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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Pengolahan Hasil Perikanan Indonesia agriTECH EnviroScienteae JMM (Jurnal Masyarakat Mandiri) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Grouper: Jurnal Ilmiah Perikanan Fish Scientiae : Jurnal Ilmu-Ilmu Perikanan dan Kelautan

Yuspihana Fitrial

Universitas Lambung Mangkurat

Author-ID : 3593552

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Public Health Social Sciences Veterinary Other

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AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT BIJI TERATAI (Nymphaea pubescens Willd) AKIBAT PEMANASAN Yuspihana Fitrial
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 14 No 1 (2011): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

The purposes of this study were to evaluate the in uence of heating temperature, 100 ˚C and 121 ˚C (the autoclave temperature) on antibacterial activity and stability of water lily seed ethyl acetate extract. Research was initiated with water lily seed extract using multistage maceration extraction method based on solvent polarity level. Extraction stages beginning with a solvent hexane (non polar), then with ethyl acetate (semi-polar) so that theobtained ethyl acetate extract. In ethyl acetate extracts were subjected to heating at 100 ˚C and 121 ˚C. Each extract that has been treated, tested for antibacterial activity with tested bacteria E. coli and S. Typhimurium by using agar well diffusion method. Observations on the stability of fractions contained in the ethyl acetate extract from the treatment given, performed by using TLC (Thin Layer Chromatographi) with a mobile phase of hexane and ethyl acetate (7:3). The activities of each fraction were tested quantitatively by agar well diffusion method. Based on the results of these studies showed that heating affect the antibacterial activity of ethyl acetate extracts. The higher the temperature, decreased antibacterial activity, however at a temperature of 121 ˚ C, ethyl acetate extracts still have antibacterial activity. The process of heating effect on the stability of the ethyl acetate extract fractions. The fractions that too act as an antibacterial were 5 and 6 that relatively more polar than the fraction of 7,8,9,10 and 11. The both fractions was not stable against heating temperature of 100 ˚C and 121 ˚C.Key words: antibacterial, ethyl acetate extract, fraction, temperature

AKTIVITAS BIOLOGIS TEPUNG BIJI TERATAI PRA-MASAK SEBAGAI PRODUK PANGAN PENCEGAH DIARE Yuspihana Fitrial; Rita Khairina; Ika K Oktaviyanti
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 15 No 2 (2012): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Raw waterlilly seeds are known to have activity to prevent diarrhea. It has been proven through testing both in vitro and in vivo against E. coli K1.1 Enterophatogenic (EPEC), the bacteria that causes diarrhea. This study aimed to determine the biological activity of pre-cooked waterlilly seed flour as the prevention of diarrhea through in vivo testing. Results showed that mice fed a ransum substituted with waterlilly seed flour and intervented with EPEC had an increased in body weight 25% lower compared with controls, as well as a declining in fecal water content average of 2% a day after the EPEC intervention was stopped. Rationing followed by EPEC intervention did not increase the number of microbes, causing reduction in the number of E. coli, and did not cause a decrease in lactic acid bacteria (LAB). Rationing waterlilly seed flour shown to inhibit inflammation in the intestinal villi, preventing necrosis of the intestinal mucosa cell (jejenum), reduce inflammation, and prevent adhesion of E. coli in the intestinal mucosa.Key words: diarrhea, EPEC, jejunum, waterlily seeds pre-cooked flour

Antibacterial Activity of Melanin from Cuttlefish and Squid Ink Yuspihana Fitrial; Iin Khusnul Khotimah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20 No 2 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Marine environment comprises of many organism which are known to posses bioactive compound as a common means of self-defense or for the protection of eggs and embryos. Class Cephalopods (such as squidand cuttlefish) are notable for their defences, such as jetting escape movements, changes in colouration, toxic venom and inking.This study aims to compare the antibacterial activity of melanin from cuttlefish ink (Sepia sp.) with squid ink (Loligo sp.) against E. coli. Extraction and purification studies were carried out on Sepia and Loligo melanin using a hydrochloric acid 0,5M treatment under mechanical.The melanins were obtained and further evaluated their activity by direct contact methods between melanin and E. coli in nutrient broth.Total microbes was counted by total plate count.Both inks also was tested their activity against E. coli. The results showed that melanin from cuttlefish and squid inks had inhibitory activity at concentrations of 10 mg / ml and 20 mg / mL, respectively reaching 99.99% against E. coli.The inks of both Cephalopods at the same concentration as melanin, did not show any inhibitory activity against E. coli. The melanin of Sepia sp. have a higher antibacterial activity than the melanin of Loligo sp.

Co-Authors Agung Nugroho Agustiana Fatmawati Fatmawati Herni Noormaliani I Komang Gede Wiryawan Iin Khusnul Khotimah Iin Khusnul Khotimah Iin Khusnul Khotimah Ika K Oktaviyanti Komang G Wiryawan MADE ASTAWAN Nooryantini Soetikno Nooryantini Soetikno Nooryantini Soetikno Nooryantini Sutikno Raudatul Dahliana Safitri Rita Khairina Rizqina Ananda Putri Soewarno S Soekarto Soewarno Soekarto Tutik Wresdiyati Yasmin Finivera Putri

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