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Antibacterial Activity of Water lily Seed Extract Toward Diarrhea-causing Pathogenic Bacteria Yuspihana Fitrial; Made Astawan; Soewarno S Soekarto; Komang G Wiryawan; Tutik Wresdiyati; Rita Khairina
Jurnal Teknologi dan Industri Pangan Vol. 19 No. 2 (2008): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.455 KB)

Abstract

The objectives of this study was to observe antibacterial activities of water lily seed, against diarrhea-causing pathogenic bacteria as well as lactic acid bacteria; phytochemistry components in water lily seed and to evaluate each component’s activities against pathogen bacteria. Extraction of antibacterial components in the seed was done by fractional extraction methods using solvent based on its polar level, i.e. hexane, ethyl acetate and ethanol. The activities of each extract was tested by using diarrhea-causing bacteria, Enteropathogenic Escherichia coli K.1.1 (EPEC K1.1) and S. typhimurium with agar well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were calculated with plate counting. Qualitative phytochemical tests were performed on all extracts. Fractionation was performed on extract with the largest antimicrobial activity by using thin-layer chromatography. The activities of each fraction were tested qualitatively by bio-autography method on thin layer chromatographic plates. The water lily seed had an antibacterial activity against EPEC K.1.1 and Salmonella typhimurium, especially in ethyl acetate extract. Ethanol extract had the same, yet lower activity. Ethyl acetate and ethanol extract of the seed did not show inhibition against the growth of lactic acid bacteria (Lactobacillus sp) and Bifidobacterium bifidum. The MIC and MBC values of the ethyl acetate extract on EPEC K1.1 were 0.89 (mg/mL) and 1.33 (mg/mL), respectively, while similar values of that on S. Typhimurium were 1.11 (mg/mL) and 1.33 (mg/mL¬), respectively. Phytochemistry components within ethyl acetate extract were alkaloids, flavonoids, tannins, glycosides, saponins, and triterpenoids. All fractions in the ethyl acetate extract had antimicrobial activities against EPEC K.1.1 and S. Typhimurium. These fractions were thought to inhibit the growth of the test-microbes by synergic action of each component.Key words: water lily seed, antibacterial, ethyl acetate extract
The effect of passion fruit hulls level (Passiflora edulis Sims f. edulis Deg) as Kacang goat feed component: I. Intake digestibility and nitrogen retention Kiston Simanihuruk; Komang G Wiryawan; Simon P Ginting
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 2 (2006): JUNE 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.742 KB) | DOI: 10.14334/jitv.v11i2.513

Abstract

To study the effect of utilization of passion fruit hulls (Passiflora edulis Sims f. edulis Deg) as feed component on consumption, digestion and nitrogen retention in kacang goats, a trial was conducted using 20 young kacang goats (average initial body weight 23.73±2.16 kg). The experiment was arranged in completely randomized design consisting of 4 diets and 5 replications. Animal were randomly allocated into 4 diets (0, 15, 30, 45% of passion fruit hulls). Each diet contains 2550 Kcal/kg metabolism energy and 14% crude protein. The feeding level was set at 3.8% of body weight based on dry matter. The results of the experiment showed that average dry matter intake, nutrient digestibility, and nitrogen retention were not affected by level of passion fruit hulls (P>0.05), although nutrient digestibility and nitrogen retention tended to decrease with the increasing level of passion fruit hulls. It was concluded that passion fruit hulls can be used till 45% level in the diet. Key Words: Passion Fruit, Consumption, Digestibility, Nitrogen Retention, Kacang Goat
Karakteristik Endapan Cairan Rumen Sapi asal Rumah Potong Hewan sebagai Feed Supplement Agus Budiansyah; Resmi Resmi; Nahrowi Nahrowi; Komang G Wiryawan; Maggy T Suhartono; Yantyati Widyastuti
Jurnal Ilmiah Ilmu-Ilmu Peternakan Vol. 14 No. 1 (2011): Mei 2011
Publisher : Fakultas Peternakan Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.854 KB) | DOI: 10.22437/jiiip.v0i0.829

Abstract

The aims of this experiment were to  identify and characterize of sediment product from cattle rumen liquor as a source of amino acids, minerals and vitamins. The sediment were obtained as pellets upon centrifugation of rumen liquor at 10,000 g for 10 minutes for collection of supernatant. The sediment were evaluated for amino acids, minerals and vitamins composition and chararacterized for the pH,  solubility of dry matter, specific density, bulk and compacted bulk densities and angle of response. Result of the experiment showed that sediment contained higher minerals:  Na, K and Fe compared with the commercial premix, but lower in B-vitamins and amino acids. The composition of mineral Na, K, and Fe from rumen liquor of local cattle was 13.47%, 7.73 % and 14.52 %,  while Na, K, and Fe from  rumen liquor of  imported cattle was 18.40%, 10.25%, and 14.52% respectively. The sediment had pH range from 10.01-10.03, the dry matter solubility  was 35.5% up to 39.1%. The sediment from imported cattle had higher specific density, bulk and compacted bulk densities and angle of response than that of local cattle. It is concluded that sediment from cattle rumen liquor contained high Na, K and Fe, low amino acids and B-vitamin, high pH and low solubility.
Kandungan Lemak, Koesterol dan Profile Asam-asam Lemak Daging Kerbau Yang Disuplementasi Pakan Campuran Garam Karboksilat Kering: Supplementation Effect of Dried Carboxylate Salt Mixture on Fat content , Cholesterol And Profile Fatty Acids of Buffalo Meat Yurleni Yurleni; Rudi Priyantoi; Komang G Wiryawan
Jurnal Ilmiah Ilmu-Ilmu Peternakan Vol. 21 No. 1 (2018): Mei 2018
Publisher : Fakultas Peternakan Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.346 KB) | DOI: 10.22437/jiiip.v21i1.5404

Abstract

Penelitian bertujuan untuk mengkaji pengaruh suplementasi campuran garam karboksilat kering (CGKK) yang berasal dari minyak ikan terhadap kandungan lemak, kolesterol dan profil asam-asam lemak daging kerbau. Daging berasal dari kerbau jantan umur 1,5-2 tahun yang dipelihara secara intensif selama 2,5 bulan dengan pemberian pakan hijauan 35% dan konsentrat 65%. Suplementasi CGKK berbahan dasar minyak ikan lemuru ditambahkan sebanyak 45 g/kg konsentrat. Setelah pemeliharaan ternak dipotong. Untuk analisa daging digunakan otot longissimus dorsi. Penelitian menggunakan rancangan acak lengkap apabila ada perbedaan di uji'dengan uji lanjut Least Square MeansModels (LSMEAN). Hasil penelitian memperlihatkan bahwa suplementasi CGKK menurunkan kandungan asam-asam lemak jenuh daging kerbau dan meningkatkan kandungan asam-asam lemak tak jenuh daging kerbau. Dapat disimpulkan bahwa suplementasi CGKK meningkatkan profil asam-asam lemak tak jenuh terutama asam lemak omega-3 yaitu EPA dan DHA. Kata kunci : kerbau, suplementasi, minyak ikan, asam-asam lemak daging.
KECERNAAN NUTRIEN PAKAN TEPUNG DAUN MURBEI PADA SAPI PERANAKAN ONGOLE Duta Setiawan; Komang G Wiryawan
JURNAL ILMIAH PETERNAKAN TERPADU Vol 3, No 4 (2015)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.164 KB) | DOI: 10.23960/jipt.v3i4.p%p

Abstract

The  research was conducted to study the ability of mulberry leave meal addition concentrates on the digestibility  nutrient  of Ongole Grade. This experiment used a randomized block design, with 4 treatments and 4 blocks. Treatments consisted of P1 (native grass and concentrate feed in the form of mulberry leaves meal and concentrate complete), P2 (native grass and concentrate feed in the form of mulberry leaves meal and corn concentrate), P3 (native grass and concentrate feed in the form of mulberry leaves meal and rice bran concentrate), P4 (native grass and concentrate feed in the form of mulberry leaves meal and concentrate tapioca waste). The experiment was conducted for 4 months with the adaptation periods for 2 weeks. Parameters measured were digestibility of dry matter (KCBK) and organic matter (KCBO), . The results showed that the mulberry leave meal addition on different concentrate did  significantly (P<0,05) on digestibility of dry matter (KCBK) and organic matter (KCBO). It is concluded that based on the nutrient digestibility, mulberry leave meal concentrate can be combined with a single concentrate such as corn, cassava waste meal or complete concentrate. Keywords: mulberry leave meal, ongole grade cattle, digestibility of dry matter  and organic matter.