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Polimorfisme Penanda RAPD untuk Analisis Keragaman Genetik Pinusmerkusii di Hutan PendidikanUnhas Gusmiaty, Gusmiaty; Restu, Muh.; Asrianny, Asrianny; Larekeng, Siti Halimah
Jurnal Natur Indonesia Vol 16, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.822 KB) | DOI: 10.31258/jnat.16.2.47-53

Abstract

The aims of Genetic study of pinus identified stand in Unhas Experimental Forest is to analyses of genetic characteristics of stand, based on RAPD (Random Amplified Polymorphic DNA) marker. The study was conducted in Biotechnology and Tree Breeding Laboratory, Forestry Faculty, Hasanuddin University. The method are DNA isolation, Primers selection and RAPD analyses. DNA analyses of Pine with ten RAPD marker showed number of alel varietied and polymorphic. Coefficient of similarity in population have number of 0.15-0.73.Highest genetic distance is 0.9630 and lowest genetic distance is 0.2698. Number of genetic diversity of Pine in Experimental Forest Hasanuddin University is 0,489 and categorized highly.
PRODUKSI POLONG DAN BIJI TANAMAN GAMAL (Glirisidia sepium) DARI BERBAGAI PROVENANSI DENGAN PEMUPUKAN NPK Muh. Restu; Baharuddin Mappangaja
PERENNIAL Vol. 2 No. 1 (2006)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v2i1.150

Abstract

The productivity of forest natural resources is now decreasing and therefore rehabilitation efforts is urgently needed. Breeding of forest plant is the best solution to increase forest productivity in both quantity and quality. The biological reproduction can be conducted through seed production with fertilization. This research was aimed at determining the effect of fertilizing treatments on pod and seed production of different Glirisidia sepium provenances to find out the best provenance and best fertilizer dosage. Fertilization treatments showed a significant effect on the pod and seed production. On the other hand, the provenance and its interaction with fertilizing did not show any significant effects on the pod and seed production. The best performance was found on the fertilization treatment of 500 g resulting in the average production of 1,288 pods and 27,621 seeds/tree. Keywords: Fertilizing, Seed Production, Provenance, Glirisidia sepium
POTENSI DAN PERMUDAAN TEGAKAN ALAM EBONI (Diospyros celebica Bakh.) DI AREAL HPH PT. INHUTANI I MAMUJU Muh. Restu
PERENNIAL Vol. 2 No. 2 (2006)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v2i2.160

Abstract

The intensive use of Eboni has caused a decrease of its natural population which has also contributed to the future loss of genetic potencies. Preservation efforts could only be conducted if the actual potencies of the stand and its environmental conditions were recognized. A study on stand and regeneration potencies as well as its soil characteristics is needed as a basic in management aplication for the increase of genetic potencies. This study used a systematic sampling survey method and laboratory analysis. The study aims to identify tree potencies and regeneration of Eboni Makassar as well as its soil characteristics under the stand. The results are expected to provide a description on Eboni Makassar potencies and its management efforts. It was found from the study that the potency of Eboni was 5.013 m3/ha with minimum and maximum estimated volumes of 3.163 m3/ha and 6.863 m3/ha, respectively. Eboni was naturally regenerated better in >40% slope category. Keywords: Eboni, Potencies and Regeneration, Various Slope
SELEKSI PRIMER UNTUK ANALISIS KERAGAMAN GENETIK JENIS BITTI (Vitex coffassus) . Gusmiaty; Muh. Restu; Ira Pongtuluran
PERENNIAL Vol. 8 No. 1 (2012)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v8i1.211

Abstract

The successful of genomic DNA amplification using RAPD technique was determined by the sequence of primer’s base and primer compound in each reaction. The aim of research was finding the best primer for genetic diversity analyses of Bitti (Vitex coffassus). The results of amplification showed that number of band between one to five bands (110-600 bp). The primer of OPK-10, OPA-17, OPQ-07 and OPP-08 can be used for genetic diversity analyses of Bitti. The best primer used for genetic diversity analyses of Bitti was OPP-08 because it had the highest number of bands (5 bands). Key words: OPP-08, Primer Selection, Vitex coffassus
PENGARUH DOSIS INOKULAN ALAMI (EKTOMIKORIZA) TERHADAP PERTUMBUHAN SEMAI TENGKAWANG (Shorea pinanga) . Gusmiaty; Muh. Restu; Ayu Lestari
PERENNIAL Vol. 8 No. 2 (2012)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v8i2.217

Abstract

This research aimed to know the effect of some doses of natural inoculants on the growth of Shorea pinanga seedling. The research was carried out with several steps including seed provision, inoculation of ectomycorrhyza, planting and maintenance in the nursery. The result of the research showed that height and number of branches were different at the significant level of 5 %, while diameter and number of leaves were not significantly different. The natural inoculants dose of 15 g showed the best growth in response to the increment of height, diameter, and number of leaves, while that of 20 g showed the best in the number of branches increment. Key words: Shorea pinanga, ectomycorrhyza, seedling, natural inoculantstor, Local Government Revenue, regional autonomy
Bola Benih Sebagai Kiat Baru dalam Rehabilitasi Hutan dan Lahan Kritis di Kecamatan Tinggimoncong, Sulawesi Selatan Muh. Restu; Syamsuddin Millang; Samuel Paembonan; Budirman Bachtiar; Gusmiaty Gusmiaty
Biocelebes Vol. 6 No. 2 (2012)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Rehabilitate forest and farm critical must special attention and need of the new method that more practical, quick, and cheap. Method of seed ball is its alternative method. with consideration referred, then this research bent on for (1) know seed amount every seed ball that give percentage grows and plants growth sengon the best (2) know size of seed ball diameter the best to percentage grow and plants growth sengon; (3) know appropriate type of farm closing to percentage grow and plants growth sengon; (4) know interaction influence between seed amount every ball, level of seed ball, type of the best farm closing to percentage grow and plants growth sengon at the site. This research is executed start month September 2012 up to final December 2012 in district Tinggimoncong Gowa Regency. this research uses random design complete pattern factorial that consisted of 3 factor that is seed amounts every seed ball as first factor with 4 levels that is 5 seeds, 7 seeds, 9 seeds, and 11 seeds every seed ball, second that is large size factor its diameter of seed ball that consisted of 4 levels that is 2.5 cm, 4 cm, 5.5 cm, and 7 cm, third factor that is farm closing that consisted of 4 levels that is opened (grass), coppice, beams, and cleaned (disc with diameter 20 cm). The three of this treatment factor are combined until got counted 4 x 4 x 4 = 64 treatment units. Every treatment unit consisted of 3 seed balls and repeated 3 times until required 64 x 3 x 3 = 576 seed balls. Variable that perceived is time starts germinate and percentage germinates every week during a month first, plants growth (high and leaf amount) every month after second month for 2 month. Research result indicates that treatment of land cover has an effect on reality to germination percentage, leaf amount, and high plants as for seedball diameter, seed amount and interaction between land cover and seedball diameter have an effect on reality to percentage of germination and leaf amount. Treatment of grass land cover gives influence is done well by germination percentage, leaf amount, and high plants. Seedball diameter 7,0 cm and seed amount 11 seeds give influence is done well by germination percentage. Combination of grass land cover treatment and diameter of seed ball 7,0 cm give best influence. Key words: Formulation, biological control agent, Aspergillus sp., and tablet.
KARAKTERISASI MORFOLOGI SUMBER BENIH TEGAKAN PINUS TERIDENTIFIKASI DI HUTAN PENDIDIKAN UNIVERSITAS HASANUDDIN Muh. Restu; Gusmiaty Gusmiaty
Biocelebes Vol. 9 No. 1 (2015)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

This study aims  to investigate genetic diversity of identified pine stands at Unhas Extention Forest based on morphological markers.  The methods used are observation and measurement of the variables leaves, stems and plant height.  An analysis of data used the NTSYSpc program (Numerical Taxonomy and Multivariate System) version 2.0.The results showed that there are some morphological similarities such as  the shape of the leaves (needles), the shape of the curve of the leaf tip (tapered), the shape of the curve of the leaf base(tapering), the shape of the leaf margins (flat), the color of top surface of the leaf (dark green), the color of lower surface of leaf (dark green), the state of the upper surface of the leaf (smooth), the state of the lower leaf surface (rough), bark texture (grooved), rod shape (square), and the shape of the canopy (cones) while the other variables showed a difference. The genetic diversity of individual spine based on morphological observations is low.   Keywords  :  Morfological, Genetics,  Diversity, Pine
Utilization of Leaf Litter as Growth Media for Suren (Toona sureni Merr.) Rhizosphere Fungi Isolates Gusmiati; Muh. Restu; Siti Halimah Larekeng; Budirman Bachtiar; Muhammad Bima Akzad
Journal of Sylva Indonesiana Vol. 3 No. 01 (2020): Journal of Sylva Indonesiana
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1211.401 KB) | DOI: 10.32734/jsi.v3i01.1986

Abstract

Leaf litter or organic matters can decompossed by microorganisms like fungi.This study aims to determine the ability of growth and the rate of decomposition of Suren (Toona sureni Merr.) rhizosphere fungi isolates on various organic media. The testing process was carried out at the Biotechnology and Tree Breeding Laboratory, Faculty of Forestry, Hasanuddin University, Makassar. The research method consisted of making fungi growing media, rejuvenating isolates and making organic media from teak leaf litter (Tectona grandis), Jabon (Anthocephalus cadamba), and Mahogany (Swietenia macrophylla). The highest growth of mycelium was found in Fusarium sp. The growth of the five best fungi isolates in the formulation of mahogany leaf litter and the highest average decomposition rate in the combination treatment of mahogany leaf litter and Fusarium sp.
EFEKTIVITAS PENGGUNAAN PAKAN KOMBINASI ANTARA MAGGOT BSF DAN PAKAN KOMERSIL TERHADAP PERTUMBUHAN DAN SINTASAN IKAN LELE CLARIAS SP Restu, Muh.; Budi, Sutia; Mulyani, Sri
Journal of Aquaculture and Environment Vol. 7 No. 2 (2025): Journal of Aquaculture and Enviroment Juni 2025
Publisher : Postgraduate Bosowa University Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35965/jae.v7i2.4377

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan pakan kombinasi antara maggot BSF dan pakan komersil terhadap pertumbuhan dan sintasan ikan lele. Penelitian ini menggunakan rancangan acak lengkap (RAL) yang terdiri dari empat perlakuan dan tiga kali ulangan. Perlakuan yang diberikan yaitu maggot BSF dan pakan komersil dengan dosis perlakuan A (75%+25%), perlakuan B (50%+50%), perlakuan C (25%+75%), dan perlakuan D pakan komersil (kontrol). Frekuensi pemberikan pakan dilakukan sebanyak dua kali sehari dengan dosis 5% dari bobot tubuh hewan uji. Parameter uji yang diamati yaitu retensi protein, laju pertumbuhan bobot spesifik, sintasan rasio dan konversi pakan. Sedangkan parameter kualitas air meliputi suhu, pH, dan DO. Hasil penelitian menyimpulkan bahwa komposisi pakan uji dengan dosis maggot BSF 25% dan pakan komersil 75% memberikan pengaruh yang nyata terhadap retensi protein dan laju pertumbuhan bobot spesifik, serta tidak berpengaruh nyata terhadap sintasan dan rasio konversi pakan. Hasil retensi protein sebesar 21,54%, laju pertumbuhan bobot spesifik 2,483%, rasio konversi pakan 1,73%, dan sintasan 82,50%. Sedangkan kualitas air selama penelitian masih berada pada kisaran optimal yaitu suhu 27,2°C, pH 7,3, dan DO 6,7 mg/l. The aim of this research was to determine the effect of using a combination of BSF maggots and commercial feed on the growth and survival of catfish. The research used a completely randomized design (CRD) consisting of four treatments and three replications. The treatments given were BSF maggots and commercial feed with doses of treatment A (75%+25%), treatment B (50%+50%), treatment C (25%+75%), and treatment D commercial feed (control). The frequency of feeding is twice a day with a dose of 5% of the test animal’s body weight. The test parameters observed were protein retention, specific weight growth rate, survival ratio and feed conversion. Meanwhile, air quality parameters include temperature, pH, and DO. The results of the study concluded that the composition of the test feed with a dose of 25% BSF maggots and 75% commercial feed had a significant effect on the protein retention and specific weight growth rate, and had no significant effect on survival and feed conversion ratio. The protein retention results were 21,54%, specific weight growth rate 2,483%, feed conversion 1,73%, and survival ratio 82,50%. Meanwhile, air quality during the research was still in the optimal range, namely temperature 27,2°C, pH 7,3, and DO 6,7 mg/l..