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All Journal Jurnal Akuakultur Indonesia Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Indonesian Aquaculture Journal
Pustika Ratnawati
Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Kampus IPB Darmaga Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Genetic Diversity Analysis of Cultivated Kappaphycus in Indonesian Seaweed Farms using COI Gene Ratnawati, Pustika; Simatupang, Nova F; Pong-Masak, Petrus R; Paul, Nicholas A; Zuccarello, Giuseppe C
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 15, No 2 (2020): August 2020
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v15i2.466

Abstract

Indonesia is a major player in the aquaculture of red algae, especially carrageenan producing ‘eucheumatoids’ such as Kappaphycus and Eucheuma. However, many current trade names do not reflect the evolutionary species and updated taxonomy, this is especially the case for eucheumatoid seaweeds that are highly variable in morphology and pigmentation. Genetic variation is also not known for the cultivated eucheumatoids in Indonesia. Therefore, this study aimed to determine the species and the level of genetic variation within species of cultivated eucheumatoids from various farms across Indonesia, spanning 150-1500 km, using the DNA barcoding method. Samples of seaweed were randomly collected at 14 farmed locations between April 2017 and May 2018. For this study the 5-prime end (~ 600 bp) of the mitochondrial-encoded cytochrome oxidase subunit one (COI) was amplified and sequenced. Morphological examination showed that the samples were quite variable in branching pattern and color. All samples collected from farms with floating line cultivation were identified based on COI sequences as Kappaphycus alvarezii and showed no variation in the COI gene. One farm sample with bottom-line cultivation was identified as K. striatus. The low genetic variation is in contrast to the phenotypic variation of samples, indicating that variation and phenotypic responses to environments is still found in samples with implications for growth rates and carrageenan yield and quality. Information about the genetic variation in stocks is important base knowledge for maintaining, expanding and continuing seaweed aquaculture.
Growth and survival of giant gourami juvenile immersed in freshwater containing growth hormone Ratnawati, Pustika; Alimuddin, .; Arfah, Harton; Sudrajat, Agus Oman
Jurnal Akuakultur Indonesia Vol. 11 No. 2 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (892.808 KB) | DOI: 10.19027/jai.11.162-167

Abstract

Growth of giant gourami is relatively slow. Administration of recombinant growth hormone (rGH) by severaltimes immersion in water containing 0.9% NaCl for one hour can significantly increase the growth. In thisstudy, giant gourami juvenile were immersed in freshwater containing 120 mg/L rGH to determine the longimmersion time that provides higher growth and survival. Juvenile at seventh-day-old after egg yolk absorbedwas immersed for 0.5, 1.0, 2.0, and 3.0 hours in rGH solution. As controls, fish was immersed in freshwaterwithout rGH (control 1: C1), and water containing 0.9% NaCl, and 120 mg/L rGH (control 2: C2). Fish waskept under controlled conditions in 20 L aquarium for three weeks, then in 50 L aquarium until at end ofexperiment, and fed at satiation. The results showed that highest growth in body weight, survival, and biomass(p<0.05) were obtained in rGH immersion treatment for 0.5 hour. Biomass of fish immersed by rGH for 0.5hour was about 32% higher compared to control C1, and 13% compared to C2. Survival of the 0.5 hour rGHimmersedfish (100%) was similar to control C1 and C2. Thus, immersion of giant gourami juvenile infreshwater containing rGH for 0.5 hour allowed highest growth rate, and this method is relatively easy to beadopted by fish hatchery.Keywords: immersion, hormone, growth, freshwater, giant gourami
GENETIC DIVERSITY, GROWTH AND CARRAGEENAN QUALITY OF KOTONI (RED SEAWEED) ACROSS THREE CULTIVATION SITES IN EASTERN INDONESIA Fadilah, Siti; Pong-Masak, Petrus Rani; Farman, Aditia; Ratnawati, Pustika
Indonesian Aquaculture Journal Vol 19, No 2 (2024): (December, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.2.2024.167-178

Abstract

Indonesia is a leading global producer of seaweed, with kotoni seaweed highly valued for its high-quality carrageenan, an essential ingredient in various industries. This study aimed to evaluate the growth, carrageenan quality, and genetic diversity of kotoni seaweed cultivated at three distinct sites in eastern Indonesia: Banggai, West Halmahera, and Biak. The research involved cultivating kotoni seaweed over a 45-day period using the long-line technique, with growth monitoring conducted every five days and water quality parameters measured concurrently. Additionally, ex situ analyses of nitrate, phosphate, and ammonia were performed every 15 days. Carrageenan quality was assessed by measuring carrageenan content, gel strength, and viscosity, in addition to proximate composition analysis. Genetic diversity was evaluated using the cytochrome oxidase subunit 1 (COI) gene, involving DNA extraction, PCR amplification, and sequencing to determine genetic similarity across the cultivation sites. Significant differences in growth rates and carrageenan quality were observed across the three sites. West Halmahera exhibited the highest growth rate, making it the most favorable site for large-scale seaweed cultivation. Although Biak had a lower growth rate, it produced carrageenan with superior gel strength and viscosity, indicating higher product quality. Genetic analysis confirmed 100% similarity among the samples across sites. These findings underscore the importance of site-specific cultivation practices to optimize both yield and carrageenan quality, supporting the sustainability and economic viability of kotoni seaweed cultivation in Indonesia.
Co-Authors Agus Oman Sudrajat Alimuddin Farman, Aditia Harton Arfah Paul, Nicholas A Petrus Rani Pong-Masak Pong-Masak, Petrus R Simatupang, Nova F siti fadilah Zuccarello, Giuseppe C