Maria Tanumiharja
Department of Conservative Dentistry, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia

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Effect of sidaguri and jatropha on tooth microhardness and tooth surface: An in vitro evaluation using CLSM Maria Tanumiharja; Nurhayaty Natsir; Indrya K. Mattulada; Hafsah Katu; Harun Achmad; Lukman Muslimin; Haerul Arsyad; Lukmanul H. Arma
Journal of Dentomaxillofacial Science Vol. 6 No. 2 (2021): (Available online: 1 August 2021)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v6i2.1219

Abstract

Objective: The purpose of this study was to evaluate the effect of herbal alternatives, extract of sidaguri (sida rhombifolia) combine with extract of jatropha sap (jatropha curcas) application on microhardness and morphology of tooth surface. Material and Methods: Twelve upper first premolar teeth were used in this study, prepared to Class I box cavities, and were then allocated into 4 groups (n=3). Group 1-3 were treated groups, applied with combined extracts of a small dose (5 mg), medium dose (25 mg), and high dose (50 mg), while group 4 was the control group. The cavities were restored with composite resin, immersed in artificial saliva, and stored at room temperature for 14 days. The fillings and extracts were removed and cleaned under running tap water. The teeth were cut in a vertical direction towards the apex, sectioned into 1 mm thickness, and evaluated using Laser Scanning Microscope (LSM), then were examined for microhardness test. Results: All treated groups showed significantly lower microhardness than the control group. Tooth porosity was observed, and its extensiveness was affected by doses of extracts. Conclusion: The application of herbal alternatives (extract of sidaguri and jatropha) lowered the microhardness of tooth structure and caused tooth porosities.
Dentin microhardness and porosity of wistar rats teeth following herbal-based devitalizing agent application Maria Tanumiharja; Mustakim Mustakim; Aries C. Trilaksana; Lukman Muslimin
Journal of Dentomaxillofacial Science Vol. 7 No. 2 (2022): (Available online: 1 August 2022)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v7i2.1351

Abstract

Objective: To evaluate the microhardness and porosity of the dentin of Wistar rats after pulp-out application. Materials and Methods: This was laboratory experimental research with posttest design with control group. Twelve teeth of wistar rats were allocated into 2 groups, the test group and the control group. In the test group, teeth were prepared on the labial surface until half thickness of the dentin, then pulpout was applied to the base of cavity, covered with GIC. On the 7th day, euthanasia and extraction were performed to test the dentin microhardness with Vickers Hardness Tester and dentin porosity with Laser Scanning Microscope (LSM). Results: The mean value of tooth hardness after pulp-out application was 22.23 ± 3.51 HV while the mean value of the control group was 27.70 ± 1.70 HV, with p value = 0.31 at 95% significance level. There was a decrease in tooth hardness of treated sample which was not significantly different compared to the control group. CLSM photo shows white lines on the sample surface as the exposed dentinal tubules with a higher intensity compared to the control group. Conclusion: Pulp-out decreased tooth microhardness and cause porosity of the teeth.