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Nurhayaty Natsir
Department of Conservative Dentistry, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia
Dentistry

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Calcium loss on root canal dentin after Ethylenediaminetetraacetic acid (EDTA) application with different varians Juni J. Nugroho; Christine A. Rovani; Hermiati Daharuddin; Aries C. Trilaksana; Nurhayaty Natsir; Andi Sumidarti
Journal of Dentomaxillofacial Science Vol. 4 No. 2 (2019): (Available online: 1 August 2019)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1356.336 KB) | DOI: 10.15562/jdmfs.v4i2.781

Abstract

Objective: This study aimed to compare calcium loss on root canal dentin before and after EDTA application with different varians and concentration with or without surfactant.Material and Methods: Samples are mandibular premolar teeth single root canal, which is divided into 4 groups: Ethylenediaminetetraacetic Acid (EDTA) gel, cream, solution and negative control. They are decoronated in Cemento-Enamel Junction (CEJ), then prepared with Crown Down Pressureless (CDP) technique and separated bucolingual direction. One side was applied in EDTA according their respective groups and the other side was set as sample before application. Samples before and after application of each group are destructed then subsequently measured for their Ca2+’s amount using Atomic Absorption Spectrophotometry (AAS). Data were collected and analyzed using ANOVA and Tukey Post Hoc test.Results: This showed significant differences between Ca2+ amount in root canal dentin before and after application either on EDTA gel, cream, or solution as well as on negative control group (p<0.05).Conclusion: Application of EDTA solution causes the highest calcium loss compared to EDTA gel and cream.
The increase of fibroblast cells number in rat (rattus norvegicus) gingival wound after the application of moringa (moringa oleifera lam) fruit oil Asmawati; Bahruddin Thalib; Nurhayaty Natsir; Alqarama M. Thalib; Rafikah Hasyim
Journal of Dentomaxillofacial Science Vol. 5 No. 3 (2020): (Available online: 1 December 2020)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v5i3.1121

Abstract

Objective: To determine the increase of fibroblast cell number in the rat (Rattus norvegicus) gingival wound after the application of moringa (Moringa oleifera Lam) fruit oil.Material and Methods: This study was an experimental post-test control group design using 18 male Wistar rats (Rattus norvegicus) that was divided into three groups. The sample was previously incised with a length of 4 mm using scalpel 15. Group 1 was applied with moringa fruit oil, group 2 was given aspirin, and group 3 was given at 0.5% CMC. The application was carried out every 24 hours, then on the third and seventh day, the rats were euthanized for histological preparations and then observed under the electric microscope. The length of the lesion was measured on the first, third, and seventh days. The fibroblast was then observed by counting the number of fibroblast cells per field of view using an Olympus C-21 microscope with an Optilab Advances camera with a 40x, 100x magnification, and 400x. The data were processed and analyzed using the SPSS Version 21 program. This study used a t-test to see the differences between the treatment group and the control group, and the Least Significantly Difference (LSD) test was used to see the size of the difference.Results: This study showed a significant increase in the number of fibroblasts in rat gingival mucosa on the third and seventh daysConclusion: Moringa (Moringa oleifera Lam) fruit oil affects increasing the number of fibroblasts on the rat gingival mucosa (Rattus norvegicus). 
Effect of sidaguri and jatropha on tooth microhardness and tooth surface: An in vitro evaluation using CLSM Maria Tanumiharja; Nurhayaty Natsir; Indrya K. Mattulada; Hafsah Katu; Harun Achmad; Lukman Muslimin; Haerul Arsyad; Lukmanul H. Arma
Journal of Dentomaxillofacial Science Vol. 6 No. 2 (2021): (Available online: 1 August 2021)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v6i2.1219

Abstract

Objective: The purpose of this study was to evaluate the effect of herbal alternatives, extract of sidaguri (sida rhombifolia) combine with extract of jatropha sap (jatropha curcas) application on microhardness and morphology of tooth surface. Material and Methods: Twelve upper first premolar teeth were used in this study, prepared to Class I box cavities, and were then allocated into 4 groups (n=3). Group 1-3 were treated groups, applied with combined extracts of a small dose (5 mg), medium dose (25 mg), and high dose (50 mg), while group 4 was the control group. The cavities were restored with composite resin, immersed in artificial saliva, and stored at room temperature for 14 days. The fillings and extracts were removed and cleaned under running tap water. The teeth were cut in a vertical direction towards the apex, sectioned into 1 mm thickness, and evaluated using Laser Scanning Microscope (LSM), then were examined for microhardness test. Results: All treated groups showed significantly lower microhardness than the control group. Tooth porosity was observed, and its extensiveness was affected by doses of extracts. Conclusion: The application of herbal alternatives (extract of sidaguri and jatropha) lowered the microhardness of tooth structure and caused tooth porosities.
Potential of moringa fruit (moringa aloifera lamk) seeds as an anti-inflammatory agent of oral cavity lesion Asmawati; Bahruddin Thalib; Nurhayaty Natsir; Fajriani; Alqarama M. Thalib; Delvi S. Reni
Journal of Dentomaxillofacial Science Vol. 6 No. 2 (2021): (Available online: 1 August 2021)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v6i2.1231

Abstract

Objective: To know the potential of moringa fruit seed extract (moringa Aloifera Lamk) as an anti-inflammatory agent of oral cavity lesion.Methods: One of the natural ingredients that are widely obtained in South Sulawesi is moringa plants and from several researches this plant has been proven to have a variety of properties. Moringa fruit extracts contain components in the form of flavonoids, saponins and tannins. The content serves as an antibacterial and anti-inflammatory. Wound is the loss or partial damage of tissues on the body, one form of wounds that occur in the gingival is a lesion due to irritation, traumatic wounds or wounds due to preoperative incision in the extraction of impacted teeth.Results: Therefore, a natural ingredient that is easily obtained at a low price and not a b-ingredient that is made from chemicals that will provide negative effects such as hypersensitivity and resistance for those who use these drugs.Conclusion: Moringa Oleifera Lamk seed oil has an effect on the formation of collagenase and an increase in the amount of fibroblast so that it has the potential to become an anti-inflammatory drug of the oral cavity.
Co-Authors Alqarama M. Thalib Alqarama M. Thalib Andi Sumidarti Aries C. Trilaksana Asmawati Bahruddin Thalib Christine A. Rovani Delvi S. Reni Fajriani Haerul Arsyad Hafsah Katu Harun Achmad Hermiati Daharuddin Indrya K. Mattulada Juni J. Nugroho Lukmanul H. Arma Maria Tanumiharja Rafikah Hasyim