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Aeromonas hydrophila Bivalen Vaccines to Improve Body Resistance catfish (Pangasius hypophthalmus) Against Attacks Motile Aeromonas Septicemia Dwi Mailani; Fatmawati Fatmawati; Noor Arida Fauzana; Olga Olga
Jurnal Perikanan dan Kelautan Vol 10, No 1 (2020)
Publisher : JURNAL PERIKANAN DAN KELAUTAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jpk.v10i1.8286

Abstract

Motile aeromonas septicemia/MAS diseases due to Aeromonas hydrophila infection is an obstacle in cultivation of catfish (Pangasius hypophthalmus). MAS control can be done by administering vaccines. The purpose of this research is to get the right and effective dose of bivalent vaccine for controlling MAS at catfish. Both isolates will be used as vaccines are local strains of A. hydrophila CASO.01.G and SBMI.2. Both of them were isolated from disease catfish in the aquaculture ponds in Cindai Alus and Sungai Batang villages, Banjar Regency, South Kalimantan. Vaccines are made by heating (heat killed) at 100oC for 60 minutes. A total of  13 test catfish measuring 9-13 cm were included in each aquarium. The design used was a completely randomized design with 5 treatments and 3 replications, namely bivalent vaccine with a dose of 109 cells/ml, 108 cells/ml, 107 cells/ml, 106 cells/ml, and control (PBS solution pH 7.0). The highest antibody titers in treatments vaccinated fish at a dose of 107 cells / ml (1,706.67). The effectiveness of the vaccine can be seen by the high RPS value of the treatment dose 109 cells/ml (97.22%); 108 cells/ml (100%); 107 cells/ml (97.22%); and 106 cells/ml (100%). The control MTD value was 1 day with 97.43% mortality, while in the vaccine treatment with dose of 107 cells/ml, there was a MTD repeat value for 1 day, but only 1 fish died. Water quality of this research are supportive and not a cause of fish death during the challenge test
Pemberian Arang Aktif Dari Cangkang Kelapa Sawit Terhadap Penyerapan Logam Berat Kadmium (Cd) Dan Tembaga (Cu) Pada Air Asam Tambang Irwansyah Noor; Bambang Joko Priatmadi; Fatmawati Fatmawati; Kissinger Kissinger
EnviroScienteae Vol 16, No 2 (2020): EnviroScienteae Volume 16 Nomor 2, Agustus 2020
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/es.v16i2.9653

Abstract

One of the solutions for solving the acid mine drainage problem of heavy metal like cadmium (Cd) and cooper (Cu) is by using activated charcoal from palm oil shell and activating H3PO4 to enlarge pores of charcoal surface, then it can adsorp maximum amount of heavy metal. The purposes of this research are for analyzing the characteristic of activated charcoal from palm oil shell by activating H3PO4, analyzing the effect of applicated charcoal with or without activating H3PO4 for heavy metal like Cd, Cu and pH in acid mine drainage, and analyzing the variations of the most effective dose of heavy metal adsorption like Cd, Cu and pH enhancement. This research using the experiment method for testing the ability of activated charcoal from palm oil shell for cadmium (Cd) and cooper (Cu) heavy metal adsorption in acid mine drainage using Completely Randomized Design Non Factorial using one factor of treatment and difference of activated charcoal doses are 5 g, 10 g, 20 g dan 30 g with three times of repetitions. The result of this research showing that the characteristic of charcoal from palm oil shell can be used as an ingredient for making the activated charcoal that fulfill the quality of activated charcoal accordance with the standart of SNI 06-3730-95, the application of activated charcoal from palm oil shell by activating H3PO4 which iseffected for Cd and Cu heavy metal and pH of acid mine drainage, the application of activated charcoal from palm oil shell with or without activating H3PO4 is effected for pH of acid mine drainage with a little difference that activated charcoal has better result than the unactivated charcoal, and the most effective dose of variations of activated charcoal by using H3PO4 is 5 g for adsorpting Cd and Cu and the most effective dose for enhancing pH is 30 g.
PEMODELAN DINAMIK KUALITAS AIR PADA BUDIDAYA IKAN NILA (Oreochromis niloticus ) DI PERAIRAN RAWA Fatmawati Fatmawati; Noor Arida Fauzana
EnviroScienteae Vol 10, No 3 (2014): EnviroScienteae Volume 10 Nomor 3, November 2014
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/es.v10i3.1975

Abstract

Increased  feeding at the waste of  feed and feces are discharged into the aquatic environment  make increase the consumption of dissolved oxygen  to decompose. If it continues, there will be an aerobic and reductive conditions, especially in bottom water, they  will be accelerate the decomposition of organic material in aerobic to produce toxic materials which endanger the fish. Purpose of this study was simulate the dynamic model of water quality in tilapia farming in the swamp waters, so can make a good scenario in produced tilapia farming activities in production of appropriate sustainable land productivity capabilities. Data analysis of dynamic model was performed with Power Sim Studio 5. simulation results showed  the growth of tilapia with probiotics feed better than without probiotic feed, and environmental improvements swamp waters. Recommended on first scenario (one crop) and second scenario (two crops) in a year , there is a time break of reproduction  if  harvest done 1-2 times a year for recovery (self purification) natural wetlands, then can create more sustainable aquaculture. The third simulation (three harvests a year) are not recommended, because the land  used continuously without a break will cause impaired water quality and impact on the decline in fish production as well as unsustainable.
PEMANFAATAN RUMPUT AIR (Hydrilla verticillata) SEBAGAI KOMPOS PADA TANAMAN BUNGA KOL (Brassica oleracea) Raudatul Dahliana Safitri; Agung Nugroho; Yuspihana Fitrial; Fatmawati Fatmawati
EnviroScienteae Vol 15, No 2 (2019): EnviroScienteae Volume 15 Nomor 2, Agustus 2019
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.258 KB) | DOI: 10.20527/es.v15i2.6971

Abstract

The purpose of this study was to obtain quality and standards for water grass (H. verticillata) as an alternative raw material for the production of compost fertilizer on cauliflower plants (Brassica oleracea var botrytis L.). The method used in this study used a completely randomized design (CRD), a single factor with analysis of data using ANOVA with a Real Difference (BNT) test. At Po (control) 100% land, P1 100% water grass, 50% P2 grass water + 50% cow manure and 25% P3 water grass + 75% cow manure. The optimal research results on the growth of cauliflower with a ratio of 50% water grass + 50% cow manure with a flower mass parameter of 396g ± 31, plant mass 563 ± 37, root mass 45.31 ± 2 and% NPK content, N 0.99 %, P 0.53%, K 0.32%.
STATUS MUTU AIR SUNGAI SIRAU SUB DAS SIRAU DI DAS BARITO KABUPATEN BARITO TIMUR PROVINSI KALIMANTAN TENGAH Ringkas Lelunuto; Muhammad Ruslan; Kissinger Kissinger; Fatmawati Fatmawati
EnviroScienteae Vol 15, No 2 (2019): EnviroScienteae Volume 15 Nomor 2, Agustus 2019
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.659 KB) | DOI: 10.20527/es.v15i2.6973

Abstract

 Sirau River is one of the rivers in the Barito Timur Regency which flows through the Dusun Timur District. Sirau River water is used as raw material for regional water companies (PDAM) and is used by communities around the river to meet the needs of other daily life such as MCK and other uses and is also used as a landfill, domestic wastewater and waste water mining and plantation activities both directly and indirectly. This study aims to analyze the status of pollution of the Sirau river through the approach of the pollution index value, assess the quality of the Sirau River water and identify the source of pollutants entering the Sirau River. The results showed that in general the water quality of the Sirau River for pH, COD, BOD, Fe, and Mn parameters exceeds the water quality standard namely Government Regulation No. 82 of 2001 class II concerning Water Quality Management and Water Pollution Control, the main source of pollutants comes from residential domestic waste, coal mining activities and dry leaves that fall into river streams. Sirau river water quality from upstream to downstream based on river water quality analysis using the pollution index method shows that water quality has decreased where the river conditions are generally mildly polluted. The quality of lightly polluted Sirau river water cannot be utilized in accordance with the designation of class II water based on Government Regulation No. 82 of 2001 concerning Management of Water Quality and Water Pollution Control.
Estimasi Daya Dukung Mass Balance terhadap Efluen Tambak di Desa Sebamban Baru Kabupaten Tanah Bumbu Provinsi Kalimantan Selatan Fatmawati Fatmawati; K Soewardi; T. Kusumastanto; L. Adrianto
Fish Scientiae Vol 1 No 1 (2011): Issue June-Fish Scientiae Journal
Publisher : Faculty of Fisheries and Marine Resources of Lambung Mangkurat University-South Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (67.026 KB)

Abstract

The purpose of this study was to estimate the carrying capacity of coastal waters ofthe pond effluent. The data required for analysis of mass balance models of carryingcapacity is based on the total ammonia-nitrogen (NH3-N) concentration in coastalwaters. Estimation of carrying capacity with the concept mass balance model usingthe formula Tchobanoglous (1990) and Predalumpaburt (1996) in Tookwinas (1998)which has been modified by including the formula Widigdo and Pariwono (2001). Carrying capacity of coastal waters of Sebamban Baru Village, Tanah BumbuRegency maximum pond area calculation may be developed for aquaculture area of 412,583 hectares, this shows that the concentration of ammonia released by thepond has not been an impact on the quality of coastal waters because the existingpond area is new opened an area of 368.542 ha has not exceeded the carryingcapacity of the mass balance.
PEMETAAN PERUBAHAN PEMANFAATAN LAHAN MANGROVE MENJADI LAHAN BUDIDAYA TAMBAK STUDI KASUS TAMBAK DESA SEBAMBAN BARU KABUPATEN TANAH BUMBU PROVINSI KALIMANTAN SELATAN Fatmawati Fatmawati; Baharuddin Baharuddin
Fish Scientiae Vol 2 No 1 (2012): Issue June-Fish Scientiae Journal
Publisher : Faculty of Fisheries and Marine Resources of Lambung Mangkurat University-South Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (540.307 KB)

Abstract

The purpose of this study was to determine the change in mangrove use to aquaculture. The research was conducted in the village of Sebamban Baru, Sungai Loban District, Tanah Bumbu Regency of South Kalimantan Province. processing of Landsat ETM imagery for mapping mangrove and ponds land use change from 2001and 2010 using ER Mapper software version 7.0.The results showed that the change of mangrove land in the village of Sebamban Baru from 2001 to 2010. 2001 based on the interpretation of the image of vast mangrove 482,321 ha and 254,731 ha of ponds wide, whereas in 2010 there isa change widespread utilization of mangroves into fishponds 346,814 ha and368,542 ha. cause land area of 135 507 ha of mangrove decline and an increase inponds 135,507 hectares wide. From the ecological aspect is emphasized that the formation of spatial coastal areas in Tanah Bumbu district must consider the mangrove forests, and keep doing aquaculture activities and still maintain mangrove conservation by setting the ratio of mangrove land available if the location was built fishpond.
EFIKASI RUTE VAKSIN Aeromonas hydrophila ASB-01 PADA IKAN GABUS (Ophiocephalus striatus) Olga Olga; Fatmawati Fatmawati
Fish Scientiae Vol 3 No 2 (2013): Issue December-Fish Scientiae Journal
Publisher : Faculty of Fisheries and Marine Resources of Lambung Mangkurat University-South Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.8 KB)

Abstract

This study aims to determine the vaccine A.hydrophila ASB-01 is effective forthe control of MAS on snakehead fish. Effectiveness of vaccination was evaluated through the antibody titer, survival, RPS (relative percent survival) and RWK (meantime of death). The study consisted of 5 treatments (immersion vaccination (R), oral(O), intramuscular injection (IM), intraperitoneal injection (IP) and control (PBS pH7.0) with 3 replications. Vaccination doses were 107 cells / ml. Booster vaccination after 14 days later. Dose is equal to the initial vaccination. Furthermore, the next day14 fish were challenged with A.hydrophila ASB-01. To obtain data on antibody titerblood draw done at the time before being vaccinated, shortly before the booster vaccination and 14 days after the booster vaccination. Challenged fish were observedfor 14 days to obtain data on survival, RPS and RWK. The result showed that allthese vaccines may increase the antibody titer, but the highest antibody titers obtained from fish vaccinated injection. Survival rates were vaccinated IM (84.47%), IP(82.20%), R (42.27%), O (42.20%) and controls (13.13%). RPS to IM (82.08%), IP(79.46%), R (33.38%), O (33.31%), while RWK through the IP (3.63 days), IM(79.46 3.57 days), R (2.46 days), O (1.85 days) and controls (1.03 days). Effective vaccination route is through injection.
SUBSTITUSI TEPUNG IKAN DENGAN TEPUNG GONDANG (Pila ampulacea) DAN TEPUNG KALAKAI (Stenochlaena palustris (Burm.) bedd) PADA PAKAN IKAN GABUS HARUAN YANG DIPELIHARA DI AKUARIUM Fatmawati Fatmawati; Noor Arida Fauzana; Pahmi Ansyari
Fish Scientiae Vol 8 No 2 (2018): Issue December-Fish Scientiae Journal
Publisher : Faculty of Fisheries and Marine Resources of Lambung Mangkurat University-South Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.648 KB)

Abstract

Tujuan dari penelitian ini adalah untuk menganalisis substitusi tepung ikan dengan tepung gondang dan tepung kalakai terhadap pertumbuhan, rasio konversi pakan dan tingkat kelansungan hidup ikan gabus haruan (Channa striata). Penelitian ini menggunakan Rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 ulangan.Perlakuan A (pellet tanpa substitusi/Kontrol), B :pellet dengan perbandingan tepung ikan, tepung gondang dan tepung kalakai (50%: 25%: 25%), C :Pemberian pakan pellet dengan perbandingan tepung ikan, tepung gondang dan tepung kalakai (25%: 32,5%: 32,5%), D :Pemberian pakan pellet dengan perbandingan tepung ikan, tepung gondang dan tepung kalakai (0%: 50%: 50%). Parameter yang diamati adalah analisis proksimat pakan, pertumbuhan relatif berat, pertumbuhan relatif panjang, dan kualitas air.Hasil penelitian menunjukkan bahwa hasil uji proksimat terbaik pada komposisi protein perlakuan A (kontrol) 43,5% sedangkan perlakuan dengan subsitusi tepung gondang dan tepung kalakai pada perlakuan B,C dan D berkisar antara 36.22-37,99%. Substitusi tepung gondang dan tepung kalakai mempengaruhi pertumbuhan relatif panjang, tidak berbeda nyata antar perlakuan, pertumbuhan panjang tertinggi pada perlakuan D, sedangkan berat relatif tidak berbeda nyata antar perlakuan, berat relatif tertinggi pada perlakuan D. Kualitas air: suhu, Oksigen terlarut, pH dan amoniak mendukung kehidupan ikan gabus haruan yang dipelihara.
VIRULENSI BAKTERI Edwardsiella ictaluri PENYEBAB PENYAKIT ENTERIC SEPTICEMIA OF CATFISH (ESC) PADA IKAN PATIN (Pangasius pangasius) Nurul Huda Noor Azmi; Fatmawati Fatmawati; Olga Olga
Fish Scientiae Vol 11 No 1 (2021): Issue June-Fish Scientiae Journal
Publisher : Faculty of Fisheries and Marine Resources of Lambung Mangkurat University-South Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.998 KB) | DOI: 10.20527/fishscientiae.v11i1.164

Abstract

Penyakit infeksius yang menyerang ikan patin, salah satunya disebabkan bakteri Edwardsiella ictaluri. Bakteri ini menyebabkan penyakit sistemik enteric septicemia of catfish (ESC). Penelitian bertujuan untuk mengetahui gejala klinis dan rerata waktu kematian akibat serangan bakteri E. ictalury pada ikan patin. Bakteri diinokulasi ke media BHIA (Brain Heart Infusion Agar, merek Conda Pronadisa) dan BHIB (Brain Heart Infusion Broth, merek Merck) diinkubasi pada suhu 24 oC selama 24 - 48 jam. Suspensi bakteri dengan kepadatan 2,7 x 101 – 1010 cfu/mL diinfeksikan pada 300 ekor ikan patin berukuran 11-12 cm dan diamati selama 7 hari. Penelitian menggunakan metode eksperimental dan Completely Randomized Design (CRD). Analisis lebih lanjut dengan Duncan multiple range test (DMRT). Hasil Penelitian menunjukkan gejala klinis yang muncul pada ikan patin antara lain, ikan berenang secara vertikal, warna tubuh pucat, nekrosis, petechiae, liver pucat dan membesar, ginjal berwarna gelap dan kaku, asites insang pucat. Nilai rerata waktu kematian (RWK) ikan patin berkisar antara 1,16 – 2,26 hari. Infectious diseases that attack catfish, one of which is caused by the bacterium Edwardsiella ictaluri. This bacterium causes systemic enteric septicemia of catfish (ESC). The aim of the study was to determine the clinical symptoms and the mean time of death due to the attack of E. ictaluri bacteria on catfish. Bacteria were inoculated into BHIA media (Brain Heart Infusion Agar, Conda Pronadisa brand) and BHIB (Brain Heart Infusion Broth, Merck brand) were incubated at 24 oC for 24-48 hours. Bacterial suspension with a density of 2.7 x 101 – 1010 cfu/mL was infected with 300 catfish measuring 11-12 cm and observed for 7 days. The study used experimental methods and completely randomized design (CRD). Further analysis with Duncan multiple range test (DMRT). The results showed clinical symptoms that appeared in catfish, among others, fish swimming vertically, pale body color, necrosis, petechiae, pale and enlarged liver, dark and stiff kidneys, ascites, pale gills. The mean time of death (MTD) of catfish ranged from 1.16 to 2.26 days.