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All Journal Journal Of Agriculture Sciences (Agrosci)
Dian Neli Pratiwi
Institute Technology of Sumatra
Agriculture, Biological Sciences & Forestry

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DNA Extraction of Cynopterus brachyotis of Labuhan Ratu VII Based on Column Method in Supporting its Molecular Species Confirmation Annisa Lidya Maharani; Elly Lestari Rustiati; Priyambodo Priyambodo; Eko Agus Srihanto; Enny Saswiyanti; Dian Neli Pratiwi; Alvin Wiwiet Susanto
Jurnal Agrosci Vol 3 No 1 (2025): Vol 3 No 1 September 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i1.1043

Abstract

Background. Bats (Chiroptera) are true flying mammals and nocturnal. Ecologically, bats play important roles as pollinators, seed dispersers, guano producers, biocontrol, and hosts a range of viruses. Cynopterus brachyotis belongs to fruit-eating bats and is considered morphologically cryptic with C. sphinx. Aims. To support morphological identification, molecular species confirmation needed to be conducted. DNA extraction, as an initial step, plays a crucial role in molecular analysis. Methods. The quality and quantity of extracted DNA determine the success rate of sequencing for species confirmation in bats. In this study, oropharyngeal samples were used. Result. The research procedures included bat capture and DNA oropharyngeal swab sample collection, sample preparation, DNA extraction using a silica column-based commercial kit, DNA concentration measurement using a Qubit assay, and electrophoresis. Conclusion. The Qubit assay showed DNA concentrations of >1 ng/µL, while electrophoresis did not reveal bright DNA bands.
Learning Bats Handling for Oral Swab Sampling Sevira Nur Azmi; Elly Lestari Rustiati; Priyambodo Priyambodo; Enny Saswiyanti; Eko Agus Srihanto; Dian Neli Pratiwi; Jani Master; Waryoko Susandi
Jurnal Agrosci Vol 3 No 4 (2026): Vol 3 No 4 March 2026
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i4.1154

Abstract

Background. The only flying mammals, bats play an essential role in their natural habitat. Based on the type of food, bats can be divided into two groups: fruit-eating bats and insect-eating bats. Bats rank second-highest in species diversity, with 1,439 worldwide and 239 in Indonesia. Known as reservoirs of viruses, including coronaviruses. Bats are suspected to be related to COVID-19. Aims. Under the Research Innovation and Collaboration Program - Higher Education for Technology and Innovation Project (HETI) University of Lampung 2024-2025, and in collaboration with the Lampung Disease Investigation Centre, learning the procedures for handling bats properly so as not to physically harm bats in oral swab sampling and species recognition in Braja Harjosari, directly next to Way Kambas National Park was done. The life-trapping technique uses a mist net. Methods. Bat handling for taking an oral swab was carried out using the pinch grip method, holding both bat arms backwards with the thumb and middle finger, with the bat positioned facing upwards. Conclusion. The bat's mouth is then blown open, and a cotton swab is gently inserted into the bat's mouth. Afterwards, the bat is rested and given a drink/water and released into nature. Oral swab samples from 10 individual bats, fruit-eating bats, Cynopterus brachyotis (n = 8), Cynopterus horsfieldii (n = 1), and an insect-eating bat, Scotophilus kuhlii (n = 1).
Co-Authors Alvin Wiwiet Susanto Annisa Lidya Maharani Eko Agus Srihanto Elly Lestari Rustiati Elly Lestari Rustiati Enny Saswiyanti Jani Master Priyambodo Priyambodo Priyambodo Priyambodo Sevira Nur Azmi Waryoko Susandi