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All Journal Jurnal Sain Veteriner Jurnal Akademika Biologi Pro-Life E-Dimas: Jurnal Pengabdian kepada Masyarakat Jurnal Biologi Tropika Indonesia Journal of Halal NICHE Journal of Tropical Biology Jurnal Surya Masyarakat Scripta Biologica To Maega: Jurnal Pengabdian Masyarakat Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Surya Masyarakat Prosiding Konferensi Nasional PKM-CSR Jurnal Pengabdian Vokasi Jurnal Teknologi Pangan E-Amal: Jurnal Pengabdian Kepada Masyarakat

Siti Nur Jannah

Biotechnology Laboratory, Departement Of Biology, Faculty Of Science And Mathematics, Diponegoro University

Author-ID : 3640403

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mechanical Engineering Nursing Public Health Social Sciences Veterinary Other

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Studi Histopatologi Ren Tikus Putih (Rattus Norvegicus L.) Diabetes Setelah Pemberian Cuka dari Kulit Nanas (Ananas Comosus (L.) Mer.) Tazkia Annisa; Agung Janika Sitasiwi; Sri Isdadiyanto; Siti Nur Jannah
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.56891

Diabetes mellitus is a metabolic disease that occurs due to impaired insulin secretion caused by progressive damage to beta cells. Pineapple skin vinegar contained acetic acid and antioxidants which have the potential to help repaired the structure of the nephron ren and other organs affected by diabetes. The purpose of this study was to examined the effectiveness of pineapple skin vinegar on improved the histological structure of diabetic rats ( Rattus norvegicus L.). This study based on changed in the structure of the nephron in samples of normal and alloxan-induced mice pre-treatmented and post-treatmented. Twenty-four rats were divided into 6 groups, named normal control, positive control (diabetes + 0.4 mL apple vinegar), negative control (diabetes + water), dose test groups 1, 2, and 3 (pineapple vinegar 0.2 mL; 0.4 mL; 0.8 mL). Statistical analysis test used ANOVA was followed by Duncan test. The conclusion of this research, the pineapple skin vinegar showed the ability to repair the histopathological structure of white rats damaged by diabetes. The optimum dose needed was 0.8 mL to improved the histological structure of the nephron, as indicated by the glomerular diameter and the distance of the Bowman's capsule space to near normal.

Isolasi dan Aktivitas Antikapang Bakteri Asam Laktat dari Tape Ketan Kemasan Plastik terhadap Fusarium sp. Khoirin Nisa; Siti Nur Jannah; Isworo Rukmi
Jurnal Akademika Biologi Vol. 9 No. 2 Juli 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (462.649 KB)

Fusarium sp. merupakan patogen tular tanah yang memiliki kemampuan bertahan dalam tanah selama bertahun-tahun, pada kondisi lingkungan yang tidak menguntungkan. Bakteri asam laktat (BAL) diketahui menghasilkan senyawa antikapang yang dapat digunakan sebagai salah satu alternatif pengendalian Fusarium sp. yang ramah lingkungan. BAL dapat ditemukan dalam produk fermentasi, antara lain pada tape ketan kemasan plastik. Tujuan penelitian ini adalah untuk mendapatkan isolat murni bakteri asam laktat (BAL) dari tape ketan kemasan plastik dan mengetahui aktivitas antikapang isolat BAL yang yang diperoleh dalam menghambat pertumbuhan kapang Fusarium sp. Penelitian ini menggunakan kultur BAL, supernatan BAL bebas sel (CFS) dan supernatan bebas sel BAL yang dinetralkan (CFSN). Uji aktifitas antikapang dilakukan dengan metode sumuran pada medium MRSA. Hasil penelitian menunjukkan bahwa dari tape ketan kemasan plastik diperoleh enam isolat BAL (BTP1, BTP2, BTP3, BTP4, BTP5, BTP6). Lima isolat BAL (BTP1, BTP2, BTP3, BTP4, BTP5) mampu menghambat pertumbuhan kapang Fusarium sp. dengan aktivitas antikapang fungistatik. Supernatan bebas sel BAL yang dinetralkan (CFSN) BTP1menunjukkan aktivitas antikapang yang paling besar dan berbeda nyata dengan isolat BAL lainnya.

STUDI HISTOLOGI PANKREAS TIKUS PUTIH (Rattus norvegicus L.) SETELAH PEMBERIAN CUKA DARI KULIT NANAS (Ananas comosus L. Merr) Christin Monica Hermawati; Agung Janika Sitasiwi; Siti Nur Jannah
Jurnal Pro-Life Vol. 7 No. 1 (2020): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Diabetes mellitus is a metabolic disease that occurs due to impaired insulin secretion caused by progressivedestruction of beta cells. Pineapple peel vinegar containing acetic acid and antioxidants has the potential to helpimprove the structure of beta cells in the Langerhans islet. This study aims to examine the effectiveness of pineapplepeel vinegar towards the histological improvement of Langerhans islet in diabetic rats. Twenty-four rats weredivided into 6 groups, normal control, positive control (diabetes + 0,4 ml apple vinegar), negative control(diabetes + water), test groups dose I, II, and III (0,2 ml; 0,4 ml; 0,8 ml pineapple vinegar). The treatment groupthat given 0.4 ml pineapple peel vinegar showed improvement in islet's structure as indicated by its size andnumber that close to normal. The results of the statistical analysis using Kruskal-Wallis followed by Mann-Whitneyshowed that the diameter of the Langerhans islet in group P2 (treated by 0,4 ml pineapple peel vinegar) was closeto normal. The improved structure of the Langerhans islet resulted in a decrease in blood sugar levels by 41,63%.This percentage is still lower than the K+ group (treated by 0,4 ml apple vinegar) by 52,89%. The antioxidantactivity in vinegar improves the structure of the Langerhans islet by binding to free radicals that cause damage.The acetic acid in vinegar inhibits disaccharidase activity so that it can control blood sugar levels.Keywords: Diabetes mellitus, alloxan, pineapple peel vinegar, antioxidants, acetic acid

UJI AKTIVITAS ANTIDIABETES CUKA KULIT NANAS (Ananas comosus L. Merr.) PADA TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR WISTAR YANG DIINDUKSI ALOKSAN Mohammad Affan Dwica Putra; Siti Nur Jannah; Agung Janika Sitasiwi
Jurnal Pro-Life Vol. 7 No. 2 (2020): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Diabetes mellitus is a metabolic disorder characterized by increased levels of glucose in the blood (hyperglycemia). The increasing number of diabetics in the world encourages the discovery of certain drugs that are effective and relatively safe for managing diabetes. One alternative medicine that can be used is vinegar. Pineapple skin can be an alternative raw material for making vinegar because it contains a large amount of sugar and contains acetic acid and various secondary metabolites that can reduce blood glucose levels. The purpose of this study was to determine whether pineapple skin vinegar has antidiabetic activity. The test was carried out by dividing each of the 4 rats into six groups namely normal control, positive control, negative control, and three test groups that were given pineapple skin vinegar as much as 0.2, 0.4, and 0.8 cc. The treatment was carried out for 28 days, starting 4 days after induction of an alloxan dose of 150 mg / kg body weight. Measurement of blood glucose and body weight of rats were carried out before induction, after induction, and on the 7th, 14th, 21st, and 28th day, while the measurement of feed consumption was carried out every day starting from the day of pineapple skin vinegar administration. The results of the analysis showed that the administration of pineapple skin vinegar by 0.4 cc with acetic acid levels of 1.43% can reduce blood glucose levels most significantly that is 41.63%.Keywords: vinegar, pineapple skin, acetic acid, antidiabetic

Produksi Serbuk Jamu Instan dengan Alat Kristalisasi di UMKM Kecamatan Ungaran Timur Kabupaten Semarang Susiana Purwantisari; Siti Nur Jannah; Dwi Handayani; Muhammad Endy Yulianto; Anindya Ardiansari
E-Dimas: Jurnal Pengabdian kepada Masyarakat Vol 12, No 3 (2021): E-DIMAS
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/e-dimas.v12i3.7070

Serbuk jamu merupakan obat tradisional berbentuk serbuk berisi seluruh bahan tanaman yang menjadi penyusun jamu tersebut yang disediakan dan digunakan secara tradisional. Terdapat 2 buah UMKM yaitu UMKM “Tiga Dara” dan UMKM “Berliana” yang produktif secara ekonomis dengan memproduksi berupa serbuk jamu sebagai minuman kesehatan. Serbuk jamu instan yang diproduksi oleh UMKM Tiga Dara dan UMKM Berliana meliputi serbuk instan jahe, kunyit, temulawak, mahkota dewa, kencur, kunir putih, kunir asem, dll. Selama ini proses produksi serbuk jamu instan, dilakukan secara manual dan konvensional sehingga membutuhkan banyak tenaga serta hasil produksinya terbatas. Oleh karenanya perlu adanya mekanisasi khususnya pada pengkristalan serbuk jamu instan tersebut. Program ini bertujuan untuk meningkatkan kualitas dan efektivitas produksi serbuk jamu instan yang tergabung dalam UMKM ”Tiga Dara” dan UMKM ”Berliana” melalui produksi serbuk jamu instan menggunakan alat kristalisasi. Metode yang digunakan untuk mencapai tujuan program adalah pelatihan produksi serbuk jamu instan dengan alat kristalisasi, membuat standar operasional proses (SOP) produksi , membuat SOP sanitasi dan hygiene produksi serbuk jamu instan serta SOP keselamatan dan kesehatan kerja. Hasil dari kegiatan ini adalah tercapainya keterampilan kedua UMKM dalam mengelola empon-empon menjadi produk serbuk jamu instan, higienis yang sesuai persyaratan BPOM dan telah mempunyai SOP produksi serbuk jamu instan dengan alat kristalisasi, SOP sanitasi dan hygiene produksi serbuk jamu instan serta SOP keselamatan dan kesehatan kerja.

Kualitas mikrobiologi daging ayam broiler di pasar tradisional Banyumanik Semarang Widayu Mutiya Ramadhani; Isworo Rukmi; Siti Nur Jannah
Jurnal Biologi Tropika Vol. 3, No. 1, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Broiler chicken meat is a a kind of food that is easily spoilage due to bacterial growth. The chicken meat sold in the market could potentially become a medium of microbial growth of foodborne diseases. The aims of this research was to determined the microbiological quality of chicken meat sold at traditional market in Banyumanik, Semarang City and examined the source of bacterial contaminant especially E. coli on chicken meat. Samples were taken from two markets in Banyumanik, namely Dm Market and Jt Market of Semarang. The samples were purposive taken, from two different sellers in each market. Sampling was conducted at 08.00 AM and 10.00 AM. The parameters in this study are the total number of bacteria, the number of E. coli and the presence of Salmonella sp. The results showed that all samples examined did not meet the standard of good quality microbiological requirements for chicken meat according to SNI 3924:2009, regarding total bacteria, E. coli content and the presence of Salmonella sp. The results of observations by contact plate technique found that 50% of tools used by chicken meat sellers were contaminated by E. coli.

Seleksi dan Identifikasi Secara Molekuler Bakteri Pendegradasi Insektisida Piretroid dari Tanah Salindri Prawitasari; Siti Nur Jannah; Alina Akhdiya
Indonesia Journal of Halal Vol 1 (1) 2018
Publisher : Pusat Kajian Halal Undip

ABSTRAKAkumulasi residu insektisida pada lahan pertanian berdampak negatif bagi lingkungan dan organisme di sekitarnya. Salah satu teknologi alternatif untuk merehabilitasi lahan pertanian yang tercemar adalah dengan teknologi bioremediasi. Bioremediasi adalah teknologi untuk memecah atau menguraikan zat pencemar menjadi bahan yang kurang beracun atau tidak beracun (karbondioksida dan air) dengan memanfaatkan organisme atau produk organisme. Penelitian ini bertujuan untuk menyeleksi dan mengidentifikasi secara molekuler bakteri pendegradasi insektisida piretroid sintetik asal sampel tanah Pangalengan. Seleksi terhadap sembilan isolat bakteri tanah asal Pangalengan menggunakan medium NMS cair yang mengandung 100 ppm piretroid menghasilkan dua isolat bakteri yang memiliki kemampuan terbaik dalam mendegradasi piretroid. Isolat S-9 merupakan isolat bakteri yang memiliki kemampuan mendegradasi residu piretroid paling tinggi dibandingkan dengan 8 isolat lainnya, yaitu sebesar 87,38%. Hasil degradasi insektisida tersebut dimanfaatkan oleh bakteri sebagai sumber C, N, dan P untuk mendukung pertumbuhannya. Berdasarkan sekuens gen 16S rRNA, isolat S-9 menunjukkan kemiripan dengan Bosea eneae 16S ribosomal RNA gene (partial sequence) dengan similaritas 89%.Kata kunci: bakteri, bioremediasi, degradasi insektisida, piretroid ABSTRACTThe accumulation of insecticide residue in farmfield caused negative impact to the environment and organisms which surround it. One of the lastest technology to rehabilitate polluted farmfield is bioremediation technology. Bioremediation is technology for degradating pollute to be unpollute (carbondioxide and water) using microorganisms or its product. The purpose of this research was to selecting and molecular identifying pyrethroid insecticide-degradating bacteria from Pangalengan soil sample. Nine isolates of soil bacteria from Pangalengan was selected by liquid NMS contains 100 ppm pyrethroid, only 2 isolates that indicated best ability to degrade pyrethroid. The best ability to degrade pyrethroid was S-9 isolate, with highest value 87,38%. Degradation pyrethroid’s product utilized by the bacteria as C, N, and P source to support their growth. Based on 16S rRNA sequence gene, S-9 isolate represented its similarity with Bosea eneae 16S ribosomal RNA gene (partial sequence) with 89% similarity value.Keywords: bacteria, bioremediation, insecticide degradation, pyrethroid

Isolasi bakteri asam laktat dari tape ketan dan potensinya sebagai agen antikapang terhadap pertumbuhan Aspergillus flavus Peni Koriasih; Siti Nur Jannah; Budi Raharjo
NICHE Journal of Tropical Biology Vol. 2, No. 2, Year 2019
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Isolation and characterization of lactic acid bacteria from Apis mellifera and their potential as antibacterial using in vitro test against growth of Listeria monocytogenes and Escherichia coli Indriana Putri; Siti Nur Jannah; Susiana Purwantisari
NICHE Journal of Tropical Biology Vol. 3, No. 1, Year 2020
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Food spoilage can caused by pathogenic bacteria and can cause disease (food-borne pathogens). Foods that are vulnerable to pathogenic bacteria are foods that contain lots of protein, such as meat, milk and eggs. Listeria monocytogenes and Escherichia coli are commonly found in fresh meat. The solution for the problem is microbiological preservation by using Lactic Acid Bacteria (LAB). The purpose of this study is to isolate LAB from Apis mellifera honey, find out its characteristics, and test for antibacterial growth of L. monocytogenes and E. coli. LAB have been isolated with pour plate. Morphological characterization have been done by observing the colonies macroscopically and microscopically. LAB have been enumerated by Total Plate Count (TPC). Antibacterial Test have been done by well diffussion method. Experimental design was using Completely Randomized Factorial Design (CRFD). Statistical analysis have been done by univariate test with SPSS 16.0. 1st factor is LAB, 2nd factor is pH of supernatant and 3rd factor is pathogenic bacteria. Total 9 isolates of LAB were obtained from A. mellifera honey. LAB population is 9.6 x 105 CFU/mL. The result of screening LAB antibacterial assay is 2 potential isolates, they are MA 3 and MA 4. The best supernatant of LAB that could inhibit the growth of L. monocytogenes and E. coli is MA 3 with average diameters 12,05 mm and 12,07 mm. Pathogenic bacteria that is more sensitive is E. coli, because of the antibacterial compound from LAB is composed by organic acid that can break down the outer membrane and lower the pH, so pathogenic bacterial cannot be adapted to acidic environment.

Pengaruh penambahan sumber karbon terhadap produksi antibakteri isolat endofit A1 tanaman ciplukan (Physalis angulata L.) terhadap Escherichia coli dan Staphylococcus aureus Hendrawati Retno Wulandari; Sri Pujiyanto; Siti Nur Jannah
NICHE Journal of Tropical Biology Vol. 3, No. 2, Year 2020
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.3.2.80-88

Co-Authors Agung Janika Sitasiwi Alina Akhdiya Ana Bila Nadia Anindya Ardiansari Annisa Dhea Lathifa Annisa Yulina Susilowati Bodhicitta Wardaja Budi Raharjo Budi Setiyana Budi Setiyana Christin Monica Hermawati Daffa Ikhlasul Amal Dwi Handayani Dzakiyya Nabilla Fikri Eflita Yohana Hendrawati Retno Wulandari Hermawan Dwi Ariyanto Hermawan Dwi Ariyanto Hermin Pancasakti Kusumaningrum Hermin Pancasakti Kusumaningrum Ilyas Teguh Pangestu Indriana Putri Khoirin Nisa MG Isworo Rukmi Mohamad Endy Y Yulianto Mohammad Affan Dwica Putra Peni Koriasih Rejeki Siti Ferniah Rizky Wahyu Saputro Salindri Prawitasari SRI ISDADIYANTO Sri Pujiyanto Sri Pujiyanto Sri Rustini Sukma Aulia Wulandari Sulistiani Sulistiani Sulistiani Susiana Purwantisari Tazkia Annisa Widayu Mutiya Ramadhani Yunior William Susanto

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