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All Journal Journal of Dentomaxillofacial Science
Aries C. Trilaksana
Department of Conservative Dentistry and Endodontics, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia
Dentistry

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Effect of pup out® on caspase 3 and interleukin-1B expression in pulp teeth: a paste contained jatropha, sidaguri, and melittin Maria Tanumiharja; Arfina S. Hamid; Aries C. Trilaksana; Christine A. Rovani; Nurhayati Natsir; Juni J. Nugroho; Indrya K. Mattulada; Lukman Muslimin
Journal of Dentomaxillofacial Science Vol. 6 No. 2 (2021): (Available online: 1 August 2021)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v6i2.1232

Abstract

Objective: To examine the expression of caspase-3 and IL-1? following application of Pulp Out® on rabbit’s pulp teeth as well as histopathology.Material and Methods: The study was conducted on the maxillary incisors rabbits. The teeth were prepared and Pulp Out® was inserted at the base of prepared cavity, then restored with RM-GIC. After 24 hours, the animals were then euthanized and processed for histopathology and immunohistochemistry evaluation.Results: Our results indicated that Pulp Out®, when administered to the pulp teeth, increased the number and diameter of blood vessels. The expression of caspase-3 and IL-1? showed similarities to the expression of commercial devitalizing agent.Conclusion: Our results revealed that Pulp Out® is a valuable devitalization agent that should be further explored for its safety before clinical application.
Dentin microhardness and porosity of wistar rats teeth following herbal-based devitalizing agent application Maria Tanumiharja; Mustakim Mustakim; Aries C. Trilaksana; Lukman Muslimin
Journal of Dentomaxillofacial Science Vol. 7 No. 2 (2022): (Available online: 1 August 2022)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v7i2.1351

Abstract

Objective: To evaluate the microhardness and porosity of the dentin of Wistar rats after pulp-out application. Materials and Methods: This was laboratory experimental research with posttest design with control group. Twelve teeth of wistar rats were allocated into 2 groups, the test group and the control group. In the test group, teeth were prepared on the labial surface until half thickness of the dentin, then pulpout was applied to the base of cavity, covered with GIC. On the 7th day, euthanasia and extraction were performed to test the dentin microhardness with Vickers Hardness Tester and dentin porosity with Laser Scanning Microscope (LSM). Results: The mean value of tooth hardness after pulp-out application was 22.23 ± 3.51 HV while the mean value of the control group was 27.70 ± 1.70 HV, with p value = 0.31 at 95% significance level. There was a decrease in tooth hardness of treated sample which was not significantly different compared to the control group. CLSM photo shows white lines on the sample surface as the exposed dentinal tubules with a higher intensity compared to the control group. Conclusion: Pulp-out decreased tooth microhardness and cause porosity of the teeth.
Co-Authors Arfina S. Hamid Christine A. Rovani Indrya K. Mattulada Juni J. Nugroho Maria Tanumiharja Maria Tanumiharja Mustakim Mustakim Nurhayati Natsir