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All Journal Jurnal Sain Veteriner Jurnal Veteriner International Journal of Science and Engineering (IJSE) Bioma : Berkala Ilmiah Biologi Prosiding Seminar Biologi Pro-Life Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology)
SRI ISDADIYANTO
Departemen Biologi Fakultas Sains Dan Matematika Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : Pro-Life

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HEPATOSOMATIK INDEKS DAN DIAMETER HEPATOSIT MENCIT (Mus musculus L.) BETINA SETELAH PAPARAN EKSTRAK ETANOL DAUN MIMBA (Azadirachta indica Juss.) Anisa Nurul Hasana; Agung Janika Sitasiwi; Sri Isdadiyanto
Jurnal Pro-Life Vol. 6 No. 1 (2019): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Neem leaves is one part of the neem tree which can be used as a traditional medicinal herb. The purpose of this study was to examine the effect of neem leaf ethanol extract on hepatosomatic index (HSI) and hepatocyte diameter in female mice. The study used a Complete Randomized Design consist of 4 treatments and 5 replications including K (aquadest), P1, P2 and P3 (neem leaf ethanol extract with dose of 8.4,11.2 and 14 mg/ kg BW/day. The treatment was administered orally with a volume of 0.2 mL for 21 days. Body weight were measured every 7 days. The liver was isolated, weighed and histologically prepared with paraffin method, HE staining and 5 μm incision thickness. HSI value determined from body weight and liver weight. Hepatocyte diameter were measured on 7 hepatocytes per lobule. The data was analyzed using ANOVA at 95% confidence level. The results showed that neem leaf ethanol extract had no significant effect (p> 0,05) on body weight, liver weight, HSI value and hepatocyte diameter, but the liver microanatomy showed hepatocyte damage. It can be concluded that exposure of neem leaf ethanol extract for 21 days still safe to be used for reference of traditional medicine. Keywords: Neem leaf, HSI, hepatocyte diameter
STRUKTUR MIKROANATOMI GINJAL MENCIT (Mus Musculus L.) BETINA SETELAH PAPARAN EKSTRAK ETANOL DAUN MIMBA (Azadirachta indica A. Juss) Elsa Billah Septiva; Agung Janika Sitasiwi; Sri Isdadiyanto
Jurnal Pro-Life Vol. 6 No. 2 (2019): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Neem leaves (Azadirachta indica A. Juss.) contain organic compounds such as alkaloids, steroids, flavonoids, triterpenoids, saponins and tannins which have any potency as traditional medicinal. This study aimed to examine the ethanolic extract of Neem leaves on kidney microanatomy in mice (Mus musculus L.). Completely Random Design consists of 4 treatments, 5 replications, namely K (aquadest), P1, P2 and P3 (Treated by dosages 8.4; 11.2 and 14 mg/kg BB/day). The treatments were given orally with volume 0.2 mL for 21 days. Kidney organ was isolated, weighed and made by histological processed with a 5 μm incision thickness using the paraffin method with Hematoxylin-Eosin staining. Observation of preparations were carried out to calculate glomerular diameter, Bowman’s capsule diameter and Bowman’s space diameter. The data were analyzed using ANOVA test at 95% confidence level and continued with DMRT test. The analysis results showed that there was significant affect (p<0.05) on exposure ethanolic extract of Neem leaves to the glomerular and Bowman's capsule diameter but didn’t show significantly affect (p>0.05) to the Bowman's space diameter, changes in kidney weight and drinking consumption of mice. It could be concluded that the exposure of ethanolic extract of Neem leaves in a period of 21 days is still safe to use as traditional alternative medicine. Keywords: Glomerular diameter, kidney weight, Neem leaves
Histopathology of Rats Intestinal Treated with High-Fat Diet and Neem Leaf Extract Azmi Syafanah Nur Hasna; Sri Isdadiyanto; Agung Janika Sitasiwi
Jurnal Pro-Life Vol. 9 No. 1 (2022): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

The high-fat diet can increase free radicals, which can damage the intestinal histological structure. The ethanolic neem leaf extract contains antioxidants that have the potential to help improve the intestinal histological structure. This study aims to examine the effectiveness of ethanolic neem (A. indica A. Juss) leaf extract on the histopathological structure of the small intestine in rats (R. norvegicus L.) hyperlipidemia. This study used 24 rats divided into 6 groups: normal control, positive control, high-fat diet + simvastatin, the test groups dose I, II, and III (ethanolic neem leaf extract 75 mg/200 gBW, 100 mg/200 gBW, and 125 mg/200 gBW). The results of the analysis using ANOVA test on the height of villi, mucous thickness, and number of goblet cells in the small intestine part duodenum, jejunum, and ileum showed no significant difference (p>0.05). The results of the morphology of epithelial cells in treatments P0, P2, and P5 showed normal epithelial cells, whereas treatments P1, P3, and P4 showed erosion epithelial cells. Based on the result, the ethanolic neem (A. indica A. Juss) leaf extract can suppress the damage effects of epithelial cells and the histological structure of the small intestine in rats (R. norvegicus L.) hyperlipidemia
Co-Authors Agung Janika Sitasiwi Agung Janika Sitasiwi Agung Putra Nugraha Anggih Retno Pratiwi Anisa Nurul Hasana Ayu Dwi Lestari Azmi Syafanah Nur Hasna Citra Riandika Didik Setiyo Widodo Eka Fitriani Eka Wahyu Cahya Ningrum Elsa Billah Septiva Fani Fahriyansyah Fauziah Mustika Putri Fitra Nilla Sari Ginanjar Argo Pambudi Ika Oktavia Nurmila Kasiyati Kasiati khabibi Khabibi Luthfiana Ulil Albab Luthfiana Ulil Albab Mohammad Farhan Aditya Muhammad Anwar Djaelani Muhammad Farizan Adani Nunuk Shofiati Ratih Nur Fitriani Rd Yudhi Tresnadi Kusumah Retno Ariadi Lusiana Silvana Tana Siti Muflichatun Mardati Siti Muflichatun Mardiati Siti Muflichatun Mardiati Siti Nur Jannah Sunarno Sunarno Tazkia Annisa Tyas Rini Saraswati Winda Arini