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All Journal Jurnal Biogenerasi Journal Of Agriculture Sciences (Agrosci)
Priyambodo Priyambodo
University of Lampung
Agriculture, Biological Sciences & Forestry Education Languange, Linguistic, Communication & Media Other

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Learning RNA Isolation on Oral Swab Sample on Bats in Bandar Lampung Urban Areas Elfita Nova Yunior; Elly Lestari Rustiati; Enny Saswiyanti; Emantis Rosa; Priyambodo Priyambodo; Eko Agus Srihanto; Dian Neli Pratiwi
Jurnal Agrosci Vol 3 No 4 (2026): Vol 3 No 4 March 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i4.1166

Abstract

Background. Bats are recognized as important reservoir hosts for various zoonotic viruses, including coronaviruses, posing potential public health risks, particularly in urban environments where human–animal interactions are frequent. Aims. This study aimed to evaluate the effectiveness of RNA isolation from oral swab samples of bats captured in urban areas of Bandar Lampung, Indonesia, as a preliminary step for molecular detection of viral pathogens. Methods. Sampling was conducted using mist nets in the University of Lampung area, followed by oral swab collection and preservation in viral transport medium. RNA isolation was performed through four main stages: lysis, binding, washing, and elution. The quality of RNA was assessed using agarose gel electrophoresis, while the quantity was measured using a Qubit Fluorometer. Result. A total of six bats were successfully sampled, consisting of Cynopterus brachyotis and Cynopterus sphinx. Qualitative analysis revealed a single thin, luminescent band in all samples, indicating suboptimal RNA integrity. Quantitative results showed RNA concentrations ranging from 0.58 to 2.03 ng/µL, with three samples falling within the acceptable range (1.8–2.0 ng/µL), suggesting adequate RNA purity. Variations in RNA quality and concentration were likely influenced by factors such as incubation duration and sample handling, potentially leading to contamination or degradation. Conclusion. In conclusion, RNA isolation from bat oral swabs in urban areas is feasible; however, protocol optimization is necessary to improve RNA integrity and yield. High-quality RNA is essential for reliable downstream molecular analyses, including viral detection and surveillance of zoonotic diseases
Detection of Salmonella spp. Contamination in Chicken Meat Samples Using the SNI ISO 6579-1:2017 Method: Application of Standard Microbiological Detection Procedures in Poultry Meat Testing Vika Aulia Royani; Dzulfikar Yusuf Priyanatha Priyanatha; Justin S Sitorus Sitorus; Ngakan Putu Agung Dharma Pratama Pratama; Annisa Nur Adhiak Adhiak; Ni’matul Aliyah Aliyah; Hendri Busman Busman; Priyambodo Priyambodo; Ahmad Ikhsanudin Ikhsanudin; Jani Master Master
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8187

Abstract

Salmonella spp. is a pathogenic bacterium responsible for foodborne diseases and is commonly found in poultry products, particularly chicken meat. This study aimed to detect and identify Salmonella spp. contamination in chicken meat samples tested at the Veterinary Public Health Laboratory (Kesmavet) DKI Jakarta and to evaluate the results as a basis for food safety assessment. The examination was conducted using a standardized microbiological method based on SNI ISO 6579-1:2017, which includes pre-enrichment, selective enrichment, isolation on selective media, biochemical testing, and serological confirmation using polyvalent O and H antisera. Identification was determined based on specific agglutination reactions between bacterial antigens and antisera. The results demonstrated that the applied procedure was able to accurately detect the presence of Salmonella spp. in accordance with laboratory standards. Routine microbiological surveillance is essential to control contamination risks and improve the safety of animal-derived food products in order to protect public health.
Oral Swab-Based DNA Extraction Of Cynopterus Brachyotis: An Initial Step For Species Confirmation Nabila Aulia Rhamadaningtyas; Elly Lestari Rustiati; Priyambodo Priyambodo; Eko Agus Srihanto; Enny Saswiyanti; Dian Neli Pratiwi; Alvin Wiwiet Susanto
Jurnal Agrosci Vol 3 No 3 (2026): Vol 3 No 3 January 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i3.1025

Abstract

Background. Identifying bat species based on morphological characteristics often faces challenges due to character overlap among species, especially within cryptic species groups. Cynopterus brachyotis is one of the fruit bats with high genetic diversity but relatively uniform morphology. Aims. Therefore, a molecular approach is needed to support accurate species confirmation. Under the HETI Research Grant of Innovation and Collaboration Batch 3- II Year 2025, this study aims to obtain genomic DNA from C. brachyotis bats caught at the Lampung Disease Investigation Center as the initial step of molecular species confirmation. Methods. Samples were obtained by taking oral swabs on bat individuals caught using mist nets. DNA extraction is performed using silica membrane-based methods with commercial kits. Quantitative evaluation of DNA was performed using a Qubit Fluorometer, while qualitative evaluation was performed by agarose gel electrophoresis. Conclusion. The results showed that the DNA concentration was in the low range (0.1–0.7 ng/μL), with a DNA band appearing very thin on electrophoresis. Implementation. Nevertheless, the quantification results confirm that DNA was successfully extracted and remains suitable for advanced molecular analysis, with optimization at the DNA amplification stage.
Learning Bats Handling for Oral Swab Sampling Sevira Nur Azmi; Elly Lestari Rustiati; Priyambodo Priyambodo; Enny Saswiyanti; Eko Agus Srihanto; Dian Neli Pratiwi; Jani Master; Waryoko Susandi
Jurnal Agrosci Vol 3 No 4 (2026): Vol 3 No 4 March 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i4.1154

Abstract

Background. The only flying mammals, bats play an essential role in their natural habitat. Based on the type of food, bats can be divided into two groups: fruit-eating bats and insect-eating bats. Bats rank second-highest in species diversity, with 1,439 worldwide and 239 in Indonesia. Known as reservoirs of viruses, including coronaviruses. Bats are suspected to be related to COVID-19. Aims. Under the Research Innovation and Collaboration Program - Higher Education for Technology and Innovation Project (HETI) University of Lampung 2024-2025, and in collaboration with the Lampung Disease Investigation Centre, learning the procedures for handling bats properly so as not to physically harm bats in oral swab sampling and species recognition in Braja Harjosari, directly next to Way Kambas National Park was done. The life-trapping technique uses a mist net. Methods. Bat handling for taking an oral swab was carried out using the pinch grip method, holding both bat arms backwards with the thumb and middle finger, with the bat positioned facing upwards. Conclusion. The bat's mouth is then blown open, and a cotton swab is gently inserted into the bat's mouth. Afterwards, the bat is rested and given a drink/water and released into nature. Oral swab samples from 10 individual bats, fruit-eating bats, Cynopterus brachyotis (n = 8), Cynopterus horsfieldii (n = 1), and an insect-eating bat, Scotophilus kuhlii (n = 1).
RNA Isolation from Oral Swab Sample On Bats of Rural Area, Braja Harjosari, East Lampung Vidyanti Kurniasih; Elly Lestari Rustiati; Priyambodo Priyambodo; Eko Agus Srihanto; Enny Saswiyanti; Dian Neli Pratiwi
Jurnal Agrosci Vol 3 No 3 (2026): Vol 3 No 3 January 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i3.1161

Abstract

Background. Bats are considered animals that carry infectious diseases, are known to be reservoirs of viruses, and are suspected of causing disease outbreaks. The World Health Organization stated that this disease outbreak is caused by Coronavirus Disease 2019 (COVID-19), which is caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). Aims. Under the Research Innovation and Collaboration Program - Higher Education for Technology and Innovation Project (HETI) and in collaboration with the Lampung Disease Investigation Center, a preliminary study on EID detection on bats based on Predict Protocols was done to identify the presence of coronavirus on bats, including rural areas, Braja Harjosari, East Lampung. It is located next to Way Kambas National Park. Methods. Oral swab samples were taken from ten bats, 3 species: Cynopterus brachyotis and Cynopterus horsfieldi, and Scotophilus kuhlii. RNA extraction was conducted by Predict Protocols, which has four stages: lysis, binding, washing, and elution. Conclusion. Two samples showed thin-band luminescence. While in the quantity test, there are three samples of good concentration results between 1.8 and 2.0.
Co-Authors Ahmad Ikhsanudin Ikhsanudin Alvin Wiwiet Susanto Annisa Nur Adhiak Adhiak Dian Neli Pratiwi Dian Neli Pratiwi Dian Neli Pratiwi Dzulfikar Yusuf Priyanatha Priyanatha Eko Agus Srihanto Elfita Nova Yunior Elly Lestari Rustiati Emantis Rosa Enny Saswiyanti Enny Saswiyanti Hendri Busman Busman Jani Master Jani Master Master Justin S Sitorus Sitorus Nabila Aulia Rhamadaningtyas Ngakan Putu Agung Dharma Pratama Pratama Ni’matul Aliyah Aliyah Sevira Nur Azmi Vidyanti Kurniasih Vika Aulia Royani Waryoko Susandi