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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Penelitian Pascapanen Pertanian BERITA BIOLOGI Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research)
Endang S Rahayu
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Public Health Social Sciences

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KARAKTERISASI GEN PENYANDI PEDIOSIN PAF-11 PADA Pediococcus acidilactici F-11 [Characterization of the Pediocin PaF-11 Encoding Gene in Pediococcus acidilactici F-11] Marwati, Tri; Richana, Nur; Harmayani, Eni; Rahayu, Endang S
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.137 KB) | DOI: 10.14203/beritabiologi.v11i2.495

Abstract

Pediocin PaF-11 is a ribosomally synthesized antimicrobial peptide produced by Pediococcus acidilactici F-11. The objectives of this research is to find out the location and the nucleotide sequence of gene, which is involved in the production of pediocin PaF-11. Results showed that the pediocin PaF-11 from the cured cell of P. acidilactici F-11 loss the activity, suggested that the pediocin PaF-11 gene was carried in the plasmid. Agarose gel electrophoresis of P. acidilactici F-11 plasmid DNA with marker λDNA/HindIII showed that pediocin PaF-11 gene was carried in 12 kb plasmid. Amplification pediocin PaF-11 gene from P. acidilactici F-11 showed that uncured P.acidilactici F-11 culture contain plasmid DNA, indicated by amplification of the papA gene (256 bp). Cured P. acidilactici F-11 culture, plasmid eliminated, indicted by no aplicon DNA detected. This result also suggested that pediocin PaF-11 gene in P. acidilactici F-11 was carried in plasmid. Nucleotide of pediocin PaF-11 encoding gene was sequenced The alignment of that nucleotide sequence showed that pediocin PaF-11 encoding gene have the same sequence with pediocin PA.1 encoding gene in P. acidilactici PAC1.0 and P. acidilactici K10 and pediocin AcH encoding gene in P. acidilactici LB 42-923 and P .parvulus ATO77, and pediocin CP2 in P. acidilactici MTCC 5101.
Supplementation of Lactocbacillus acidophilus SNP-2 Into Tape and its Effect to the Volunteer Endang S Rahayu; Siti N Purwandhani
Jurnal Teknologi dan Industri Pangan Vol. 15 No. 2 (2004): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Functional food is defined as any potentially healthful food or food ingredient that may provide a health benefit beyond the traditional nutrients it contains. Many researches have been conducted on the health benefit of probiotic (life bacterial cells), one of the ingredient of functional foods. One of the potential bacteria used for probiotic agent and also involved in traditional fermented foods are lactic acid bacteria (LAB). Previous research showed that Lactobacillus acidophilus SNP-2 isolated from faecal material of healthy infant is resistant to acid and bile salt, and has an antagonistic effect against several enteric bacterial pathogens. The objective of this research was to study the effect of L. acidophilus SNP-2 as probiotic agent to the health benefits. These bacteria were supplemented into tape ketan (fermented sticky rice), the indigenous Indonesian fermented food. Tape ketan was chosen as the carrier of probiotic biomass based on the high population of LAB in this product, i.e., 1.3 x 108 CFU/g. Addition of L. acidophilus SNP-2 biomass prior to fermentation of tape ketan resulted in a higher total of LAB cells, i.e. 2.1 x 109 CFU/g compared to the amount of 1.5 x 108 CFU/g when the addition was done after fermentation. Consumption of tape ketan containing probiotic agent by the volunteers increased the population of lactobacilli (from 1.7x107 CFU/g to 9.9x107 CFU/g) and decreased the population of enterobacteriacea (from 5.4x109 CFU/g to 4.4x108) in their faecal material. This phenomenon revealed that probiotic agent was able to colonize and inhibit the growth of enterobacteriaceae in the gastrointestinal tract. The result implied that tape ketan can be used as a carrier for probiotic agent and it can be categorized as functional food. Key words : Tape ketan, lactobacillus acidophilus, probiotic.
Viability and Stability of Lactobacillus Plantarum Mut 7 FNCC 250 Suplemented in Papaya-Pineapple Juice During Storage Sri Hartati; Eni Harmayani; Endang S Rahayu; Tyas Utami
Jurnal Teknologi dan Industri Pangan Vol. 14 No. 2 (2003): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Lactobacillus plantarum Mut7 FNCC 250 is an indigenous bacterium isolated from fermented food (“gatot”) and a potential agent to reduce cholesterol. Study the on aplication of the bacterium as probiotic agent to food stuff is needed. The purpose of the study was to prepare formula of papaya-pineapple juice as probiotic carrier and to study the viability and capability of Lactobacillus plantarum Mut7 FNCC 250 to assimilate cholesterol during three months storage. Fruits juice was prepared with different formula and organoleptically tested. Selected juice formula was supplemented with Lactobacillus plantarum Mut7 FNCC 250 and stored in cool room (4-5 oC). During three month storage, the value of pH the product, cell viability and ability to assimilate cholesterol were evaluated. The result indicated that acceptable formula was papaya juice added with 25 % pineapple. Viability of Lactobacillus plantarum Mut7 FNCC 250 supplemented in papaya- pineapple juice was stable (decrease of < 1 log cycle), while the pH of the juice decreased after 3 month storage. The ability of Lactobacillus plantarum Mut7 FNCC 250 to assimilate cholesterol was stable within 2 months storage, but decreased after 3 months. Key words : Viability, sta, Lactobacillus plantarum, probiotic, papaya-pineapple juice.
Extraction and Characterization of Bacteriocin Produced by Leuconostoc mesenteroides SM 22 Darnawan Ari Nugroho; Endang S Rahayu
Jurnal Teknologi dan Industri Pangan Vol. 14 No. 3 (2003): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Bacteriocin produced by lactic acid bacteria has potential as food biopreservative due to their capability to control spoilage and pathogenic food borne bacteria. Previous studies showed that extraction of bacteriocin produced by Leuconostoc mesenteroides SM 22 using adsorption-desorption method was not optimal. The objectives of this research were (1) to increase the effectiveness of bacteriocin extraction using adsorption-desorption method by the addition of heated biomass of Leuconostoc SM 22 in various concentration during adsorbtion (2) to characterize the bacteriocin of Leuconostoc mesenteroides SM 22 on it's stability during heat treatment, during cool storage and it's spectrum activity againts pathogenic bacteria. Result of this research showed that bacteriocin activity obtained from extraction with no addition of heated biomass was 1000 AU/ml, while by addition of heated biomass of 2 to 3 times of original concentration (OD) were 2000 AU/ml. Therefore it was suggested that addition of heated biomass of Leuconostoc mesenteroides SM 22 during adsorption-desorption with 2 times of original concentration (OD) was able to increase the bacteriocin obtained. Bacteriocin with original activity of 2000 AU/ml, was stable (no reduction activity) after heated at 100oC for 30 minutes, but slightly decrease after heated at 121oC for 5 minutes and 121oC for 15 minutes, that were 1600 AU/ml and 800 AU/ml respectively. Bacteriocin of Leuconostoc mesenteroides SM 22 was stable during 8 weeks storage at refrigerator (4oC), freezer -20oC and -40oC. This bacteriocin has a wide spectrum of activity showed by it's ability to inhibit the growth of Listeria monocytogenes, Staphylococcus aureus, Salmonella thypimurium, Vibrio parahaemolyticus, Shigella and psychrophilic bacteria isolated from milk and isolated from meat. Key words : Bacteriosin, leuconostoc mesenteroides.
Teknik Produksi Dan Purifikasi Pediosin Paf-11 Dari Pediococcus Acidilactici F-11 Tri Marwati; Nur Richana; Eni Harmayani; Endang S Rahayu
Jurnal Penelitian Pascapanen Pertanian Vol 9, No 1 (2012): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v9n1.2012.11-17

Abstract

Pediosin PaF-11 dari Pediococcus acidilactici F-11 berpotensi sebagai pengawet pangan karena kemampuannya dalam mengendalikan pertumbuhan bakteri pembusuk pangan. Efektivitas purifikasi diperlukan dalam aplikasi pediosin PaF-11 pada industri pangan. Untuk itu dilakukan penelitian yang bertujuan untuk mengetahui aktivitas pediosin PaF-11 selama proses inkubasi sel P. acidilactici F-11 dan meningkatkan efektivitas purifikasi pediosin PaF-11. Perlakuan yang dicobakan pada proses purifikasi yaitu adsorpsi dan desorpsi pada pH yang bervariasi dan penambahan biomassa sel mati dari P. acidilactici F-11 pada konsentrasi yang bervariasi selama proses adsorpsi. Aktivitas antibakteri pediosin PaF-11 diuji dengan metode difusi agar menggunakan bakteri indikator Lactobacillus pentosus LB42. Hasil penelitian menunjukkan bahwa pediosin PaF-11 yang diproduksi dengan menggunakan kultur awal P. acidilactici F-11 sebanyak 10% dengan lama inkubasi 16 jam mempunyai aktivitas 2000 AU/ml. Pediosin PaF-11 yang dihasilkan P. acidilactici F-11 dengan kultur awal 1% dan purifikasi pada pH adsorpsi pH 6,5 dan pH desorpsi 2,0 memiliki aktivitas tertinggi yaitu 1500AU/ml, dibandingkan perlakuan pH yang lain. Aktivitas pediosin PaF-11 yang dihasilkan dari proses purifikasi tanpa penambahan biomassa sel mati yaitu 1500AU/ml, sedangkan dengan penambahan biomassa sel mati 3, 6 dan 11 kali dari konsentrasi awal menjadi 3000AU/ml. Hal ini berarti bahwa dengan penambahan biomassa sel mati P. acidilactici F-11 dengan 3 kali konsentrasi awal mampu meningkatkan pediosin PaF-11 yang diperoleh.
Mekanisme Awal dan Aplikasi Antibakteri Pediosin PaF-11 Sebagai Pengawet Tahu Tri Marwati; Nur Richana; Irinne D.P; Eni Harmayani; Endang S Rahayu
Jurnal Penelitian Pascapanen Pertanian Vol 9, No 2 (2012): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v9n2.2012.54-62

Abstract

Pediosin PaF-11 dari Pediococcus acidilactici F-11 merupakan peptida antibakteri yang aktif pada kisaran pH luas dan stabil pada perlakuan suhu tinggi dan rendah sehingga potensial digunakan sebagai pengawet tahu. Penelitian ditujukan untuk mengetahui mekanisme awal penghambatan pediosin PaF-11 sebagai antibakteri dan aplikasinya sebagai pengawet tahu. P. acidilactici F-11 dan Lactobacillus pentosus LB42 berturut turut digunakan sebagai bakteri penghasil dan indikator uji aktivitas pediosin PaF-11. Mekanisme awal kerja penghambatan pediosin PaF-11 ditentukan berdasar kajian pengaruh gadolinium (Gd3+) terhadap aktivitas pediosin PaF-11, kadar Gd3+ pada dinding sel dan morfologi sel indikator. Uji aktivitas pediosin PaF-11 dilakukan dengan metode difusi agar sumur. Aplikasi pediosin PaF-11 dan bakteriosin komersial nisin dilakukan terhadap tahu produksi CV. Kitagama Yogyakarta. Perlakuan meliputi perendaman tahu dalam larutan nisin (500 IU/g, 1000 IU/g dan 2000 IU/g) dan larutan pediosin PaF-11 30 AU/g. Setelah perendaman, dilanjutkan dengan pasteurisasi pada suhu 90oC selama 10 menit dan penyimpanan pada suhu 4oC selama 16 hari. Tahu tanpa perendaman dalam larutan nisin dan pediosin PaF-11 digunakan sebagai kontrol. Hasil penelitian mengindikasikan bahwa mekanisme awal penghambatan pediosin PaF-11 sebagai antibakteri yaitu melalui interaksi pediosin PaF-11 yang bermuatan positif dengan asam teikoat dan asam lipoteikoat yang bermuatan negatif. Hasil penelitian menunjukkan bahwa total bakteri awal pada tahu kontrol adalah 105 dan mengalami kenaikan menjadi 108 setelah disimpan. Penambahan larutan nisin dengan konsentrasi minimal 500 IU/g mampu menghambat populasi bakteri pada tahu sebesar 2 log cycle sedangkan larutan pediosin PaF-11(30 AU/g) mampu menghambat populasi bakteri pada tahu sebesar 0,5 log cycle.
DIET BERAS MERAH DAN BERAS PUTIH PRATANAK PADA TIKUS HIPERGLIKEMIA Dita Kristanti; Viera Nu’riza Pratiwi; Endang S Rahayu; Mary Astuti
Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research) Vol. 44 No. 2 (2021): PGM VOL 44 NO 2 TAHUN 2021
Publisher : Persagi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/pgm.v44i2.4421

Abstract

ABSTRACT Hyperglycemia is a carbohydrate metabolism disorder causing diabetes. The difference in glycemic index (GI) in red rice and white rice causes different effects on blood glucose. Precooked rice processing causes starch retrogradation which can form Resistant Starch (RS) thereby lowering the GI of rice. Resistant starch is fermented into Short Chain Fatty Acids (SCFAs) and affects the colonic microbiota population. This study aims to determine the effect of precooked red rice and precooked white rice consumption on fasting blood glucose concentration, Ferric Reducing Ability on Plasma (FRAP) concentration, Escherichia coli, and Lactobacillus populations, and SCFAs digesta in hyperglycemic Wistar rats. This study was conducted using 24 male Wistar rats aged 8 weeks with a bodyweight of 200-220 grams. The rats were divided into 4 treatment groups: healthy (S), hyperglycemic (H), a hyperglycemic diet of precooked red rice (H+BMP), and hyperglycemic rats of precooked white rice diet (H+BPP). Consumption of precooked red rice (BMP) for 6 weeks was shown to reduce fasting blood glucose concentration (57.95%), while consumption of cooked white rice (BPP) actually increased fasting blood glucose concentration (4.16%) in hyperglycemic rats. Consumption of BMP also resulted in higher blood antioxidant capacity than BPP. However, consumption of BMP and BPP had no significant effect on the E. coli population, Lactobacillus population, and SCFAs digesta levels. ABSTRAK Hiperglikemia adalah suatu tanda gangguan metabolisme karbohidrat yang menyebabkan penyakit diabetes. Perbedaan Indeks Glikemik (IG) pada beras merah dan beras putih menyebabkan perbedaan efek pada glukosa darah. Pengolahan beras secara pratanak menyebabkan retrogradasi pati yang dapat membentuk Resistant Starch (RS) sehingga menurunkan IG pada beras. Resistant starch difermentasi menjadi Short Chain Fatty Acids (SCFAs) dan mempengaruhi populasi mikrobiota di kolon. Penelitian ini bertujuan untuk mengetahui pengaruh konsumsi beras merah dan beras putih precooked terhadap konsentrasi glukosa darah puasa, konsentrasi Ferric Reducing Ability on Plasma (FRAP), populasi Escherichia coli dan Lactobacillus, serta SCFAs digesta tikus Wistar hiperglikemia. Penelitian ini dilakukan menggunakan 24 ekor tikus putih Wistar jantan berumur 8 minggu dengan berat badan 200-220 gram. Tikus dibagi menjadi 4 kelompok perlakuan: sehat (S), hiperglikemia (H), hiperglikemia diet beras merah pratanak (H+BMP), dan tikus hiperglikemia diet beras putih pratanak (H+BPP). Konsumsi beras merah pratanak (BMP) selama 6 minggu terbukti menurunkan konsentrasi glukosa darah puasa (57.95%), sedangkan konsumsi beras putih pratanak (BPP) justru meningkatkan konsentrasi glukosa darah puasa (4.16%) pada tikus hiperglikemia. Konsumsi BMP juga menghasilkan kapasitas antioksidan darah yang lebih tinggi dibandingkan BPP. Namun, konsumsi BMP dan BPP tidak berpengaruh nyata terhadap populasi E. coli, populasi Lactobacillus, dan kadar SCFAs digesta. [Penel Gizi Makan 2021, 44(2):93-104]
Co-Authors Darnawan Ari Nugroho Dita Kristanti Eni Harmayani Irinne D.P Mary Astuti Nur Richana Pratiwi, Viera Nuriza Siti N Purwandhani Sri Hartati Tri Marwati Tri Marwati Tyas Utami