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Dani R. Firman
Department of Oral Biology, Faculty of Dentistry, Padjadjaran University, Bandung, Indonesia
Dentistry

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Evaluation of antibacterial activity and acute toxicity of pomegranate (punica granatum l.) seed ethanolic extracts in swiss webster mice Riani Setiadhi; Irna Sufiawati; Dewi Zakiawati; Nanan Nur’aeny; Wahyu Hidayat; Dani R. Firman
Journal of Dentomaxillofacial Science Vol. 2 No. 2 (2017): (Available online: 1 August 2017)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v2i2.536

Abstract

Objective: To evaluate the safety of a pomegranate seeds ethanolic extract in Swiss Webster mice.Material and Methods: Pomegranate seed’s ethanolic extracts were evaluated for minimum inhibitory concentration against streptococcus sanguis ATCC 10556, using microdilution method through 96-wells microplate. In acute toxicity test, 12 females and 12 males Swiss Webster mice weighing 20–30 grams were divided into four groups, each consisting of 3 females and 3 males. Three groups were orally given of sample with concentrations 0.2% equivalent to 40 mg/kg, 0.4% equivalent to 80 mg/kg, and 0.8% equivalent to 160 mg/kg, while the control group was given distilled water. Observations were made at 30, 60, and 90 minutes, for 24 hours and 7 days.Results: The MIC against Streptococcus sanguis was on 2000 ppm concentration and until 24 hours observation no lethal effect occurred on the mice, but at the 7 days observation in the group of 0.8% per kilogram body weight, the lethal effect occurred at one mice.Conclusion: The ethanolic extract of pomegranate seeds has antibacterial effect and no acute systemic toxicity, which can be considered safe on the use as oral topical solution since the toxic dose is greater than 160 mg/kg of body weight.
Time-kill assay of pomegranate (Punica granatum L) seed ethanolic extract against streptococcus sanguis; the cause of recurrent aphthous stomatitis Riani Setiadhi; Irna Sufiawati; Dewi Zakiawati; Dani R. Firman
Journal of Dentomaxillofacial Science Vol. 3 No. 3 (2018): (Available online: 1 December 2018)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1270.668 KB) | DOI: 10.15562/jdmfs.v3i3.787

Abstract

Objective: To examine the antimicrobial activity of pomegranate seed using a time-kill-assay by measuring the number of bacteria left at a various time points after exposure to the substance.Material and Methods: Pomegranate seeds were extracted by a maceration method using 70% ethanol as the solvent. The extract was evaluated for minimum inhibitory concentration and minimum bactericidal concentration against streptococcus sanguis ATCC 10556, using a microdilution method. The concentration used in time-kill-assay were 2 to 4 times MBC value and 48 hours incubation time as the initial value. Correlation of the concentration and time duration using semi-log graph, i.e. the x-axis as time duration with variation interval of 30, 60 and 90-seconds.  Y-axis is the logarithmic value of the bacterial colonies number.Results: Bacteriostatic effect was observed at 4000 ppm concentration at 30, 60 and 90-seconds time intervals. The bactericidal effect was observed at 8000 ppm of concentration at the 90-second time interval, whereas 30 and 60-seconds remained bacteriostatic.Conclusion: The pomegranate seeds ethanolic extract is considered as antibacterial with a concentration-dependent category.
Co-Authors Dewi Zakiawati Irna Sufiawati Nanan Nur’aeny Riani Setiadhi Wahyu Hidayat