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All Journal BERITA BIOLOGI JFMR (Journal of Fisheries and Marine Research)
Des Roza
Balai Besar Riset Perikanan Budidaya Laut Gondol
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Engineering Environmental Science

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EFEKTIFITAS METODA VAKSINASI FLEXIBACTER PADA BENIH IKAN KERAPU MACAN, EPINEPHELUS FUSCOGUTTATUS DI HATCHERI Johnny, Fris; Roza, Des; Zafran, Zafran
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.695

Abstract

Vaccination is one of the approaches against infectious diseases in cultured marine fish. The purpose of this study was to evaluate the effectiveness of Flexibacter vaccine in juvenile of tiger grouper (Epinephelus fuscoguttatus). Nine hundred of tiger grouper with total length of 7-8 cm reared in nine concrete tanks. For each three tanks, fishes were vaccinated with injection, immersion, and without vaccination as control. Booster was delivered at 10 and 30 days after first vaccination. Challenge test with live Flexibacter bacteria was performed 90 days post vaccination. Observations were made on clinical symptoms and survival rates which presented in relative percentage survival(RPS).The experiments were performed in completely randomized design with three replicates. The data then analyzed with ANOVA. The results showed that the antibody titer values of vaccinated fish were higher then unvaccinated fish, namely 128 for injection, 64 for bathing and 4 for control. Survival rates of vaccinated fish following challenged test were higher than control and significantly different (P<0.05), i.e. 88.67% (with RPS of 60%) for injection and 83.33% (with RPS of 50%) for immersion and 66.67% for controls. It is suggested that Flexibacter vaccine administered through injection and bathing are effective to increase immunity of tiger grouper against Flexibacter infection
INFEKSI BAKTERI VIBRIO ALGINOLYTICUS PADA LUMBA-LUMBA HIDUNG BOTOL, TURSIOPS ADUNCUS YANG DIPELIHARA DI LOVINA, SINGARAJA, BALI Johnny, Fris; Roza, Des
BERITA BIOLOGI Vol 13, No 3 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i3.673

Abstract

An experiment with the aim to identify the cause of disease in Indo-Pacific bottlenose dolphins, Tursiops aduncus was conducted in Pathology Laboratory of Institute for Mariculture Research and Development, Gondol, Bali. The diseased fish showed lost appetite, swim slowly, and haemorrhage on the body surface. These clinical signs indicate that the fish infected by bacteria. The bacteria then were isolated aseptically from different parts of fish body including chin, abdomen, dorsal and caudal fins. Some media were used to isolate the bacteria, namely Triptic Soy Agar (TSA) as a general medium, Thiosulphate Citrate Bile Salt Sucrose Agar (TCBSA) for Vibrio bacteria, Cytophaga Agar (Cyt-A) for Flexibacter bacteria, and KF-Strep Breeders media for Streptococcus bacteria. As a result, one dominant bacterium was isolated from TSA and TCBSA. No bacteria growth showed on Cyt-A and KF-Strep media.The isolate was gram-negative, fermentative, swarm on TSA, growth with yellow colony on TCBSA. Based on its characteristics the isolate identified as Vibrio alginolyticus. Minimum inhibitory concentration (MIC) against Vibrio alginolyticus were 1 ppm for Nifurpirinol, 5 ppm for Penstrep, and 10 ppm or Elbaju.
IDENTIFIKASI BAKTERI PADA LARVA IKAN TUNA SIRIP KUNING (Thunnus albacares) Mastuti, Indah; Setiadi, Ananto; Roza, Des; Mahardika, Ketut
JFMR (Journal of Fisheries and Marine Research) Vol. 4 No. 1 (2020): JFMR
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2020.004.01.1

Abstract

Perbenihan tuna sirip kuning (Thunus albacares) telah dilakukan sejak tahun 2015 di Balai Besar Riset Budidaya Laut dan Penyuluhan Perikanan, Gondol, Bali. Akan tetapi, dalam perbenihan tuna sirip kuning masih dihadapkan adanya tingkat kematian yang tinggi pada tahap larva. Penelitian ini bertujuan untuk mengidentifikasi bakteri pada perbenihan larva tuna sirip kuning. Bakteri diisolasi dari larva umur 5, 10, 15, 20 hari setelah menetas pada media marine agar. Koloni bakteri yang tumbuh direisolasi berdasarkan morfologinya. Setiap isolat diidentifikasi secara molekuler berdasarkan urutan nukleotida dalam gen ribosom 16S menggunakan pasangan primer 27F dan 1492R. Kesamaan masing-masing urutan nukleotida dianalisis dengan BLAST. Hasil penelitian menunjukkan bahwa ditemukan 9 isolat bakteri dari larva ikan tuna sirip kuning yang tumbuh dengan morfologi berbeda. Hasil Analisa BLAST, bakteri tersebut memiliki kemiripan dengan Bacillus cereus, B. methylotrophicus, B. amyloliquefaciens, Pseudomonas plecoglossicida, P. putida dan Vibrio sp. strain voc. Hasil penelitian ini diharapkan dapat menjadi informasi awal tentang bakteri yang ada pada larva ikan tuna sirip kuning.
Co-Authors Fris Johnny Ketut Mahardika Mastuti, Indah Setiadi, Ananto Zafran, Zafran