications. Embryonic callus was induced by various concentration of 2,4 D (0 mg/L, 2.5 mg/L, 5 mg/L, 7.5 mg/L, and 1 mg/L) in combination with BAP (0 mg/L, 2.5 mg/L, 5 mg/L, 7.5 mg/L, and 1 mg/L). Data were analyzed using two-way ANOVA, followed by the Duncan Multiple Range Test (DMRT) (p>0.05). The result showed that the addition of 2,4 D and BAP as a growth regulator gave an effect on embryonic callus. Concentration of 7.5 mg/L 2,4 D and 0.5 mg/L BAP in callus 19 days after planting showed the most effective interaction of both inductors. The combination produced callus fresh weight at 1.09 g and 99% in percentage of explant callus area. Morphological and anatomical observations showed the characteristic of embryogenic callus as indicated by yellowish, textured callus friable, having a large core and containing starch grains also plastids.
Pendampingan Ibu-Ibu PKK Kecamatan Lowokwaru Malang melalui Pelatihan Pembuatan Pestisida Nabati sebagai Pengendalian Hama Sayuran Hidroponik Liliek Harianie; Shinta Shinta; Lila Biarrohmah; Lina Hidayatur Rohmah; Widya Maslahah
Jurnal Pengabdian Pada Masyarakat Vol 5 No 1 (2020)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30653/002.202051.274

Abstract

GUIDING OF PKK MOTHERS IN LOWOKWARU MALANG DISTRICT THROUGH TRAINING FOR MAKING VEGETABLE PESTICIDES AS A CONTROL OF HYDROPONIC VEGETABLE PEST. In general, chemical pesticides are used to eradicate pests in vegetables. That is because many chemical pesticides are sold in the market and very effective in eradicating pests. The users of chemical pesticides do not understand, if the use of chemical pesticides for a long time and continuously will be very dangerous. Steps that can be taken to prevent the dangers of chemical pesticides is using natural or vegetable pesticides. Vegetable pesticides are more environmentally friendly, the prices are much cheaper, and not dangerous, and the manufacturing process is very easy. The social role of PKK mothers is carried out to improve the activities of coaching, education, training, and counseling. Therefore, it is necessary to conduct socialization and training in the manufacture of plant-based pesticides for PKK mothers in Lowokwaru District, Malang. The methods used include counseling through lectures, direct practice of making vegetable pesticides, and evaluating the process of making vegetable pesticides. The results of a survey of partners showed that 63% of PKK present did not know about vegetable pesticides. In addition, 81% of partners before participating in training activities had never been aware of the process of making vegetable pesticides. Around 90% of partners are known to have never participated in training in making vegetable pesticides. After participating in training and practice in making vegetable pesticides, it is known that 100% of the community service partners have stated that they can increase their knowledge, skills and practice by making vegetable pesticides at home.