Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Journal of Dentomaxillofacial Science
Wahyu Hidayat
Department of Oral Medicine, Faculty of Dentistry, Padjadjaran University, Bandung, Indonesia
Dentistry

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Evaluation of antibacterial activity and acute toxicity of pomegranate (punica granatum l.) seed ethanolic extracts in swiss webster mice Riani Setiadhi; Irna Sufiawati; Dewi Zakiawati; Nanan Nur’aeny; Wahyu Hidayat; Dani R. Firman
Journal of Dentomaxillofacial Science Vol. 2 No. 2 (2017): (Available online: 1 August 2017)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v2i2.536

Abstract

Objective: To evaluate the safety of a pomegranate seeds ethanolic extract in Swiss Webster mice.Material and Methods: Pomegranate seed’s ethanolic extracts were evaluated for minimum inhibitory concentration against streptococcus sanguis ATCC 10556, using microdilution method through 96-wells microplate. In acute toxicity test, 12 females and 12 males Swiss Webster mice weighing 20–30 grams were divided into four groups, each consisting of 3 females and 3 males. Three groups were orally given of sample with concentrations 0.2% equivalent to 40 mg/kg, 0.4% equivalent to 80 mg/kg, and 0.8% equivalent to 160 mg/kg, while the control group was given distilled water. Observations were made at 30, 60, and 90 minutes, for 24 hours and 7 days.Results: The MIC against Streptococcus sanguis was on 2000 ppm concentration and until 24 hours observation no lethal effect occurred on the mice, but at the 7 days observation in the group of 0.8% per kilogram body weight, the lethal effect occurred at one mice.Conclusion: The ethanolic extract of pomegranate seeds has antibacterial effect and no acute systemic toxicity, which can be considered safe on the use as oral topical solution since the toxic dose is greater than 160 mg/kg of body weight.
Identification of active component in red pomegranate (punica granatum L.) seeds ethanolic extract Riani Setiadhi; Irna Sufiawati; Wahyu Hidayat
Journal of Dentomaxillofacial Science Vol. 4 No. 2 (2019): (Available online: 1 August 2019)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v4i2.934

Abstract

Objective: Pomegranate (punica granatum L.) have many benefits for health and used as traditional medicine. The pomegranate seeds are rich in antioxidants, namely flavonoids and tannins as well as alkaloid which is an antibacterial.  The aim of this study was to identify the pure isolate/active component of red pomegranate seeds ethanolic extract. Material and Methods: Two-ways TLC of the purified crystals which were obtained from the hexan fraction of ethanolic extract red pomegranate seeds, were used to make sure there was only 1 active compound of red pomegranate seeds. The isolate was identified using dragendorff, Mg and HCl, NaOH, Bunchard, Liebermen, FeCL3 and HCl concentrated reagents.Results: Isolate identification test using dragendorf reagents showed positive result (an orange-coloured).Conclusion: The pure isolate/active component of red pomegranate seeds is alkaloid.
Co-Authors Dani R. Firman Dewi Zakiawati Irna Sufiawati Nanan Nur’aeny Riani Setiadhi