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Agus Subiwahjudi
Fakultas Kedokteran Gigi Universitas Airlangga
Dentistry

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Daya Hambat Aktivitas Enzim Glukosiltransferase (Gtf) Streptococcus mutans Oleh Ekstrak Temulawak (Curcuma Xanthorrhiza Roxb.) A. Amanda; Sri Kunarti; Agus Subiwahjudi
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.595 KB) | DOI: 10.20473/cdj.v7i1.2017.32-36

Abstract

Background: Streptococcus mutans is a bacteria which has glucosyltransferase (GTF) enzyme and acts as the main agent that causes dental caries. GTF enzyme will convert sucrose into fructose and glucan. Temulawak (Curcuma xanthorrhiza Roxb.) is one of the traditional herbs which has xanthorrhizol, curcumin, flavonoid, tanin, and saponin as an antibacterial agent. Purpose: The purpose of this research is to investigate the effect of temulawak extract (Curcuma xanthorrhiza Roxb.) to the activity of GTF enzyme Streptococcus mutans. Method: This research used 25%, 37,5%, and 50% concentration of  temulawak extract as the treatment, and 0.12% chlorhexidine gluconate as a control. The method of this research consists of three steps; preparing the temulawak extract concentration of 25%, 37,5%, and 50%, preparing the GTF enzyme from the supernatant of Streptococcus mutans, and testing GTF enzyme activity by analyzing the fructose concentration using High Performance Liquid Chromatography (HPLC). Perusal of the fructose area was based on the retention time of fructose. One unit of GTF enzyme activity is defined as the 1 μmol fructose / ml of enzyme / hour. Result: The obtained data then were analyzed by Post-Hoc Tukey (HSD). The result showed a significant difference between each treatment group with the control group (p<0.05). Conclussion: This research concludes that temulawak extract with 25%, 37,5%, and 50% concentrationcan’t inhibit the GTF enzyme activity of Streptococcus mutans.
Perbedaan Daya Antibakteri Antara Kombinasi Calcium Hydroxide-Based Sealer-Amoxicillin Dan Resin-Based Sealer-Amoxicillin Terhadap Bakteri Enterococcus Faecalis Asarizka Bena; Agus Subiwahjudi; Setyabudi Setyabudi
Conservative Dentistry Journal Vol. 8 No. 1 (2018): January - June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (478.267 KB) | DOI: 10.20473/cdj.v8i1.2018.1-4

Abstract

Background. About 24-77% of root canal treatment failure cases are caused by infection of Enterococcus faecalis due to the resistance factor and virulence of these bacteria. Calcium hydroxide-based sealer and resin-based sealer are two types of sealer often used for root canal obturation due to their antibacterial properties. But the antibacterial properties owned by calcium hydroxide-based sealer has no effect on Enterococcus faecalis. Therefore, the adjunct of local antimicrobial such as amoxicillin is needed to increase antibacterial activities of root canal obturation materials (sealers) especially for calcium hydroxide-based sealer. Purpose. The aim of this study was to compare the effectiveness of antibacterial activity of calcium hydroxide-based sealer-amoxicillin and resin-based sealer-amoxicillin against Enterococcus faecalis. Methods. This study is an experimental laboratory with post test only control group design using Enterococcus faecalis ATCC 29212. Agar diffusion test was used to check the antimicrobial activity of calcium hydroxide-based sealer-amoxicillin and resin-based sealer-amoxicillin. by measuring the inhibition zone diameter of each treatment. Results. Resin-based sealer-amoxicillin’s diameter of zone inhibition is 37,3 mm and calcium hydroxide-based sealer-amoxicillin’s diameter is 18,7 mm. Conclusion. Antibacterial activity of resin-based sealer-amoxicillin is significantly greater than calcium hydroxide-based sealer-amoxicillin.
Perbedaan Kekerasan Permukaan Resin Komposit Nanohybrid Setelah Direndam Minuman Soda Bergula dan Beraspartam Rahayu Sukma Dewi; Laksmiari Setyowati; Agus Subiwahjudi
Conservative Dentistry Journal Vol. 8 No. 1 (2018): January - June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.911 KB) | DOI: 10.20473/cdj.v8i1.2018.25-29

Abstract

Background: Nanohybrid composite has filler with variation of size and contain nano-sized particles. The decreasing of surface hardness can be occured because consumption of acid pH beverages, carbondioxide (CO2) from soft drinks and water. Sugary soft drink (coca-cola) has lower pH than aspartame soft drink (coca-cola zero). Besides the difference of pH, aspartame soft drink (coca-cola zero) contains fenilalanin amino acid the product of hydrolisis reaction of aspartame so the acid environment become neutral. Purpose: To view the differences of nanohybrid resin composite after being soaked in sugary soft drink and aspartame soft drink. Methods: There are significant differences between samples being soaked in aquades and aspartame soft drink (coca-cola zero) and sugary soft drink (coca-cola). There are significant differences between aspartame soft drink (coca-cola zero) and sugary soft drink (coca-cola). Conclusion: Surface hardness of nanohybrid resin composite is lower after being soaked in sugary soft drink (coca-cola) than after being soaked in aspartame soft drink (coca-cola zero).
Sitotoksisitas Ekstrak Kulit Kakao (Theobroma cacao) terhadap Kultur Sel Fibroblas BHK-21 Fajariana Fitriani; Adioro Soetojo; Agus Subiwahjudi; tamara yuanita
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (621.928 KB) | DOI: 10.20473/cdj.v9i1.2019.54-65

Abstract

Latar Belakang: Irigasi saluran akar merupakan salah satu tahapan penting untuk menunjang keberhasilan perawatan. Sodium hipoklorit (NaOCl) merupakan larutan irigasi utama yang sering digunakan namun memiliki sejumlah kekurangan yakni bersifat toksik jika diirigasi sampai ke jaringan periradikular. Kakao (Theobroma cacao) merupakan tumbuhan suku Sterculiaeae yang kulit buahnya mengandung senyawa alkaloid, flavonoid, tanin, saponin dan terpenoid. Senyawa-senyawa tersebut terbukti dapat menghambat pembentukan biofilm pada bakteri Enterococcus faecalis yang merupakan bakteri paling resisten pada saluran akar. Ekstrak kulit kakao diharapkan dapat menjadi bahan alternatif irigasi saluran akar yang ideal, sehingga perlu dilakukan penelitian mengenai efek sitotoksisitasnya terhadap jaringan. Tujuan: Menentukan konsentrasi dari ekstrak kulit kakao yang memberikan efek sitotoksik pada sel fibroblas BHK-21. Metode: Jenis penelitian yang dilakukan adalah eksperimental laboratoris dengan menggunakan kultur sel fibroblas BHK-21. Ekstrak kulit kakao diperoleh melalui maserasi menggunakan etanol 70% dan dilakukan pengenceran dengan konsentrasi 1,56%, 3,125%, 6,125%, 12,5%, 25%, 50%, dan 100%. Sel BHK-21 dalam microplate 96 well dipaparkan dengan ekstrak kulit kakao. Uji sitotoksisitas menggunakan MTT assay dan absorbansi warna dibaca menggunakan Elisa reader. Nilai absorbansi dihitung dengan rumus sehingga didapatkan hasil akhir berupa persentase kematian sel. Hasil: Peningkatan konsentrasi ekstrak kulit kakao berbanding lurus dengan kenaikan persentase sel fibroblas BHK-21 yang mati. Kesimpulan: Konsentrasi minimum ekstrak kulit kakao yang dapat memberikan efek sitotoksik pada sel fibroblas BHK-21 adalah 6,25%.
Co-Authors A. Amanda Adioro Soetojo Asarizka Bena Fajariana Fitriani Laksmiari Setyowati Rahayu Sukma Dewi Setyabudi Setyabudi Sri Kunarti Tamara Yuanita