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All Journal Indonesian Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology
Imron Riyadi
Balai Penelitian Bioteknologi Perkebunan Indonesia, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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EX VITRO ROOTING OF OIL PALM (Elaeis guineensis Jacq.) PLANTLETS DERIVED FROM TISSUE CULTURE Sumaryono, Sumaryono; Riyadi, Imron
Indonesian Journal of Agricultural Science Vol 12, No 2 (2011): October 2011
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Plantlets of oil palm (Elaeis guineensis Jacq.) derived from so-matic embryos sometimes do not form well developed-roots. Root formation of unrooted-plantlets can be induced with aux-in during ex vitro acclimatization period to simplify the proce-dure and to reduce seedling production cost. Experiments were conducted using a completely randomized design to determine the effect of different types of auxin, i.e. indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and 1-naphthalene-acetic acid (NAA) at different concentrations, i.e. 0, 2, 4, 8, and 16 mM on root development of oil palm plantlets. The plantlets used were derived from somatic embryos of MK 649 oil palm clone. The basal end of the shoots was dipped in auxin solution for 10 minutes before the shoot was cultured in a small plastic pot containing a mixed growing medium. The cultures were then placed inside a closed transparent plastic tunnel (240 cm x 100 cm x 95 cm) for 12 weeks. The results showed that without auxin treatment only 15% of the shoots formed roots. Dipping in auxin solution increased significantly root frequen-cy to more than 50%. The best root formation was found on the shoots treated with 2 mM NAA by which rooting frequency was 80%. Auxin treatments also increased root quality as indi-cated by more number of primary and secondary roots. IAA, IBA, and NAA treatments at all concentrations tested increased significantly shoot height on average by 42% and shoot diame-ter by 30% compared to control treatment, but did not influ-ence root length. The best treatment for inducing roots of oil palm plantlets ex vitro was by dipping the basal end of the plant-lets in 2 mM NAA solution. The result showed that rooting of oil palm plantlets could be successfully conducted ex vitro that would eliminate sterile rooting stage thus simplify the protocol and reduce seedling production time and cost.
Sequence-Structure Comparative and Network-Based Prediction of Drought Gene Candidate Regulator in Elaeis guineensis Permatasari, Galuh Wening; Putranto, Riza Arief; Mardhika, Larasati Dena; Aksa, Annisa Aulia; Setiawati, Yuli; Minarsih, Hayati; Riyadi, Imron; Ernayunita, Ernayunita
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 3 (2024): September
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.90808

Abstract

Drought poses a significant threat to global food security, particularly impacting crops like oil palm. Selecting genes for genome editing to enhance drought tolerance presents formidable challenges. To ensure that the target gene is chosen correctly and results in the desired character, a pilot study is necessary to determine the target gene for knockout. Two genes drought-related, AtBRL3 and AtOST2, were scrutinized in this context. Aligned with the Elaeis guineensis genome, their neighbouring proteins and gene ontology were analysed to identify potential targets for genome editing. AtBRL3, identified as BRL1 (XP_010913986.1) in E. guineensis, exhibited 58.48% identity and 100% coverage. It interacts with 12 nodes, including BIR1, BRI1, and AT2G20050, crucial for signalling pathways and cellular responses. Molecular function analysis revealed kinase activity. AtOST2 showed high similarity to plasma membrane ATPase/HA1 (XP_010913679.1) in E. guineensis, with 87.46% identity and 100% query cover. It correlated with 14 genes associated with ABA stimulus, stomatal movement, and hormone response. EgBRL1 and EgHA1, resembling AtBRL3 and AtOST2, respectively, emerge as promising targets for developing drought-tolerant oil palm cultivars through gene editing. Nonetheless, further validation through in vitro gRNA target selection and in vivo conversion of OST2/BRL3-containing plasmids in oil palm calluses is indispensable to demonstrate their efficacy in conferring novel drought resistance traits. 
Co-Authors Aksa, Annisa Aulia Ernayunita, Ernayunita Hayati Minarsih Mardhika, Larasati Dena PERMATASARI, Galuh Wening Riza Arief Putranto Sumaryono Sumaryono Yuli Setiawati, Yuli