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All Journal Medical Journal of Indonesia Jurnal Riset Kimia YARSI Medical Journal
Asmah Rahmat
Department of Nutrition and Health Sciences, Faculty of Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor D.E
Chemistry Medicine & Pharmacology

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Cytotoxic effect of γ-sitosterol from Kejibeling (Strobilanthes crispus) and its mechanism of action towards c-myc gene expression and apoptotic pathway Endrini, Susi; Rahmat, Asmah; Ismail, Patimah; Taufiq-Yap, Y.H.
Medical Journal of Indonesia Vol 23, No 4 (2014): November
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.171 KB) | DOI: 10.13181/mji.v23i4.1085

Abstract

Background: This study aimed to analyze the cytotoxicity effect of γ-sitosterol isolated from “Kejibeling” (Strobilanthes crispus), a medicinal plant, on several cancer cell lines. The mechanisms of the effects were studied through the expression of cancer-caused gene, c-myc and apoptotic pathways.Methods: This in vitro study was done using human colon cancer cell lines (Caco-2), liver cancer cell lines (HepG2), hormone-dependent breast cancer cell lines (MCF-7) and the normal liver cell lines (Chang Liver). The cytotoxic effect was measured through MTT assay and the potential cytotoxic value was calculated by determining the toxic concentration which may kill up to 50% of the total cell used (IC50). Meanwhile, the cytotoxic mechanism was studied by determining the effect of adding γ-sitosterol to the c-myc gene expression by reverse transciptase-polymerase chain reaction (RT-PCR). The effect of γ-sitosterol through apoptotic pathway was studied by using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay.Results: γ-sitosterol was cytotoxic against Caco-2, HepG2, and MCF-7 with IC50-values of 8.3, 21.8, and 28.8 μg/mL, respectively. There were no IC50-values obtained from this compound against Chang Liver cell line. This compound induced apotosis on Caco-2 and HepG2 cell lines and suppressed the c-myc genes expression in both cells.Conclusion: γ-sitosterol was cytotoxic against colon and liver cancer cell lines and the effect was mediated by down-regulation of c-myc expression and induction of the apoptotic pathways.
CHARACTERISTIC OF APOPTOSIS AND EXPRESSION OF GENES-RELATED APOPTOSIS (c-myc, c-erb AND c-fos ONCOGENE) IN HeLa CELL LINES AFTER EXPOSURE BY NEEM (Azadirachta indica A.Juss) Arisanty, Dessy; Eshak, Zolkapli; Othman, Fauziah; Rahmat, Asmah; Akim, Abdah M.D.; -, Nurmawaty; M. Jalil, Suhaida.
Jurnal Riset Kimia Vol 1, No 2 (2008): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v1i2.35

Abstract

 ABSTRACT Azadirachta indica A. Juss is a medicinal plant commonly known as neem. The effect of neem leaves extract on cervical cancer cells, however, has never been studied. Due to the lack of information, this study was conducted to determine the effect of neem leaves extract on cervical cancer (HeLa) cell growth. In vitro cytotoxicity effect of ethanolic neem extract indicated the presence of cytotoxicity activity of the extract against HeLa cells with IC50 of  30.0 μg/mL. The morphological changes under confocal laser scanning microscope (CLSM) on HeLa cells were cell shrinkage and membrane blebbing. There were also cells with condensed nucleus and few cells have fragmented nucleus, and finally formed apoptotic body. Control cells showed a clear cytoplasm and centrally placed nucleus and no cells exhibited any apoptotic features. Appearance of apoptotic cells under scanning electron microscope (SEM) are indentations, blebs and hole on cell surface and disintegration of cell. The controls remained morphologically normal. Apoptotic features of the cells are widely seen with longer incubation time while 24 hours incubation time, it is scarcely seen. The RT-PCR product showed that the c-erb gene expression was expressed in both treated and untreated HeLa cells. Contrary, the the c-myc and c-fos oncogenes on  HeLa cells which exposed to A. indica  EtOH extract were significantly decreased.  Thus, the results from this study strongly suggest that the ethanolic extract of A. indica may contain bioactive compound(s) that caused cervical cancer cells, HeLa cell death by apoptosis mechanism and lead to succession of discovering new alternative treatment for cervical cancer.  Keywords : cytotoxic, apoptosis genes, oncogenes, neem
EFFECTS OF STROBILANTHES CRISPUS EXTRACT ON THE APOPTOTIC PATHWAY OF HUMAN LIVER CARCINOMA CELL LINES Endrini, Susi; Suherman, Suherman; Rahmat, Asmah; Ismail, Patimah; Taufiq-Yap, Y.H; Othman, Fauziah
Jurnal Kedokteran YARSI Vol 15, No 1 (2007): JANUARI - APRIL 2007
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.011 KB) | DOI: 10.33476/jky.v15i1.998

Abstract

Previous study has been shown the potential anticarcinogenic effect of Strobilanthes crispus, a plant native to countries from Madagascar to Indonesia, on human liver cancer (HepG-2) cell lines. The objective of the present study was to investigate the mechanism of anticarcinogenic effectof S.crispus extract through apoptotic pathway. Exposure of HepG-2 cells to S.crispus extract resulted in induction of apoptosis in a dose-dependent manner as measured by Fluoresence Microscopy and Confocal Laser Scanning Microscopy analysis. These findings provide important new insight into the possible molecular mechanisms of the anticancer activity of S.crispus.
Co-Authors Abdah M.D. Akim Dessy Arisanty Fauziah Othman Nurmawaty - Patimah Ismail Suhaida. M. Jalil Susi Endrini Syamsul Arifin Taufiq-Yap, Y.H Y.H. Taufiq-Yap Zolkapli Eshak