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Pujiana E. Lestari
Department of Biomedical Science, Faculty of Dentistry, Jember University, Jember, Indonesia
Dentistry

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Antibacterial activity of essential oil extracts from Curcuma xanthorrhiza roxb. rhizomes against bacteria causing pulp necrosis Dwi Prijatmoko; Nakhita L. Syafira; Pujiana E. Lestari
Journal of Dentomaxillofacial Science Vol. 3 No. 3 (2018): (Available online: 1 December 2018)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (893.656 KB) | DOI: 10.15562/jdmfs.v3i3.763

Abstract

Objective: To analyze antibacterial activity of essential oil extracted from Curcuma xanthorrhiza Roxb. rhizomes against bacteria causing pulp necrosis, Enterococcus faecalis, and Fusobacterium nucleatum.Material and Methods: The method used in this research was disc-diffusion on MH-A media, based on the standard protocol of National Committee for Clinical Laboratory Standards (NCCLS). This method used 4 replications in each of the study group. The study group consists of 4 different concentration groups (25%, 50%, 75% and 100% ) of Temulawak rhizomes essential oil extracts, ChKM and Cresophene were used as the positive control group and DMSO (10%) and Tween 80 (0.5%) was used as the negative control group.Results: The inhibition zones of 25%, 50%, 75%, and 100% of temulawak rhizomes essential oil extracts against E. faecalis were 8.17 mm, 8.54 mm, 9.53 mm, and 10.17 mm, respectively. Whereas, the inhibition zones of 25%, 50%, 75%, and 100% of temulawak rhizomes essential oil extracts against F. nucleatum were 8.54 mm, 8.95 mm, 9.65 mm and 10.75 mm, respectively. Data analysis using Kruskal-Wallis in both of bacteria showed p=0.00 (p<0.05) and therefore, significant levels of inhibition.Conclusion: Essential oil extracts from C. xanthorrhiza Roxb. rhizomes has medium antibacterial activity against E. faecalis and F. nucleatum.
Steeping from green and black robusta coffee beans increase viability of Peripheral Blood Mononuclear Cells (PBMC) and salivary leukocytes which is induced by streptococcus mutans I-Dewa A.R. Dewanti; Roedy Budirahardjo; Ristya WE. Yani; Pujiana E. Lestari; Dyah Setyorini; Eri H. Jubhari; Sunlip Wibisono
Journal of Dentomaxillofacial Science Vol. 4 No. 3 (2019): (Available online: 1 December 2019)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1502.685 KB) | DOI: 10.15562/jdmfs.v0i0.799

Abstract

Objective: Analyzing viability of PBMC and salivary leukocytes  by steeping green coffee and black coffee seeds from Robusta coffee after induced with S. mutans.Material and Methods: Blood and saliva of healthy people added with histopaque centrifugation and suspended in RPMI 1640. The cells were placed in 24-well microtiter plate, treated according to group. The research groups: control: induced by S. mutans, black coffee (2.5%, 5%, 10%), Green coffee (2.5%, 5%, 10%). All coffee groups, both PBMC and saliva groups were induced by S. mutans. Viability was stainted with Tripan Blue. Cells were counted under an inverted microscope with magnification 400 times from an average of 5 fields of view. The data obtained were ANOVA followed by LSD test.Results. ANOVA and LSD analysis showed significant differences (0.01<p). The higher of concentration of Robusta coffee, the more cells that live. On the other hand, Green coffee increased the viability of PBMC and salivary leucocytes more than black coffee. Suspected, because antioxidant of the green coffee that more higher than black coffee, so it is better able to protect cells.Conclusion. Steeping Green and black coffee increase viability of PBMC and leucocytes which is induced by S. mutans.
Co-Authors Dwi Prijatmoko Dyah Setyorini Eri H. Jubhari I-Dewa A.R. Dewanti Nakhita L. Syafira Ristya Widi Endah Yani Roedy Budirahardjo Sunlip Wibisono