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Seleksi dan Pengujian Potensi Bakteri Indigenous Air Rendaman Kenaf (Hibiscus cannabinus L.) Sebagai Bakteri Selulolitik, Pektinolitik, dan Lignolitik Rahayu, Farida; Sudjindro, .; Budi, Untung Setyo
Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 2, No 2 (2010): Oktober 2010
Publisher : Balai Penelitian Tanaman Pemanis dan Serat (Balittas)

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Abstract

Serat sintetik yang selama ini banyak digunakan, dipandang tidak ramah lingkungan karena berpotensi men-jadi pencemar. Untuk itulah, inovasi untuk mendapatkan bahan yang lebih ringan, murah, dan ramah ling-kungan dikembangkan. Pemanfaatan bahan mentah berupa serat tanaman untuk bahan selain tekstil khu-susnya serat kenaf (Hibiscus cannabinus) banyak mendapat perhatian khusus dari berbagai kalangan. Tuju-an dari penelitian ini adalah untuk menyeleksi dan menguji potensi bakteri selulolitik, pektinolitik, dan lignoli-tik indigenous dari air rendaman kenaf sebagai sumber inokulum pada proses retting kenaf. Eksplorasi dila-kukan dengan mengambil sampel air rendaman kenaf di Asembagus. Isolasi bakteri dilakukan dalam media Nutrient Broth (NB) dan Nutrient Agar (NA) yang merupakan media umum untuk bakteri. Isolat bakteri ke-mudian dikulturkan dan dipelihara dalam medium selektif mengandung carboxyl metylcellulase (CMC), pek-tin, atau lignin. Waktu optimal pertumbuhan sel dan nilai indeks selulolitik, pektinolitik, dan lignolitik ditentu-kan berdasarkan nisbah antara diameter zona bening di sekitar koloni dengan diameter koloni bakteri. Hasil eksplorasi didapat 8 isolat bakteri yang berpotensi sebagai bakteri selulolitik, pektinolitik, dan lignolitik; 1 iso-lat berpotensi sebagai bakteri selulolitik dan lignolitik; dan 2 isolat berpotensi sebagai bakteri pektinolitik; ser-ta 1 isolat berpotensi sebagai bakteri selulolitik. Hampir semua isolat memiliki waktu optimal pertumbuhan pa-da jam ke-12–18 dengan jumlah sel 21,9–267 juta sel/ml pada suhu 37oC. Synthetic fiber has been widely used and considered as an environmentally unfriendly product because its po-tency as a contaminant. For this reason, innovation to get lighter, cheaper, and environmentally friendly ma-terials is developed. Utilization of plant fibers for raw materials other than textiles especially kenaf fiber (Hi-biscus cannabinus) has received special attention from various communities. The aim of this research was to isolate indigenous cellulolytic, pectinolytic, and lignolytic bacteria from water retting of kenaf for inoculum sources in retting process of kenaf. Exploration of bacteria was done in Asembagus by collecting retting wa-ter of kenaf. Isolate was done in common media for bacteria, i.e Nutrient Broth (NB) and Nutrient Agar (NA), then the isolate bacteria were selected in a selective medium contained carboxyl metylcellulase (CMC), pectin, or lignin. Determination of optimal time of cell growth and value of index cellulolytic, pectinolytic, and lignolytic activity based on the ratio between the diameter of clear zone around the colony and diameter of the bacterial colony. The isolates collected from this study were 12 numbers, consist of 8 isolates of bacteria with cellulolytic, pectinolytic, and lignolytic ability; 1 isolate of bacteria with cellulolytic and lignolytic ability; 2 isolates of pectinolytic bacterium; and 1 isolate as cellulolytic bacterium. Almost all isolates have optimal time of growth at 12th18th hour with number of cells between 21,9267 million cell/ml at 37oC.
Potensi Beberapa Isolat Bakteri Pelarut Fosfat Asal Lahan Tebu di Jawa Timur Berdasarkan Aktivitas Enzim Fosfatase Rahayu, Farida; Mastur, .; Santoso, Budi
Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 6, No 1 (2014): April 2014
Publisher : Balai Penelitian Tanaman Pemanis dan Serat (Balittas)

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Abstract

 Fosfor (P) merupakan hara esensial untuk pertumbuhan tanaman karena P berperan penting dalam banyak ak-tivitas metabolisme tanaman. Tanaman memperoleh P dari larutan tanah dalam bentuk anion. Namun, anion P sangat reaktif dan dapat mudah terikat oleh unsur Al, Fe, Mg, dan Ca. Dalam bentuk tersebut, P sangat tidak terlarut sehingga tidak tersedia bagi tanaman. Bakteri Pelarut Fosfat (BPF) berperan penting dalam meningkatkan ketersediaan P dalam tanah sehingga potensi BPF yang diisolasi dari lahan tebu perlu diidentifikasi. Kegiatan identifikasi potensi bakteri pelarut fosfat dilakukan mulai Januari–Desember 2012 di Laboratorium Bioprosesing Balai Penelitan Tanaman Pemanis dan Serat, Malang. Tujuan dari kegiatan ini adalah untuk melakukan eksplorasi bakteri pelarut fosfat dan seleksi berdasarkan kemampuan bakteri dalam melarutkan fosfat. Isolat dieksplorasi dari lahan tebu di Jawa Timur yaitu di Kabupaten Sidoarjo, Blitar, Kediri, Malang, Lumajang, Bondowoso dan Situbondo. Dari 65 isolat bakteri yang berhasil diisolasi, 22 isolat bakteri diantaranya berpotensi sebagai bakteri pelarut fosfat (BPF). Setelah dilakukan uji lebih lanjut, diperoleh 9 isolat unggul bakteri pelarut fosfat yaitu SD-10, Bl-1, KD-5, ML-2, LJ II-3 yang menunjukkan aktivitas fosfatase tinggi di hari pertama, sedangkan LJ I -3 dan BD-2 menunjukkan aktivitas fosfatase pada hari kedua dan SD-7 serta BL-4 termasuk dalam 9 besar isolat dengan diameter zona bening terbesar. Luas daerah zona bening secara kualitatif menunjukkan besar kecilnya kemampuan bakteri dalam melarutkan fosfat. Isolat BPF tersebut diharapkan dapat membantu memperbaiki ketersediaan P di tanah dan mampu memperbaiki kualitas pertumbuhan dan perkembangan tanaman tebu. Phosphorus (P) is an essential nutrient for plant growth, because it plays an important role in many metabolisms activities. Plants obtain P from soil solution as anion. However, phosphate anions are very reactive and can be immobilized through precipitation with Al, Fe, Mg, and Ca. In these form, phosphate is insoluble and unavailable to plants. Phosphate solubilizing bacteria (PSB) plays important role in dynamics and availability of P in soil. So, the potency of PSB isolates which were explored from sugarcane soil of East Java might be important to be identified. Identification based on activity of phosphatase enzyme was conducted from January–December 2012 in Bioprocessing Laboratory Indonesian Sweetener and Fiber Crops Research Institute, Malang. The aim was to explore and select PSB based on their ability to dissolve of P. Isolation of PSB was collected from sugar cane land of East Java included Sidoarjo, Blitar, Kediri, Malang, Lumajang, Bondowoso and Situbondo. Among 65 bacterial isolates, 22 bacterial isolates were potentially as PSB. After a further test, we obtained 9 isolate had high enzyme activities, ie. SD-10, BL-1, KD-5, ML-2 and LJ II-3 had phosphatase activity on the first day, whereas LJ I-3 dan BD-2 had an activity at the second day, while SD-7 and BL-4 had largest diameter of clear zones. Phosphate solubilizing bacteria isolate is expected to increase improve availability of P in the soil, quality and development of plants.
Isolation of Nitrogen-Fixing Bacteria from Rhizosphere of Sugarcane Plantation as Candidates for Biofertilizer Bin yahmed, Altayib; Mustafa, Irfan; Rahayu, Farida
The Journal of Experimental Life Science Vol. 15 No. 1 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.01.04

Abstract

Exploration of nitrogen-fixing bacteria as a sustainable alternative to conventional fertilizers is important in agriculture. The study aims to isolate and characterize the potential nitrogen-fixing bacteria (NFB) from soils of several sugarcane fields in East Java. Specifically, the bacteria were isolated from sugarcane fields in Blitar, Ngawi, and Glugur Malang. Soil samples were collected, and NFB were isolated using an Nfb medium. Next, the ability of these isolates to fix nitrogen in a liquid medium was evaluated by measuring the ammonium released after different incubation periods (24, 72, and 120 hours). The study obtained five potential isolates that grew well on the agar medium and changed the medium’s pH. Significant growth and pH increase at the liquid medium were observed across the isolates over time. This rise in pH was not always attributed to the ammonia production by those NFB. Only two isolates released ammonia at detectable concentrations as much as 1.13 mg.L-1 and 1.40 mg.L-1, respectively. Hence, isolates C and D were selected as potential NFB for application in replacing synthetic nitrogen fertilizer. Keywords: biofertilizer, environmental sustainability, nitrogen-fixing bacteria, rhizosphere.
Screening of LAB-Compatible Fungi and the Effect of Corncob Substrate Concentration on D(-) Lactic Acid Production using the Multiple Parallel Fermentation (MPF) Approach: Skrining Kapang yang Kompatibel dengan BAL serta Pengaruh Konsentrasi Substrat Tongkol Jagung terhadap Produksi Asam Laktat D(-) melalui Pendekatan Multiple Parallel Fermentation (MPF) Mufidah, Elya; Mifzal, Adib Maula; Veliska, Audrya Nasywa; Rahayu, Farida; Irawati, Maghfira Selia; Wibisono, Yusuf; Sugiarto, Yusron; Nurika, Irnia
Journal of Tropical Agricultural Engineering and Biosystems - Jurnal Keteknikan Pertanian Tropis dan Biosistem Vol. 13 No. 2 (2025): August 2025
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkptb.2025.013.02.02

Abstract

Biological production of D(-) lactic acid is being advanced as an eco-friendly alternative to petrochemical routes. This study optimizes D(-) lactic-acid production from corn cobs through Multiple-Parallel Fermentation (MPF), which combines a polysaccharide-degrading filamentous fungus with lactic-acid bacteria (LAB) in a single reactor. The novelty of the work lies in deploying a specific consortium of Aspergillus sp. and Leuconostoc mesenteroides for parallel fermentation of corn-cob biomass—a combination not previously reported. Three fungal isolates and three LAB strains were screened to identify the most compatible pairing. Fermentations were run at initial pH 7.0 and 39 °C with substrate concentrations of 3, 5 and 7% w/v. The 7% loading yielded the highest D(-) lactic-acid titer (14.01 % w/v), although differences among treatments were not statistically significant (p > 0.05). One-way ANOVA satisfied normality and homogeneity-of-variance assumptions, and linear regression indicated a moderate relationship between substrate concentration and lactic-acid yield (R² = 0.429). A D/L ratio exceeding 95:5 across all treatments confirmed selective D(-) isomer formation by L. mesenteroides. These results demonstrate the promise of MPF using local lignocellulosic waste as an efficient, sustainable route to D(-) lactic acid.